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The map label for this gene is potH [H]

Identifier: 187928553

GI number: 187928553

Start: 1561436

End: 1562704

Strand: Direct

Name: potH [H]

Synonym: Rpic_1469

Alternate gene names: 187928553

Gene position: 1561436-1562704 (Clockwise)

Preceding gene: 187928552

Following gene: 187928554

Centisome position: 39.6

GC content: 65.01

Gene sequence:

>1269_bases
ATGACGATCGCGGCTGAATCGGTTCCGGAATCGACGGCCAAACTCAAACGCGAATTGAAGAGCGCTGAGGCGCGCAGGCG
TGCGATGGCGCTGGCGCTGGTGGCGCCGCTCGCCATCTTCCTGCTGCTGATCTTTGTCGTGCCGATCGGCGCCCTGCTCA
CGCGCGCCGTGCAAAACCCCGAGGTGGCCGACGCGCTGCCCAAGACAGTCATCGCCCTGAAAGACTGGGACCGCAAGAGC
CCGCCGGCCGACGCCGCCTACGCCGCGCTCGCCGCAGACCTCACCATCGTGAGCGAAGGCGAAGCGATGGGCGCACTGGC
CCGCCGCCTCAACACCGAAATCCCGGGCTTCCGCTCGCTGGTTGCAAAGACCGCGCGCGCCATGCCGCTGGCCGATGATC
AGGGCAAGGCATTGACGCTGACACCCGGCCATATCCGCGCCAAACTCCTCGAAGTCGACGAGCGCTGGGGCGATGTCACG
TATTGGCAGGCCATCGCCAAGAACAGCAGCCGCACGTCGCCGTTCTATCTGCTGGCCGCGCTGGACCACCGGCAGGATGC
CTTCGGCCAGATCGAAGCGACCGACCCCGACGAGCAGATCTACGTGACGGTGTTCATCCGCACCTTCGTCATCAGCGCGA
TGGTGACGTTGTTCGCGCTGCTGCTGGGTTATCCGCTCTCGTACTGGATTGCCTCGCTGCCGGAGCGCCGCGCCAATCTC
GTCATGGTGATGGTGCTGATCCCGTTCTGGACGTCCATCCTCGTGCGCGTGGCGGCGTGGATCGTGCTGCTGCAGACCGA
GGGCCTGATCAACCACGGCCTGATGGACCTCGGCATCATCAAGGAGCCGCTGGCGCTGCTGTTCAACCGCGTGGGCGTGT
ACATCTCGATGACGCACATCCTGCTGCCGTTCATGATCCTGCCGCTGTACAGCGTGATGAAGTCGATCCCGCCCACGTAC
CAGCGCGCGGCAGTGTCGCTGGGCAGCCATCCGTTTGCCGCCTTCTGGCGCGTGTATGTGCCGCAGACGTATCCGGGCAT
CGGCGCCGGCGCGCTGCTGGTGTTCATTCTCGCGCTGGGCTACTACATCACCCCCGCACTGCTGGGCGGGCCGAACGACC
AGATGGTCAGCTACTACGTGGCCTACTTCACCAACGTCACGATCAACTGGGGCATGGCCTGCGCGCTGGGTGCGCTGCTG
TTGCTGGCGACGCTGGTGCTGTATGCCGTGTATGGACGCTTCACGCGGCCCAAGGTTCGCGTTGGTTGA

Upstream 100 bases:

>100_bases
CATAGCGGTGTAACGCAGTGACCGCGCGGATGCCTTGCAGGTTTGGCAAGGCATCCGCCGGACGAATTCCCGTAGCGATG
GCGAGGCAGCATTGAACACC

Downstream 100 bases:

>100_bases
ACGGAGCCCATCGAAAATGAAACTCACCAAACCGATGTTCGCCCCGCACACCTCGCTCATCGAGCGCATTTGGTACTTCG
CGCTGCGCGGCCTGGCCGTG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTIAAESVPESTAKLKRELKSAEARRRAMALALVAPLAIFLLLIFVVPIGALLTRAVQNPEVADALPKTVIALKDWDRKS
PPADAAYAALAADLTIVSEGEAMGALARRLNTEIPGFRSLVAKTARAMPLADDQGKALTLTPGHIRAKLLEVDERWGDVT
YWQAIAKNSSRTSPFYLLAALDHRQDAFGQIEATDPDEQIYVTVFIRTFVISAMVTLFALLLGYPLSYWIASLPERRANL
VMVMVLIPFWTSILVRVAAWIVLLQTEGLINHGLMDLGIIKEPLALLFNRVGVYISMTHILLPFMILPLYSVMKSIPPTY
QRAAVSLGSHPFAAFWRVYVPQTYPGIGAGALLVFILALGYYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGALL
LLATLVLYAVYGRFTRPKVRVG

Sequences:

>Translated_422_residues
MTIAAESVPESTAKLKRELKSAEARRRAMALALVAPLAIFLLLIFVVPIGALLTRAVQNPEVADALPKTVIALKDWDRKS
PPADAAYAALAADLTIVSEGEAMGALARRLNTEIPGFRSLVAKTARAMPLADDQGKALTLTPGHIRAKLLEVDERWGDVT
YWQAIAKNSSRTSPFYLLAALDHRQDAFGQIEATDPDEQIYVTVFIRTFVISAMVTLFALLLGYPLSYWIASLPERRANL
VMVMVLIPFWTSILVRVAAWIVLLQTEGLINHGLMDLGIIKEPLALLFNRVGVYISMTHILLPFMILPLYSVMKSIPPTY
QRAAVSLGSHPFAAFWRVYVPQTYPGIGAGALLVFILALGYYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGALL
LLATLVLYAVYGRFTRPKVRVG
>Mature_421_residues
TIAAESVPESTAKLKRELKSAEARRRAMALALVAPLAIFLLLIFVVPIGALLTRAVQNPEVADALPKTVIALKDWDRKSP
PADAAYAALAADLTIVSEGEAMGALARRLNTEIPGFRSLVAKTARAMPLADDQGKALTLTPGHIRAKLLEVDERWGDVTY
WQAIAKNSSRTSPFYLLAALDHRQDAFGQIEATDPDEQIYVTVFIRTFVISAMVTLFALLLGYPLSYWIASLPERRANLV
MVMVLIPFWTSILVRVAAWIVLLQTEGLINHGLMDLGIIKEPLALLFNRVGVYISMTHILLPFMILPLYSVMKSIPPTYQ
RAAVSLGSHPFAAFWRVYVPQTYPGIGAGALLVFILALGYYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGALLL
LATLVLYAVYGRFTRPKVRVG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=212, Percent_Identity=36.3207547169811, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787369, Length=220, Percent_Identity=32.2727272727273, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1787713, Length=183, Percent_Identity=30.6010928961749, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 46307; Mature: 46176

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAAESVPESTAKLKRELKSAEARRRAMALALVAPLAIFLLLIFVVPIGALLTRAVQNP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVADALPKTVIALKDWDRKSPPADAAYAALAADLTIVSEGEAMGALARRLNTEIPGFRSL
HHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCCHHHHH
VAKTARAMPLADDQGKALTLTPGHIRAKLLEVDERWGDVTYWQAIAKNSSRTSPFYLLAA
HHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEE
LDHRQDAFGQIEATDPDEQIYVTVFIRTFVISAMVTLFALLLGYPLSYWIASLPERRANL
HHCCHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHH
VMVMVLIPFWTSILVRVAAWIVLLQTEGLINHGLMDLGIIKEPLALLFNRVGVYISMTHI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPFMILPLYSVMKSIPPTYQRAAVSLGSHPFAAFWRVYVPQTYPGIGAGALLVFILALG
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
YYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGALLLLATLVLYAVYGRFTRPKVR
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VG
CC
>Mature Secondary Structure 
TIAAESVPESTAKLKRELKSAEARRRAMALALVAPLAIFLLLIFVVPIGALLTRAVQNP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVADALPKTVIALKDWDRKSPPADAAYAALAADLTIVSEGEAMGALARRLNTEIPGFRSL
HHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHEEEECCHHHHHHHHHHCCCCCHHHHH
VAKTARAMPLADDQGKALTLTPGHIRAKLLEVDERWGDVTYWQAIAKNSSRTSPFYLLAA
HHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEE
LDHRQDAFGQIEATDPDEQIYVTVFIRTFVISAMVTLFALLLGYPLSYWIASLPERRANL
HHCCHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHH
VMVMVLIPFWTSILVRVAAWIVLLQTEGLINHGLMDLGIIKEPLALLFNRVGVYISMTHI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPFMILPLYSVMKSIPPTYQRAAVSLGSHPFAAFWRVYVPQTYPGIGAGALLVFILALG
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
YYITPALLGGPNDQMVSYYVAYFTNVTINWGMACALGALLLLATLVLYAVYGRFTRPKVR
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]