BacMap

Definition	Ralstonia pickettii 12J chromosome chromosome 1, complete sequence.
Accession	NC_010682
Length	3,942,557

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The map label for this gene is ligD [H]

Identifier: 187928523

GI number: 187928523

Start: 1524579

End: 1525481

Strand: Reverse

Name: ligD [H]

Synonym: Rpic_1437

Alternate gene names: 187928523

Gene position: 1525481-1524579 (Counterclockwise)

Preceding gene: 187928524

Following gene: 187928522

Centisome position: 38.69

GC content: 63.12

Gene sequence:

>903_bases
ATGAAGCAATTCGCAGGCGGTGTGGCCGTCATCACGGGCGGTGCGTCGGGTTTTGGCAAGGAGTTCGCCAAGATTGGTGC
CGGGTTGGGCATGAAGCTGGTGCTGGCCGATATCCAGCAGGATGCGCTGGATGCCGCCGTGGCCGAATTCTCGGCGCAGG
GCGTGGAAGTGATCGGCCTGCGCGTCGACGTGTCGAAGGTGGAAGACGTGCAAGCGCTGGCCGATGCCGCCATCAAGGCG
TTCGGCAAGGTCAACCTCCTGTTCAACAACGCCGGCGTGGGTGCGGGCGGGCTGATCTGGGAGAACACCGAGCGCGACTG
GGATTGGGTGCTCGGCGTGAACCTGCACGGCGTCATCCACGGTGTACGCATCTTCACGCCGCTGATGCTCGAAGCCGCCA
AGAAAGATCCGTCTTACGAGGGTCATATCGTCAATACGGCGTCGATGGCAGGGCTGCTGAATGCGCCGGCCATGGGCATC
TACAACGTGTCCAAGCACGCGGTGGTGGCGCTGTCCGAATCGCTGTATCAGGACTTGAGCCTGGTCACCGAACAGATCCA
TTGCTCGGTGCTGTGCCCGTACTTCGTGCCGACCGGCATCACGCAGTCTGACCGCAACCGCCCACAGGATCTGGCCAACA
CGGCGCCGCCCACCAAGTCGCAGCTCGTTTCGCGCGCCATGTCGGAAAAGGCGGTCAGCTCCGGCAAGGTCACCGCCGAG
CAGGTCGGCCAGATGACCTTCGATGCGATCCGCAACGCGGGCTTCTACATCTACTCGCACCCACACGCGCTGGCGCCCGT
GCAGCATCGTTTTGAAGACATCGTCTCGCAGCGCAATCCGTCCGATCCGTTTGAAGGCAAGCCAGACGTGCGTGCGCGTC
TGGTCGATGCGCTGCGCGGGTAA

Upstream 100 bases:

>100_bases
CGCGCGTGGGTTATGCCGTGCGCAAGGTCGAGGCGGCAGGCTGACACGGCTTTGCCATCTCGATCGCCTTTCCTACTACA
ACGACATCAGTGAGACACGT

Downstream 100 bases:

>100_bases
CCCCACCTCCACGACTGCCATAACGCCGAGATCGCCATGAACACTGCAACGGCTGCCGCCATCACGGAATCGCAATTCGC
CGATCTGCCCAACGGCACGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKQFAGGVAVITGGASGFGKEFAKIGAGLGMKLVLADIQQDALDAAVAEFSAQGVEVIGLRVDVSKVEDVQALADAAIKA
FGKVNLLFNNAGVGAGGLIWENTERDWDWVLGVNLHGVIHGVRIFTPLMLEAAKKDPSYEGHIVNTASMAGLLNAPAMGI
YNVSKHAVVALSESLYQDLSLVTEQIHCSVLCPYFVPTGITQSDRNRPQDLANTAPPTKSQLVSRAMSEKAVSSGKVTAE
QVGQMTFDAIRNAGFYIYSHPHALAPVQHRFEDIVSQRNPSDPFEGKPDVRARLVDALRG

Sequences:

>Translated_300_residues
MKQFAGGVAVITGGASGFGKEFAKIGAGLGMKLVLADIQQDALDAAVAEFSAQGVEVIGLRVDVSKVEDVQALADAAIKA
FGKVNLLFNNAGVGAGGLIWENTERDWDWVLGVNLHGVIHGVRIFTPLMLEAAKKDPSYEGHIVNTASMAGLLNAPAMGI
YNVSKHAVVALSESLYQDLSLVTEQIHCSVLCPYFVPTGITQSDRNRPQDLANTAPPTKSQLVSRAMSEKAVSSGKVTAE
QVGQMTFDAIRNAGFYIYSHPHALAPVQHRFEDIVSQRNPSDPFEGKPDVRARLVDALRG
>Mature_300_residues
MKQFAGGVAVITGGASGFGKEFAKIGAGLGMKLVLADIQQDALDAAVAEFSAQGVEVIGLRVDVSKVEDVQALADAAIKA
FGKVNLLFNNAGVGAGGLIWENTERDWDWVLGVNLHGVIHGVRIFTPLMLEAAKKDPSYEGHIVNTASMAGLLNAPAMGI
YNVSKHAVVALSESLYQDLSLVTEQIHCSVLCPYFVPTGITQSDRNRPQDLANTAPPTKSQLVSRAMSEKAVSSGKVTAE
QVGQMTFDAIRNAGFYIYSHPHALAPVQHRFEDIVSQRNPSDPFEGKPDVRARLVDALRG

Specific function: Catalyzes the C alpha dehydrogenation of arylglycerol- beta-aryl ether (C alpha alcohol type) (compound IV) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI31542939, Length=202, Percent_Identity=30.6930693069307, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI25282469, Length=254, Percent_Identity=30.7086614173228, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI142976729, Length=227, Percent_Identity=30.8370044052863, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40807363, Length=204, Percent_Identity=29.9019607843137, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI32483357, Length=215, Percent_Identity=27.906976744186, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI20149619, Length=200, Percent_Identity=29, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI33667109, Length=203, Percent_Identity=29.5566502463054, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI210032110, Length=207, Percent_Identity=29.951690821256, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI5031737, Length=200, Percent_Identity=29.5, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI310128830, Length=215, Percent_Identity=26.046511627907, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI310115468, Length=215, Percent_Identity=26.046511627907, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI224922801, Length=173, Percent_Identity=31.2138728323699, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4504505, Length=294, Percent_Identity=24.4897959183673, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI109715829, Length=202, Percent_Identity=26.2376237623762, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI40254992, Length=195, Percent_Identity=27.6923076923077, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI83715985, Length=185, Percent_Identity=29.7297297297297, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4758504, Length=185, Percent_Identity=29.7297297297297, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87082100, Length=213, Percent_Identity=28.6384976525822, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87082160, Length=206, Percent_Identity=28.6407766990291, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789208, Length=228, Percent_Identity=27.1929824561404, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787335, Length=212, Percent_Identity=25.4716981132075, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787905, Length=124, Percent_Identity=34.6774193548387, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789057, Length=186, Percent_Identity=28.494623655914, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790717, Length=200, Percent_Identity=25, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=202, Percent_Identity=32.6732673267327, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI193203115, Length=203, Percent_Identity=33.0049261083744, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17564282, Length=204, Percent_Identity=28.921568627451, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508895, Length=193, Percent_Identity=30.0518134715026, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17568967, Length=246, Percent_Identity=29.2682926829268, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17567345, Length=209, Percent_Identity=30.1435406698565, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17561402, Length=203, Percent_Identity=25.615763546798, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25147288, Length=201, Percent_Identity=27.363184079602, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=231, Percent_Identity=25.5411255411255, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=200, Percent_Identity=27, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=203, Percent_Identity=25.1231527093596, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=206, Percent_Identity=27.1844660194175, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=223, Percent_Identity=28.2511210762332, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=184, Percent_Identity=31.5217391304348, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21355319, Length=204, Percent_Identity=32.843137254902, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24581726, Length=209, Percent_Identity=31.1004784688995, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24640492, Length=247, Percent_Identity=27.1255060728745, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI18859837, Length=209, Percent_Identity=30.1435406698565, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281364892, Length=204, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644339, Length=190, Percent_Identity=29.4736842105263, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639444, Length=196, Percent_Identity=28.0612244897959, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21355631, Length=181, Percent_Identity=30.3867403314917, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221510726, Length=196, Percent_Identity=30.1020408163265, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737361, Length=192, Percent_Identity=29.6875, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357041, Length=181, Percent_Identity=27.0718232044199, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28571526, Length=181, Percent_Identity=28.7292817679558, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364494, Length=263, Percent_Identity=26.2357414448669, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI23397609, Length=194, Percent_Identity=30.4123711340206, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24649181, Length=200, Percent_Identity=27, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116007236, Length=226, Percent_Identity=26.1061946902655, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 31996; Mature: 31996

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQFAGGVAVITGGASGFGKEFAKIGAGLGMKLVLADIQQDALDAAVAEFSAQGVEVIGL
CCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
RVDVSKVEDVQALADAAIKAFGKVNLLFNNAGVGAGGLIWENTERDWDWVLGVNLHGVIH
EEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEECCCCCCCEEEEECCHHHHHH
GVRIFTPLMLEAAKKDPSYEGHIVNTASMAGLLNAPAMGIYNVSKHAVVALSESLYQDLS
HHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHH
LVTEQIHCSVLCPYFVPTGITQSDRNRPQDLANTAPPTKSQLVSRAMSEKAVSSGKVTAE
HHHHHHHHHEECHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHH
QVGQMTFDAIRNAGFYIYSHPHALAPVQHRFEDIVSQRNPSDPFEGKPDVRARLVDALRG
HHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKQFAGGVAVITGGASGFGKEFAKIGAGLGMKLVLADIQQDALDAAVAEFSAQGVEVIGL
CCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
RVDVSKVEDVQALADAAIKAFGKVNLLFNNAGVGAGGLIWENTERDWDWVLGVNLHGVIH
EEEHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEECCCCCCCEEEEECCHHHHHH
GVRIFTPLMLEAAKKDPSYEGHIVNTASMAGLLNAPAMGIYNVSKHAVVALSESLYQDLS
HHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHH
LVTEQIHCSVLCPYFVPTGITQSDRNRPQDLANTAPPTKSQLVSRAMSEKAVSSGKVTAE
HHHHHHHHHEECHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHH
QVGQMTFDAIRNAGFYIYSHPHALAPVQHRFEDIVSQRNPSDPFEGKPDVRARLVDALRG
HHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726; 7764263 [H]