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The map label for this gene is dhaT [H]

Identifier: 187928521

GI number: 187928521

Start: 1522458

End: 1523606

Strand: Direct

Name: dhaT [H]

Synonym: Rpic_1435

Alternate gene names: 187928521

Gene position: 1522458-1523606 (Clockwise)

Preceding gene: 187928517

Following gene: 187928534

Centisome position: 38.62

GC content: 67.19

Gene sequence:

>1149_bases
ATGGCCCTCATCTTCTACCTCACGCACGTTCACCTTGGCTTCGGCACCATCAATGAACTGAAAAGCGAATGCGCACGCGT
CGGCATCCGCCGGCCGATGATCGTGACCGACAAGGGCGTAGCCGCCGCAGGGCTGGCACAGCGCGCCATTGACGCCACGG
GCGGCTTGCCCGTCACCGTGTTCGACGAAACGCCCTCCAACCCAACCGAGGCGATGGTGCTGAAAGCCACCGCACAGTAT
AAAGACGCCGGCTGCGACGGGCTGATCGCCATTGGCGGCGGTTCGTCGATCGACCTGGCCAAGGGCATCGCCATTGCGGC
CACGCACCCGGAACCGCTCACCACCTACGCGACCATCGAGGGCGGCAGCACGAAGATCACGGAAGCGGCAGCGCCGCTGA
TTGCGATTCCCACGACGGCCGGCACCGGCAGCGAGGTGGCGCGCGGCGCGATCATCATCCTGGAAGACGGCCGCAAGCTC
GGTTTCCACTCCTGGCATCTGCTGCCCAAGTCCGCCATCTGCGATGCGGAACTGACGCTCGGTCTGCCGCCCGCGCTGAC
GGCCGCCACTGGCATGGACGCGATCGCGCATTGCATCGAGACCTTCCTGGCCCCGGCGTTCAACCCGCCCGCGGACGGCA
TCGCCCTGGATGGTTTGGAGCGCGCATGGGCCAACATCGAACGCGCCACGCGCGATGGCACTGACCGCGATGCGCGCCTG
AACATGATGAGCGCGTCGATGCAGGGCGCGATGGCGTTTCAGAAGGGCTTGGGGTGCGTGCATTCGTTGTCGCACCCGCT
GGGCGGCGTGAAGGTCGACGGCAAGACTGGGTTGCATCACGGCACGCTGAATGCGGTGGTGCTGCCGGCGGTGCTGCGCT
TCAACGAAACGGCCGAGACGGTGGTGCGCGACAACCGCTACGCCCGCATGCGCCGCGTGATGAACCTGCCGGCGGGCGCC
GACCTTGCCCAGGCCGTGCACGACCTGACGGCGCGCCTGGGTCTGCCGACCGGCCTGCGCCAGATGGGCGTGCCCGAAGA
TGCGCTGGAACACGTCGTGGAAGGCGCCCTGCTCGACCACTGCCACAAGACCAACCCGCGCATCGCCACAGCGGACGACT
ACCGCCGCATGCTGGCGGAGTCGATGTAA

Upstream 100 bases:

>100_bases
AACTGAACGATCGTTCTAATTTGGCTATCATCGGAGTATCCATTGAACCACGCCCGCACGACAAGACGGGCGATGCAGAC
CAACACCCTCTGGAGACAGC

Downstream 100 bases:

>100_bases
CGCGGGCGTTCAGGAAACGAACGACCGGATCAGCGCATTAACGCGCTCCGGTTGTTCGTGCACGACCCAGTGCGTGCCGT
CGGGAATGCGCTCCAGGCGC

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: 1,3-propanediol oxidoreductase; 3-hydroxypropionaldehyde reductase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MALIFYLTHVHLGFGTINELKSECARVGIRRPMIVTDKGVAAAGLAQRAIDATGGLPVTVFDETPSNPTEAMVLKATAQY
KDAGCDGLIAIGGGSSIDLAKGIAIAATHPEPLTTYATIEGGSTKITEAAAPLIAIPTTAGTGSEVARGAIIILEDGRKL
GFHSWHLLPKSAICDAELTLGLPPALTAATGMDAIAHCIETFLAPAFNPPADGIALDGLERAWANIERATRDGTDRDARL
NMMSASMQGAMAFQKGLGCVHSLSHPLGGVKVDGKTGLHHGTLNAVVLPAVLRFNETAETVVRDNRYARMRRVMNLPAGA
DLAQAVHDLTARLGLPTGLRQMGVPEDALEHVVEGALLDHCHKTNPRIATADDYRRMLAESM

Sequences:

>Translated_382_residues
MALIFYLTHVHLGFGTINELKSECARVGIRRPMIVTDKGVAAAGLAQRAIDATGGLPVTVFDETPSNPTEAMVLKATAQY
KDAGCDGLIAIGGGSSIDLAKGIAIAATHPEPLTTYATIEGGSTKITEAAAPLIAIPTTAGTGSEVARGAIIILEDGRKL
GFHSWHLLPKSAICDAELTLGLPPALTAATGMDAIAHCIETFLAPAFNPPADGIALDGLERAWANIERATRDGTDRDARL
NMMSASMQGAMAFQKGLGCVHSLSHPLGGVKVDGKTGLHHGTLNAVVLPAVLRFNETAETVVRDNRYARMRRVMNLPAGA
DLAQAVHDLTARLGLPTGLRQMGVPEDALEHVVEGALLDHCHKTNPRIATADDYRRMLAESM
>Mature_381_residues
ALIFYLTHVHLGFGTINELKSECARVGIRRPMIVTDKGVAAAGLAQRAIDATGGLPVTVFDETPSNPTEAMVLKATAQYK
DAGCDGLIAIGGGSSIDLAKGIAIAATHPEPLTTYATIEGGSTKITEAAAPLIAIPTTAGTGSEVARGAIIILEDGRKLG
FHSWHLLPKSAICDAELTLGLPPALTAATGMDAIAHCIETFLAPAFNPPADGIALDGLERAWANIERATRDGTDRDARLN
MMSASMQGAMAFQKGLGCVHSLSHPLGGVKVDGKTGLHHGTLNAVVLPAVLRFNETAETVVRDNRYARMRRVMNLPAGAD
LAQAVHDLTARLGLPTGLRQMGVPEDALEHVVEGALLDHCHKTNPRIATADDYRRMLAESM

Specific function: Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=393, Percent_Identity=26.7175572519084, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI48994951, Length=380, Percent_Identity=34.7368421052632, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1789163, Length=374, Percent_Identity=33.9572192513369, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI87082107, Length=370, Percent_Identity=32.972972972973, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1787493, Length=389, Percent_Identity=29.5629820051414, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1789386, Length=354, Percent_Identity=29.0960451977401, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17537053, Length=388, Percent_Identity=28.0927835051546, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321181, Length=373, Percent_Identity=35.656836461126, Blast_Score=190, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24657991, Length=419, Percent_Identity=29.8329355608592, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.202 [H]

Molecular weight: Translated: 40137; Mature: 40005

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIFYLTHVHLGFGTINELKSECARVGIRRPMIVTDKGVAAAGLAQRAIDATGGLPVTV
CEEEEEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEE
FDETPSNPTEAMVLKATAQYKDAGCDGLIAIGGGSSIDLAKGIAIAATHPEPLTTYATIE
ECCCCCCCCCEEEEEEEHHHCCCCCCEEEEECCCCCCHHHCCEEEEECCCCCCEEEEEEC
GGSTKITEAAAPLIAIPTTAGTGSEVARGAIIILEDGRKLGFHSWHLLPKSAICDAELTL
CCCCCHHHHCCCEEEECCCCCCCCHHHCCEEEEEECCCCCCCCEEECCCCCCCCCCCEEC
GLPPALTAATGMDAIAHCIETFLAPAFNPPADGIALDGLERAWANIERATRDGTDRDARL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHH
NMMSASMQGAMAFQKGLGCVHSLSHPLGGVKVDGKTGLHHGTLNAVVLPAVLRFNETAET
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCHHHHH
VVRDNRYARMRRVMNLPAGADLAQAVHDLTARLGLPTGLRQMGVPEDALEHVVEGALLDH
HHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHH
CHKTNPRIATADDYRRMLAESM
HHCCCCCEECHHHHHHHHHHCC
>Mature Secondary Structure 
ALIFYLTHVHLGFGTINELKSECARVGIRRPMIVTDKGVAAAGLAQRAIDATGGLPVTV
EEEEEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEE
FDETPSNPTEAMVLKATAQYKDAGCDGLIAIGGGSSIDLAKGIAIAATHPEPLTTYATIE
ECCCCCCCCCEEEEEEEHHHCCCCCCEEEEECCCCCCHHHCCEEEEECCCCCCEEEEEEC
GGSTKITEAAAPLIAIPTTAGTGSEVARGAIIILEDGRKLGFHSWHLLPKSAICDAELTL
CCCCCHHHHCCCEEEECCCCCCCCHHHCCEEEEEECCCCCCCCEEECCCCCCCCCCCEEC
GLPPALTAATGMDAIAHCIETFLAPAFNPPADGIALDGLERAWANIERATRDGTDRDARL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHH
NMMSASMQGAMAFQKGLGCVHSLSHPLGGVKVDGKTGLHHGTLNAVVLPAVLRFNETAET
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCHHHHH
VVRDNRYARMRRVMNLPAGADLAQAVHDLTARLGLPTGLRQMGVPEDALEHVVEGALLDH
HHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHH
CHKTNPRIATADDYRRMLAESM
HHCCCCCEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7721705 [H]