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The map label for this gene is lip2 [H]

Identifier: 187928520

GI number: 187928520

Start: 1521368

End: 1522348

Strand: Reverse

Name: lip2 [H]

Synonym: Rpic_1434

Alternate gene names: 187928520

Gene position: 1522348-1521368 (Counterclockwise)

Preceding gene: 187928522

Following gene: 187928519

Centisome position: 38.61

GC content: 65.55

Gene sequence:

>981_bases
ATGCAGCACCCGTCTCTCGACCTGCACGTCGCCCAGTTGCTCGATCTGGTTGCGCGTGCCAAGCGTCCGCCGCTGAATGC
CCTTGACCCAGCCGACGCCAAGATTGCCTACGAAAAAAGCAGCCCCATCGTCGACATCCCGCCCATCGCGCTGGAAGCTG
TGCATGACCTGACGGTGACGGCGCGCGATGGCCATGCCATCCCTGTCCGCACGTACGCAGCGCGCGAGGCCAGTTGGGCC
GATCCGCTGCCCCTGCTGGTGTATTTCCACGGCGGTGGTTTTACCGTGGGCAGCATCAAGACGCACGACCAGCTTTGCCG
GTCGCTGGCGGCCAAATCGGGGGCGATGGTGTTGTCAGTGGACTACCGGCTTGGGCCCGACTGGAAATTTCCGACCGCAG
CGAACGACGCATTCGACGTGCTGCAGTGGGTGTTCGATGAAGCCCCCACCATGGGCGCTGACCCAGCCCGCATCGCATTC
GGCGGCGACAGCGCGGGCGGGACGCTGGCCGCGATCAGCGCCATCGAGGCACGCAACCGCGGCCTGGCACCTGTGCTGCA
GCTGCTGATCTACCCCGGCACCACCGCGCGTGAATCCACGCCGTCGCACCGTGAGTTTGCCGAGGGCTACCTGCTGACGC
AGGACATGATCCACTGGTTCTTCTCTCAATACCTGCGCAGTGATGCCGACCGCGACGACTGGCGTTTCGCCCCGCTCGAT
GCAGGCGGCCATGGCGCGGACGTGACCGGCGTCTGCCCTGCCTGGATTGCCGTGGCCGGCTTCGACCCGATCCGCGATGC
GGGCATCGGCTACGCCAACAAGCTGCGCGCGGCAGAAGTGCCCGTCGAGCTGAAACTGTACGAAGGCATGATTCACGACT
TCTTCAAGATCGGGCGCTTCGTGCCGGCTGTGGAAGACGCGCACCGCGATGCCGCCGACGCGCTACGCCGAGCCTTCGGC
ACCGAGACCGAATCCCTATAA

Upstream 100 bases:

>100_bases
AGAGGGTGTTGGTCTGCATCGCCCGTCTTGTCGTGCGGGCGTGGTTCAATGGATACTCCGATGATAGCCAAATTAGAACG
ATCGTTCAGTTCCCGCCCTC

Downstream 100 bases:

>100_bases
CGAGAGGAGACCGCATGGCGATCGAGGATTTCCGTCACAGCACGCCGCTGCGCGTACGCTGGGCCGAAGTCGATGCGCAG
AGCATCGTCTTCAACGGCCA

Product: alpha/beta hydrolase fold-3 domain-containing protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MQHPSLDLHVAQLLDLVARAKRPPLNALDPADAKIAYEKSSPIVDIPPIALEAVHDLTVTARDGHAIPVRTYAAREASWA
DPLPLLVYFHGGGFTVGSIKTHDQLCRSLAAKSGAMVLSVDYRLGPDWKFPTAANDAFDVLQWVFDEAPTMGADPARIAF
GGDSAGGTLAAISAIEARNRGLAPVLQLLIYPGTTARESTPSHREFAEGYLLTQDMIHWFFSQYLRSDADRDDWRFAPLD
AGGHGADVTGVCPAWIAVAGFDPIRDAGIGYANKLRAAEVPVELKLYEGMIHDFFKIGRFVPAVEDAHRDAADALRRAFG
TETESL

Sequences:

>Translated_326_residues
MQHPSLDLHVAQLLDLVARAKRPPLNALDPADAKIAYEKSSPIVDIPPIALEAVHDLTVTARDGHAIPVRTYAAREASWA
DPLPLLVYFHGGGFTVGSIKTHDQLCRSLAAKSGAMVLSVDYRLGPDWKFPTAANDAFDVLQWVFDEAPTMGADPARIAF
GGDSAGGTLAAISAIEARNRGLAPVLQLLIYPGTTARESTPSHREFAEGYLLTQDMIHWFFSQYLRSDADRDDWRFAPLD
AGGHGADVTGVCPAWIAVAGFDPIRDAGIGYANKLRAAEVPVELKLYEGMIHDFFKIGRFVPAVEDAHRDAADALRRAFG
TETESL
>Mature_326_residues
MQHPSLDLHVAQLLDLVARAKRPPLNALDPADAKIAYEKSSPIVDIPPIALEAVHDLTVTARDGHAIPVRTYAAREASWA
DPLPLLVYFHGGGFTVGSIKTHDQLCRSLAAKSGAMVLSVDYRLGPDWKFPTAANDAFDVLQWVFDEAPTMGADPARIAF
GGDSAGGTLAAISAIEARNRGLAPVLQLLIYPGTTARESTPSHREFAEGYLLTQDMIHWFFSQYLRSDADRDDWRFAPLD
AGGHGADVTGVCPAWIAVAGFDPIRDAGIGYANKLRAAEVPVELKLYEGMIHDFFKIGRFVPAVEDAHRDAADALRRAFG
TETESL

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157041239, Length=184, Percent_Identity=35.8695652173913, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI206597554, Length=180, Percent_Identity=36.6666666666667, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI68299767, Length=192, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI21328446, Length=111, Percent_Identity=36.036036036036, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI68051721, Length=270, Percent_Identity=25.1851851851852, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI61966717, Length=207, Percent_Identity=28.9855072463768, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI226423947, Length=278, Percent_Identity=24.1007194244604, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1786682, Length=263, Percent_Identity=31.5589353612167, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17567059, Length=267, Percent_Identity=27.3408239700375, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI72001146, Length=148, Percent_Identity=33.1081081081081, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=170, Percent_Identity=30, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540028, Length=256, Percent_Identity=25.78125, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24656084, Length=133, Percent_Identity=30.8270676691729, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656076, Length=133, Percent_Identity=30.8270676691729, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130169, Length=133, Percent_Identity=30.8270676691729, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 35355; Mature: 35355

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHPSLDLHVAQLLDLVARAKRPPLNALDPADAKIAYEKSSPIVDIPPIALEAVHDLTVT
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEECCCHHHHHHHHEEEE
ARDGHAIPVRTYAAREASWADPLPLLVYFHGGGFTVGSIKTHDQLCRSLAAKSGAMVLSV
ECCCCEEEEEHHHHCCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHHCCCCEEEEE
DYRLGPDWKFPTAANDAFDVLQWVFDEAPTMGADPARIAFGGDSAGGTLAAISAIEARNR
ECEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCC
GLAPVLQLLIYPGTTARESTPSHREFAEGYLLTQDMIHWFFSQYLRSDADRDDWRFAPLD
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
AGGHGADVTGVCPAWIAVAGFDPIRDAGIGYANKLRAAEVPVELKLYEGMIHDFFKIGRF
CCCCCCCCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
VPAVEDAHRDAADALRRAFGTETESL
CCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQHPSLDLHVAQLLDLVARAKRPPLNALDPADAKIAYEKSSPIVDIPPIALEAVHDLTVT
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEECCCHHHHHHHHEEEE
ARDGHAIPVRTYAAREASWADPLPLLVYFHGGGFTVGSIKTHDQLCRSLAAKSGAMVLSV
ECCCCEEEEEHHHHCCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHHCCCCEEEEE
DYRLGPDWKFPTAANDAFDVLQWVFDEAPTMGADPARIAFGGDSAGGTLAAISAIEARNR
ECEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCC
GLAPVLQLLIYPGTTARESTPSHREFAEGYLLTQDMIHWFFSQYLRSDADRDDWRFAPLD
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
AGGHGADVTGVCPAWIAVAGFDPIRDAGIGYANKLRAAEVPVELKLYEGMIHDFFKIGRF
CCCCCCCCCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
VPAVEDAHRDAADALRRAFGTETESL
CCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]