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The map label for this gene is yngG [H]

Identifier: 187928465

GI number: 187928465

Start: 1474545

End: 1475540

Strand: Reverse

Name: yngG [H]

Synonym: Rpic_1378

Alternate gene names: 187928465

Gene position: 1475540-1474545 (Counterclockwise)

Preceding gene: 187928466

Following gene: 187928464

Centisome position: 37.43

GC content: 65.46

Gene sequence:

>996_bases
ATGTCCAGCACGGGGAACGCAGTGGTACTCAACGGTCAAGGGAAAAGGGTGTTCATCCAGGAAGTCGCCACGCGCGATGG
CTTTCAGATTGAACCGCGCATGGTTCCGACCCAAGACAAGATCGCGCTTATCGACCGGCTGAGCCAATGCGGGCTGAGCA
AGATCGAAGTCACCTCGTTCACCTCGCCCAAGGCCATCCCGACGCTGGCCGACGCGGAAGACGTGATGCGCGGTATCGCG
CGCGTGCCCGGCGTCACTTACGCCGCGCTCGTGCCGAATATGCGAGGCGCCGAACGTGCGGTGGACTGCGATGTCGACGA
GTTGAACCTCGTGATGTCGGTAAGCGAAACGCACAACCTCGCCAACCTGCGCATGACGCCGGGGCAGTCGTTCCAGCAGG
CTGCGCAGGTCGCGGCGGCAGTCCGTTCGAGCCGCACGTCGCTCAACCTTTCGCTCTCATGCGCGTTCGGCTGTCCGATG
GAAGGCCAGATCCCCACTGACACCGTGCTGACCTGGGCGGCACGGGCCGTCGAAGCGGGCATCGGCGGCATCACGCTGTG
CGACACCACCGGCATGGCCTGGCCCAGCCAGGTGTTCGCGCGGTGCAGTGCATTCCGGCTGGCGTTCCCCCAGACCACGC
TGACCGTGCACTTGCACAACACGCGCGGCATGGCACTTGCCAACGTGCTGGCCGCGCTGCAGGCGGGAGTCGATCGCTTC
GATGGCGCGCTCGGCGGCCTGGGAGGCTGCCCCTACGCGCCCGGTGCCTCGGGCAACGTCTGCACCGAAGACATGGTGCA
CATGCTTGAACTCCTGAACTACGACACTGGCACCAACCTTGGCGCCCTGCTGGAGGCTTCGCGACTGTTGCCGGGCCTCA
TCGGTCATGACACCAACGGTCAGGTCGCCAAGGCCGGACGCACCCACGATCTGCACCCCACGCCCGACTATGTTGAGACG
GCCCGGCAGCACGCCCTGTCGAAAGCACCGGCTTGA

Upstream 100 bases:

>100_bases
AGCGCAGAAAACTAGCGCGAGCCATCTGGTTCGGAGAAGGCTCACGTCCTGATTCTTCAATTGCGCGCTGGCTGCCTGCG
CAACACACTGCAGTTCCAAC

Downstream 100 bases:

>100_bases
GTTTCTTGACGGCCGGTCGTCATGGGTTCCGTGCCGTTCGGTATGATCCCGCCTGTAATCATTTGAATACAGGAGGACAT
CATGGGCGAAGCCAAACGAC

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSSTGNAVVLNGQGKRVFIQEVATRDGFQIEPRMVPTQDKIALIDRLSQCGLSKIEVTSFTSPKAIPTLADAEDVMRGIA
RVPGVTYAALVPNMRGAERAVDCDVDELNLVMSVSETHNLANLRMTPGQSFQQAAQVAAAVRSSRTSLNLSLSCAFGCPM
EGQIPTDTVLTWAARAVEAGIGGITLCDTTGMAWPSQVFARCSAFRLAFPQTTLTVHLHNTRGMALANVLAALQAGVDRF
DGALGGLGGCPYAPGASGNVCTEDMVHMLELLNYDTGTNLGALLEASRLLPGLIGHDTNGQVAKAGRTHDLHPTPDYVET
ARQHALSKAPA

Sequences:

>Translated_331_residues
MSSTGNAVVLNGQGKRVFIQEVATRDGFQIEPRMVPTQDKIALIDRLSQCGLSKIEVTSFTSPKAIPTLADAEDVMRGIA
RVPGVTYAALVPNMRGAERAVDCDVDELNLVMSVSETHNLANLRMTPGQSFQQAAQVAAAVRSSRTSLNLSLSCAFGCPM
EGQIPTDTVLTWAARAVEAGIGGITLCDTTGMAWPSQVFARCSAFRLAFPQTTLTVHLHNTRGMALANVLAALQAGVDRF
DGALGGLGGCPYAPGASGNVCTEDMVHMLELLNYDTGTNLGALLEASRLLPGLIGHDTNGQVAKAGRTHDLHPTPDYVET
ARQHALSKAPA
>Mature_330_residues
SSTGNAVVLNGQGKRVFIQEVATRDGFQIEPRMVPTQDKIALIDRLSQCGLSKIEVTSFTSPKAIPTLADAEDVMRGIAR
VPGVTYAALVPNMRGAERAVDCDVDELNLVMSVSETHNLANLRMTPGQSFQQAAQVAAAVRSSRTSLNLSLSCAFGCPME
GQIPTDTVLTWAARAVEAGIGGITLCDTTGMAWPSQVFARCSAFRLAFPQTTLTVHLHNTRGMALANVLAALQAGVDRFD
GALGGLGGCPYAPGASGNVCTEDMVHMLELLNYDTGTNLGALLEASRLLPGLIGHDTNGQVAKAGRTHDLHPTPDYVETA
RQHALSKAPA

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=291, Percent_Identity=43.298969072165, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI109150422, Length=296, Percent_Identity=41.2162162162162, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI109150427, Length=296, Percent_Identity=41.2162162162162, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI260654708, Length=122, Percent_Identity=48.3606557377049, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786261, Length=295, Percent_Identity=23.3898305084746, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510563, Length=291, Percent_Identity=35.0515463917526, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24582381, Length=292, Percent_Identity=35.2739726027397, Blast_Score=198, Evalue=4e-51,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 34912; Mature: 34781

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTGNAVVLNGQGKRVFIQEVATRDGFQIEPRMVPTQDKIALIDRLSQCGLSKIEVTSF
CCCCCCEEEECCCCCEEEEEHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEECC
TSPKAIPTLADAEDVMRGIARVPGVTYAALVPNMRGAERAVDCDVDELNLVMSVSETHNL
CCCCCCCCCCCHHHHHHHHHHCCCCEEEHHCCCCCCCCHHCCCCHHHHHEEEEEHHCCCC
ANLRMTPGQSFQQAAQVAAAVRSSRTSLNLSLSCAFGCPMEGQIPTDTVLTWAARAVEAG
EEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
IGGITLCDTTGMAWPSQVFARCSAFRLAFPQTTLTVHLHNTRGMALANVLAALQAGVDRF
CCCEEEECCCCCCCHHHHHHHHHHHEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHH
DGALGGLGGCPYAPGASGNVCTEDMVHMLELLNYDTGTNLGALLEASRLLPGLIGHDTNG
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHCCCCCC
QVAKAGRTHDLHPTPDYVETARQHALSKAPA
CEEECCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSTGNAVVLNGQGKRVFIQEVATRDGFQIEPRMVPTQDKIALIDRLSQCGLSKIEVTSF
CCCCCEEEECCCCCEEEEEHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEECC
TSPKAIPTLADAEDVMRGIARVPGVTYAALVPNMRGAERAVDCDVDELNLVMSVSETHNL
CCCCCCCCCCCHHHHHHHHHHCCCCEEEHHCCCCCCCCHHCCCCHHHHHEEEEEHHCCCC
ANLRMTPGQSFQQAAQVAAAVRSSRTSLNLSLSCAFGCPMEGQIPTDTVLTWAARAVEAG
EEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHC
IGGITLCDTTGMAWPSQVFARCSAFRLAFPQTTLTVHLHNTRGMALANVLAALQAGVDRF
CCCEEEECCCCCCCHHHHHHHHHHHEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHH
DGALGGLGGCPYAPGASGNVCTEDMVHMLELLNYDTGTNLGALLEASRLLPGLIGHDTNG
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHCCCCCC
QVAKAGRTHDLHPTPDYVETARQHALSKAPA
CEEECCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]