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The map label for this gene is cysL [H]

Identifier: 187928466

GI number: 187928466

Start: 1475626

End: 1476525

Strand: Reverse

Name: cysL [H]

Synonym: Rpic_1379

Alternate gene names: 187928466

Gene position: 1476525-1475626 (Counterclockwise)

Preceding gene: 187928471

Following gene: 187928465

Centisome position: 37.45

GC content: 64.89

Gene sequence:

>900_bases
ATGCGTTTCGATCTGGAATCGCTGCAGGTATTGGTCTCGGTAATCGAGGAAGGCAGCCTGGCGGCGGCTTCCGAGCGGCA
GCACATCGTGCCTTCGGCCATCAGCCGACGCATTGCGGAGCTGGAAAACGACGCCGGCACCGCCCTGCTCTATCGGCACA
GCCGTGGCGTGGAGCCCACCCCGGCGGGCTCGGCGTTGTACTACCGGGCCAAGCGCCTGATCGATCAGATGCAGGAAATC
TCGTCGGAAATGAGCGAGTACGCCCAGGGCGTGCGGGGGCACGTGCGCATGCACGTCAACTTTTCGACCATGGTCCAGTA
CCTGCCGGGCGACCTGTCCTCGTTCCTGTCGATCAACCCCGACGTGAAGATCGACCTGATGGAGAAAAGCAGCAGCCAAG
TCCTGCGCTCGGTCGAAGCGGGTACCGCTGACATCGGCATCTGCAGCCAGCCGGAAGCTGCACCGGGGCTGGAAGTACGG
CCGTATCACGTCGATAGGCTCGTCCTGATCGTGCCGCACGACCACCCGATCGGCGCGGCGCGCCACCTGCGCTTTGCCGA
TACCCTGGACTATGACTACGTGAGCATGCCGCTGGGCGCGTCAATCTCCACGCTCTGCGCGCAGAGCGCCGAGCAGGCCG
GCAAGCAGCACAAGATCCGCATCCAGGTGACCAGCTTCGAGGGGCTGCGCAACATGGTGGCGGCAGGCCTTGGCATCGGC
CTGCTGCCCAAGGCCAGTGTCAAGCCCTATCTGCGCAACAGCACGCTGCGCATGGTCGAGCTGGATGAGACGTGGGCACA
GCGCCCGCTGTCTGTCATCACGCGGGGCTACGCGGCGCTGCCGGTGCCGGCCCGGCTGCTGATCGACCATCTGGTATCCC
AGGCCAGCGCAGAAAACTAG

Upstream 100 bases:

>100_bases
ATGCATCATGGACACTTCTCGCCAGACCAAAAAACGCTAAATAATCAAGCGAGCCATCTCCAAAGGAGAAGGCTTCAACA
CGAAGGGGGAATGGACGACC

Downstream 100 bases:

>100_bases
CGCGAGCCATCTGGTTCGGAGAAGGCTCACGTCCTGATTCTTCAATTGCGCGCTGGCTGCCTGCGCAACACACTGCAGTT
CCAACATGTCCAGCACGGGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRFDLESLQVLVSVIEEGSLAAASERQHIVPSAISRRIAELENDAGTALLYRHSRGVEPTPAGSALYYRAKRLIDQMQEI
SSEMSEYAQGVRGHVRMHVNFSTMVQYLPGDLSSFLSINPDVKIDLMEKSSSQVLRSVEAGTADIGICSQPEAAPGLEVR
PYHVDRLVLIVPHDHPIGAARHLRFADTLDYDYVSMPLGASISTLCAQSAEQAGKQHKIRIQVTSFEGLRNMVAAGLGIG
LLPKASVKPYLRNSTLRMVELDETWAQRPLSVITRGYAALPVPARLLIDHLVSQASAEN

Sequences:

>Translated_299_residues
MRFDLESLQVLVSVIEEGSLAAASERQHIVPSAISRRIAELENDAGTALLYRHSRGVEPTPAGSALYYRAKRLIDQMQEI
SSEMSEYAQGVRGHVRMHVNFSTMVQYLPGDLSSFLSINPDVKIDLMEKSSSQVLRSVEAGTADIGICSQPEAAPGLEVR
PYHVDRLVLIVPHDHPIGAARHLRFADTLDYDYVSMPLGASISTLCAQSAEQAGKQHKIRIQVTSFEGLRNMVAAGLGIG
LLPKASVKPYLRNSTLRMVELDETWAQRPLSVITRGYAALPVPARLLIDHLVSQASAEN
>Mature_299_residues
MRFDLESLQVLVSVIEEGSLAAASERQHIVPSAISRRIAELENDAGTALLYRHSRGVEPTPAGSALYYRAKRLIDQMQEI
SSEMSEYAQGVRGHVRMHVNFSTMVQYLPGDLSSFLSINPDVKIDLMEKSSSQVLRSVEAGTADIGICSQPEAAPGLEVR
PYHVDRLVLIVPHDHPIGAARHLRFADTLDYDYVSMPLGASISTLCAQSAEQAGKQHKIRIQVTSFEGLRNMVAAGLGIG
LLPKASVKPYLRNSTLRMVELDETWAQRPLSVITRGYAALPVPARLLIDHLVSQASAEN

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=280, Percent_Identity=24.2857142857143, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=25.6198347107438, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=25.1012145748988, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788887, Length=244, Percent_Identity=26.2295081967213, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788748, Length=304, Percent_Identity=28.2894736842105, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=22.0883534136546, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790208, Length=247, Percent_Identity=24.2914979757085, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787949, Length=276, Percent_Identity=24.2753623188406, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786713, Length=260, Percent_Identity=25.7692307692308, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788296, Length=213, Percent_Identity=24.4131455399061, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081904, Length=295, Percent_Identity=23.3898305084746, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI157672245, Length=270, Percent_Identity=23.3333333333333, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790262, Length=302, Percent_Identity=24.5033112582781, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788297, Length=260, Percent_Identity=22.6923076923077, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=24.5059288537549, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=24.4147157190635, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=279, Percent_Identity=23.6559139784946, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787601, Length=278, Percent_Identity=21.2230215827338, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789204, Length=242, Percent_Identity=23.9669421487603, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32765; Mature: 32765

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDLESLQVLVSVIEEGSLAAASERQHIVPSAISRRIAELENDAGTALLYRHSRGVEPT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
PAGSALYYRAKRLIDQMQEISSEMSEYAQGVRGHVRMHVNFSTMVQYLPGDLSSFLSINP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHCCCCHHHHHCCCC
DVKIDLMEKSSSQVLRSVEAGTADIGICSQPEAAPGLEVRPYHVDRLVLIVPHDHPIGAA
CEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCEEECEEEEEECCCCCCCHH
RHLRFADTLDYDYVSMPLGASISTLCAQSAEQAGKQHKIRIQVTSFEGLRNMVAAGLGIG
HHEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHCCCC
LLPKASVKPYLRNSTLRMVELDETWAQRPLSVITRGYAALPVPARLLIDHLVSQASAEN
CCCCCCCCHHHCCCEEEEEEECHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRFDLESLQVLVSVIEEGSLAAASERQHIVPSAISRRIAELENDAGTALLYRHSRGVEPT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
PAGSALYYRAKRLIDQMQEISSEMSEYAQGVRGHVRMHVNFSTMVQYLPGDLSSFLSINP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHCCCCHHHHHCCCC
DVKIDLMEKSSSQVLRSVEAGTADIGICSQPEAAPGLEVRPYHVDRLVLIVPHDHPIGAA
CEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCEEECEEEEEECCCCCCCHH
RHLRFADTLDYDYVSMPLGASISTLCAQSAEQAGKQHKIRIQVTSFEGLRNMVAAGLGIG
HHEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHCCCC
LLPKASVKPYLRNSTLRMVELDETWAQRPLSVITRGYAALPVPARLLIDHLVSQASAEN
CCCCCCCCHHHCCCEEEEEEECHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]