Click here to switch to the map view.

The map label for this gene is bioD

Identifier: 187928460

GI number: 187928460

Start: 1471850

End: 1472557

Strand: Direct

Name: bioD

Synonym: Rpic_1373

Alternate gene names: 187928460

Gene position: 1471850-1472557 (Clockwise)

Preceding gene: 187928459

Following gene: 187928461

Centisome position: 37.33

GC content: 67.37

Gene sequence:

>708_bases
ATGCCCGCACGCTTCGATTGCTTTGTCACCGGCACCGATACGGAGATCGGCAAGACGCTCGTCAGCGCGGTATTGCTGAC
CCGGCTCGCCGACGCCGGCTACCGTGCCGCGGGCCTCAAGCCGATTGCGGCAGGCACGCTGGCGGGCGCGCCCACGCGCA
CCAACGAAGACGTCGAGCAACTGCGCGCCGCCGCGTCAGTCAATCTGCCGTTCGACACCGTTTGCCCATGGTTGCTCGAC
GCGCCGATGTCTCCGCATTTGGCTGCCGCGCGCGAAGGCGTGACGATCACGCTGCCGCCGATTCTCGATGCGTTTGCACA
CGCCAGATCGTTGGCGGATGCCGTTGTGGTGGAGGGCGTTGGTGGTTTTCGCGTGCCGCTGTCGGACGACTTTGATACGG
CTGAGATGGCCGTGGCGTTGGGCTTGCCGGTGGTGCTGGTTGTCGGGTTGCGCCTAGGCTGCCTGAATCACGCCGCCCTT
ACTGCCGAGGCGATTGCCGCGCGCGGGCTGCATCTGGCCGGCTGGGTCGGCAACGTGGTCGACCCGTCCATGGCGGGGCT
TGACGACAATGTCGCCACGCTGCGCCGCTGGATCAACGCACCGCATCTGGGCACGATTCCGCGATTGCCGCACGCCGATG
CGCGGCTGGCCGCGGCCCACGTCGACATCGCACCGCTACTCGCGCGCACTGCTGAAACGCGGCGGTAA

Upstream 100 bases:

>100_bases
ATCACGCTGTCAGCGGCACATACCGATGCAGACATCGAACGGCTTGCGCAGGCGCTGGAAGCGGCCGGCCAATCGCTGCA
GCCGGCCAAGGCAGCCTGAT

Downstream 100 bases:

>100_bases
ACTACGCGCTTCACTCAGTGCAACAGGGACCATTGTGATGCGGGCGATGCGTTGGCTGGCGGTAGCGATTGTCGTGGCGG
CGACGGCAGGATGTGCAGTA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MPARFDCFVTGTDTEIGKTLVSAVLLTRLADAGYRAAGLKPIAAGTLAGAPTRTNEDVEQLRAAASVNLPFDTVCPWLLD
APMSPHLAAAREGVTITLPPILDAFAHARSLADAVVVEGVGGFRVPLSDDFDTAEMAVALGLPVVLVVGLRLGCLNHAAL
TAEAIAARGLHLAGWVGNVVDPSMAGLDDNVATLRRWINAPHLGTIPRLPHADARLAAAHVDIAPLLARTAETRR

Sequences:

>Translated_235_residues
MPARFDCFVTGTDTEIGKTLVSAVLLTRLADAGYRAAGLKPIAAGTLAGAPTRTNEDVEQLRAAASVNLPFDTVCPWLLD
APMSPHLAAAREGVTITLPPILDAFAHARSLADAVVVEGVGGFRVPLSDDFDTAEMAVALGLPVVLVVGLRLGCLNHAAL
TAEAIAARGLHLAGWVGNVVDPSMAGLDDNVATLRRWINAPHLGTIPRLPHADARLAAAHVDIAPLLARTAETRR
>Mature_234_residues
PARFDCFVTGTDTEIGKTLVSAVLLTRLADAGYRAAGLKPIAAGTLAGAPTRTNEDVEQLRAAASVNLPFDTVCPWLLDA
PMSPHLAAAREGVTITLPPILDAFAHARSLADAVVVEGVGGFRVPLSDDFDTAEMAVALGLPVVLVVGLRLGCLNHAALT
AEAIAARGLHLAGWVGNVVDPSMAGLDDNVATLRRWINAPHLGTIPRLPHADARLAAAHVDIAPLLARTAETRR

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=209, Percent_Identity=44.9760765550239, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI87081944, Length=226, Percent_Identity=37.6106194690265, Blast_Score=144, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6324385, Length=182, Percent_Identity=35.1648351648352, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_RALPJ (B2UBJ4)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898947.1
- ProteinModelPortal:   B2UBJ4
- GeneID:   6286985
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1373
- HOGENOM:   HBG650065
- OMA:   TRTNEDV
- ProtClustDB:   PRK00090
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 24488; Mature: 24357

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARFDCFVTGTDTEIGKTLVSAVLLTRLADAGYRAAGLKPIAAGTLAGAPTRTNEDVEQ
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCHHHHHH
LRAAASVNLPFDTVCPWLLDAPMSPHLAAAREGVTITLPPILDAFAHARSLADAVVVEGV
HHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
GGFRVPLSDDFDTAEMAVALGLPVVLVVGLRLGCLNHAALTAEAIAARGLHLAGWVGNVV
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
DPSMAGLDDNVATLRRWINAPHLGTIPRLPHADARLAAAHVDIAPLLARTAETRR
CCHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PARFDCFVTGTDTEIGKTLVSAVLLTRLADAGYRAAGLKPIAAGTLAGAPTRTNEDVEQ
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCHHHHHH
LRAAASVNLPFDTVCPWLLDAPMSPHLAAAREGVTITLPPILDAFAHARSLADAVVVEGV
HHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
GGFRVPLSDDFDTAEMAVALGLPVVLVVGLRLGCLNHAALTAEAIAARGLHLAGWVGNVV
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
DPSMAGLDDNVATLRRWINAPHLGTIPRLPHADARLAAAHVDIAPLLARTAETRR
CCHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA