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The map label for this gene is bioF

Identifier: 187928459

GI number: 187928459

Start: 1470601

End: 1471848

Strand: Direct

Name: bioF

Synonym: Rpic_1372

Alternate gene names: 187928459

Gene position: 1470601-1471848 (Clockwise)

Preceding gene: 187928458

Following gene: 187928460

Centisome position: 37.3

GC content: 66.75

Gene sequence:

>1248_bases
ATGCGATTGCTTGACGACCTGCAGGCGGGCCTAGCTGCTATCGACGCCGCGCACCTGCGCCGCGTGCGCCGCACCGCGTA
CAGCCCGACCGACCGCAGCCAGCGCATCAGCGTGCCCGGTGAAGCGCCGCGCGACATTCTCGGCTTTTGCGGCAACGATT
ATCTCGGCCTGGCAGCGCATCCGGCCTTGGTCGAGGCGATGACGCAGGGCACACAGCAATATGGCTTCGGCAGCGGCGCA
TCGCACCTGGTCAGCGGCCATTCGATCGCGCACGCGCGGCTGGAAGCACGCATGGCTGCGCTGCAAGGCGAACACATTCC
CGAGGCGGATGCGCTGTTCTTCTGCACGGGCTACATGGCCAATCTGGCCGTGGTGAGTGCCATGGCGCAGGCTGGCGGCA
TCCGGCCGGCGGAGGAATGCACGATCTTTTCCGATGCGCTGAACCACGCTTCGCTCATCGACGGTGCGCGGTTGTCGCGG
GCGCCGATCAAGGTCTATCCGCACGTCGACCTCGCTGCGCTGGAAGCGCTTCTGGCCGCGAGCACCAGTCGCAACAAGCT
GATCGTCACGGATGGCGTGTTCAGCATGGATGGCGACATCGCACCGCTGCCCGAACTGCTTGCGCTGGCCGAACGCTACG
ACGCCTGGCTGATCGTGGACGACGCCCATGGTCTGGGCGTGCTGGGCGAGAACGGTGCGGGTGTGCTGTCTCATTTCGGG
CTGCACTCACAGCGCATCATCTATGTCGGCACGTTCGGCAAGGCTGCCGGCGGCTCGGGCGCGGCCATCGTCGGACATCG
GCTGGTGATTGACTGGCTGGTGCAGCGCGCACGCACGTACATCTTCACAACGGCCACGCCGCCGGGCATTGCGTGTGCGG
TGGAAGCGGCATTGGACCTGATCGCAAGCGAGGAGGGGGATGAACGCCGCGCCCGCCTCGATCGCCACATCGCCGTGTGG
AGTGCGCACGCGCAGCGTTTGGCTGCGCGCTTCGGCTGGCAATGGATGCCATCGCCCACGGCCATTCAGCCGATCGTGAT
TGGCGAGAACGCACCCGCATTGGCGCTGGCCGCCGCGCTCGAACACGAAGGCATCCGCATCGCCGCGATTCGCCCGCCGA
CGGTGCCTGCAGGAACGGCACGCTTGCGCATCACGCTGTCAGCGGCACATACCGATGCAGACATCGAACGGCTTGCGCAG
GCGCTGGAAGCGGCCGGCCAATCGCTGCAGCCGGCCAAGGCAGCCTGA

Upstream 100 bases:

>100_bases
GTGCTGAGCGATGAAGAGACCGACACGCTGGTCGAACGCACGCTGCAGACGCTTGAAGACACGCTGGCCCAAGCCACGGG
CGAAACAGAGGGACACACCT

Downstream 100 bases:

>100_bases
TATGCCCGCACGCTTCGATTGCTTTGTCACCGGCACCGATACGGAGATCGGCAAGACGCTCGTCAGCGCGGTATTGCTGA
CCCGGCTCGCCGACGCCGGC

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MRLLDDLQAGLAAIDAAHLRRVRRTAYSPTDRSQRISVPGEAPRDILGFCGNDYLGLAAHPALVEAMTQGTQQYGFGSGA
SHLVSGHSIAHARLEARMAALQGEHIPEADALFFCTGYMANLAVVSAMAQAGGIRPAEECTIFSDALNHASLIDGARLSR
APIKVYPHVDLAALEALLAASTSRNKLIVTDGVFSMDGDIAPLPELLALAERYDAWLIVDDAHGLGVLGENGAGVLSHFG
LHSQRIIYVGTFGKAAGGSGAAIVGHRLVIDWLVQRARTYIFTTATPPGIACAVEAALDLIASEEGDERRARLDRHIAVW
SAHAQRLAARFGWQWMPSPTAIQPIVIGENAPALALAAALEHEGIRIAAIRPPTVPAGTARLRITLSAAHTDADIERLAQ
ALEAAGQSLQPAKAA

Sequences:

>Translated_415_residues
MRLLDDLQAGLAAIDAAHLRRVRRTAYSPTDRSQRISVPGEAPRDILGFCGNDYLGLAAHPALVEAMTQGTQQYGFGSGA
SHLVSGHSIAHARLEARMAALQGEHIPEADALFFCTGYMANLAVVSAMAQAGGIRPAEECTIFSDALNHASLIDGARLSR
APIKVYPHVDLAALEALLAASTSRNKLIVTDGVFSMDGDIAPLPELLALAERYDAWLIVDDAHGLGVLGENGAGVLSHFG
LHSQRIIYVGTFGKAAGGSGAAIVGHRLVIDWLVQRARTYIFTTATPPGIACAVEAALDLIASEEGDERRARLDRHIAVW
SAHAQRLAARFGWQWMPSPTAIQPIVIGENAPALALAAALEHEGIRIAAIRPPTVPAGTARLRITLSAAHTDADIERLAQ
ALEAAGQSLQPAKAA
>Mature_415_residues
MRLLDDLQAGLAAIDAAHLRRVRRTAYSPTDRSQRISVPGEAPRDILGFCGNDYLGLAAHPALVEAMTQGTQQYGFGSGA
SHLVSGHSIAHARLEARMAALQGEHIPEADALFFCTGYMANLAVVSAMAQAGGIRPAEECTIFSDALNHASLIDGARLSR
APIKVYPHVDLAALEALLAASTSRNKLIVTDGVFSMDGDIAPLPELLALAERYDAWLIVDDAHGLGVLGENGAGVLSHFG
LHSQRIIYVGTFGKAAGGSGAAIVGHRLVIDWLVQRARTYIFTTATPPGIACAVEAALDLIASEEGDERRARLDRHIAVW
SAHAQRLAARFGWQWMPSPTAIQPIVIGENAPALALAAALEHEGIRIAAIRPPTVPAGTARLRITLSAAHTDADIERLAQ
ALEAAGQSLQPAKAA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=391, Percent_Identity=34.2710997442455, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI284448556, Length=367, Percent_Identity=35.149863760218, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI83977444, Length=407, Percent_Identity=33.9066339066339, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI83977440, Length=407, Percent_Identity=33.9066339066339, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI83977442, Length=407, Percent_Identity=33.9066339066339, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI4758668, Length=423, Percent_Identity=26.9503546099291, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4502025, Length=373, Percent_Identity=31.6353887399464, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI40316939, Length=373, Percent_Identity=31.6353887399464, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI119220554, Length=393, Percent_Identity=27.735368956743, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5454084, Length=395, Percent_Identity=24.3037974683544, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786993, Length=363, Percent_Identity=44.3526170798898, Blast_Score=258, Evalue=5e-70,
Organism=Escherichia coli, GI1790046, Length=371, Percent_Identity=37.466307277628, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI71994529, Length=379, Percent_Identity=34.0369393139842, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI32566772, Length=376, Percent_Identity=28.1914893617021, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560912, Length=420, Percent_Identity=24.7619047619048, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17560914, Length=419, Percent_Identity=24.3436754176611, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71982625, Length=291, Percent_Identity=23.0240549828179, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982617, Length=291, Percent_Identity=23.0240549828179, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320438, Length=390, Percent_Identity=32.5641025641026, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6320267, Length=375, Percent_Identity=27.4666666666667, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323954, Length=279, Percent_Identity=27.2401433691756, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24662918, Length=413, Percent_Identity=33.8983050847458, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17137420, Length=385, Percent_Identity=33.5064935064935, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI17136286, Length=365, Percent_Identity=26.8493150684932, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653280, Length=288, Percent_Identity=27.0833333333333, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653276, Length=288, Percent_Identity=27.0833333333333, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653278, Length=288, Percent_Identity=27.0833333333333, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_RALPJ (B2UBJ3)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898946.1
- ProteinModelPortal:   B2UBJ3
- GeneID:   6286984
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1372
- HOGENOM:   HBG649839
- OMA:   ATVMAMS
- ProtClustDB:   PRK05958
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 43789; Mature: 43789

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 21-21 BINDING 149-149 BINDING 195-195 BINDING 374-374

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLDDLQAGLAAIDAAHLRRVRRTAYSPTDRSQRISVPGEAPRDILGFCGNDYLGLAAH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHCCCCCCCCCCC
PALVEAMTQGTQQYGFGSGASHLVSGHSIAHARLEARMAALQGEHIPEADALFFCTGYMA
HHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHHH
NLAVVSAMAQAGGIRPAEECTIFSDALNHASLIDGARLSRAPIKVYPHVDLAALEALLAA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHH
STSRNKLIVTDGVFSMDGDIAPLPELLALAERYDAWLIVDDAHGLGVLGENGAGVLSHFG
CCCCCEEEEECCEEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEECCCCCHHHHHCC
LHSQRIIYVGTFGKAAGGSGAAIVGHRLVIDWLVQRARTYIFTTATPPGIACAVEAALDL
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHH
IASEEGDERRARLDRHIAVWSAHAQRLAARFGWQWMPSPTAIQPIVIGENAPALALAAAL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEEECCCCCHHHHHHHH
EHEGIRIAAIRPPTVPAGTARLRITLSAAHTDADIERLAQALEAAGQSLQPAKAA
HCCCEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRLLDDLQAGLAAIDAAHLRRVRRTAYSPTDRSQRISVPGEAPRDILGFCGNDYLGLAAH
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHCCCCCCCCCCC
PALVEAMTQGTQQYGFGSGASHLVSGHSIAHARLEARMAALQGEHIPEADALFFCTGYMA
HHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHHH
NLAVVSAMAQAGGIRPAEECTIFSDALNHASLIDGARLSRAPIKVYPHVDLAALEALLAA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHH
STSRNKLIVTDGVFSMDGDIAPLPELLALAERYDAWLIVDDAHGLGVLGENGAGVLSHFG
CCCCCEEEEECCEEECCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEECCCCCHHHHHCC
LHSQRIIYVGTFGKAAGGSGAAIVGHRLVIDWLVQRARTYIFTTATPPGIACAVEAALDL
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHH
IASEEGDERRARLDRHIAVWSAHAQRLAARFGWQWMPSPTAIQPIVIGENAPALALAAAL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCEEEEEECCCCCHHHHHHHH
EHEGIRIAAIRPPTVPAGTARLRITLSAAHTDADIERLAQALEAAGQSLQPAKAA
HCCCEEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA