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The map label for this gene is prpB [C]

Identifier: 187928413

GI number: 187928413

Start: 1418805

End: 1419632

Strand: Reverse

Name: prpB [C]

Synonym: Rpic_1325

Alternate gene names: 187928413

Gene position: 1419632-1418805 (Counterclockwise)

Preceding gene: 187928414

Following gene: 187928412

Centisome position: 36.01

GC content: 67.51

Gene sequence:

>828_bases
ATGCCGACCCAGATCGAAAAAGCTCGTGCCTTCCGCGTACTGCACGAACGCAGCGGCACCTTCGTGATCCCCAACCCGTG
GGACCCGGGATCGGCTCGGCTATTGGCATCACTCGGCTTCGAAGCATTGACCAGCACCAGCGCGGGCTTCGCGTTCTCGC
GCGGGCTGCCTGACTATGGCGTTGCCCGCGACACCATCCTCGCGCACGTGGCCGATCTGGCGGCCGCCACGGACGTGCCC
ATGGCTGCCGACCTGGAAAACGGCTTTGGCGATGGGCCGGAAACGGTGGCCGAGACGATCCGCCTGGCAGGCGCCACCGG
CATCGTCGGCGGCTCGATTGAAGATGCAACGGGCCGCGCAGATGCGCCCATCTATTCCCTGGAAGCTTCGGTCGACCGTG
TGCGCGCGGCGGTGGAGGCTGCGCGGTCGCTGCCGTTTCCGTTCACGCTCACCGCACGTGCCGAGAACCACCTGCACGGC
CGCAACGACCTGGCCGACACGATCCGCCGCCTGCAGGCTTTTCAGGACGCCGGCGCCGACGTGCTGTACGCCCCCGGCCT
GAAAACGCGCGACGAGATCGCCACCGTGCTGCGCGAGATCGACCGCCCGCTCAACGTGCTGATGGGCTTCGCCGACGGCA
ACCTGAGCGTGGCAGAACTGACGGAATTGGGCGTCAAGCGCATCAGCGTGGGCGCCACGCTGGCACGTGTGGCCATGGGT
GCGTTCCAGCGTGCAGCGGAAGAACTGCGCACCGCCGGGACGTTCAACTTTGCGCGCGATGCCGCCAGCGGAGCCAAGCT
CAACGGCCTCTTTGCGCCGTTCGCCTGA

Upstream 100 bases:

>100_bases
CGGCGACGCGATGTTCTGGGGCAACGACCGCCTGGACGATGCGCTCGCCTGCGCCGTAGCATTGCAGTCCAATCCTGGTT
CATATCACAGCGGATAACCC

Downstream 100 bases:

>100_bases
CGCTTGCCGCCGGGCAATCGCAACCAATCTTGGCATCCCCTCCAACCGACACCAAGCCGCCCGGCGGCCTGCGCCAGATG
CCCGCCGGCATCTGGGTGCT

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPTQIEKARAFRVLHERSGTFVIPNPWDPGSARLLASLGFEALTSTSAGFAFSRGLPDYGVARDTILAHVADLAAATDVP
MAADLENGFGDGPETVAETIRLAGATGIVGGSIEDATGRADAPIYSLEASVDRVRAAVEAARSLPFPFTLTARAENHLHG
RNDLADTIRRLQAFQDAGADVLYAPGLKTRDEIATVLREIDRPLNVLMGFADGNLSVAELTELGVKRISVGATLARVAMG
AFQRAAEELRTAGTFNFARDAASGAKLNGLFAPFA

Sequences:

>Translated_275_residues
MPTQIEKARAFRVLHERSGTFVIPNPWDPGSARLLASLGFEALTSTSAGFAFSRGLPDYGVARDTILAHVADLAAATDVP
MAADLENGFGDGPETVAETIRLAGATGIVGGSIEDATGRADAPIYSLEASVDRVRAAVEAARSLPFPFTLTARAENHLHG
RNDLADTIRRLQAFQDAGADVLYAPGLKTRDEIATVLREIDRPLNVLMGFADGNLSVAELTELGVKRISVGATLARVAMG
AFQRAAEELRTAGTFNFARDAASGAKLNGLFAPFA
>Mature_274_residues
PTQIEKARAFRVLHERSGTFVIPNPWDPGSARLLASLGFEALTSTSAGFAFSRGLPDYGVARDTILAHVADLAAATDVPM
AADLENGFGDGPETVAETIRLAGATGIVGGSIEDATGRADAPIYSLEASVDRVRAAVEAARSLPFPFTLTARAENHLHGR
NDLADTIRRLQAFQDAGADVLYAPGLKTRDEIATVLREIDRPLNVLMGFADGNLSVAELTELGVKRISVGATLARVAMGA
FQRAAEELRTAGTFNFARDAASGAKLNGLFAPFA

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=183, Percent_Identity=31.1475409836066, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 28966; Mature: 28835

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQIEKARAFRVLHERSGTFVIPNPWDPGSARLLASLGFEALTSTSAGFAFSRGLPDYG
CCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCHHHHHCCCCCCHHHCCCCCCC
VARDTILAHVADLAAATDVPMAADLENGFGDGPETVAETIRLAGATGIVGGSIEDATGRA
HHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DAPIYSLEASVDRVRAAVEAARSLPFPFTLTARAENHLHGRNDLADTIRRLQAFQDAGAD
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCC
VLYAPGLKTRDEIATVLREIDRPLNVLMGFADGNLSVAELTELGVKRISVGATLARVAMG
EEECCCCCCHHHHHHHHHHHCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFQRAAEELRTAGTFNFARDAASGAKLNGLFAPFA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTQIEKARAFRVLHERSGTFVIPNPWDPGSARLLASLGFEALTSTSAGFAFSRGLPDYG
CCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCHHHHHCCCCCCHHHCCCCCCC
VARDTILAHVADLAAATDVPMAADLENGFGDGPETVAETIRLAGATGIVGGSIEDATGRA
HHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DAPIYSLEASVDRVRAAVEAARSLPFPFTLTARAENHLHGRNDLADTIRRLQAFQDAGAD
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCC
VLYAPGLKTRDEIATVLREIDRPLNVLMGFADGNLSVAELTELGVKRISVGATLARVAMG
EEECCCCCCHHHHHHHHHHHCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFQRAAEELRTAGTFNFARDAASGAKLNGLFAPFA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]