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The map label for this gene is rluF [H]

Identifier: 187928364

GI number: 187928364

Start: 1367760

End: 1369784

Strand: Reverse

Name: rluF [H]

Synonym: Rpic_1275

Alternate gene names: 187928364

Gene position: 1369784-1367760 (Counterclockwise)

Preceding gene: 187928365

Following gene: 187928356

Centisome position: 34.74

GC content: 68.74

Gene sequence:

>2025_bases
ATGCGCTCGCCATCTGAGCGCCAGCCTTACCGGTCTAGATTCATGACTACCGATTCCGATGATTTTCCGGGCGACGACGA
CAAGCACCGCGCCAGCGACCCGACTCAGCGCGCCACCCGCCCGGGCAAGCGCGCCACGCTCGGCGTGCGCCGCGACGACG
AAGGCAATGCCGTACGCTCCACCACCGGCAAGCCGCTGCGCTCATCCGACCTGAACCGCGAGCGCCAGCCGCTGCGCCCA
TCGCAGCGCCGGCCCAAACCGGAAGAAGGCGCCGGTAAGCCGCCGCGCCCCGCGCGCCCCCCGCGCCGCGACGACGACGG
CCAGACGCGCGAAGCCAAGCCGCGCCGCATGCAAGGCGATCGCCCGCCGCGACGCTTTGATGATGAGCGCCCGCCGCGTC
GATTCAACGAAGAGCAACGACCGCCCCGCCGCTCCGACGACGAGCGTCCGCCGCGACGTTTCGAAGATCGCCCACCTCGC
CGGTTCGACGACGAGCGTCCGCCGCGCAGGTTCGACAACGACCGTCCCCCGAGACGCTTTGACGATGAGCGCCCGCCGCG
CCGGTTCAGCGACGACCAACGGCCGCCTCGTCGTTTCGATGAGGAGCGCCCACGCCGCTATGGCGACGATCAACGCGCGC
CGCGTCGTGATGGCGACCAACGCCCTCCGCGACGCTTTGACGAAGAGCGCCCGCCACGCCGGTTCAATGACGAGCAGCGG
CCACCGCGCCGCTTCGACGAAGAGCGCCCCCCGCGCAAGTTCGGTGACGATCAGCGCCCGCCCCGTCGTTTCGATGACGA
GCGCCCGCGCCGCTATGGCGACGATCAACGCGCACCGCGTCGTGATGGCGACCAACGTCCGCCGCGCCGCCTTGACGACG
AACGCCCCCCGCGCCGGTTCAGCGACGACCAACGTCCGCCCCGTCGTTTCGATGACGAACGCCCGCGCCGCTATGGCGAC
GATCAACGCGCGCCGCGTCGCGATGGCGACCAGCGGCCTCCACGACGCTTTGACGATGAACGCCCGCCGCGCCGATTCGA
CGACGAGCAACGGCCGCCGCGGCGCTATGGCGATGACCGGGCTCCGCGTCGCTTCGATGACGAACGCCCTCCGCGTCGTT
TTGACAACGACCGACCACCGCGTCGCTTCGATGATCGTCCGGCACGTCCGCAACGGCCCGAGCGCGAGCGTGAGGAACGC
GCTCCGCGTCACGCCGAGGAGCAGGCACCGCGCCCGGCGCAGCAGACCGAATCGAGCGGACTGGTCCGTCTGTCCAAGCG
CATGTCCGAACTGGGGCTGTGCTCGCGCCGGGAGGCCGATGAATGGATTCCGCGCGGCTGGGTGCTGGTGGACGGCGAGC
CCGTCACCGAACTGGGTTCGCGCGTCAAGCCGGATGCGATCATCGAGATCCATCAAGCTGCGAGATCCGAGCAAGGCGAG
CGCGTCACCGTGCTGATGCACAAGCCGGTCGGCTATGTGTCAGGCCAGGCGGAAGACGGCTATCAGCCGGCGGTTTCGCT
GTTCGTGCCGGAAAACCAGTGGGAAGCCGACCCCACGCGCAAGCGCTTCGCGCCGTGGCAGCGCAGGGCGTTGGCGCCGG
CCGGTCGACTGGATATCGACTCCACCGGCTTACTGGTGTTGACGCAGGACGGCCGTGTGGCGCGCCACCTGATCGGCGAG
GATTCGACGGTCGAGAAGGAATACCTGGTGCGCGTGGTATGGCATTCGCCGGACGGCGTGGTCGAGCGCGACATCTCCAA
GGTCTTCCCGGAAGAGCTGATGGAGAAGCTGCGCTTTGGCCTGTCGCTCGACGGCGAAGCACTCAAGCCCGCGAAGGTGA
GTTGGCAGAACGAAGAGCAGCTGCGCTTCGTGCTGCGCGAAGGCAAGAAGCGCCAGATCCGCCGCATGTGCGAACAGGTG
GGACTGGAGGTGGTGGGCCTCAAGCGCATCCGCATGGGCAGCATCGTGCTGGGCGACCTGCCGGTCGGTCAGTGGCGCTT
CCTGGGGCCGTTCGAGAAATTCTGA

Upstream 100 bases:

>100_bases
AACACGCTTGGCGAGCGCGTGGAAGACATCTTCCTGGTCGACGGCACGCGCCTGACCCAGGACAACAAACTGCAGCTCGA
ACTTGAAAGCGAGCTGCTTG

Downstream 100 bases:

>100_bases
GGCAGCCTCTGCCACAAAGAAAGAACCGGTCAACGCATTGCACGCTGACCGGTTTTTTTGTGCCCGCTGTCTACATTACA
GATGCGGCGCCTGACAAGCT

Product: RNA-binding S4 domain-containing protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MRSPSERQPYRSRFMTTDSDDFPGDDDKHRASDPTQRATRPGKRATLGVRRDDEGNAVRSTTGKPLRSSDLNRERQPLRP
SQRRPKPEEGAGKPPRPARPPRRDDDGQTREAKPRRMQGDRPPRRFDDERPPRRFNEEQRPPRRSDDERPPRRFEDRPPR
RFDDERPPRRFDNDRPPRRFDDERPPRRFSDDQRPPRRFDEERPRRYGDDQRAPRRDGDQRPPRRFDEERPPRRFNDEQR
PPRRFDEERPPRKFGDDQRPPRRFDDERPRRYGDDQRAPRRDGDQRPPRRLDDERPPRRFSDDQRPPRRFDDERPRRYGD
DQRAPRRDGDQRPPRRFDDERPPRRFDDEQRPPRRYGDDRAPRRFDDERPPRRFDNDRPPRRFDDRPARPQRPEREREER
APRHAEEQAPRPAQQTESSGLVRLSKRMSELGLCSRREADEWIPRGWVLVDGEPVTELGSRVKPDAIIEIHQAARSEQGE
RVTVLMHKPVGYVSGQAEDGYQPAVSLFVPENQWEADPTRKRFAPWQRRALAPAGRLDIDSTGLLVLTQDGRVARHLIGE
DSTVEKEYLVRVVWHSPDGVVERDISKVFPEELMEKLRFGLSLDGEALKPAKVSWQNEEQLRFVLREGKKRQIRRMCEQV
GLEVVGLKRIRMGSIVLGDLPVGQWRFLGPFEKF

Sequences:

>Translated_674_residues
MRSPSERQPYRSRFMTTDSDDFPGDDDKHRASDPTQRATRPGKRATLGVRRDDEGNAVRSTTGKPLRSSDLNRERQPLRP
SQRRPKPEEGAGKPPRPARPPRRDDDGQTREAKPRRMQGDRPPRRFDDERPPRRFNEEQRPPRRSDDERPPRRFEDRPPR
RFDDERPPRRFDNDRPPRRFDDERPPRRFSDDQRPPRRFDEERPRRYGDDQRAPRRDGDQRPPRRFDEERPPRRFNDEQR
PPRRFDEERPPRKFGDDQRPPRRFDDERPRRYGDDQRAPRRDGDQRPPRRLDDERPPRRFSDDQRPPRRFDDERPRRYGD
DQRAPRRDGDQRPPRRFDDERPPRRFDDEQRPPRRYGDDRAPRRFDDERPPRRFDNDRPPRRFDDRPARPQRPEREREER
APRHAEEQAPRPAQQTESSGLVRLSKRMSELGLCSRREADEWIPRGWVLVDGEPVTELGSRVKPDAIIEIHQAARSEQGE
RVTVLMHKPVGYVSGQAEDGYQPAVSLFVPENQWEADPTRKRFAPWQRRALAPAGRLDIDSTGLLVLTQDGRVARHLIGE
DSTVEKEYLVRVVWHSPDGVVERDISKVFPEELMEKLRFGLSLDGEALKPAKVSWQNEEQLRFVLREGKKRQIRRMCEQV
GLEVVGLKRIRMGSIVLGDLPVGQWRFLGPFEKF
>Mature_674_residues
MRSPSERQPYRSRFMTTDSDDFPGDDDKHRASDPTQRATRPGKRATLGVRRDDEGNAVRSTTGKPLRSSDLNRERQPLRP
SQRRPKPEEGAGKPPRPARPPRRDDDGQTREAKPRRMQGDRPPRRFDDERPPRRFNEEQRPPRRSDDERPPRRFEDRPPR
RFDDERPPRRFDNDRPPRRFDDERPPRRFSDDQRPPRRFDEERPRRYGDDQRAPRRDGDQRPPRRFDEERPPRRFNDEQR
PPRRFDEERPPRKFGDDQRPPRRFDDERPRRYGDDQRAPRRDGDQRPPRRLDDERPPRRFSDDQRPPRRFDDERPRRYGD
DQRAPRRDGDQRPPRRFDDERPPRRFDDEQRPPRRYGDDRAPRRFDDERPPRRFDNDRPPRRFDDRPARPQRPEREREER
APRHAEEQAPRPAQQTESSGLVRLSKRMSELGLCSRREADEWIPRGWVLVDGEPVTELGSRVKPDAIIEIHQAARSEQGE
RVTVLMHKPVGYVSGQAEDGYQPAVSLFVPENQWEADPTRKRFAPWQRRALAPAGRLDIDSTGLLVLTQDGRVARHLIGE
DSTVEKEYLVRVVWHSPDGVVERDISKVFPEELMEKLRFGLSLDGEALKPAKVSWQNEEQLRFVLREGKKRQIRRMCEQV
GLEVVGLKRIRMGSIVLGDLPVGQWRFLGPFEKF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4503509, Length=243, Percent_Identity=51.440329218107, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1790453, Length=247, Percent_Identity=34.8178137651822, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1787524, Length=254, Percent_Identity=31.1023622047244, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1788510, Length=249, Percent_Identity=31.7269076305221, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081838, Length=167, Percent_Identity=32.3353293413174, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 80357; Mature: 80357

Theoretical pI: Translated: 11.05; Mature: 11.05

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSPSERQPYRSRFMTTDSDDFPGDDDKHRASDPTQRATRPGKRATLGVRRDDEGNAVRS
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEECCCCCCCCCCCCC
TTGKPLRSSDLNRERQPLRPSQRRPKPEEGAGKPPRPARPPRRDDDGQTREAKPRRMQGD
CCCCCCHHHHCCCHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCC
RPPRRFDDERPPRRFNEEQRPPRRSDDERPPRRFEDRPPRRFDDERPPRRFDNDRPPRRF
CCCCCCCCCCCCHHCCHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
DDERPPRRFSDDQRPPRRFDEERPRRYGDDQRAPRRDGDQRPPRRFDEERPPRRFNDEQR
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
PPRRFDEERPPRKFGDDQRPPRRFDDERPRRYGDDQRAPRRDGDQRPPRRLDDERPPRRF
CCHHCCCCCCCHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SDDQRPPRRFDDERPRRYGDDQRAPRRDGDQRPPRRFDDERPPRRFDDEQRPPRRYGDDR
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCC
APRRFDDERPPRRFDNDRPPRRFDDRPARPQRPEREREERAPRHAEEQAPRPAQQTESSG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCCHHHHHHHH
LVRLSKRMSELGLCSRREADEWIPRGWVLVDGEPVTELGSRVKPDAIIEIHQAARSEQGE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCCC
RVTVLMHKPVGYVSGQAEDGYQPAVSLFVPENQWEADPTRKRFAPWQRRALAPAGRLDID
EEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCHHHHHHCCCCCCCCCC
STGLLVLTQDGRVARHLIGEDSTVEKEYLVRVVWHSPDGVVERDISKVFPEELMEKLRFG
CCCEEEEECCCHHHHHHHCCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
LSLDGEALKPAKVSWQNEEQLRFVLREGKKRQIRRMCEQVGLEVVGLKRIRMGSIVLGDL
CCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCEEEHHHHEEECCEEECCC
PVGQWRFLGPFEKF
CCCCEEECCCHHCC
>Mature Secondary Structure
MRSPSERQPYRSRFMTTDSDDFPGDDDKHRASDPTQRATRPGKRATLGVRRDDEGNAVRS
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEECCCCCCCCCCCCC
TTGKPLRSSDLNRERQPLRPSQRRPKPEEGAGKPPRPARPPRRDDDGQTREAKPRRMQGD
CCCCCCHHHHCCCHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCC
RPPRRFDDERPPRRFNEEQRPPRRSDDERPPRRFEDRPPRRFDDERPPRRFDNDRPPRRF
CCCCCCCCCCCCHHCCHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
DDERPPRRFSDDQRPPRRFDEERPRRYGDDQRAPRRDGDQRPPRRFDEERPPRRFNDEQR
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
PPRRFDEERPPRKFGDDQRPPRRFDDERPRRYGDDQRAPRRDGDQRPPRRLDDERPPRRF
CCHHCCCCCCCHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SDDQRPPRRFDDERPRRYGDDQRAPRRDGDQRPPRRFDDERPPRRFDDEQRPPRRYGDDR
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCC
APRRFDDERPPRRFDNDRPPRRFDDRPARPQRPEREREERAPRHAEEQAPRPAQQTESSG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCCHHHHHHHH
LVRLSKRMSELGLCSRREADEWIPRGWVLVDGEPVTELGSRVKPDAIIEIHQAARSEQGE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCCC
RVTVLMHKPVGYVSGQAEDGYQPAVSLFVPENQWEADPTRKRFAPWQRRALAPAGRLDID
EEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCHHHHHHCCCCCCCCCC
STGLLVLTQDGRVARHLIGEDSTVEKEYLVRVVWHSPDGVVERDISKVFPEELMEKLRFG
CCCEEEEECCCHHHHHHHCCCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
LSLDGEALKPAKVSWQNEEQLRFVLREGKKRQIRRMCEQVGLEVVGLKRIRMGSIVLGDL
CCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCEEEHHHHEEECCEEECCC
PVGQWRFLGPFEKF
CCCCEEECCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]