Click here to switch to the map view.

The map label for this gene is gsiD [H]

Identifier: 187928344

GI number: 187928344

Start: 1345575

End: 1346474

Strand: Direct

Name: gsiD [H]

Synonym: Rpic_1255

Alternate gene names: 187928344

Gene position: 1345575-1346474 (Clockwise)

Preceding gene: 187928343

Following gene: 187928345

Centisome position: 34.13

GC content: 65.0

Gene sequence:

>900_bases
ATGACCCAAGCCGCAAGCCAGGCGGTCGCACCGCAAGCCGCCACCGAACCCGTTCGCACCCCCTGGACGGAGTTCTGGCG
CAAGTTCCGCAAGCAGCATCTGGCGATGGGCGCCGGTGTGTTCGTGATCGCGCTGGTATTGATCGCCATCCTCGCGCCGC
ACATCGTGCCGTTCGATGCGGAAAACTATTTCGACTACGACGCGCTCAACGCGGGGCCGTCGGCGCAGCATTGGTTCGGC
GTCGATTCGCTGGGCCGCGACATCTTCAGCCGCATCCTGATGGGCACACGCATCTCGCTGGAAGCGGGCTTCGTGTCGGT
CATCATCGGCGCCATCATAGGCACCATGCTCGGCCTGTTGGCCGGCTACTACGAAGGCTGGTGGGACCGCATCGTCATGC
GCATCTCCGACGTGCTGTTTGCCTTCCCCGGCATCCTGCTGGCCATCGGCATCGTGGCCATCCTGGGCAACGGCATGATC
AACGTGATCTTTGCCGTGGCGGTGTTCAGCATTCCGGCATTTGCGCGGCTGGTGCGCGGCAACACGCTCATGCTCAAGCA
CCTGACGTATGTGGAAGCCGCGCGCAGCATCGGCGCATCCGACTGGACGATCATCATGCGGCACATCCTGCCGGGCACGA
TTTCATCCATCGTCGTGTACTTCTCGATGCGGATCGGCACCTCCATCATCACCGCTGCCAGCCTCTCGTTCCTGGGCCTT
GGCGCCCAGCCGCCCACGCCGGAGTGGGGCGCGATGCTCAACGAGGCGCGCGCCGACATGGTGACGGCGCCGCACGTGGC
GATCTTCCCGAGCCTGGCCATCTTCCTGACCGTGCTGGCGTTCAACCTGCTGGGCGACGGCCTGCGCGACGCGCTGGACC
CGAAGATCGACCGCCGCTGA

Upstream 100 bases:

>100_bases
CTGCTGTTCTCGCTGGAGTTCATCCTGATCAACCTTGTGGTGGACATGCTGTACACCGTCATCAACCCCACCATCCGCTA
CAAAAAGTAAGGAGGCCGCC

Downstream 100 bases:

>100_bases
GTCTGCTCGCATGTCTCTGAGTCTTCCGCACATCGGCGCACTGCCCGCGGGCGTACGCAACAGCATCGCCGACGTGGCGG
GCGTGACCGTCGGCCACGCC

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTQAASQAVAPQAATEPVRTPWTEFWRKFRKQHLAMGAGVFVIALVLIAILAPHIVPFDAENYFDYDALNAGPSAQHWFG
VDSLGRDIFSRILMGTRISLEAGFVSVIIGAIIGTMLGLLAGYYEGWWDRIVMRISDVLFAFPGILLAIGIVAILGNGMI
NVIFAVAVFSIPAFARLVRGNTLMLKHLTYVEAARSIGASDWTIIMRHILPGTISSIVVYFSMRIGTSIITAASLSFLGL
GAQPPTPEWGAMLNEARADMVTAPHVAIFPSLAIFLTVLAFNLLGDGLRDALDPKIDRR

Sequences:

>Translated_299_residues
MTQAASQAVAPQAATEPVRTPWTEFWRKFRKQHLAMGAGVFVIALVLIAILAPHIVPFDAENYFDYDALNAGPSAQHWFG
VDSLGRDIFSRILMGTRISLEAGFVSVIIGAIIGTMLGLLAGYYEGWWDRIVMRISDVLFAFPGILLAIGIVAILGNGMI
NVIFAVAVFSIPAFARLVRGNTLMLKHLTYVEAARSIGASDWTIIMRHILPGTISSIVVYFSMRIGTSIITAASLSFLGL
GAQPPTPEWGAMLNEARADMVTAPHVAIFPSLAIFLTVLAFNLLGDGLRDALDPKIDRR
>Mature_298_residues
TQAASQAVAPQAATEPVRTPWTEFWRKFRKQHLAMGAGVFVIALVLIAILAPHIVPFDAENYFDYDALNAGPSAQHWFGV
DSLGRDIFSRILMGTRISLEAGFVSVIIGAIIGTMLGLLAGYYEGWWDRIVMRISDVLFAFPGILLAIGIVAILGNGMIN
VIFAVAVFSIPAFARLVRGNTLMLKHLTYVEAARSIGASDWTIIMRHILPGTISSIVVYFSMRIGTSIITAASLSFLGLG
AQPPTPEWGAMLNEARADMVTAPHVAIFPSLAIFLTVLAFNLLGDGLRDALDPKIDRR

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=287, Percent_Identity=77.3519163763066, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1787760, Length=295, Percent_Identity=40, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1789964, Length=300, Percent_Identity=41.3333333333333, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787498, Length=282, Percent_Identity=36.5248226950355, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1789889, Length=241, Percent_Identity=39.0041493775934, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787549, Length=272, Percent_Identity=34.1911764705882, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788505, Length=225, Percent_Identity=32.8888888888889, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32443; Mature: 32311

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAASQAVAPQAATEPVRTPWTEFWRKFRKQHLAMGAGVFVIALVLIAILAPHIVPFDA
CCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
ENYFDYDALNAGPSAQHWFGVDSLGRDIFSRILMGTRISLEAGFVSVIIGAIIGTMLGLL
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AGYYEGWWDRIVMRISDVLFAFPGILLAIGIVAILGNGMINVIFAVAVFSIPAFARLVRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
NTLMLKHLTYVEAARSIGASDWTIIMRHILPGTISSIVVYFSMRIGTSIITAASLSFLGL
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAQPPTPEWGAMLNEARADMVTAPHVAIFPSLAIFLTVLAFNLLGDGLRDALDPKIDRR
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TQAASQAVAPQAATEPVRTPWTEFWRKFRKQHLAMGAGVFVIALVLIAILAPHIVPFDA
CCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCC
ENYFDYDALNAGPSAQHWFGVDSLGRDIFSRILMGTRISLEAGFVSVIIGAIIGTMLGLL
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AGYYEGWWDRIVMRISDVLFAFPGILLAIGIVAILGNGMINVIFAVAVFSIPAFARLVRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
NTLMLKHLTYVEAARSIGASDWTIIMRHILPGTISSIVVYFSMRIGTSIITAASLSFLGL
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAQPPTPEWGAMLNEARADMVTAPHVAIFPSLAIFLTVLAFNLLGDGLRDALDPKIDRR
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]