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The map label for this gene is gsiB [H]

Identifier: 187928342

GI number: 187928342

Start: 1343018

End: 1344571

Strand: Direct

Name: gsiB [H]

Synonym: Rpic_1253

Alternate gene names: 187928342

Gene position: 1343018-1344571 (Clockwise)

Preceding gene: 187928341

Following gene: 187928343

Centisome position: 34.06

GC content: 62.03

Gene sequence:

>1554_bases
ATGTTCAATCGCTTTGCTTTCAGCCCGCGCGCAGCCATGGTTGCCAGCGGCCTGGCCCTGGCGGCATTCACCATGCCGGC
CTTTGCCGCCAAGGACGCCGTGATGGCGGTCTACTCGACCTTCACCACGATGGACCCGTACGACGCCAACGACACGCTGT
CGTTCAGCGCGGCCAAGTCGTTCTATCAAGGCCTGTTCGGCTTCGACAAGGACATGAAGCTGGTCAACGTGCTGTGCGAC
AGCTACGAGGTCAGCAAGGACGGCCTCGTCTACACGTTCAAGCTGAAGAAGGGCGTGAAGTTCCACGATGGCACCACGTT
TGATGCCAACGCCGTCAAGGCCAACCTGGACCGCGTGACCGACCCGGCCAACAAGCTCAAGCGTTTCGTGCTGTTCAACC
GCGTGGCCAAGACCGAAGTTGTTGACCCGACCACCGCCCGCGTGACGCTCAAGGAGCCGTTCTCGCCGTTCATCAACGTG
CTGGCCCATCCGTCGGCGGCGATGATCTCGCCCACGGCGCTCAAGAAGTACGGCAAGGACATCGCGTTCCACCCGGTCGG
CACCGGGCCGTTCGAATTTGTCGAGTGGAAGCAGACCGACTACCTGAAGGGCAAGAAATTCGACGGCTACTGGAAACACG
GCCTGCCGAAGATCGACACCATCACCTGGAAGCCGGTGGTCGACAACAACACGCGCGCCACCATCATGCAGACGGGCGAG
GCCGACTTTGCGTTCACGATTCCGTTCGAGCAGGCTGCCGTCCTCAAGAGCAGCCCCAAGGTTGACCTGATTGCCGCGCC
GTCGATCATCCAGCGCTATGTCAGCTTCAACACCAACGTGAAGCCGTTCGACAACCCCAAGGTGCGTCAGGCGATCAACT
ATGCGATCAACAAGGATGCGCTGACCAAGGTGGCGTTCGCGGGTTACGCCGTGCCGGCCGAAGGCGTGGTGCCGCCGGGC
GTTGACTACGCGGTCAAGCTGGGCCCGTGGCCGTACAACCCGGCCAAGGCGAAAGAGCTGCTGAAGGAAGCGGGTTACCC
GAACGGCTTTGAGACGCAACTGTGGTCGGCCTATAACCACACGACGGCGCAGAAGATCATCCAGTTCGTGCAGCAGCAAC
TGGCCCAGGTCGGCATCAAGACGTCGGTGCTGGCGCTGGAAGCCGGCCAGCGCGTCGAGCGTGTGGAAAGCGTGCCCAAG
CCGGAAGACGCCGGCGTGCGCATGTATTACGCAGGCTGGTCGTCGTCGACGGGTGAGTCGGATTGGGCGCTGCGTCCGTT
GCTGGCATCGGAAGCCATGCCGCCCAAGTCGTTCAACACCGCATACTACAAGAACGAGCAGGTGGATGCCGACATCGCGG
GCGCACTGCGCACGACCGATCGCGCTGAGAAGTCGAAGCTCTACACCGACGCGCAGAAGCGCATCTGGGAAGACGCACCG
TGGGCCTTCCTGGTGACGGAGAAGATCGTCTACGCGCGCTCGAAGCGTCTGTCGGGTGCCTACGTCGCGCCGGACGGTTC
GTTCAGCTTCGACGACATCGACGTCAAGCAATAA

Upstream 100 bases:

>100_bases
GTGGCGCCAGACCACTTTGTTGCCCGCCATTCGATTGGCGGCGCGTATTAATCGCAATACAGAACGCAGTGAGACGTCAT
CTTTGATGAGGAGGAAGGAA

Downstream 100 bases:

>100_bases
GCCTCATTGCCGTACCGGCCCGCGCAGCCTTGCGCGGGCCATTGAAATGCCGCCTGCGGTGGATTTGCCATGCTGAACTA
CTTCATTAAACGTGCGCTGG

Product: family 5 extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MFNRFAFSPRAAMVASGLALAAFTMPAFAAKDAVMAVYSTFTTMDPYDANDTLSFSAAKSFYQGLFGFDKDMKLVNVLCD
SYEVSKDGLVYTFKLKKGVKFHDGTTFDANAVKANLDRVTDPANKLKRFVLFNRVAKTEVVDPTTARVTLKEPFSPFINV
LAHPSAAMISPTALKKYGKDIAFHPVGTGPFEFVEWKQTDYLKGKKFDGYWKHGLPKIDTITWKPVVDNNTRATIMQTGE
ADFAFTIPFEQAAVLKSSPKVDLIAAPSIIQRYVSFNTNVKPFDNPKVRQAINYAINKDALTKVAFAGYAVPAEGVVPPG
VDYAVKLGPWPYNPAKAKELLKEAGYPNGFETQLWSAYNHTTAQKIIQFVQQQLAQVGIKTSVLALEAGQRVERVESVPK
PEDAGVRMYYAGWSSSTGESDWALRPLLASEAMPPKSFNTAYYKNEQVDADIAGALRTTDRAEKSKLYTDAQKRIWEDAP
WAFLVTEKIVYARSKRLSGAYVAPDGSFSFDDIDVKQ

Sequences:

>Translated_517_residues
MFNRFAFSPRAAMVASGLALAAFTMPAFAAKDAVMAVYSTFTTMDPYDANDTLSFSAAKSFYQGLFGFDKDMKLVNVLCD
SYEVSKDGLVYTFKLKKGVKFHDGTTFDANAVKANLDRVTDPANKLKRFVLFNRVAKTEVVDPTTARVTLKEPFSPFINV
LAHPSAAMISPTALKKYGKDIAFHPVGTGPFEFVEWKQTDYLKGKKFDGYWKHGLPKIDTITWKPVVDNNTRATIMQTGE
ADFAFTIPFEQAAVLKSSPKVDLIAAPSIIQRYVSFNTNVKPFDNPKVRQAINYAINKDALTKVAFAGYAVPAEGVVPPG
VDYAVKLGPWPYNPAKAKELLKEAGYPNGFETQLWSAYNHTTAQKIIQFVQQQLAQVGIKTSVLALEAGQRVERVESVPK
PEDAGVRMYYAGWSSSTGESDWALRPLLASEAMPPKSFNTAYYKNEQVDADIAGALRTTDRAEKSKLYTDAQKRIWEDAP
WAFLVTEKIVYARSKRLSGAYVAPDGSFSFDDIDVKQ
>Mature_517_residues
MFNRFAFSPRAAMVASGLALAAFTMPAFAAKDAVMAVYSTFTTMDPYDANDTLSFSAAKSFYQGLFGFDKDMKLVNVLCD
SYEVSKDGLVYTFKLKKGVKFHDGTTFDANAVKANLDRVTDPANKLKRFVLFNRVAKTEVVDPTTARVTLKEPFSPFINV
LAHPSAAMISPTALKKYGKDIAFHPVGTGPFEFVEWKQTDYLKGKKFDGYWKHGLPKIDTITWKPVVDNNTRATIMQTGE
ADFAFTIPFEQAAVLKSSPKVDLIAAPSIIQRYVSFNTNVKPFDNPKVRQAINYAINKDALTKVAFAGYAVPAEGVVPPG
VDYAVKLGPWPYNPAKAKELLKEAGYPNGFETQLWSAYNHTTAQKIIQFVQQQLAQVGIKTSVLALEAGQRVERVESVPK
PEDAGVRMYYAGWSSSTGESDWALRPLLASEAMPPKSFNTAYYKNEQVDADIAGALRTTDRAEKSKLYTDAQKRIWEDAP
WAFLVTEKIVYARSKRLSGAYVAPDGSFSFDDIDVKQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=507, Percent_Identity=63.3136094674556, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=549, Percent_Identity=27.8688524590164, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1787551, Length=534, Percent_Identity=26.4044943820225, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1789887, Length=493, Percent_Identity=26.369168356998, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1787762, Length=490, Percent_Identity=26.1224489795918, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1787495, Length=528, Percent_Identity=24.8106060606061, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789397, Length=465, Percent_Identity=21.0752688172043, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57284; Mature: 57284

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNRFAFSPRAAMVASGLALAAFTMPAFAAKDAVMAVYSTFTTMDPYDANDTLSFSAAKS
CCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
FYQGLFGFDKDMKLVNVLCDSYEVSKDGLVYTFKLKKGVKFHDGTTFDANAVKANLDRVT
HHHHHHCCCCCHHHHHHHHCCCEECCCCEEEEEEECCCCEECCCCCCCCHHHHHHHHHCC
DPANKLKRFVLFNRVAKTEVVDPTTARVTLKEPFSPFINVLAHPSAAMISPTALKKYGKD
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCHHHCCHHHHHHHCCC
IAFHPVGTGPFEFVEWKQTDYLKGKKFDGYWKHGLPKIDTITWKPVVDNNTRATIMQTGE
EEEECCCCCCHHHEECCCCCCCCCCCCCCHHHCCCCCCCEEEEEEEECCCCEEEEEECCC
ADFAFTIPFEQAAVLKSSPKVDLIAAPSIIQRYVSFNTNVKPFDNPKVRQAINYAINKDA
CCEEEECCCHHHHHHCCCCCEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHH
LTKVAFAGYAVPAEGVVPPGVDYAVKLGPWPYNPAKAKELLKEAGYPNGFETQLWSAYNH
HHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCH
TTAQKIIQFVQQQLAQVGIKTSVLALEAGQRVERVESVPKPEDAGVRMYYAGWSSSTGES
HHHHHHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCC
DWALRPLLASEAMPPKSFNTAYYKNEQVDADIAGALRTTDRAEKSKLYTDAQKRIWEDAP
CCHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
WAFLVTEKIVYARSKRLSGAYVAPDGSFSFDDIDVKQ
EEEEHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MFNRFAFSPRAAMVASGLALAAFTMPAFAAKDAVMAVYSTFTTMDPYDANDTLSFSAAKS
CCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
FYQGLFGFDKDMKLVNVLCDSYEVSKDGLVYTFKLKKGVKFHDGTTFDANAVKANLDRVT
HHHHHHCCCCCHHHHHHHHCCCEECCCCEEEEEEECCCCEECCCCCCCCHHHHHHHHHCC
DPANKLKRFVLFNRVAKTEVVDPTTARVTLKEPFSPFINVLAHPSAAMISPTALKKYGKD
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCHHHCCHHHHHHHCCC
IAFHPVGTGPFEFVEWKQTDYLKGKKFDGYWKHGLPKIDTITWKPVVDNNTRATIMQTGE
EEEECCCCCCHHHEECCCCCCCCCCCCCCHHHCCCCCCCEEEEEEEECCCCEEEEEECCC
ADFAFTIPFEQAAVLKSSPKVDLIAAPSIIQRYVSFNTNVKPFDNPKVRQAINYAINKDA
CCEEEECCCHHHHHHCCCCCEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHH
LTKVAFAGYAVPAEGVVPPGVDYAVKLGPWPYNPAKAKELLKEAGYPNGFETQLWSAYNH
HHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCH
TTAQKIIQFVQQQLAQVGIKTSVLALEAGQRVERVESVPKPEDAGVRMYYAGWSSSTGES
HHHHHHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCC
DWALRPLLASEAMPPKSFNTAYYKNEQVDADIAGALRTTDRAEKSKLYTDAQKRIWEDAP
CCHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
WAFLVTEKIVYARSKRLSGAYVAPDGSFSFDDIDVKQ
EEEEHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA