Click here to switch to the map view.

The map label for this gene is ansB [H]

Identifier: 187928270

GI number: 187928270

Start: 1269882

End: 1270874

Strand: Direct

Name: ansB [H]

Synonym: Rpic_1181

Alternate gene names: 187928270

Gene position: 1269882-1270874 (Clockwise)

Preceding gene: 187928269

Following gene: 187928271

Centisome position: 32.21

GC content: 65.06

Gene sequence:

>993_bases
ATGTCCTTGCCTACCATCGCCATCCTCGCCACCGGCGGCACGATCGCCGGCTCTGCTGACGACGCTGGCTCCGCCGCACG
CTATCGCGCTGGCGCCGTCCCCATCGATCAACTGCTCGCGGCCAGCAAGCTTGGTTTGGAGCGCCTCGCCAACGTGCGCG
CTGAACAGGTCGCACAGATCGATAGCAAGGATCTGACCTTCGACGTATGGGAAAAGCTGGTGGCGCGTATCCGTCACTGG
ATCGACGTCGAGCGTGTGGATGGCGTGGTCATCACGCACGGCACTGACACGCTGGAAGAGACCGCGATGCTACTGCACCT
GACGCAGCAGACCGATACACCGATCGTCCTGACTGCGGCGATGCGGCCGTCGACGTCGCTGTCGGCGGATGGCCCGCTGA
ATCTGCTGAATGCGGTGCGGCTGGCGGCCAGTCCGTCTGCGCGCGGGCGAGGCGTGCTGGTGGCGCTCAACCAGCGCGTG
CATGCCGCCCGCGATGTCCAGAAGGGTCACACTTACGCGGTTGAGGCGTTTGTTTCGCCGGACACCGGCGCGCTGGGTTT
CGTGCTCGACACGCAGGTGCAGTTTCAGCGGGTAGGGCAACGTGTGGCCGCAGCCGACGTGCTGCCGATGCCGCCTGCGG
GCCAGTGGCCCTGGGTTGAGGTTGTGGCGAGCTATGCCCAGCCCGATGCTCGCGTGGTGGATGCGCTGGTTGCCGCGGGC
GTGAAGGGGCTGGTGATTGCCGCAACCGGTGCGGGTTCCATCCATGCCAATCTTGAAGCGGCGCTGAACCGGGCGAGTCA
GCAGGGGGTTTTCGTACTGCGCTCCACGCGTACCGGTGCTGGCGTGGTGCCTGCGCTGCCTAGTGCGGACCGCTGGGCGT
CGAGCGGTACGCTCAACCCATATAAGGCGCGTGTGCTTCTGACGTTGCTTTTAGCCTCTGGTCGCGCCCAGGCAGAAGTT
GCCTCGCTTCAGCAAGTCATTGATCGATATTGA

Upstream 100 bases:

>100_bases
CGGCGTTTTGTTTTTGCCGGCGCCCCGGGCAATCCCGCGTCGGCAAAGGCGATGCGGGCGGATACAATGCGCGCCTTGCC
CACCACGCATGTCTGCAACG

Downstream 100 bases:

>100_bases
AAAAGTCGCAAAAGCCTGCGTATAATAGCAGGCTAACCAACCTTGCTGCACCGGAGTGTGACGCCCGGGCGGCTTAATCC
ACAAGGAGCGTCAATGCGTC

Product: asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSLPTIAILATGGTIAGSADDAGSAARYRAGAVPIDQLLAASKLGLERLANVRAEQVAQIDSKDLTFDVWEKLVARIRHW
IDVERVDGVVITHGTDTLEETAMLLHLTQQTDTPIVLTAAMRPSTSLSADGPLNLLNAVRLAASPSARGRGVLVALNQRV
HAARDVQKGHTYAVEAFVSPDTGALGFVLDTQVQFQRVGQRVAAADVLPMPPAGQWPWVEVVASYAQPDARVVDALVAAG
VKGLVIAATGAGSIHANLEAALNRASQQGVFVLRSTRTGAGVVPALPSADRWASSGTLNPYKARVLLTLLLASGRAQAEV
ASLQQVIDRY

Sequences:

>Translated_330_residues
MSLPTIAILATGGTIAGSADDAGSAARYRAGAVPIDQLLAASKLGLERLANVRAEQVAQIDSKDLTFDVWEKLVARIRHW
IDVERVDGVVITHGTDTLEETAMLLHLTQQTDTPIVLTAAMRPSTSLSADGPLNLLNAVRLAASPSARGRGVLVALNQRV
HAARDVQKGHTYAVEAFVSPDTGALGFVLDTQVQFQRVGQRVAAADVLPMPPAGQWPWVEVVASYAQPDARVVDALVAAG
VKGLVIAATGAGSIHANLEAALNRASQQGVFVLRSTRTGAGVVPALPSADRWASSGTLNPYKARVLLTLLLASGRAQAEV
ASLQQVIDRY
>Mature_329_residues
SLPTIAILATGGTIAGSADDAGSAARYRAGAVPIDQLLAASKLGLERLANVRAEQVAQIDSKDLTFDVWEKLVARIRHWI
DVERVDGVVITHGTDTLEETAMLLHLTQQTDTPIVLTAAMRPSTSLSADGPLNLLNAVRLAASPSARGRGVLVALNQRVH
AARDVQKGHTYAVEAFVSPDTGALGFVLDTQVQFQRVGQRVAAADVLPMPPAGQWPWVEVVASYAQPDARVVDALVAAGV
KGLVIAATGAGSIHANLEAALNRASQQGVFVLRSTRTGAGVVPALPSADRWASSGTLNPYKARVLLTLLLASGRAQAEVA
SLQQVIDRY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=335, Percent_Identity=40.8955223880597, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788065, Length=298, Percent_Identity=27.5167785234899, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320527, Length=330, Percent_Identity=35.1515151515151, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323189, Length=327, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6323187, Length=327, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6323186, Length=327, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6323184, Length=327, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 34663; Mature: 34532

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPTIAILATGGTIAGSADDAGSAARYRAGAVPIDQLLAASKLGLERLANVRAEQVAQI
CCCCEEEEEEECCEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DSKDLTFDVWEKLVARIRHWIDVERVDGVVITHGTDTLEETAMLLHLTQQTDTPIVLTAA
CCCCCCHHHHHHHHHHHHHHHCHHHHCCEEEECCCCHHHHHHHHHEEECCCCCCEEEEEE
MRPSTSLSADGPLNLLNAVRLAASPSARGRGVLVALNQRVHAARDVQKGHTYAVEAFVSP
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCEEEEEEEECC
DTGALGFVLDTQVQFQRVGQRVAAADVLPMPPAGQWPWVEVVASYAQPDARVVDALVAAG
CCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCC
VKGLVIAATGAGSIHANLEAALNRASQQGVFVLRSTRTGAGVVPALPSADRWASSGTLNP
CCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
YKARVLLTLLLASGRAQAEVASLQQVIDRY
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLPTIAILATGGTIAGSADDAGSAARYRAGAVPIDQLLAASKLGLERLANVRAEQVAQI
CCCEEEEEEECCEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DSKDLTFDVWEKLVARIRHWIDVERVDGVVITHGTDTLEETAMLLHLTQQTDTPIVLTAA
CCCCCCHHHHHHHHHHHHHHHCHHHHCCEEEECCCCHHHHHHHHHEEECCCCCCEEEEEE
MRPSTSLSADGPLNLLNAVRLAASPSARGRGVLVALNQRVHAARDVQKGHTYAVEAFVSP
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCCEEEEEEEECC
DTGALGFVLDTQVQFQRVGQRVAAADVLPMPPAGQWPWVEVVASYAQPDARVVDALVAAG
CCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCC
VKGLVIAATGAGSIHANLEAALNRASQQGVFVLRSTRTGAGVVPALPSADRWASSGTLNP
CCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
YKARVLLTLLLASGRAQAEVASLQQVIDRY
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026924; 3194219; 3379033; 8348975 [H]