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The map label for this gene is ycgF [H]

Identifier: 187928261

GI number: 187928261

Start: 1261874

End: 1262650

Strand: Reverse

Name: ycgF [H]

Synonym: Rpic_1172

Alternate gene names: 187928261

Gene position: 1262650-1261874 (Counterclockwise)

Preceding gene: 187928262

Following gene: 187928260

Centisome position: 32.03

GC content: 56.63

Gene sequence:

>777_bases
ATGATTCAGATCCATAACGCAAGCCAACCATCGGCACCCAACAAGCGCTGCTCCGGATGCAACTCCGATGATGGCACCGC
TATCGAGATGGCCTTCGCATTTCAGCCGATCGTTGATCTGCGCACGGGCGACGCCTACGCACACGAAGCGCTCGTACGCG
GACCCAACGGAGAGCCTGCGGCATCGGTGCTGACGCAAGTGGACGAGGCAAACCGTTACCGGTTTGACCAACGGTGCCGC
ACCACCGCAATTGAGCAGGCGGCCGGGATAGGGATGGACACCTTTCTATCCATCAATTTCATGCCGAATGCGGTGTATCA
GCCTGCCGCATGTATCCGCACAACGTTTGAAGCGGCCGAAAAATTCGGCTTTCCCATCAATCGGATCATCTTCGAAACCA
TCGAGGGCGAAGACATCATCAACCGCCCCCACCTCCTGGAGATCTTTCGGGCATATCAATCGTTCGGCTTCCAAACCGCG
ATTGATGATTTCGGTGCCGGCCACTCCGGGCTCACCCTACTCGCCGATTTCCAGCCGGACCTGATCAAGTTGGATATGGC
ACTTATCCGAGGAATCGATTCAGATCTGGTGCGTCAACGCATTGTTTGCGGTGTGCTCAGCATCTGCAACGACCTCGGGA
TCCGGGTCATTGCCGAAGGTGTTGAAACGGCACCGGAACGCGACTTCCTCGCTGCCGCGGGTATCACGCTCATGCAAGGC
TATCTGTTCGCAAAGCCTACGTTCAGAGCAATGCCACCGGTATCAAACACTCGCTGA

Upstream 100 bases:

>100_bases
CGTCGCACAAGCCTGCTTGGCAGGAGCGTGCAGTGTTCAACGACCACGCCCCGACCAGGCGTGAAGCCGTTGCCCAAAAT
CCTTTACTCGTTTTGCCAAG

Downstream 100 bases:

>100_bases
TGGTCACACGGACTTTAGCCAGACCGACTGCGGGCACACCGGCTATCCAGCTTCGGGCCGATCCGTCCGGGCTGGCGGAC
GGCTGATTCCCATCAACCCC

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MIQIHNASQPSAPNKRCSGCNSDDGTAIEMAFAFQPIVDLRTGDAYAHEALVRGPNGEPAASVLTQVDEANRYRFDQRCR
TTAIEQAAGIGMDTFLSINFMPNAVYQPAACIRTTFEAAEKFGFPINRIIFETIEGEDIINRPHLLEIFRAYQSFGFQTA
IDDFGAGHSGLTLLADFQPDLIKLDMALIRGIDSDLVRQRIVCGVLSICNDLGIRVIAEGVETAPERDFLAAAGITLMQG
YLFAKPTFRAMPPVSNTR

Sequences:

>Translated_258_residues
MIQIHNASQPSAPNKRCSGCNSDDGTAIEMAFAFQPIVDLRTGDAYAHEALVRGPNGEPAASVLTQVDEANRYRFDQRCR
TTAIEQAAGIGMDTFLSINFMPNAVYQPAACIRTTFEAAEKFGFPINRIIFETIEGEDIINRPHLLEIFRAYQSFGFQTA
IDDFGAGHSGLTLLADFQPDLIKLDMALIRGIDSDLVRQRIVCGVLSICNDLGIRVIAEGVETAPERDFLAAAGITLMQG
YLFAKPTFRAMPPVSNTR
>Mature_258_residues
MIQIHNASQPSAPNKRCSGCNSDDGTAIEMAFAFQPIVDLRTGDAYAHEALVRGPNGEPAASVLTQVDEANRYRFDQRCR
TTAIEQAAGIGMDTFLSINFMPNAVYQPAACIRTTFEAAEKFGFPINRIIFETIEGEDIINRPHLLEIFRAYQSFGFQTA
IDDFGAGHSGLTLLADFQPDLIKLDMALIRGIDSDLVRQRIVCGVLSICNDLGIRVIAEGVETAPERDFLAAAGITLMQG
YLFAKPTFRAMPPVSNTR

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=225, Percent_Identity=32.4444444444444, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI226510982, Length=262, Percent_Identity=28.2442748091603, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1790496, Length=235, Percent_Identity=26.3829787234043, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787541, Length=240, Percent_Identity=30.4166666666667, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788502, Length=227, Percent_Identity=27.3127753303965, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081845, Length=228, Percent_Identity=28.9473684210526, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788849, Length=222, Percent_Identity=30.1801801801802, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081980, Length=122, Percent_Identity=32.7868852459016, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081921, Length=243, Percent_Identity=26.7489711934156, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786507, Length=127, Percent_Identity=29.1338582677165, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082096, Length=150, Percent_Identity=28, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081743, Length=98, Percent_Identity=27.5510204081633, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 28268; Mature: 28268

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIHNASQPSAPNKRCSGCNSDDGTAIEMAFAFQPIVDLRTGDAYAHEALVRGPNGEPA
CEEECCCCCCCCCCHHCCCCCCCCCCEEEEEEHHCCHHHCCCCCHHHHHHHHCCCCCCHH
ASVLTQVDEANRYRFDQRCRTTAIEQAAGIGMDTFLSINFMPNAVYQPAACIRTTFEAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHCCHHHHHHHHHHHH
KFGFPINRIIFETIEGEDIINRPHLLEIFRAYQSFGFQTAIDDFGAGHSGLTLLADFQPD
HHCCCHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCCH
LIKLDMALIRGIDSDLVRQRIVCGVLSICNDLGIRVIAEGVETAPERDFLAAAGITLMQG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
YLFAKPTFRAMPPVSNTR
HHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIQIHNASQPSAPNKRCSGCNSDDGTAIEMAFAFQPIVDLRTGDAYAHEALVRGPNGEPA
CEEECCCCCCCCCCHHCCCCCCCCCCEEEEEEHHCCHHHCCCCCHHHHHHHHCCCCCCHH
ASVLTQVDEANRYRFDQRCRTTAIEQAAGIGMDTFLSINFMPNAVYQPAACIRTTFEAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHCCHHHHHHHHHHHH
KFGFPINRIIFETIEGEDIINRPHLLEIFRAYQSFGFQTAIDDFGAGHSGLTLLADFQPD
HHCCCHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCCH
LIKLDMALIRGIDSDLVRQRIVCGVLSICNDLGIRVIAEGVETAPERDFLAAAGITLMQG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
YLFAKPTFRAMPPVSNTR
HHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]