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The map label for this gene is galE [H]

Identifier: 187928247

GI number: 187928247

Start: 1239167

End: 1240060

Strand: Reverse

Name: galE [H]

Synonym: Rpic_1157

Alternate gene names: 187928247

Gene position: 1240060-1239167 (Counterclockwise)

Preceding gene: 187928248

Following gene: 187928246

Centisome position: 31.45

GC content: 61.07

Gene sequence:

>894_bases
ATGGCAAAGGTCCTGATTACCGGCATTCGCGGCTTCACCGGGCAGCACTTGCTCAAGGAGTTGCTTGATGCCGGTCACGA
AGTCTGCGGCCTGGCACACACACCGGCGCCCGAGGCGTCGGTGCGTGTGCATGTGTGCGACCTGCTTGACCGCACGGCAC
TGACGGAGGTGCTCGCCCGAGAGCAGCCCGAGGCGGTGGTGCACCTCGCCGCCATTGCGTTTGTTGCCCATGGCGACGCC
CGGGCAATCTACGAGACCAATGTGGTGGGCACCCGCAATCTGCTGGATGCTATCCATACGTCGGGCTGTCGGCCAAAGTC
CGTACTGCTGGCCAGCAGCGCCAACATCTACGGCAATGCAGACCGCGAGGTGATTGACGAGTCCACTCCCGCTGCGCCTG
CCAATGACTACGCGGTCAGCAAGCTGGCCATGGAATACATGGCCAGGCTGTGGGAAGACAAGCTGCCAATCACCATCGTG
AGGCCGTTCAACTACACAGGGGTAGGACAGTCGCCCAACTTCCTGCTGCCGAAGATCGTCTCGCACTTCCGCGAGCGCAA
GCCTGTCCTAGAACTGGGGAATCTGCACGTCGTCCGCGATTTCTCCGATGTGCGCAGCGTTGTGTCCGCCTATGGGCGCT
TGCTCGACGAGCAATTCGCGGGCCAGACCTTCAATGTCTGTTCGGGTACTGGCTATGCGTTGGAGGATGTGCTGGCCATG
ATGTGCGATCTCGCCGGATACCAGCCGGAGATCAAGGTGAACATGGCCTTTGTACGCGCCAACGAAGTGCACACGCTGAT
CGGTAGCAACGCCAAGTTGCGTGAGGCCATTGGCGCGCAGCCCACCATTGCGTTGCGCGACACACTGCAGTGGATGCTGG
AAGCCGAAGAATGA

Upstream 100 bases:

>100_bases
AGAACTGGGCTGGGAGCCCAAGACGACGCTTGAGCAACTGTGCCAGATGATGGTCGAGGCAGACGTCCGGCGCAATCAGG
CAGGCTTCTCGTTCTAAGAC

Downstream 100 bases:

>100_bases
AGCTGATCCTTGCGGTGGATGCGATCGTGCCACCATTGACGGGCATCGGCCGCTACACGTGGGAACTGGCCAGGCACTAC
ACCCTGCCGCAAATCGGACT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAKVLITGIRGFTGQHLLKELLDAGHEVCGLAHTPAPEASVRVHVCDLLDRTALTEVLAREQPEAVVHLAAIAFVAHGDA
RAIYETNVVGTRNLLDAIHTSGCRPKSVLLASSANIYGNADREVIDESTPAAPANDYAVSKLAMEYMARLWEDKLPITIV
RPFNYTGVGQSPNFLLPKIVSHFRERKPVLELGNLHVVRDFSDVRSVVSAYGRLLDEQFAGQTFNVCSGTGYALEDVLAM
MCDLAGYQPEIKVNMAFVRANEVHTLIGSNAKLREAIGAQPTIALRDTLQWMLEAEE

Sequences:

>Translated_297_residues
MAKVLITGIRGFTGQHLLKELLDAGHEVCGLAHTPAPEASVRVHVCDLLDRTALTEVLAREQPEAVVHLAAIAFVAHGDA
RAIYETNVVGTRNLLDAIHTSGCRPKSVLLASSANIYGNADREVIDESTPAAPANDYAVSKLAMEYMARLWEDKLPITIV
RPFNYTGVGQSPNFLLPKIVSHFRERKPVLELGNLHVVRDFSDVRSVVSAYGRLLDEQFAGQTFNVCSGTGYALEDVLAM
MCDLAGYQPEIKVNMAFVRANEVHTLIGSNAKLREAIGAQPTIALRDTLQWMLEAEE
>Mature_296_residues
AKVLITGIRGFTGQHLLKELLDAGHEVCGLAHTPAPEASVRVHVCDLLDRTALTEVLAREQPEAVVHLAAIAFVAHGDAR
AIYETNVVGTRNLLDAIHTSGCRPKSVLLASSANIYGNADREVIDESTPAAPANDYAVSKLAMEYMARLWEDKLPITIVR
PFNYTGVGQSPNFLLPKIVSHFRERKPVLELGNLHVVRDFSDVRSVVSAYGRLLDEQFAGQTFNVCSGTGYALEDVLAMM
CDLAGYQPEIKVNMAFVRANEVHTLIGSNAKLREAIGAQPTIALRDTLQWMLEAEE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=295, Percent_Identity=29.4915254237288, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI56118217, Length=295, Percent_Identity=29.4915254237288, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI189083684, Length=295, Percent_Identity=29.4915254237288, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI7657641, Length=249, Percent_Identity=28.1124497991968, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4504031, Length=337, Percent_Identity=24.6290801186944, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788366, Length=348, Percent_Identity=27.0114942528736, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788353, Length=335, Percent_Identity=24.1791044776119, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI48994969, Length=338, Percent_Identity=26.6272189349112, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786974, Length=282, Percent_Identity=26.241134751773, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=341, Percent_Identity=26.099706744868, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI133901786, Length=337, Percent_Identity=24.0356083086053, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI133901788, Length=337, Percent_Identity=24.0356083086053, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17539422, Length=337, Percent_Identity=24.3323442136499, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI133901790, Length=337, Percent_Identity=24.3323442136499, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17539424, Length=337, Percent_Identity=24.0356083086053, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=256, Percent_Identity=25.78125, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=348, Percent_Identity=24.4252873563218, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24158427, Length=338, Percent_Identity=22.7810650887574, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR005886
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 32460; Mature: 32328

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVLITGIRGFTGQHLLKELLDAGHEVCGLAHTPAPEASVRVHVCDLLDRTALTEVLAR
CCCEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHC
EQPEAVVHLAAIAFVAHGDARAIYETNVVGTRNLLDAIHTSGCRPKSVLLASSANIYGNA
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCHHEEEEECCCCCCCC
DREVIDESTPAAPANDYAVSKLAMEYMARLWEDKLPITIVRPFNYTGVGQSPNFLLPKIV
CHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHH
SHFRERKPVLELGNLHVVRDFSDVRSVVSAYGRLLDEQFAGQTFNVCSGTGYALEDVLAM
HHHHHCCCHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHH
MCDLAGYQPEIKVNMAFVRANEVHTLIGSNAKLREAIGAQPTIALRDTLQWMLEAEE
HHHHCCCCCCEEEEEEEEECCHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKVLITGIRGFTGQHLLKELLDAGHEVCGLAHTPAPEASVRVHVCDLLDRTALTEVLAR
CCEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHC
EQPEAVVHLAAIAFVAHGDARAIYETNVVGTRNLLDAIHTSGCRPKSVLLASSANIYGNA
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCHHEEEEECCCCCCCC
DREVIDESTPAAPANDYAVSKLAMEYMARLWEDKLPITIVRPFNYTGVGQSPNFLLPKIV
CHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHH
SHFRERKPVLELGNLHVVRDFSDVRSVVSAYGRLLDEQFAGQTFNVCSGTGYALEDVLAM
HHHHHCCCHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHH
MCDLAGYQPEIKVNMAFVRANEVHTLIGSNAKLREAIGAQPTIALRDTLQWMLEAEE
HHHHCCCCCCEEEEEEEEECCHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9603808 [H]