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The map label for this gene is gca [H]

Identifier: 187928248

GI number: 187928248

Start: 1240064

End: 1241098

Strand: Reverse

Name: gca [H]

Synonym: Rpic_1158

Alternate gene names: 187928248

Gene position: 1241098-1240064 (Counterclockwise)

Preceding gene: 187928249

Following gene: 187928247

Centisome position: 31.48

GC content: 58.74

Gene sequence:

>1035_bases
ATGACCACTGCTATCATCACCGGCATCACTGGCCAAGACGGCGCCTACCTAGCTCAACTGCTCTTGGAAAAAGGTTACAC
CGTCTACGGCACGTACCGCCGCACCAGTTCCGTCAATTTCTGGCGAGTCGAGGAACTCGGCGTAGATAAGCACCCGAACC
TACATCTGGTCGAGTACGACCTGACCGATCTGAGCGCCAGCATCCGCCTCCTACAGACGACCAAGGCCACCGAAGTCTAT
AACCTCGCCGCGCAAAGTTTCGTCGGCGTATCGTTCGACCAGCCCGCCACCACGGCCGAGATCACCGGCATCGGCCCGCT
CAACCTGCTCGAAGCAATCCGCATCGTCAACCCGGAGATTCGCTTCTACCAAGCCAGCACCTCCGAGATGTTCGGTAAGG
TCCAAGCCATTCCCCAGATCGAGAGCACGCCGTTCTATCCGCGCAGCCCTTACGGCGTGGCCAAGCTGTACGCACACTGG
ATTACCGTGAACTACCGCGAGAGCTACAACATCTTCGGCAGCAGCGGCATTCTGTTCAATCACGAGTCGCCGCTGCGTGG
CCGTGAGTTCGTGACCCGCAAGATTACCGACAGCCTAGCCAAGATCCATCTGGGCAAGCTGGACGTGCTGGAACTGGGCA
ACCTCGACGCCAAGCGCGACTGGGGTTTTGCCAAGGAGTACGTTGAGGGTATGTGGCGCATGCTGCAGGCTGACAAGCCA
GATACCTACGTGCTGGCCACCAACCGTACAGAAACGGTCCGTGACTTCGTCACGATGGCGGCCAAGGCGGTCGATACCGA
AATCATGTGGGAAGGCCATGCCGAGAGCGAAGTCGGCTACGATCGCAAAACAAACAAAGCCATCGTCAAGATCAATCCCA
AGTTCTACCGCCCGGCTGAAGTCGAGCTGCTGATCGGCAATCCCGCCAAGGCCCGCGAAGAACTGGGCTGGGAGCCCAAG
ACGACGCTTGAGCAACTGTGCCAGATGATGGTCGAGGCAGACGTCCGGCGCAATCAGGCAGGCTTCTCGTTCTAA

Upstream 100 bases:

>100_bases
GCGCCCTTCTGCATCAGCAGCCGGAAAACTAGGCTGCCACGAAGGAAATAGCCCACCAACCCTATTTAATTTATTTCACT
TTAAAATCAGAGAAATAAGA

Downstream 100 bases:

>100_bases
GACATGGCAAAGGTCCTGATTACCGGCATTCGCGGCTTCACCGGGCAGCACTTGCTCAAGGAGTTGCTTGATGCCGGTCA
CGAAGTCTGCGGCCTGGCAC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTTAIITGITGQDGAYLAQLLLEKGYTVYGTYRRTSSVNFWRVEELGVDKHPNLHLVEYDLTDLSASIRLLQTTKATEVY
NLAAQSFVGVSFDQPATTAEITGIGPLNLLEAIRIVNPEIRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLYAHW
ITVNYRESYNIFGSSGILFNHESPLRGREFVTRKITDSLAKIHLGKLDVLELGNLDAKRDWGFAKEYVEGMWRMLQADKP
DTYVLATNRTETVRDFVTMAAKAVDTEIMWEGHAESEVGYDRKTNKAIVKINPKFYRPAEVELLIGNPAKAREELGWEPK
TTLEQLCQMMVEADVRRNQAGFSF

Sequences:

>Translated_344_residues
MTTAIITGITGQDGAYLAQLLLEKGYTVYGTYRRTSSVNFWRVEELGVDKHPNLHLVEYDLTDLSASIRLLQTTKATEVY
NLAAQSFVGVSFDQPATTAEITGIGPLNLLEAIRIVNPEIRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLYAHW
ITVNYRESYNIFGSSGILFNHESPLRGREFVTRKITDSLAKIHLGKLDVLELGNLDAKRDWGFAKEYVEGMWRMLQADKP
DTYVLATNRTETVRDFVTMAAKAVDTEIMWEGHAESEVGYDRKTNKAIVKINPKFYRPAEVELLIGNPAKAREELGWEPK
TTLEQLCQMMVEADVRRNQAGFSF
>Mature_343_residues
TTAIITGITGQDGAYLAQLLLEKGYTVYGTYRRTSSVNFWRVEELGVDKHPNLHLVEYDLTDLSASIRLLQTTKATEVYN
LAAQSFVGVSFDQPATTAEITGIGPLNLLEAIRIVNPEIRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLYAHWI
TVNYRESYNIFGSSGILFNHESPLRGREFVTRKITDSLAKIHLGKLDVLELGNLDAKRDWGFAKEYVEGMWRMLQADKPD
TYVLATNRTETVRDFVTMAAKAVDTEIMWEGHAESEVGYDRKTNKAIVKINPKFYRPAEVELLIGNPAKAREELGWEPKT
TLEQLCQMMVEADVRRNQAGFSF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=53.3527696793003, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1788366, Length=347, Percent_Identity=51.8731988472622, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI17507723, Length=339, Percent_Identity=55.4572271386431, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI133901788, Length=339, Percent_Identity=55.1622418879056, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17539424, Length=339, Percent_Identity=55.1622418879056, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI133901790, Length=339, Percent_Identity=55.1622418879056, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17539422, Length=339, Percent_Identity=55.1622418879056, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI133901786, Length=339, Percent_Identity=55.1622418879056, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24158427, Length=338, Percent_Identity=53.2544378698225, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38885; Mature: 38753

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAIITGITGQDGAYLAQLLLEKGYTVYGTYRRTSSVNFWRVEELGVDKHPNLHLVEYD
CCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEHCCCCCCCCCEEEEEEE
LTDLSASIRLLQTTKATEVYNLAAQSFVGVSFDQPATTAEITGIGPLNLLEAIRIVNPEI
CHHHHHHEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCE
RFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLYAHWITVNYRESYNIFGSSGILFN
EEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEEEEECCCEEEECCCEEEC
HESPLRGREFVTRKITDSLAKIHLGKLDVLELGNLDAKRDWGFAKEYVEGMWRMLQADKP
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DTYVLATNRTETVRDFVTMAAKAVDTEIMWEGHAESEVGYDRKTNKAIVKINPKFYRPAE
CEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEEEECCCEECCCE
VELLIGNPAKAREELGWEPKTTLEQLCQMMVEADVRRNQAGFSF
EEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTAIITGITGQDGAYLAQLLLEKGYTVYGTYRRTSSVNFWRVEELGVDKHPNLHLVEYD
CCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEHCCCCCCCCCEEEEEEE
LTDLSASIRLLQTTKATEVYNLAAQSFVGVSFDQPATTAEITGIGPLNLLEAIRIVNPEI
CHHHHHHEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCE
RFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLYAHWITVNYRESYNIFGSSGILFN
EEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEEEEECCCEEEECCCEEEC
HESPLRGREFVTRKITDSLAKIHLGKLDVLELGNLDAKRDWGFAKEYVEGMWRMLQADKP
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DTYVLATNRTETVRDFVTMAAKAVDTEIMWEGHAESEVGYDRKTNKAIVKINPKFYRPAE
CEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEEEECCCEECCCE
VELLIGNPAKAREELGWEPKTTLEQLCQMMVEADVRRNQAGFSF
EEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]