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The map label for this gene is emrB [H]

Identifier: 187928215

GI number: 187928215

Start: 1197992

End: 1199548

Strand: Reverse

Name: emrB [H]

Synonym: Rpic_1122

Alternate gene names: 187928215

Gene position: 1199548-1197992 (Counterclockwise)

Preceding gene: 187928216

Following gene: 187928204

Centisome position: 30.43

GC content: 62.3

Gene sequence:

>1557_bases
ATGGCAACCGCCGCGCCCAAGCCGCTACAGCCACTGACCGGCGCCAAGCTGGCGATCGGTACCGTCGCGTTGTCGCTGGC
CACCTTCATGAACGTGCTGGATTCGTCCATCGCCAACGTGTCGATCCCGGCCATTTCGGGCGACCTGGGCGTGGCCCCGA
ACCAGGGCACGTGGGTCATCACCTCGTTTGCGGTGGCCAACGCCATCTCGGTGCCGCTCACGGGGTGGTTGACGCAGCGT
TTCGGGCAAGTGCGCCTGTTCGTCACGTCGATCCTGCTGTTCGTGTTGTCGTCTTGGGCGTGCGGGCTGGCGCCCAATAT
GGGCATGCTGATCGCCGCCCGGATCATCCAGGGCGCTGTGGCGGGCCCAATGATTCCGCTGTCGCAATCGTTGCTGCTGT
CGACCTATCCGCCCGCCAAAAGCTCAATGGCGTTGGCGCTGTGGGGTATGACGACGCTGGTCGCGCCAATCATGGGCCCG
ATCTTCGGCGGCTGGATCTCCGACAACATGAGCTGGCCCTGGATCTTCTACATCAACATCCCGGTGGGCATCCTGGCTGC
CTATGCGACGTGGGTCATCTACAAGGACCGTGAATCGCCGACACGTTCGCTGCCCATCGACAAGATCGGCCTGGCGCTGT
TGATCCTGTGGGTCGGCTCGCTTCAGTTGATGCTCGATCGCGGCAAGGAACTCGACTGGTTCAACTCGACCGAAGTCGTG
GTGCTGACGGTCGTCGCCATCGTGGGCTTCGCGTTCTTCCTCATATGGGAGCTGACCGAAGATCACCCCGTGGTGGATCT
GACGCTGTTCAAGGGCCGCAACTTCAGCGCGGGTGTCGTCGCCATCTCCGTGGCGTACGGCCTGTTCTTCGGCAACCTCG
TGATCCTGCCGCTATGGCTGCAGACCATCGTCGGCTACACCGCCACCGATGCGGGCCTGATCATGGCACCGGTGGGCATC
TTCGCCATCATTCTCTCGCCCGTGATCGGCAAGAACCTGCCGAAGATGGATGCACGCTGGGTCGCGACCACGGCGTTTAT
GACCTTCGCGCTGGTGTTCTGGATGCGCTCGCGCTTTACCGTACAGGTAGATACGTGGACGCTGATGATCCCGACGCTGA
TCCAGGGCGCGGCCATGGCGATGTTCTTCATCCCGCTCACGTCGATCATCCTGTCGGGTCTGTCGCCCGAGCGGATTCCG
GCCGCCTCCGGGCTATCGAACTTTGTGCGGATCATGTTCGGCGGTATCGGTGCATCGATCTCGACCACGGTGTGGGACAA
CCGCTCCGCCCTGCACCATGCGCAACTGGTTGAACACGTGAATCCGTACAACCCGGCCTATGCGTCGTCGATCAACCAGT
ACACGCAATTGGGCATGCCCAACCTGCAGGCCCATGGCGTGATCGAACGTGTGATCTCGCAGCAGGCCGCAATGCTGGGC
GCCAACGATATCTTCTGGATTTCGGCCGTGCTGTTTATCGTGCTGATCGTCTTCATCTGGCTGACCAAGCCGGCGAAGTC
GGCCGCCGGCGCAGAAGCTGCAGCCGGCGCGCACTGA

Upstream 100 bases:

>100_bases
CGCCAACGCTGGCCGAGGCGGCGCCACGGCACCGGCCGCCGCCAACGTGCCGGCCGCATCGGCTCCGCGCACTTCGCAAC
CCGCACCCAAGGTCTGACGC

Downstream 100 bases:

>100_bases
TCCAAGATCGCGCGTGTCAAGTCCTGCCATAGGTTGACACTGTGCTCAGTCTCCGAGCGCCCGATGCACCGCATCGGGCG
TTTTGTATTTCAAGGCCAGA

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MATAAPKPLQPLTGAKLAIGTVALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVITSFAVANAISVPLTGWLTQR
FGQVRLFVTSILLFVLSSWACGLAPNMGMLIAARIIQGAVAGPMIPLSQSLLLSTYPPAKSSMALALWGMTTLVAPIMGP
IFGGWISDNMSWPWIFYINIPVGILAAYATWVIYKDRESPTRSLPIDKIGLALLILWVGSLQLMLDRGKELDWFNSTEVV
VLTVVAIVGFAFFLIWELTEDHPVVDLTLFKGRNFSAGVVAISVAYGLFFGNLVILPLWLQTIVGYTATDAGLIMAPVGI
FAIILSPVIGKNLPKMDARWVATTAFMTFALVFWMRSRFTVQVDTWTLMIPTLIQGAAMAMFFIPLTSIILSGLSPERIP
AASGLSNFVRIMFGGIGASISTTVWDNRSALHHAQLVEHVNPYNPAYASSINQYTQLGMPNLQAHGVIERVISQQAAMLG
ANDIFWISAVLFIVLIVFIWLTKPAKSAAGAEAAAGAH

Sequences:

>Translated_518_residues
MATAAPKPLQPLTGAKLAIGTVALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVITSFAVANAISVPLTGWLTQR
FGQVRLFVTSILLFVLSSWACGLAPNMGMLIAARIIQGAVAGPMIPLSQSLLLSTYPPAKSSMALALWGMTTLVAPIMGP
IFGGWISDNMSWPWIFYINIPVGILAAYATWVIYKDRESPTRSLPIDKIGLALLILWVGSLQLMLDRGKELDWFNSTEVV
VLTVVAIVGFAFFLIWELTEDHPVVDLTLFKGRNFSAGVVAISVAYGLFFGNLVILPLWLQTIVGYTATDAGLIMAPVGI
FAIILSPVIGKNLPKMDARWVATTAFMTFALVFWMRSRFTVQVDTWTLMIPTLIQGAAMAMFFIPLTSIILSGLSPERIP
AASGLSNFVRIMFGGIGASISTTVWDNRSALHHAQLVEHVNPYNPAYASSINQYTQLGMPNLQAHGVIERVISQQAAMLG
ANDIFWISAVLFIVLIVFIWLTKPAKSAAGAEAAAGAH
>Mature_517_residues
ATAAPKPLQPLTGAKLAIGTVALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVITSFAVANAISVPLTGWLTQRF
GQVRLFVTSILLFVLSSWACGLAPNMGMLIAARIIQGAVAGPMIPLSQSLLLSTYPPAKSSMALALWGMTTLVAPIMGPI
FGGWISDNMSWPWIFYINIPVGILAAYATWVIYKDRESPTRSLPIDKIGLALLILWVGSLQLMLDRGKELDWFNSTEVVV
LTVVAIVGFAFFLIWELTEDHPVVDLTLFKGRNFSAGVVAISVAYGLFFGNLVILPLWLQTIVGYTATDAGLIMAPVGIF
AIILSPVIGKNLPKMDARWVATTAFMTFALVFWMRSRFTVQVDTWTLMIPTLIQGAAMAMFFIPLTSIILSGLSPERIPA
ASGLSNFVRIMFGGIGASISTTVWDNRSALHHAQLVEHVNPYNPAYASSINQYTQLGMPNLQAHGVIERVISQQAAMLGA
NDIFWISAVLFIVLIVFIWLTKPAKSAAGAEAAAGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=496, Percent_Identity=56.25, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1788710, Length=517, Percent_Identity=46.2282398452611, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1790195, Length=424, Percent_Identity=28.7735849056604, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87081983, Length=403, Percent_Identity=26.302729528536, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788392, Length=389, Percent_Identity=29.5629820051414, Blast_Score=118, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6322958, Length=552, Percent_Identity=22.1014492753623, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=27.1317829457364, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55695; Mature: 55564

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAAPKPLQPLTGAKLAIGTVALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCEECCCCCCCCCCCCEEE
TSFAVANAISVPLTGWLTQRFGQVRLFVTSILLFVLSSWACGLAPNMGMLIAARIIQGAV
EHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AGPMIPLSQSLLLSTYPPAKSSMALALWGMTTLVAPIMGPIFGGWISDNMSWPWIFYINI
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEEC
PVGILAAYATWVIYKDRESPTRSLPIDKIGLALLILWVGSLQLMLDRGKELDWFNSTEVV
CHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
VLTVVAIVGFAFFLIWELTEDHPVVDLTLFKGRNFSAGVVAISVAYGLFFGNLVILPLWL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QTIVGYTATDAGLIMAPVGIFAIILSPVIGKNLPKMDARWVATTAFMTFALVFWMRSRFT
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEE
VQVDTWTLMIPTLIQGAAMAMFFIPLTSIILSGLSPERIPAASGLSNFVRIMFGGIGASI
EEEEHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
STTVWDNRSALHHAQLVEHVNPYNPAYASSINQYTQLGMPNLQAHGVIERVISQQAAMLG
EEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
ANDIFWISAVLFIVLIVFIWLTKPAKSAAGAEAAAGAH
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATAAPKPLQPLTGAKLAIGTVALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCEECCCCCCCCCCCCEEE
TSFAVANAISVPLTGWLTQRFGQVRLFVTSILLFVLSSWACGLAPNMGMLIAARIIQGAV
EHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AGPMIPLSQSLLLSTYPPAKSSMALALWGMTTLVAPIMGPIFGGWISDNMSWPWIFYINI
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEEC
PVGILAAYATWVIYKDRESPTRSLPIDKIGLALLILWVGSLQLMLDRGKELDWFNSTEVV
CHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
VLTVVAIVGFAFFLIWELTEDHPVVDLTLFKGRNFSAGVVAISVAYGLFFGNLVILPLWL
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QTIVGYTATDAGLIMAPVGIFAIILSPVIGKNLPKMDARWVATTAFMTFALVFWMRSRFT
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEE
VQVDTWTLMIPTLIQGAAMAMFFIPLTSIILSGLSPERIPAASGLSNFVRIMFGGIGASI
EEEEHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
STTVWDNRSALHHAQLVEHVNPYNPAYASSINQYTQLGMPNLQAHGVIERVISQQAAMLG
EEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
ANDIFWISAVLFIVLIVFIWLTKPAKSAAGAEAAAGAH
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]