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The map label for this gene is emrA [H]

Identifier: 187928216

GI number: 187928216

Start: 1199552

End: 1200841

Strand: Reverse

Name: emrA [H]

Synonym: Rpic_1123

Alternate gene names: 187928216

Gene position: 1200841-1199552 (Counterclockwise)

Preceding gene: 187928217

Following gene: 187928215

Centisome position: 30.46

GC content: 67.05

Gene sequence:

>1290_bases
ATGAGTGACAACAAGCCCCAAGCCGCACCGTCCGCTGCTCCCGCGCCCGCAGCCCCCGCGGCAACGCCTACCGGCAACGG
CAACGGCAACGGCAAGCGCAAGCGCATGCTGACCACGCTGGCCGCCGCACTGGTGGTTGCCGGCGTCGGCTACGGCCTCT
ACTGGGGCCTGTACGGCCGCTGGTTCGAGTCGACCGACGATGCATACGTGCAAGGCAACGTGGTCCAGGTGACACCGCAA
GTGGCTGGTACCGTGGTCGCCATTCGCGCCGATGACACGCAACTCGTCACCTCTGGCCAGCCCGTGATCGAACTGGACCG
GGCCGATGCACGCGTCGCGCTGGAGCAGGCCGAAGCCGCGCTCGCACAAACGGTGCGCCAGGTGCGCACGCTGTACTCGA
ACACCAGCGCGTTCACCGCCACGATGGCCATGCGTGAAAGCGATCTCGCCAAGGCCAAGGATGACTTGGCCCGCCGCAAG
CAGATCGCCGGTACGGGCGCCGTGTCGCAGGAAGAAATCTCGCACGCACAAACCGCCGTGCAGGCTGCGCAGTCCGCGCT
GGAAGCCGCCAAGGAACAGCTGCAAGGCAACCGTGTGCTGACGGAACAGACCACGCTGGAGCGTCATCCGAACGTGCTGC
AGGCTGCCGCCAAGGTCCGCGAGGCTTACCTGGCCTATGCGCGTACTAGCCTGCCGGCATCGGTCACGGGCTATGTGGCC
AAGCGTTCGGTGCAGGTCGGCCAGCGCGTCGCTCCTGGCACGCCGCTGATGGCCATCGTGCCGCTGGATCAGCTCTGGGT
GGACGCCAACTTCAAGGAAGTCCAGGTGCGCCACATGCGCGTGGGCCAGCCGGTTGAACTGGTGGCCGACGTGTATGGCA
GCAGCGTGACGTACCACGGCAAGGTGGTCGGTTTCTCGGCGGGTACGGGCTCGGCGTTCTCGCTGTTGCCGGCACAGAAC
GCCACCGGCAACTGGATCAAGGTCGTGCAGCGCCTGCCGGTGCGCGTGTCGCTCGATCCAAAGGAATTGAAGGACCATCC
GCTGCGCGTGGGTCTGTCGATGGAAGCCAAGGTCGACATCCATGACGAAGGCGGGCAGAGCCTGGCCACCGCACCGGCTG
CGTCGCCGCTACAAACGACGGTGTACGACCAGGCCGGCAAGGACGCAGATCAGGTCATCGCCAGCATCATCGCCGCCAAC
GCTGGCCGAGGCGGCGCCACGGCACCGGCCGCCGCCAACGTGCCGGCCGCATCGGCTCCGCGCACTTCGCAACCCGCACC
CAAGGTCTGA

Upstream 100 bases:

>100_bases
ACCTGCCGGGCGCCAAGCCTGAGGCCGCCGCCTCGCATGGCTGACCTGTACTGAATTGAACTGAACCGACCGTTCCAACG
CATTCTTTCGAGCCAACATC

Downstream 100 bases:

>100_bases
CGCATGGCAACCGCCGCGCCCAAGCCGCTACAGCCACTGACCGGCGCCAAGCTGGCGATCGGTACCGTCGCGTTGTCGCT
GGCCACCTTCATGAACGTGC

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSDNKPQAAPSAAPAPAAPAATPTGNGNGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGRWFESTDDAYVQGNVVQVTPQ
VAGTVVAIRADDTQLVTSGQPVIELDRADARVALEQAEAALAQTVRQVRTLYSNTSAFTATMAMRESDLAKAKDDLARRK
QIAGTGAVSQEEISHAQTAVQAAQSALEAAKEQLQGNRVLTEQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVA
KRSVQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHGKVVGFSAGTGSAFSLLPAQN
ATGNWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEAKVDIHDEGGQSLATAPAASPLQTTVYDQAGKDADQVIASIIAAN
AGRGGATAPAAANVPAASAPRTSQPAPKV

Sequences:

>Translated_429_residues
MSDNKPQAAPSAAPAPAAPAATPTGNGNGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGRWFESTDDAYVQGNVVQVTPQ
VAGTVVAIRADDTQLVTSGQPVIELDRADARVALEQAEAALAQTVRQVRTLYSNTSAFTATMAMRESDLAKAKDDLARRK
QIAGTGAVSQEEISHAQTAVQAAQSALEAAKEQLQGNRVLTEQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVA
KRSVQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHGKVVGFSAGTGSAFSLLPAQN
ATGNWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEAKVDIHDEGGQSLATAPAASPLQTTVYDQAGKDADQVIASIIAAN
AGRGGATAPAAANVPAASAPRTSQPAPKV
>Mature_428_residues
SDNKPQAAPSAAPAPAAPAATPTGNGNGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGRWFESTDDAYVQGNVVQVTPQV
AGTVVAIRADDTQLVTSGQPVIELDRADARVALEQAEAALAQTVRQVRTLYSNTSAFTATMAMRESDLAKAKDDLARRKQ
IAGTGAVSQEEISHAQTAVQAAQSALEAAKEQLQGNRVLTEQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVAK
RSVQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHGKVVGFSAGTGSAFSLLPAQNA
TGNWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEAKVDIHDEGGQSLATAPAASPLQTTVYDQAGKDADQVIASIIAANA
GRGGATAPAAANVPAASAPRTSQPAPKV

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=386, Percent_Identity=46.3730569948187, Blast_Score=345, Evalue=4e-96,
Organism=Escherichia coli, GI1788711, Length=339, Percent_Identity=45.4277286135693, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI87081951, Length=315, Percent_Identity=26.984126984127, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790519, Length=313, Percent_Identity=27.4760383386581, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789637, Length=325, Percent_Identity=25.2307692307692, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44894; Mature: 44763

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNKPQAAPSAAPAPAAPAATPTGNGNGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WFESTDDAYVQGNVVQVTPQVAGTVVAIRADDTQLVTSGQPVIELDRADARVALEQAEAA
HHCCCCCCEEECCEEEECCCCCCEEEEEEECCCEEEECCCCEEEECCCCHHHHHHHHHHH
LAQTVRQVRTLYSNTSAFTATMAMRESDLAKAKDDLARRKQIAGTGAVSQEEISHAQTAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
QAAQSALEAAKEQLQGNRVLTEQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVA
HHHHHHHHHHHHHHCCCEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KRSVQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHG
HHHHHHCCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEE
KVVGFSAGTGSAFSLLPAQNATGNWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEAKVDI
EEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCEEECCHHHHCCCCEEEEEEEEEEEEE
HDEGGQSLATAPAASPLQTTVYDQAGKDADQVIASIIAANAGRGGATAPAAANVPAASAP
ECCCCCEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
RTSQPAPKV
CCCCCCCCC
>Mature Secondary Structure 
SDNKPQAAPSAAPAPAAPAATPTGNGNGNGKRKRMLTTLAAALVVAGVGYGLYWGLYGR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WFESTDDAYVQGNVVQVTPQVAGTVVAIRADDTQLVTSGQPVIELDRADARVALEQAEAA
HHCCCCCCEEECCEEEECCCCCCEEEEEEECCCEEEECCCCEEEECCCCHHHHHHHHHHH
LAQTVRQVRTLYSNTSAFTATMAMRESDLAKAKDDLARRKQIAGTGAVSQEEISHAQTAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
QAAQSALEAAKEQLQGNRVLTEQTTLERHPNVLQAAAKVREAYLAYARTSLPASVTGYVA
HHHHHHHHHHHHHHCCCEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KRSVQVGQRVAPGTPLMAIVPLDQLWVDANFKEVQVRHMRVGQPVELVADVYGSSVTYHG
HHHHHHCCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEE
KVVGFSAGTGSAFSLLPAQNATGNWIKVVQRLPVRVSLDPKELKDHPLRVGLSMEAKVDI
EEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCEEECCHHHHCCCCEEEEEEEEEEEEE
HDEGGQSLATAPAASPLQTTVYDQAGKDADQVIASIIAANAGRGGATAPAAANVPAASAP
ECCCCCEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
RTSQPAPKV
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]