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The map label for this gene is rluB [H]

Identifier: 187928207

GI number: 187928207

Start: 1188243

End: 1190027

Strand: Direct

Name: rluB [H]

Synonym: Rpic_1114

Alternate gene names: 187928207

Gene position: 1188243-1190027 (Clockwise)

Preceding gene: 187928206

Following gene: 187928208

Centisome position: 30.14

GC content: 66.67

Gene sequence:

>1785_bases
ATGCCGGCCGACGCCGACGCTCAGCCGCCGCGACAAGACGGCGGTGATGGGACTGAGGCCACCCCTCGCCGCAAGGGATT
GCGCCGAGGCTTGCGCAACCTCGTGGCTTCGCGCCGCCAGCAGCAGGATGGTCGCCCTGAGGGCGAAGCCGCCGATGCGG
GTGGTGCAGACGCCGGCCAGCCCGCGCAACCGCGCAAGACCCGCGCGCCACGGGCGCGCAAGCCGAAAGAGGCGGCTGGT
GAGCCGGCGGTAGTTGCTGCAGAAGGTGCTGCGACGGCCGAACAGGCGCCAGCAGAAAACCAGGGCGAGAGACGTGGCCC
GCGCGGCCGTTTCGGTAAGAACCGCCGCCGTCAAGGCGAAGGTCAGCCGCTGGAGCGCGCCGAGCGCAACGACCGTGTGG
AAGGTGCTGGCGGTAAGAACACCGGGGGTCGTGGCAAGTCGCAGAACCGCAACGGCAAACCGCAAGGCAAGGCAGGCGGC
GCGAACCAGAATCGCGGGGGCAAGGGGCTGAAGGGAGGCAAGGGCTCTGAAGACGTCTTCCAATACGTGATTTCCGGGGC
GTACGACTCCGAGGCTGACACCGGCGGTGCATCGCAGCCTCCCAAGGCGCGCGAACTGACCTCCGAAGACGACGCTCCCA
AACTGCACAAAATCCTGGCTGATGCCGGGCTTGGCTCTCGCCGCGACATGGAAGACCTGATCCTGCAAGGGCGCGTCTCC
GTGAATGGCCTGCCGGCTCATATCGGTCAACGCATTCTCGCGACCGACCAGGTGCGCGTGAACGGCAAGCTGATCCAGCG
CAAGTTGCCGAACAAGCCGCCGCGCGTGCTGCTGTATCACAAGCCGTCGGGTGAAATCGTCAGCCAGTCCGATCCGGAAG
GTCGTCCGACGGTGTTCGACGCGTTGCCGCGCATGAAGACCGGCAAGTGGGTCGCCGTCGGCCGCCTCGACTTCAATACC
GAGGGCCTGCTGATTTTCACGACCTCCGGCGATATCGCCAACCGGTTCATGCACCCGCGCTATGGTGTTGAGCGTGAATA
CGCGGTGCGTACGCTGGGTGAGCTGGCCGAAACTGACCGTCAGAAGCTGTTGCACGGCATCTCGCTCCAGGACGGCGAAG
CCAACTTCCTGCGCTGCGTCGATGGCGGCGGCGAAGGTGTCAACCACTGGTACCACGTCGCCCTGACCGAGGGGCGGAAC
CGCGAAGTGCGGCGCATGTTTGAGGCGGTCAACCTGACCGTGTCGCGGCTGATCCGGACGCGCTATGGCAGCTTCGTACT
GCCGCGCGGCCTTAAGCGGGGCCGTTGGCAGGAGGTGTCGGCCGAGGATGTGCGTACGCTGATGGGCACGCTGGGCATGA
AGGTGCCGACGGCGAGTTCTGGCGGTGGGCAAGACCGCCAGGGCAATCGTCGCCGTGGTCCGGCGCCGGTGCTGATGGGG
CCGATGTCCTCTGGTTTTGCGGGGGAAGCCAACTTCCAGTCGCGTGGTATGGGCGTTGAAAACGGTAATCGCGCGCAACC
CGCGCAGCGCCGTCCGTCCAGCCCGCGTCAGCCGGATCCGATGCAGACGTCCATGGGCTACATCAATGTGGGAGGCCCGA
CCACGCTCACAGCGCGAACTCGTATGGGCGATCGTGCGAACGGCCGTGGCGGTGCGGGGGCGGGTGGTGGCATGCGTGAT
GGCAATCGCGCACCCGGCAACAAGCGCCCCGGAAAGAGCGCTGGCGGCATGCCGAACGGCAACAAGGCGCCACGCGGTGG
TGGCAAACGCGGCGGTAAGCGTTAA

Upstream 100 bases:

>100_bases
ACATTCGTAATGCCATGCCCGCCGTGCAGTCGGCGGATGGCTTGAACCCCTAGATTTGAAAGACGTTGTGAGCACCCAGC
CAGATTCCATGGATACACGT

Downstream 100 bases:

>100_bases
AAATGCGGGCGTTGTGTGCGGCATTCGTCGCAACGCCCCATTTTGTGGCGGCTTCGGGGCAATTTTTGCCTTTTGCCTCG
AAGCGTATTACAATGTGAGT

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MPADADAQPPRQDGGDGTEATPRRKGLRRGLRNLVASRRQQQDGRPEGEAADAGGADAGQPAQPRKTRAPRARKPKEAAG
EPAVVAAEGAATAEQAPAENQGERRGPRGRFGKNRRRQGEGQPLERAERNDRVEGAGGKNTGGRGKSQNRNGKPQGKAGG
ANQNRGGKGLKGGKGSEDVFQYVISGAYDSEADTGGASQPPKARELTSEDDAPKLHKILADAGLGSRRDMEDLILQGRVS
VNGLPAHIGQRILATDQVRVNGKLIQRKLPNKPPRVLLYHKPSGEIVSQSDPEGRPTVFDALPRMKTGKWVAVGRLDFNT
EGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAETDRQKLLHGISLQDGEANFLRCVDGGGEGVNHWYHVALTEGRN
REVRRMFEAVNLTVSRLIRTRYGSFVLPRGLKRGRWQEVSAEDVRTLMGTLGMKVPTASSGGGQDRQGNRRRGPAPVLMG
PMSSGFAGEANFQSRGMGVENGNRAQPAQRRPSSPRQPDPMQTSMGYINVGGPTTLTARTRMGDRANGRGGAGAGGGMRD
GNRAPGNKRPGKSAGGMPNGNKAPRGGGKRGGKR

Sequences:

>Translated_594_residues
MPADADAQPPRQDGGDGTEATPRRKGLRRGLRNLVASRRQQQDGRPEGEAADAGGADAGQPAQPRKTRAPRARKPKEAAG
EPAVVAAEGAATAEQAPAENQGERRGPRGRFGKNRRRQGEGQPLERAERNDRVEGAGGKNTGGRGKSQNRNGKPQGKAGG
ANQNRGGKGLKGGKGSEDVFQYVISGAYDSEADTGGASQPPKARELTSEDDAPKLHKILADAGLGSRRDMEDLILQGRVS
VNGLPAHIGQRILATDQVRVNGKLIQRKLPNKPPRVLLYHKPSGEIVSQSDPEGRPTVFDALPRMKTGKWVAVGRLDFNT
EGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAETDRQKLLHGISLQDGEANFLRCVDGGGEGVNHWYHVALTEGRN
REVRRMFEAVNLTVSRLIRTRYGSFVLPRGLKRGRWQEVSAEDVRTLMGTLGMKVPTASSGGGQDRQGNRRRGPAPVLMG
PMSSGFAGEANFQSRGMGVENGNRAQPAQRRPSSPRQPDPMQTSMGYINVGGPTTLTARTRMGDRANGRGGAGAGGGMRD
GNRAPGNKRPGKSAGGMPNGNKAPRGGGKRGGKR
>Mature_593_residues
PADADAQPPRQDGGDGTEATPRRKGLRRGLRNLVASRRQQQDGRPEGEAADAGGADAGQPAQPRKTRAPRARKPKEAAGE
PAVVAAEGAATAEQAPAENQGERRGPRGRFGKNRRRQGEGQPLERAERNDRVEGAGGKNTGGRGKSQNRNGKPQGKAGGA
NQNRGGKGLKGGKGSEDVFQYVISGAYDSEADTGGASQPPKARELTSEDDAPKLHKILADAGLGSRRDMEDLILQGRVSV
NGLPAHIGQRILATDQVRVNGKLIQRKLPNKPPRVLLYHKPSGEIVSQSDPEGRPTVFDALPRMKTGKWVAVGRLDFNTE
GLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAETDRQKLLHGISLQDGEANFLRCVDGGGEGVNHWYHVALTEGRNR
EVRRMFEAVNLTVSRLIRTRYGSFVLPRGLKRGRWQEVSAEDVRTLMGTLGMKVPTASSGGGQDRQGNRRRGPAPVLMGP
MSSGFAGEANFQSRGMGVENGNRAQPAQRRPSSPRQPDPMQTSMGYINVGGPTTLTARTRMGDRANGRGGAGAGGGMRDG
NRAPGNKRPGKSAGGMPNGNKAPRGGGKRGGKR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=251, Percent_Identity=50.996015936255, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=28.0632411067194, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI87081838, Length=194, Percent_Identity=31.4432989690722, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788510, Length=179, Percent_Identity=28.4916201117318, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 63426; Mature: 63295

Theoretical pI: Translated: 11.44; Mature: 11.44

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPADADAQPPRQDGGDGTEATPRRKGLRRGLRNLVASRRQQQDGRPEGEAADAGGADAGQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PAQPRKTRAPRARKPKEAAGEPAVVAAEGAATAEQAPAENQGERRGPRGRFGKNRRRQGE
CCCCCHHCCCCCCCCHHHCCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCHHCCCC
GQPLERAERNDRVEGAGGKNTGGRGKSQNRNGKPQGKAGGANQNRGGKGLKGGKGSEDVF
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
QYVISGAYDSEADTGGASQPPKARELTSEDDAPKLHKILADAGLGSRRDMEDLILQGRVS
HHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCC
VNGLPAHIGQRILATDQVRVNGKLIQRKLPNKPPRVLLYHKPSGEIVSQSDPEGRPTVFD
CCCCHHHHCCHHHHCCEEEECHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHH
ALPRMKTGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAETDR
HHCCCCCCCEEEEEEEEECCCCEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QKLLHGISLQDGEANFLRCVDGGGEGVNHWYHVALTEGRNREVRRMFEAVNLTVSRLIRT
HHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RYGSFVLPRGLKRGRWQEVSAEDVRTLMGTLGMKVPTASSGGGQDRQGNRRRGPAPVLMG
HHCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEC
PMSSGFAGEANFQSRGMGVENGNRAQPAQRRPSSPRQPDPMQTSMGYINVGGPTTLTART
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHCCCCCCCCCCCHHHCCCEEECCCCEEEEEHH
RMGDRANGRGGAGAGGGMRDGNRAPGNKRPGKSAGGMPNGNKAPRGGGKRGGKR
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PADADAQPPRQDGGDGTEATPRRKGLRRGLRNLVASRRQQQDGRPEGEAADAGGADAGQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PAQPRKTRAPRARKPKEAAGEPAVVAAEGAATAEQAPAENQGERRGPRGRFGKNRRRQGE
CCCCCHHCCCCCCCCHHHCCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCHHCCCC
GQPLERAERNDRVEGAGGKNTGGRGKSQNRNGKPQGKAGGANQNRGGKGLKGGKGSEDVF
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
QYVISGAYDSEADTGGASQPPKARELTSEDDAPKLHKILADAGLGSRRDMEDLILQGRVS
HHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCC
VNGLPAHIGQRILATDQVRVNGKLIQRKLPNKPPRVLLYHKPSGEIVSQSDPEGRPTVFD
CCCCHHHHCCHHHHCCEEEECHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHH
ALPRMKTGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAETDR
HHCCCCCCCEEEEEEEEECCCCEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
QKLLHGISLQDGEANFLRCVDGGGEGVNHWYHVALTEGRNREVRRMFEAVNLTVSRLIRT
HHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RYGSFVLPRGLKRGRWQEVSAEDVRTLMGTLGMKVPTASSGGGQDRQGNRRRGPAPVLMG
HHCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEC
PMSSGFAGEANFQSRGMGVENGNRAQPAQRRPSSPRQPDPMQTSMGYINVGGPTTLTART
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHCCCCCCCCCCCHHHCCCEEECCCCEEEEEHH
RMGDRANGRGGAGAGGGMRDGNRAPGNKRPGKSAGGMPNGNKAPRGGGKRGGKR
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]