Click here to switch to the map view.

The map label for this gene is sucB [H]

Identifier: 187928191

GI number: 187928191

Start: 1172093

End: 1173343

Strand: Direct

Name: sucB [H]

Synonym: Rpic_1098

Alternate gene names: 187928191

Gene position: 1172093-1173343 (Clockwise)

Preceding gene: 187928190

Following gene: 187928192

Centisome position: 29.73

GC content: 65.07

Gene sequence:

>1251_bases
ATGGCTATCGTTGAAGTCAAGGTCCCGCAGTTTTCCGAGTCCGTTGAAGAAGGCACGCTGATCTCCTGGAAGAAGAAGCC
GGGCGAAGCCGTGGCCCAGGACGAAATCCTGATCGAAGTCGAGACCGACAAGGTCGTGCTGGAAGTGCCGGCCCCGTCGG
CTGGCGTGCTGGCTGAAGTGCTGGTGGCTGACGGCGCAACCGTGACGTCGGAGCAGCTGCTGGCCAAGATCGACACCGAA
GGCAAGGCAGGCGCCGCCGCTCCGGCTGCTGCTGCTCCGGCTCCCGCAGCCGCTGCACCGGCACCGGTTGCTGCTCCGGC
CGCCGCCGCTACGGGTGGCGTGGCCATGCCGTCGGCTGCCAAGCTGATGGCTGAGAACAACCTGTCGGCTGGCCAGGTGG
CCGGCACGGGCCGCGATGGCCGCATCACCAAGGGCGACGTGCTGGGTGCCGTAGCCGGTGGCGCCAAGCCGGCTGCTGCT
GCCGCTCCGCAGGCTGCACGTCCGGCGCTGCAGCAAGTGGCTGCACCGGTGGACTTCGCCGCCCTGGGCGACCGTCCGGA
AGAGCGCGTGCCGATGAGCCGCCTGCGCGCCCGTATTGCCGAGCGCCTGGTGCAGTCGCAATCGACCAACGCCATCCTCA
CCACCTTCAACGAAGTCAACATGAAGCCGGTGATGGACTTGCGCGCCAAGTTCAAGGACCAGTTCGAGAAGACTCACGGC
GTGAAGCTGGGCTTCATGTCGTTCTTCGTGAAGGCGGCTGTCCACGCGCTGAAGAAGTACCCCGTGATCAACGCATCGGT
TGACGGCAACGACATCGTCTACCACGGCTACTTCGACATCGGTATCGCTGTTGGCTCGCCGCGCGGCCTGGTGGTGCCCA
TCCTGCGCAATGCCGACCAGATGAGCCTGGCCGATATCGAGAAGAAGATTGCTGAATTCGGCCAGAAGGCCAAGGACGGC
AAGCTGACGCTGGACGACCTGACCGGCGGTACGTTCTCGATCTCGAACGGCGGCACGTTCGGCTCGATGCTGTCGACCCC
GATCATCAACCCGCCGCAATCGGCCATCCTGGGCGTGCACGCCACCAAGGATCGCGCCGTAGTGGAGAACGGCCAGGTCG
TGGTTCGCCCGATGAACTACCTGGCCATGTCCTACGACCACCGCATCATCGACGGCCGCGAAGCCGTTCTGGGTCTGGTG
GCGATGAAGGAAGCGCTGGAAGACCCGGCCCGCCTGCTGCTGGATCTCTAA

Upstream 100 bases:

>100_bases
CGCCTTGACGCATCTTCGCTCCCGAATTCCACCGTCTATCGAATTAGCGCGGCTCCCGAACGAGAGGAGCACGTGCACAT
GAATCCAAGGAAGCACCGAA

Downstream 100 bases:

>100_bases
TCGACCGCGTTGATGACACGAGACGCCGCCCACGCTGTGCGGCGGCGTCCGAATCGAGGATTTCTCCATGAGCAAAGAAT
TTGACGTGCTGGTGATCGGC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MAIVEVKVPQFSESVEEGTLISWKKKPGEAVAQDEILIEVETDKVVLEVPAPSAGVLAEVLVADGATVTSEQLLAKIDTE
GKAGAAAPAAAAPAPAAAAPAPVAAPAAAATGGVAMPSAAKLMAENNLSAGQVAGTGRDGRITKGDVLGAVAGGAKPAAA
AAPQAARPALQQVAAPVDFAALGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRAKFKDQFEKTHG
VKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGQKAKDG
KLTLDDLTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLGLV
AMKEALEDPARLLLDL

Sequences:

>Translated_416_residues
MAIVEVKVPQFSESVEEGTLISWKKKPGEAVAQDEILIEVETDKVVLEVPAPSAGVLAEVLVADGATVTSEQLLAKIDTE
GKAGAAAPAAAAPAPAAAAPAPVAAPAAAATGGVAMPSAAKLMAENNLSAGQVAGTGRDGRITKGDVLGAVAGGAKPAAA
AAPQAARPALQQVAAPVDFAALGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRAKFKDQFEKTHG
VKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGQKAKDG
KLTLDDLTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLGLV
AMKEALEDPARLLLDL
>Mature_415_residues
AIVEVKVPQFSESVEEGTLISWKKKPGEAVAQDEILIEVETDKVVLEVPAPSAGVLAEVLVADGATVTSEQLLAKIDTEG
KAGAAAPAAAAPAPAAAAPAPVAAPAAAATGGVAMPSAAKLMAENNLSAGQVAGTGRDGRITKGDVLGAVAGGAKPAAAA
APQAARPALQQVAAPVDFAALGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRAKFKDQFEKTHGV
KLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGQKAKDGK
LTLDDLTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLGLVA
MKEALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=239, Percent_Identity=55.6485355648536, Blast_Score=282, Evalue=3e-76,
Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=31.7460317460317, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=30.1762114537445, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=30.1762114537445, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI110671329, Length=426, Percent_Identity=27.6995305164319, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=35.4037267080745, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786946, Length=419, Percent_Identity=52.744630071599, Blast_Score=443, Evalue=1e-126,
Organism=Escherichia coli, GI1786305, Length=406, Percent_Identity=32.2660098522167, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=419, Percent_Identity=44.1527446300716, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=33.0357142857143, Blast_Score=187, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=432, Percent_Identity=27.3148148148148, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=31.7460317460317, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=409, Percent_Identity=41.8092909535452, Blast_Score=316, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6324258, Length=455, Percent_Identity=29.010989010989, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321632, Length=181, Percent_Identity=28.1767955801105, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=55.8951965065502, Blast_Score=277, Evalue=9e-75,
Organism=Drosophila melanogaster, GI18859875, Length=433, Percent_Identity=27.7136258660508, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24582497, Length=230, Percent_Identity=31.7391304347826, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20129315, Length=230, Percent_Identity=31.7391304347826, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43233; Mature: 43102

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQFSESVEEGTLISWKKKPGEAVAQDEILIEVETDKVVLEVPAPSAGVLAEV
CEEEEEECCCHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHH
LVADGATVTSEQLLAKIDTEGKAGAAAPAAAAPAPAAAAPAPVAAPAAAATGGVAMPSAA
HHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHH
KLMAENNLSAGQVAGTGRDGRITKGDVLGAVAGGAKPAAAAAPQAARPALQQVAAPVDFA
HHHHCCCCCCCCEECCCCCCCEECCCHHEECCCCCCCCHHCCCHHHHHHHHHHHCCCCHH
ALGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRAKFKDQFEKTHG
HCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
VKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQ
CCHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCCC
MSLADIEKKIAEFGQKAKDGKLTLDDLTGGTFSISNGGTFGSMLSTPIINPPQSAILGVH
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCHHHHHCCCCCCCCHHHEEEEE
ATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLGLVAMKEALEDPARLLLDL
CCCCCEEEECCCEEEEEHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
AIVEVKVPQFSESVEEGTLISWKKKPGEAVAQDEILIEVETDKVVLEVPAPSAGVLAEV
EEEEEECCCHHHHHCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHH
LVADGATVTSEQLLAKIDTEGKAGAAAPAAAAPAPAAAAPAPVAAPAAAATGGVAMPSAA
HHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHH
KLMAENNLSAGQVAGTGRDGRITKGDVLGAVAGGAKPAAAAAPQAARPALQQVAAPVDFA
HHHHCCCCCCCCEECCCCCCCEECCCHHEECCCCCCCCHHCCCHHHHHHHHHHHCCCCHH
ALGDRPEERVPMSRLRARIAERLVQSQSTNAILTTFNEVNMKPVMDLRAKFKDQFEKTHG
HCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
VKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQ
CCHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCCC
MSLADIEKKIAEFGQKAKDGKLTLDDLTGGTFSISNGGTFGSMLSTPIINPPQSAILGVH
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCHHHHHCCCCCCCCHHHEEEEE
ATKDRAVVENGQVVVRPMNYLAMSYDHRIIDGREAVLGLVAMKEALEDPARLLLDL
CCCCCEEEECCCEEEEEHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]