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The map label for this gene is odhL [H]

Identifier: 187928192

GI number: 187928192

Start: 1173411

End: 1174847

Strand: Direct

Name: odhL [H]

Synonym: Rpic_1099

Alternate gene names: 187928192

Gene position: 1173411-1174847 (Clockwise)

Preceding gene: 187928191

Following gene: 187928193

Centisome position: 29.76

GC content: 62.77

Gene sequence:

>1437_bases
ATGAGCAAAGAATTTGACGTGCTGGTGATCGGCGCCGGCCCCGGCGGTTACATCGCCGCCATCCGCGCTGGCCAGCTGGG
CCTGAACGTGGCCTGCTGCGAAGACAACCCCTACGACGATCCGAAGGGCGAGCCGCGCCTGGGTGGCACCTGCCTGAACG
TCGGCTGCATTCCTTCGAAGGCGCTGCTGGCCTCGTCGGAAGAGTTCGAGAACGTGAATCACCACCTGGCCGATCACGGC
ATCACGGTGGATGGCGTCAAGGTCGACGTGGCCAAGATGCTCAAGCGCAAGGACGACATCGTCGGCAAGATGACGAAGGG
TATCGAGTTCCTGTTCCGCAAGAACAAGGTGACGCTGCTCAAGGGCCATGGCAAGTTCGTCGGCAAGACTGACGCCGGCT
ATCAGGTCGAAATCGCAGGCAAGGCCGGCACGGAAGTCGTGACCGCCAAGCACGTGATCATCGCCACGGGCTCGAAGGCT
CGCCATCTGCCGGGCGTGAAGGTCGACAACGTGACCATTGCCGACAACGAAGGCGCGCTGAAATTTGGCGAAGTGCCGAA
GAAGCTGGGCGTGATCGGCGCTGGCGTGATCGGCCTGGAACTAGGCTCGGTGTGGCGCCGTCTGGGTGCCGAAGTGACGA
TTCTCGAAGCGCTGCCGAGCTTCCTCGGTGCGGCGGACGAATCCGTCGCCAAGGAAGCCAACAAGCTGCTGACCAAGCAG
GGCCTGAAGATCAACGTCGGCGTGAAGGTTGGCGAGATCGAATCGTCGGCCAAGGGCGTGAAGGTCAACTACACCGACGC
TGCCGGCGCTGCGCAAGTGCTGGAGTGCGACAAGCTGATCGTGTCGATCGGCCGCGTGCCGAACACCGACAACCTGGGCC
TGGAAGCGATCGGCCTGGCGGCGGATCAGCGTGGCTTTATCGAAGTGGACGACCACTGCGCGACCAAGCTGCCGAACCTG
TGGGCGATCGGCGACGTGGTGCGTGGCCCGATGCTGGCGCACAAGGCAGAAGACGAAGGCGTGGCTGTGGCCGAGCGCAT
CGTCGGCCAAAAACCGCACATCGACTACAACTGCATTCCGTGGGTCATCTACACGTACCCGGAAATTGCATGGGTGGGCA
AGACGGAGCAGCAGCTCAAGGCCGAAGGCCGTGAGACCAAGGCCGGCCAGTTCCCGTTCCTGGCCAACGGCCGTGCGCTG
GGCATGGGCGCATCCGACGGTTTCGTGAAGGTCATCGCCGATGCGAAGACCGATGAAATCCTGGGCGTGCACATCGTCGC
AGCCAACGCCTCGGACCTGGTTGCCGAAGCCGTGGTAGCGATGGAGTTCAAGGCTGCGGCCGAAGACATCGGCCGTATCT
GCCATCCGCACCCGTCGATGTCGGAAGTGATGCGCGAAGCTGCGCTGGCTGTCGACAAGCGTCAGCTCAATATGTAA

Upstream 100 bases:

>100_bases
GAAGACCCGGCCCGCCTGCTGCTGGATCTCTAATCGACCGCGTTGATGACACGAGACGCCGCCCACGCTGTGCGGCGGCG
TCCGAATCGAGGATTTCTCC

Downstream 100 bases:

>100_bases
ATCGCAAGCCGCAAGGCAAGCACGATGCCCGGTGAATCCACAAGATTGCCGGGCATCGGTCCATTTGGGCGCGCAAACGG
GCCCCAAGAAGATCAAGCAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSKEFDVLVIGAGPGGYIAAIRAGQLGLNVACCEDNPYDDPKGEPRLGGTCLNVGCIPSKALLASSEEFENVNHHLADHG
ITVDGVKVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLLKGHGKFVGKTDAGYQVEIAGKAGTEVVTAKHVIIATGSKA
RHLPGVKVDNVTIADNEGALKFGEVPKKLGVIGAGVIGLELGSVWRRLGAEVTILEALPSFLGAADESVAKEANKLLTKQ
GLKINVGVKVGEIESSAKGVKVNYTDAAGAAQVLECDKLIVSIGRVPNTDNLGLEAIGLAADQRGFIEVDDHCATKLPNL
WAIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGRETKAGQFPFLANGRAL
GMGASDGFVKVIADAKTDEILGVHIVAANASDLVAEAVVAMEFKAAAEDIGRICHPHPSMSEVMREAALAVDKRQLNM

Sequences:

>Translated_478_residues
MSKEFDVLVIGAGPGGYIAAIRAGQLGLNVACCEDNPYDDPKGEPRLGGTCLNVGCIPSKALLASSEEFENVNHHLADHG
ITVDGVKVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLLKGHGKFVGKTDAGYQVEIAGKAGTEVVTAKHVIIATGSKA
RHLPGVKVDNVTIADNEGALKFGEVPKKLGVIGAGVIGLELGSVWRRLGAEVTILEALPSFLGAADESVAKEANKLLTKQ
GLKINVGVKVGEIESSAKGVKVNYTDAAGAAQVLECDKLIVSIGRVPNTDNLGLEAIGLAADQRGFIEVDDHCATKLPNL
WAIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGRETKAGQFPFLANGRAL
GMGASDGFVKVIADAKTDEILGVHIVAANASDLVAEAVVAMEFKAAAEDIGRICHPHPSMSEVMREAALAVDKRQLNM
>Mature_477_residues
SKEFDVLVIGAGPGGYIAAIRAGQLGLNVACCEDNPYDDPKGEPRLGGTCLNVGCIPSKALLASSEEFENVNHHLADHGI
TVDGVKVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLLKGHGKFVGKTDAGYQVEIAGKAGTEVVTAKHVIIATGSKAR
HLPGVKVDNVTIADNEGALKFGEVPKKLGVIGAGVIGLELGSVWRRLGAEVTILEALPSFLGAADESVAKEANKLLTKQG
LKINVGVKVGEIESSAKGVKVNYTDAAGAAQVLECDKLIVSIGRVPNTDNLGLEAIGLAADQRGFIEVDDHCATKLPNLW
AIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGRETKAGQFPFLANGRALG
MGASDGFVKVIADAKTDEILGVHIVAANASDLVAEAVVAMEFKAAAEDIGRICHPHPSMSEVMREAALAVDKRQLNM

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=48.6140724946695, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI50301238, Length=473, Percent_Identity=26.8498942917548, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI148277065, Length=442, Percent_Identity=24.8868778280543, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI33519430, Length=442, Percent_Identity=24.8868778280543, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI33519428, Length=442, Percent_Identity=24.8868778280543, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI33519426, Length=442, Percent_Identity=24.8868778280543, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI148277071, Length=442, Percent_Identity=24.8868778280543, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI22035672, Length=469, Percent_Identity=27.7185501066098, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI291045266, Length=441, Percent_Identity=24.9433106575964, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI291045268, Length=436, Percent_Identity=23.394495412844, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1786307, Length=470, Percent_Identity=38.0851063829787, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI87082354, Length=477, Percent_Identity=31.0272536687631, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789915, Length=469, Percent_Identity=27.0788912579957, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87081717, Length=469, Percent_Identity=26.226012793177, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI32565766, Length=471, Percent_Identity=47.9830148619958, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17557007, Length=482, Percent_Identity=27.5933609958506, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=497, Percent_Identity=26.9617706237425, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983429, Length=380, Percent_Identity=28.9473684210526, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=380, Percent_Identity=28.9473684210526, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=200, Percent_Identity=29.5, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=45.397489539749, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6325240, Length=483, Percent_Identity=33.1262939958592, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6325166, Length=478, Percent_Identity=26.9874476987448, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=50.5285412262156, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24640553, Length=496, Percent_Identity=27.2177419354839, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640549, Length=496, Percent_Identity=27.2177419354839, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640551, Length=496, Percent_Identity=27.2177419354839, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17737741, Length=497, Percent_Identity=24.748490945674, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50570; Mature: 50439

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEFDVLVIGAGPGGYIAAIRAGQLGLNVACCEDNPYDDPKGEPRLGGTCLNVGCIPSK
CCCCCCEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCH
ALLASSEEFENVNHHLADHGITVDGVKVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLL
HHHCCCHHHHHHHHHHHHCCEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEE
KGHGKFVGKTDAGYQVEIAGKAGTEVVTAKHVIIATGSKARHLPGVKVDNVTIADNEGAL
ECCCCEEECCCCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCEECCEEEECCCCCE
KFGEVPKKLGVIGAGVIGLELGSVWRRLGAEVTILEALPSFLGAADESVAKEANKLLTKQ
ECCCCCHHHHHEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHC
GLKINVGVKVGEIESSAKGVKVNYTDAAGAAQVLECDKLIVSIGRVPNTDNLGLEAIGLA
CCEEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
ADQRGFIEVDDHCATKLPNLWAIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDYNCIP
CCCCCCEEECCHHHHCCCCCEEHHHHHCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCC
WVIYTYPEIAWVGKTEQQLKAEGRETKAGQFPFLANGRALGMGASDGFVKVIADAKTDEI
EEEEECCCEEECCCCHHHHHHCCCCCCCCCCCEEECCEEEECCCCCCEEEEEECCCCCCE
LGVHIVAANASDLVAEAVVAMEFKAAAEDIGRICHPHPSMSEVMREAALAVDKRQLNM
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKEFDVLVIGAGPGGYIAAIRAGQLGLNVACCEDNPYDDPKGEPRLGGTCLNVGCIPSK
CCCCCEEEEECCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCH
ALLASSEEFENVNHHLADHGITVDGVKVDVAKMLKRKDDIVGKMTKGIEFLFRKNKVTLL
HHHCCCHHHHHHHHHHHHCCEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEE
KGHGKFVGKTDAGYQVEIAGKAGTEVVTAKHVIIATGSKARHLPGVKVDNVTIADNEGAL
ECCCCEEECCCCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCEECCEEEECCCCCE
KFGEVPKKLGVIGAGVIGLELGSVWRRLGAEVTILEALPSFLGAADESVAKEANKLLTKQ
ECCCCCHHHHHEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHC
GLKINVGVKVGEIESSAKGVKVNYTDAAGAAQVLECDKLIVSIGRVPNTDNLGLEAIGLA
CCEEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
ADQRGFIEVDDHCATKLPNLWAIGDVVRGPMLAHKAEDEGVAVAERIVGQKPHIDYNCIP
CCCCCCEEECCHHHHCCCCCEEHHHHHCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCC
WVIYTYPEIAWVGKTEQQLKAEGRETKAGQFPFLANGRALGMGASDGFVKVIADAKTDEI
EEEEECCCEEECCCCHHHHHHCCCCCCCCCCCEEECCEEEECCCCCCEEEEEECCCCCCE
LGVHIVAANASDLVAEAVVAMEFKAAAEDIGRICHPHPSMSEVMREAALAVDKRQLNM
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]