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The map label for this gene is rumA

Identifier: 187928146

GI number: 187928146

Start: 1118986

End: 1120338

Strand: Direct

Name: rumA

Synonym: Rpic_1053

Alternate gene names: 187928146

Gene position: 1118986-1120338 (Clockwise)

Preceding gene: 187928144

Following gene: 187928148

Centisome position: 28.38

GC content: 63.41

Gene sequence:

>1353_bases
ATGCCCGTGGCGGGCCCGCTCGACATCGTCTCGCTGGATGGCGAAGCGCGCGGCATCGGCCGTTTGACCAACGAGGACGG
CACGCCGGGGAAGGTTGTGTTCGTGGAAGGCGCGTTGCCGGGGGAGCGGGTCATGTACCGCAGTCACCGCGTAAAGCCGT
CGTACGAGCAGGCGGGTGTCGTCAACATTCTGCGCCAGTCTGCTTCACGCGTGACGCCGCAGTGCAAGTTCTTTGGAGTG
TGCGGTGGCTGCTCGATGCAGCATCTGGATTCGCGCGCACAGGTTGCGATCAAGCAGCGCGTGCTGGAAGACGACCTCTG
GCATCTGGCCAAGCTGCGTCCCGACGTAGTATTCCGCCCCATTGCTGGCCCGGATTGGGGCTATCGCTATCGCGCCCGTC
TGACCGTGCGCCATGTTGCGGCCAAAGGCGGCGTACTGGTGGGTTTTCACGAGCGAAAGAGCAGCTATGTGGCGGACATG
ACGTCGTGCGAGGTGTTGCCGCCGCACGTGTCCGCGCTGCTGGTTCCGTTGCGCGAATTGGTGGGCCGCCTGTCGATCGT
ACAACGTATGCCGCAGATCGAACTGGCGGTCGGCCAGGATGTAACCGCGCTGGTGTTGCGCAACCTAGAGCCGATTACCG
CTGAGGATGAGGCGGAGCTGCGTGCTTTCGCCGATCATCACAACATCCAGTTCTGGCTGCAGCCGAAGGGCCCGGATACG
GTCTATCCGTTCTATCCGCTCGATCGCACGCTGACGTACACGTTGCCCGAGTTCGGCATCACCATGCCGTTCAAGCCGAC
GGATTTCACGCAGGTCAATCACCAGATCAACCGGGTGCTGATGTCGCGCGCGCTGAAACTGCTCGACGCGCAGCCGGGCG
ATCGGCTGCTCGACCTGTTCTGCGGTATCGGCAATTTCACGCTGCCGATGGCCACACGTGCCCGCGAGGTTATGGGCATC
GAAGGCAGTGAGGCGCTGACGACACGTGCGCTTGCCAACGCCCAGCACAATGGCCTGCACACGAAGACCTCATTCGCTTG
CCGCAATCTGTTTGAGGTGTCGGCTGATGACGTTGCCGCGCTGGGCAAGTTTGATCGTTGGCTGATCGATCCGCCCCGCG
AAGGGGCGCTGGCCGTGTCCAAGGCGCTGGCCGAGCTGTCGCAGAGCGGCGGTGATGCGGCCGATTTGCTGCCGAAGCGC
ATCATCTATGTGTCGTGCAATCCGGCGACGCTGGCGCGCGACGCAGGCTTGCTGGTGCACGAGGCAGGCTACCGACTGGC
GGGTGCGGGCGTGGTGAACATGTTCCCGCACACGTCGCATGTTGAGTCGATGGCGGTGTTCGAGCGCGACTGA

Upstream 100 bases:

>100_bases
GTCTCAAGCTGCACCCAACGTCACGCCTTCCTCTTCTGCTCCAGTAACGGTCGCGCCGGACACGCCGCCGGCCAAGCCTC
GTGGTCGCCGCAAGCCCGAA

Downstream 100 bases:

>100_bases
TCTGAGCGATTCCGCGTCTCACGGACAAAGAAAAACCGGCGCACCAGGCGCCGGTTTTTTTCGCTGCGCTGTTGCCAGCG
TGCTGTGCGTGCTTAGTTAC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPVAGPLDIVSLDGEARGIGRLTNEDGTPGKVVFVEGALPGERVMYRSHRVKPSYEQAGVVNILRQSASRVTPQCKFFGV
CGGCSMQHLDSRAQVAIKQRVLEDDLWHLAKLRPDVVFRPIAGPDWGYRYRARLTVRHVAAKGGVLVGFHERKSSYVADM
TSCEVLPPHVSALLVPLRELVGRLSIVQRMPQIELAVGQDVTALVLRNLEPITAEDEAELRAFADHHNIQFWLQPKGPDT
VYPFYPLDRTLTYTLPEFGITMPFKPTDFTQVNHQINRVLMSRALKLLDAQPGDRLLDLFCGIGNFTLPMATRAREVMGI
EGSEALTTRALANAQHNGLHTKTSFACRNLFEVSADDVAALGKFDRWLIDPPREGALAVSKALAELSQSGGDAADLLPKR
IIYVSCNPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESMAVFERD

Sequences:

>Translated_450_residues
MPVAGPLDIVSLDGEARGIGRLTNEDGTPGKVVFVEGALPGERVMYRSHRVKPSYEQAGVVNILRQSASRVTPQCKFFGV
CGGCSMQHLDSRAQVAIKQRVLEDDLWHLAKLRPDVVFRPIAGPDWGYRYRARLTVRHVAAKGGVLVGFHERKSSYVADM
TSCEVLPPHVSALLVPLRELVGRLSIVQRMPQIELAVGQDVTALVLRNLEPITAEDEAELRAFADHHNIQFWLQPKGPDT
VYPFYPLDRTLTYTLPEFGITMPFKPTDFTQVNHQINRVLMSRALKLLDAQPGDRLLDLFCGIGNFTLPMATRAREVMGI
EGSEALTTRALANAQHNGLHTKTSFACRNLFEVSADDVAALGKFDRWLIDPPREGALAVSKALAELSQSGGDAADLLPKR
IIYVSCNPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESMAVFERD
>Mature_449_residues
PVAGPLDIVSLDGEARGIGRLTNEDGTPGKVVFVEGALPGERVMYRSHRVKPSYEQAGVVNILRQSASRVTPQCKFFGVC
GGCSMQHLDSRAQVAIKQRVLEDDLWHLAKLRPDVVFRPIAGPDWGYRYRARLTVRHVAAKGGVLVGFHERKSSYVADMT
SCEVLPPHVSALLVPLRELVGRLSIVQRMPQIELAVGQDVTALVLRNLEPITAEDEAELRAFADHHNIQFWLQPKGPDTV
YPFYPLDRTLTYTLPEFGITMPFKPTDFTQVNHQINRVLMSRALKLLDAQPGDRLLDLFCGIGNFTLPMATRAREVMGIE
GSEALTTRALANAQHNGLHTKTSFACRNLFEVSADDVAALGKFDRWLIDPPREGALAVSKALAELSQSGGDAADLLPKRI
IYVSCNPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESMAVFERD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=200, Percent_Identity=30, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI51173878, Length=200, Percent_Identity=30, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=37.4439461883408, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1787083, Length=401, Percent_Identity=24.4389027431421, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24666579, Length=212, Percent_Identity=29.2452830188679, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_RALPJ (B2U9U3)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898633.1
- ProteinModelPortal:   B2U9U3
- GeneID:   6289099
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1053
- HOGENOM:   HBG690163
- OMA:   VVFVEGA
- ProtClustDB:   PRK13168
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 49489; Mature: 49357

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 406-406 BINDING 271-271 BINDING 300-300 BINDING 305-305 BINDING 321-321 BINDING 349-349 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVAGPLDIVSLDGEARGIGRLTNEDGTPGKVVFVEGALPGERVMYRSHRVKPSYEQAGV
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCHHHHHH
VNILRQSASRVTPQCKFFGVCGGCSMQHLDSRAQVAIKQRVLEDDLWHLAKLRPDVVFRP
HHHHHHHHHHCCCCEEEEEECCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
IAGPDWGYRYRARLTVRHVAAKGGVLVGFHERKSSYVADMTSCEVLPPHVSALLVPLREL
CCCCCCCCEEEEEEEEEEHHCCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHH
VGRLSIVQRMPQIELAVGQDVTALVLRNLEPITAEDEAELRAFADHHNIQFWLQPKGPDT
HHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCC
VYPFYPLDRTLTYTLPEFGITMPFKPTDFTQVNHQINRVLMSRALKLLDAQPGDRLLDLF
CCCCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
CGIGNFTLPMATRAREVMGIEGSEALTTRALANAQHNGLHTKTSFACRNLFEVSADDVAA
HCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHH
LGKFDRWLIDPPREGALAVSKALAELSQSGGDAADLLPKRIIYVSCNPATLARDAGLLVH
HCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCHHHHHCCCEEEE
EAGYRLAGAGVVNMFPHTSHVESMAVFERD
CCCCEEECCCEEECCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
PVAGPLDIVSLDGEARGIGRLTNEDGTPGKVVFVEGALPGERVMYRSHRVKPSYEQAGV
CCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCHHHHHH
VNILRQSASRVTPQCKFFGVCGGCSMQHLDSRAQVAIKQRVLEDDLWHLAKLRPDVVFRP
HHHHHHHHHHCCCCEEEEEECCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
IAGPDWGYRYRARLTVRHVAAKGGVLVGFHERKSSYVADMTSCEVLPPHVSALLVPLREL
CCCCCCCCEEEEEEEEEEHHCCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHH
VGRLSIVQRMPQIELAVGQDVTALVLRNLEPITAEDEAELRAFADHHNIQFWLQPKGPDT
HHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCC
VYPFYPLDRTLTYTLPEFGITMPFKPTDFTQVNHQINRVLMSRALKLLDAQPGDRLLDLF
CCCCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
CGIGNFTLPMATRAREVMGIEGSEALTTRALANAQHNGLHTKTSFACRNLFEVSADDVAA
HCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHH
LGKFDRWLIDPPREGALAVSKALAELSQSGGDAADLLPKRIIYVSCNPATLARDAGLLVH
HCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCHHHHHCCCEEEE
EAGYRLAGAGVVNMFPHTSHVESMAVFERD
CCCCEEECCCEEECCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA