Click here to switch to the map view.

The map label for this gene is rpoS [H]

Identifier: 187928144

GI number: 187928144

Start: 1116794

End: 1117930

Strand: Direct

Name: rpoS [H]

Synonym: Rpic_1051

Alternate gene names: 187928144

Gene position: 1116794-1117930 (Clockwise)

Preceding gene: 187928143

Following gene: 187928146

Centisome position: 28.33

GC content: 62.01

Gene sequence:

>1137_bases
ATGCCGCGCCAGAAAGCCGCCACGCCCGTCACCCCAGCCGATGACAACATCGACGCTGCTGTAGAGGGCCAGCGCACCCC
AAACGGGCGCGCTGGGCGGCGTGCGCGCGGTGCGGCGCAGCCTCCTGCTGAAGAGATCGAAGTCGTCGATGCGCTTGTCG
ACGACCTCCCTGTTGCCGATTCCGCCGATCTGAACGACACGTCCGACAGTGCTGACGAGTCGGACGAAGAAGAGGAGGAG
GGCGAAGAGGCCGAGGAGGCTGCACCCGAGGAAGATTTCCGCAAGGTGCTGCAGGCCGAGCTGGCCGCCGACACTGTTCA
GCACTATCTCAATCGCATCAGCATCAAGCCGCTCCTGACGCCAGCGGAAGAGCTGCATTTCTCGACGCTGGCCAAGGCGG
GCGAATTTGCCGCGCGTCAGGTCATGATCGAGCGCAACCTCCGCCTGGTCGTGAGTATCGCCAAAGGTTATCTGAACCGC
GGTGTGCCGCTGCTCGATCTGATCGAGGAGGGCAACCTGGGTCTGATGCACGCGATCGAAAAGTTCGATCCGGAACGCGG
GTTCCGCTTCTCGACGTATGCAACGTGGTGGATCCGCCAAAGCATCGAGCGCGCGATCATGAATCAGGCGCGCACCGTCC
GTTTGCCGGTGCATGTGATTCGCGAGCTCAATCAGGTGCTGCGCGCTAAGCGCCATCTGGAAAAGAGCGGCGTGGATGGC
CGAGACGCGAGCCTTGAAGACATCGCCCACCTGCTTGGCAAGACCACGGAAGAAGTGCAGGACGTGCTCTCGCTCAACGA
GCACACGACTTCGCTGGATACGCCGTTTGATCTTGATCCGGGCACGAGCCTGCTCGACTTCCTATCGGACGAGCATGGCG
CATCGCCGGATCAGGAAGTGGCCCATCGCGAGCTGTCGCAGTTGATGAAGTCGTGGCTCGCACGCTTGTCCGACAAGCAT
CGCTATGTGGTCGAGCGCCGCTTTGGCCTCAACTACATCGAGCCTGCAACGCTGGAAGAACTGGCCGCCGAGATGGGTTT
GACGCGCGAACGCGTGCGGCAGATCCAGCAGGAGGCGCTGGTCAAGCTAAAGCGTCATTTCGCCTCTCAGGGCGTACGCA
AAGACGCGGTGCTGTAA

Upstream 100 bases:

>100_bases
GCAGTACCGATGCGGATCGCGTGAAGCTTCATTTTGAAGTGCGCCGCAACGGTAAGCCGGTCGATCCGATGCGCTACTTG
CCGCCTCAATAGAGGAATGC

Downstream 100 bases:

>100_bases
GGCGCCGCTGTCCCTCTTTCGTTCCCCTTTTTCAGTCGTTCCGCAGCGCCAGCAGCATCGGTTGATGATCCGATGCCTGT
GTAGCGCTGTTGATTTCCAG

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPRQKAATPVTPADDNIDAAVEGQRTPNGRAGRRARGAAQPPAEEIEVVDALVDDLPVADSADLNDTSDSADESDEEEEE
GEEAEEAAPEEDFRKVLQAELAADTVQHYLNRISIKPLLTPAEELHFSTLAKAGEFAARQVMIERNLRLVVSIAKGYLNR
GVPLLDLIEEGNLGLMHAIEKFDPERGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKSGVDG
RDASLEDIAHLLGKTTEEVQDVLSLNEHTTSLDTPFDLDPGTSLLDFLSDEHGASPDQEVAHRELSQLMKSWLARLSDKH
RYVVERRFGLNYIEPATLEELAAEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL

Sequences:

>Translated_378_residues
MPRQKAATPVTPADDNIDAAVEGQRTPNGRAGRRARGAAQPPAEEIEVVDALVDDLPVADSADLNDTSDSADESDEEEEE
GEEAEEAAPEEDFRKVLQAELAADTVQHYLNRISIKPLLTPAEELHFSTLAKAGEFAARQVMIERNLRLVVSIAKGYLNR
GVPLLDLIEEGNLGLMHAIEKFDPERGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKSGVDG
RDASLEDIAHLLGKTTEEVQDVLSLNEHTTSLDTPFDLDPGTSLLDFLSDEHGASPDQEVAHRELSQLMKSWLARLSDKH
RYVVERRFGLNYIEPATLEELAAEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL
>Mature_377_residues
PRQKAATPVTPADDNIDAAVEGQRTPNGRAGRRARGAAQPPAEEIEVVDALVDDLPVADSADLNDTSDSADESDEEEEEG
EEAEEAAPEEDFRKVLQAELAADTVQHYLNRISIKPLLTPAEELHFSTLAKAGEFAARQVMIERNLRLVVSIAKGYLNRG
VPLLDLIEEGNLGLMHAIEKFDPERGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKSGVDGR
DASLEDIAHLLGKTTEEVQDVLSLNEHTTSLDTPFDLDPGTSLLDFLSDEHGASPDQEVAHRELSQLMKSWLARLSDKHR
YVVERRFGLNYIEPATLEELAAEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=275, Percent_Identity=53.0909090909091, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1789448, Length=245, Percent_Identity=40, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789871, Length=278, Percent_Identity=29.8561151079137, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 42326; Mature: 42195

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQKAATPVTPADDNIDAAVEGQRTPNGRAGRRARGAAQPPAEEIEVVDALVDDLPVAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
SADLNDTSDSADESDEEEEEGEEAEEAAPEEDFRKVLQAELAADTVQHYLNRISIKPLLT
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAEELHFSTLAKAGEFAARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHH
KFDPERGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKSGVDG
HHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
RDASLEDIAHLLGKTTEEVQDVLSLNEHTTSLDTPFDLDPGTSLLDFLSDEHGASPDQEV
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHH
AHRELSQLMKSWLARLSDKHRYVVERRFGLNYIEPATLEELAAEMGLTRERVRQIQQEAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
VKLKRHFASQGVRKDAVL
HHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
PRQKAATPVTPADDNIDAAVEGQRTPNGRAGRRARGAAQPPAEEIEVVDALVDDLPVAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
SADLNDTSDSADESDEEEEEGEEAEEAAPEEDFRKVLQAELAADTVQHYLNRISIKPLLT
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAEELHFSTLAKAGEFAARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHH
KFDPERGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKSGVDG
HHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
RDASLEDIAHLLGKTTEEVQDVLSLNEHTTSLDTPFDLDPGTSLLDFLSDEHGASPDQEV
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHH
AHRELSQLMKSWLARLSDKHRYVVERRFGLNYIEPATLEELAAEMGLTRERVRQIQQEAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
VKLKRHFASQGVRKDAVL
HHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]