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The map label for this gene is nemA [H]

Identifier: 187928139

GI number: 187928139

Start: 1111874

End: 1112980

Strand: Direct

Name: nemA [H]

Synonym: Rpic_1046

Alternate gene names: 187928139

Gene position: 1111874-1112980 (Clockwise)

Preceding gene: 187928138

Following gene: 187928141

Centisome position: 28.2

GC content: 64.5

Gene sequence:

>1107_bases
ATGACTCAACAACTGCTGTCCCCGGTTCAGACCGGCCGTACGACCCTCGCGAATCGTGTCGTCATGGCGCCGCTTACGCG
CTCGCGCGCCGGCCAGCCGGGTGATGTGCCGACCGAATTGAACGTGACCTATTACGCGCAGCGCGCAACCGCTGGTTTGA
TTGTCACGGAAGCCACGCAAATTTCCCGCCAGGGCCAGGGCTACGCCTGGACGCCGGGCATGTACACCGATGCACAGGAA
GCCGGCTGGAAGACCGTGGTGGATGCTGTGCACGCCAAGGGTGGCCGCATCTCGCAACAGCTTTGGCACGTGGGCCGCAT
CTCCAACACGCTGCTGCAGGAAAACGGTCAGGCTCCCGTCGCGCCGTCAGCCATCGTCGCCAAGGGCGCGCAGAGCTTTG
TCGTGCAGCCCGACGGCACGCCCGCCAATGTGCCGACCAGCGAGCCACGTGCCCTGGCCACGGAAGAAATCCCCGGTGTG
ATCGCGCAATACCGCCAGGCCGTGCTGCGCGCGCGCCGCGCCGGTTTCGATTTCGTTGAAATCCACGCCGCCAACGGTTA
TCTGCTGCACCAATTCCTGTCGACCAACAGCAACCAGCGCACCGACCAGTACGGCGGCTCTCTGGAGAACCGCGCCCGAC
TGATTCTCGAAGTCGTTGACACTGCCATCGACGAGATTGGTGCAGATCGCGTCGGCATTCGCCTGTCGCCGCACTTCGTC
GCGCATGACATTGCCGACGCACAAGCCGAAGAAAGCGCGTTGTACCTCGCCCGCGAGCTGACCAAGCGTGGCATCGCCTA
CCTGCACATCGCCGAGCCGGATTGGGCCGGCGGTCCCGAGTTGACCAACGATTTCCGTCGTCAACTGCGCGACGCCTTCA
AGGGCACGCTCATCGTGTGCGGCCAATACACGGCGGAAGAGGGCGAGCAAATGATTGCCTCCGGTCTGGCCGATGCCGTG
GCGTTCGGACGCCCGTTCATCGCCAACCCCGACCTGGTGGCGCGCTTCCGTGCCGGCGCATCGCTCAACAAGCCGGATCG
CGAAACGTTCTACGGCGGCCAGGAAAAGGGCTACACCGACTATCCGACGCTGGAGGAGGCGGCCTGA

Upstream 100 bases:

>100_bases
TTCCATTCTGAAACCAATTGCCTGCGAGGCACGTTCCTCGCTTTTTCTTTGATTCCGGTTCTAGATGACCGGTCGAATAG
TCCCTCTTCCAGGAGCTGAT

Downstream 100 bases:

>100_bases
TAAGTCGCTGCACCAAAGAAAAAGCCGCGCAGGTTGATCGCCTGCGCGGCTTTTTTTGTCTGGCGCCTAAGTCTTTAAGG
CTGGATCACCACCTTGCCCG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTQQLLSPVQTGRTTLANRVVMAPLTRSRAGQPGDVPTELNVTYYAQRATAGLIVTEATQISRQGQGYAWTPGMYTDAQE
AGWKTVVDAVHAKGGRISQQLWHVGRISNTLLQENGQAPVAPSAIVAKGAQSFVVQPDGTPANVPTSEPRALATEEIPGV
IAQYRQAVLRARRAGFDFVEIHAANGYLLHQFLSTNSNQRTDQYGGSLENRARLILEVVDTAIDEIGADRVGIRLSPHFV
AHDIADAQAEESALYLARELTKRGIAYLHIAEPDWAGGPELTNDFRRQLRDAFKGTLIVCGQYTAEEGEQMIASGLADAV
AFGRPFIANPDLVARFRAGASLNKPDRETFYGGQEKGYTDYPTLEEAA

Sequences:

>Translated_368_residues
MTQQLLSPVQTGRTTLANRVVMAPLTRSRAGQPGDVPTELNVTYYAQRATAGLIVTEATQISRQGQGYAWTPGMYTDAQE
AGWKTVVDAVHAKGGRISQQLWHVGRISNTLLQENGQAPVAPSAIVAKGAQSFVVQPDGTPANVPTSEPRALATEEIPGV
IAQYRQAVLRARRAGFDFVEIHAANGYLLHQFLSTNSNQRTDQYGGSLENRARLILEVVDTAIDEIGADRVGIRLSPHFV
AHDIADAQAEESALYLARELTKRGIAYLHIAEPDWAGGPELTNDFRRQLRDAFKGTLIVCGQYTAEEGEQMIASGLADAV
AFGRPFIANPDLVARFRAGASLNKPDRETFYGGQEKGYTDYPTLEEAA
>Mature_367_residues
TQQLLSPVQTGRTTLANRVVMAPLTRSRAGQPGDVPTELNVTYYAQRATAGLIVTEATQISRQGQGYAWTPGMYTDAQEA
GWKTVVDAVHAKGGRISQQLWHVGRISNTLLQENGQAPVAPSAIVAKGAQSFVVQPDGTPANVPTSEPRALATEEIPGVI
AQYRQAVLRARRAGFDFVEIHAANGYLLHQFLSTNSNQRTDQYGGSLENRARLILEVVDTAIDEIGADRVGIRLSPHFVA
HDIADAQAEESALYLARELTKRGIAYLHIAEPDWAGGPELTNDFRRQLRDAFKGTLIVCGQYTAEEGEQMIASGLADAVA
FGRPFIANPDLVARFRAGASLNKPDRETFYGGQEKGYTDYPTLEEAA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=364, Percent_Identity=56.8681318681319, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1789463, Length=349, Percent_Identity=29.2263610315186, Blast_Score=117, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=218, Percent_Identity=31.1926605504587, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=343, Percent_Identity=29.1545189504373, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=256, Percent_Identity=32.03125, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=214, Percent_Identity=28.9719626168224, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=344, Percent_Identity=27.6162790697674, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=211, Percent_Identity=30.8056872037915, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=234, Percent_Identity=24.3589743589744, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=390, Percent_Identity=40, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321973, Length=392, Percent_Identity=36.734693877551, Blast_Score=222, Evalue=9e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39934; Mature: 39803

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQLLSPVQTGRTTLANRVVMAPLTRSRAGQPGDVPTELNVTYYAQRATAGLIVTEATQ
CCHHHHHHHHHHHHHHHCCEEECCCHHHCCCCCCCCCCCCCEEEEECCCCCCEEEECHHH
ISRQGQGYAWTPGMYTDAQEAGWKTVVDAVHAKGGRISQQLWHVGRISNTLLQENGQAPV
HHHCCCCEEECCCCCCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
APSAIVAKGAQSFVVQPDGTPANVPTSEPRALATEEIPGVIAQYRQAVLRARRAGFDFVE
CCHHHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IHAANGYLLHQFLSTNSNQRTDQYGGSLENRARLILEVVDTAIDEIGADRVGIRLSPHFV
EECCCCHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHH
AHDIADAQAEESALYLARELTKRGIAYLHIAEPDWAGGPELTNDFRRQLRDAFKGTLIVC
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEE
GQYTAEEGEQMIASGLADAVAFGRPFIANPDLVARFRAGASLNKPDRETFYGGQEKGYTD
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCCCCCCCCC
YPTLEEAA
CCCHHCCC
>Mature Secondary Structure 
TQQLLSPVQTGRTTLANRVVMAPLTRSRAGQPGDVPTELNVTYYAQRATAGLIVTEATQ
CHHHHHHHHHHHHHHHCCEEECCCHHHCCCCCCCCCCCCCEEEEECCCCCCEEEECHHH
ISRQGQGYAWTPGMYTDAQEAGWKTVVDAVHAKGGRISQQLWHVGRISNTLLQENGQAPV
HHHCCCCEEECCCCCCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
APSAIVAKGAQSFVVQPDGTPANVPTSEPRALATEEIPGVIAQYRQAVLRARRAGFDFVE
CCHHHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IHAANGYLLHQFLSTNSNQRTDQYGGSLENRARLILEVVDTAIDEIGADRVGIRLSPHFV
EECCCCHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHH
AHDIADAQAEESALYLARELTKRGIAYLHIAEPDWAGGPELTNDFRRQLRDAFKGTLIVC
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEE
GQYTAEEGEQMIASGLADAVAFGRPFIANPDLVARFRAGASLNKPDRETFYGGQEKGYTD
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHCCCCCCCCCCC
YPTLEEAA
CCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]