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The map label for this gene is pyrG [H]

Identifier: 187928062

GI number: 187928062

Start: 1027190

End: 1028851

Strand: Direct

Name: pyrG [H]

Synonym: Rpic_0969

Alternate gene names: 187928062

Gene position: 1027190-1028851 (Clockwise)

Preceding gene: 187928061

Following gene: 187928063

Centisome position: 26.05

GC content: 61.97

Gene sequence:

>1662_bases
ATGACCAAGTTCGTATTCGTTACCGGTGGCGTAGTGTCCTCACTCGGCAAGGGCATCGCCGCCGCCTCGCTCGCGGCCAT
TCTTGAGTCGCGCGGTCTCAAAGTCACCCTCCTCAAACTCGATCCCTACATCAACGTCGACCCGGGCACCATGAGCCCGT
TCCAGCACGGCGAAGTGTTTGTGACCGAGGACGGCGCAGAAACCGACCTCGATCTTGGCCACTACGAGCGTTTCGTCTCG
GCCAAGATGCGCAAGGCGAACAACTTCACCACCGGCCAGATCTACGAATCCGTGATCCGCAAGGAGCGTCGCGGCGAGTA
TCTCGGCAAGACGGTGCAAGTCATTCCCCATATCACCAACGAAATCCAGGCGTTCATCGAGCGCGGTGCTGCGGCATCGC
ACGACGGTAAGGCCGATGTGGCAATCGTGGAAATCGGCGGTACGGTGGGCGATATCGAATCGCTGCCGTTCCTCGAGGCC
GCACGCCAGATGAGCCTGCGCCTGGGTCGCAACCAGGTGGCGTTCGCGCATCTGACGCTGGTGCCGTTCATCGCCAGCGC
CGGCGAACTGAAGACCAAGCCGACCCAGCATTCGGTGCAGAAGCTGCGCGAAATCGGTGTCCAGCCGACCGCGCTGCTGT
GCCGCGCTGACCGCCCGATTCCCGACGATGAGCGTGCGAAGATTTCGCTCTTCGCCAACATGCCGCAGGACGCGGTGATC
TCGGTGTGGGATGTCGACACCATTTACAAGATCCCGCAGATGCTCAACGAGCAGGGGCTGGATCGCATCATTTGCGAAGA
GCTGCGCATCGAAGCTCCGCCGGCGGACCTGTCGGTGTGGGCGCATATGGTGCACACGCTGGAAAACCCGCAGCACGAGA
TCACCATCGGCATGGTCGGCAAGTACGTTGACCTGACCGAGTCGTACAAGTCGCTGATCGAAGCGCTGCGCCACGCCGGC
CTGCACACTTCGACGCGCGTCAATATCGAGTACATCGATTCCGAAGAACTGGAATCGGGCCATACGCAGGTGCTGGATAC
GCTGGATGCGATTCTGGTGCCGGGCGGCTTTGGCAAGCGTGGTACGGAAGGCAAGATTCGCGCAATCCAATACGCTCGTG
AAAAGGGTGTGCCGTACCTGGGCATCTGCCTGGGGATGCAGTTGGCCGTGATCGAGTTTGCACGCCATCTGGCCGGCATG
AAGGACGCCAACAGCACGGAATTCAACGACGAGACCGAGCATCCGGTGGTCGCGCTCATCACCGAATGGCTGGATCGCGA
TGGCCGCGTCGAAAAGCGCTCGGCCGACTCCGACCTGGGCGGCACCATGCGCCTGGGCTCGCAGCGCGTGCCGGTGCAAT
CGGGTACCAAGGCAGCGGCGATCTACGGCGCGGAAGTCAACGAACGCCATCGTCACCGCTACGAGGTGAACAACCACTAC
GTGCCGCAGCTTGAAAAGGCCGGGATGATCATCTCCGCGCGTACGCCGTCGGAAAACCTGCCGGAAATGATGGAACTGCC
GGCGTCGATGCACCCGTGGTTCGTCGGCGTGCAGTTCCACCCGGAATTCACCTCGACGCCGCGTGACGGCCACCCGCTGT
TCAAAGCCTACGTGGAAGCCGCGCTGGCACATCAGCAGAACACGCAACGCGCTGCCGCCTGA

Upstream 100 bases:

>100_bases
GCCGCATAGGCAAGACCGCGCTGGGGCAAATTCCACCGTTCTGTCACGGATCGGAGAGGGGACCTTTGCTATAATCCGGC
TTTCCCCGGCAAGCTAGGTC

Downstream 100 bases:

>100_bases
GCGCCGGCACACGGGAACATCATGAAACTCTGCGATTTCGAAGTCGGCCTCGACAAACCGTTCTTCCTGATTGCCGGCAC
CTGCGTGATCGAATCCGAGC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVS
AKMRKANNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFIERGAAASHDGKADVAIVEIGGTVGDIESLPFLEA
ARQMSLRLGRNQVAFAHLTLVPFIASAGELKTKPTQHSVQKLREIGVQPTALLCRADRPIPDDERAKISLFANMPQDAVI
SVWDVDTIYKIPQMLNEQGLDRIICEELRIEAPPADLSVWAHMVHTLENPQHEITIGMVGKYVDLTESYKSLIEALRHAG
LHTSTRVNIEYIDSEELESGHTQVLDTLDAILVPGGFGKRGTEGKIRAIQYAREKGVPYLGICLGMQLAVIEFARHLAGM
KDANSTEFNDETEHPVVALITEWLDRDGRVEKRSADSDLGGTMRLGSQRVPVQSGTKAAAIYGAEVNERHRHRYEVNNHY
VPQLEKAGMIISARTPSENLPEMMELPASMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAHQQNTQRAAA

Sequences:

>Translated_553_residues
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVS
AKMRKANNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFIERGAAASHDGKADVAIVEIGGTVGDIESLPFLEA
ARQMSLRLGRNQVAFAHLTLVPFIASAGELKTKPTQHSVQKLREIGVQPTALLCRADRPIPDDERAKISLFANMPQDAVI
SVWDVDTIYKIPQMLNEQGLDRIICEELRIEAPPADLSVWAHMVHTLENPQHEITIGMVGKYVDLTESYKSLIEALRHAG
LHTSTRVNIEYIDSEELESGHTQVLDTLDAILVPGGFGKRGTEGKIRAIQYAREKGVPYLGICLGMQLAVIEFARHLAGM
KDANSTEFNDETEHPVVALITEWLDRDGRVEKRSADSDLGGTMRLGSQRVPVQSGTKAAAIYGAEVNERHRHRYEVNNHY
VPQLEKAGMIISARTPSENLPEMMELPASMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAHQQNTQRAAA
>Mature_552_residues
TKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVSA
KMRKANNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFIERGAAASHDGKADVAIVEIGGTVGDIESLPFLEAA
RQMSLRLGRNQVAFAHLTLVPFIASAGELKTKPTQHSVQKLREIGVQPTALLCRADRPIPDDERAKISLFANMPQDAVIS
VWDVDTIYKIPQMLNEQGLDRIICEELRIEAPPADLSVWAHMVHTLENPQHEITIGMVGKYVDLTESYKSLIEALRHAGL
HTSTRVNIEYIDSEELESGHTQVLDTLDAILVPGGFGKRGTEGKIRAIQYAREKGVPYLGICLGMQLAVIEFARHLAGMK
DANSTEFNDETEHPVVALITEWLDRDGRVEKRSADSDLGGTMRLGSQRVPVQSGTKAAAIYGAEVNERHRHRYEVNNHYV
PQLEKAGMIISARTPSENLPEMMELPASMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAHQQNTQRAAA

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=561, Percent_Identity=44.7415329768271, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=561, Percent_Identity=44.0285204991087, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI28559083, Length=561, Percent_Identity=44.0285204991087, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI221316689, Length=561, Percent_Identity=44.0285204991087, Blast_Score=469, Evalue=1e-132,
Organism=Escherichia coli, GI1789142, Length=546, Percent_Identity=61.3553113553114, Blast_Score=684, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=611, Percent_Identity=38.7888707037643, Blast_Score=416, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322563, Length=575, Percent_Identity=41.7391304347826, Blast_Score=440, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6319432, Length=577, Percent_Identity=41.2478336221837, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24664469, Length=572, Percent_Identity=44.7552447552448, Blast_Score=468, Evalue=1e-132,
Organism=Drosophila melanogaster, GI21357815, Length=517, Percent_Identity=43.5203094777563, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 61035; Mature: 60904

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFVSAKMRKANNFTTGQIYESVIRKERRGEYLGKTVQVIPHITN
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIQAFIERGAAASHDGKADVAIVEIGGTVGDIESLPFLEAARQMSLRLGRNQVAFAHLTL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHH
VPFIASAGELKTKPTQHSVQKLREIGVQPTALLCRADRPIPDDERAKISLFANMPQDAVI
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEE
SVWDVDTIYKIPQMLNEQGLDRIICEELRIEAPPADLSVWAHMVHTLENPQHEITIGMVG
EEECHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
KYVDLTESYKSLIEALRHAGLHTSTRVNIEYIDSEELESGHTQVLDTLDAILVPGGFGKR
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHCCHHHHHHHHHHHEECCCCCCC
GTEGKIRAIQYAREKGVPYLGICLGMQLAVIEFARHLAGMKDANSTEFNDETEHPVVALI
CCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHH
TEWLDRDGRVEKRSADSDLGGTMRLGSQRVPVQSGTKAAAIYGAEVNERHRHRYEVNNHY
HHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCC
VPQLEKAGMIISARTPSENLPEMMELPASMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEA
CCCHHHCCEEEEECCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
ALAHQQNTQRAAA
HHHHHHCHHHCCC
>Mature Secondary Structure 
TKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFVSAKMRKANNFTTGQIYESVIRKERRGEYLGKTVQVIPHITN
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIQAFIERGAAASHDGKADVAIVEIGGTVGDIESLPFLEAARQMSLRLGRNQVAFAHLTL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHH
VPFIASAGELKTKPTQHSVQKLREIGVQPTALLCRADRPIPDDERAKISLFANMPQDAVI
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEE
SVWDVDTIYKIPQMLNEQGLDRIICEELRIEAPPADLSVWAHMVHTLENPQHEITIGMVG
EEECHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
KYVDLTESYKSLIEALRHAGLHTSTRVNIEYIDSEELESGHTQVLDTLDAILVPGGFGKR
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHCCHHHHHHHHHHHEECCCCCCC
GTEGKIRAIQYAREKGVPYLGICLGMQLAVIEFARHLAGMKDANSTEFNDETEHPVVALI
CCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHH
TEWLDRDGRVEKRSADSDLGGTMRLGSQRVPVQSGTKAAAIYGAEVNERHRHRYEVNNHY
HHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCC
VPQLEKAGMIISARTPSENLPEMMELPASMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEA
CCCHHHCCEEEEECCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
ALAHQQNTQRAAA
HHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]