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The map label for this gene is gudD [H]

Identifier: 187928041

GI number: 187928041

Start: 1001178

End: 1002548

Strand: Direct

Name: gudD [H]

Synonym: Rpic_0946

Alternate gene names: 187928041

Gene position: 1001178-1002548 (Clockwise)

Preceding gene: 187928039

Following gene: 187928042

Centisome position: 25.39

GC content: 65.72

Gene sequence:

>1371_bases
ATGACATACGTGATGACCATGGATCACAAAACCACCGACTCCCCACTTCGCACGCCGCGCGTTACCCGCCTTCAAGTCAT
TCCCGTGGCGGGCCGCGACAGTATGTTGTTGAATCTGAGCGGCGCGCACGGCCCGTTCTTCACCCGCAATATCGTCCTCC
TGGAGGACTCCGAGGGTCATACCGGGGTGGGCGAGGTGCCGGGCGGCGAGTCCATCCGCAAGACCATCGAGGACGCGACG
CCGCTGGTTGTCGGACAACCGATCGGCGCGTACAACAACGTATTGAACAGCGTACGTCGCCAATTTGCCGATCGCGACGT
CGCCGGCCGGGGGTTGCAGACCTTCGACCTGCGCACCACCGTGCATGCGGTGACGGCGCTGGAAAGCGCGCTGCTCGACT
TGCTCGGCCAGCATCTGGGCGTGCCTGTGGCGGCGCTGCTGGGGCAGGGGCAACAGCGCGACAAGGTGCCGGTGCTCGGC
TACCTGTTCTTCGTGGGCGACCGCAAACGCACCGACCTGGACTACGCCGACGGCAGCGATGCCACGGACGACTGGACCCG
CGTGCGCCACGAAGAAGCGCTTACGCCGGAGGCGGTCGTGCGCCTCGCAAAGGCCGCCCATGCGCGCTATGGCTTCAAGG
ATTTCAAGCTCAAGGGCGGTGTGCTGAGCGGCGATGACGAAATGGTCGTCACGACTGCATTGGCCGAGGCCTTTCCCGAG
GCGCGCGTCACGCTGGACCCGAACGGCGCGTGGTCGCTGGCCGAGGCCATTCGCCTGTGCCGCGACAAGCACGACGTGCT
GGCCTACGCCGAAGACCCGTGCGGCGCCGAGGGCGGTTACTCGGGCCGCGAAGTGATGGCGGAATTCCGCCGCGCCACGG
GCCTGCCGACGGCCACCAACATGATCGCCACCGACTGGCGCCAGATGGGGCACGCCATTCAGTTGCACTCCGTCGACATT
CCGCTGGCCGATCCGCATTTCTGGACGATGCAGGGCTCGGTGCGCGTGGCGCAGATGTGCGACGACTGGGGCCTGACCTG
GGGTTCGCACTCCAACAATCACTTCGACATTTCATTGGCGATGTTTACGCACGTGGCCGCGGCTGCGCCGGGCAACATCA
CCGCCATCGACACGCACTGGATCTGGCAAGACGGACAGCGCCTGACGCGTGAACCGTTCCAGATCGTCGGCGGTGAGATT
GCCGTTCCGGCCGCCCCGGGCCTGGGCGTCACGGTCGACATGGAGCAACTGGAGAAGGCCCACGCCCTCTACAAGCAGCA
CGGCCTGGGCGCGCGCGACGACGCAATCGCCATGCGCCAATTGATTCCCAACTGGCAGTTCGACAACAAGCGCCCCTGCA
TGGTGCGTTGA

Upstream 100 bases:

>100_bases
ACTGGCGGTGGGCATGCGGTCTCCGGGAGGGGCGGGTTTACCAGGGGTGGTTCGGTCGGAATGTGTCAATTATAGTCGCA
TCCGTGTTGTACGACGACTG

Downstream 100 bases:

>100_bases
CTGTTGACTGCTACGGCGGAACCCGAGGCGGCGCACGTCGTAGCACGCCGCCTCGTAGATCGGTAACCAATAAAAAAATA
TCGGAGACAACACCGTGAAG

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTYVMTMDHKTTDSPLRTPRVTRLQVIPVAGRDSMLLNLSGAHGPFFTRNIVLLEDSEGHTGVGEVPGGESIRKTIEDAT
PLVVGQPIGAYNNVLNSVRRQFADRDVAGRGLQTFDLRTTVHAVTALESALLDLLGQHLGVPVAALLGQGQQRDKVPVLG
YLFFVGDRKRTDLDYADGSDATDDWTRVRHEEALTPEAVVRLAKAAHARYGFKDFKLKGGVLSGDDEMVVTTALAEAFPE
ARVTLDPNGAWSLAEAIRLCRDKHDVLAYAEDPCGAEGGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAIQLHSVDI
PLADPHFWTMQGSVRVAQMCDDWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQRLTREPFQIVGGEI
AVPAAPGLGVTVDMEQLEKAHALYKQHGLGARDDAIAMRQLIPNWQFDNKRPCMVR

Sequences:

>Translated_456_residues
MTYVMTMDHKTTDSPLRTPRVTRLQVIPVAGRDSMLLNLSGAHGPFFTRNIVLLEDSEGHTGVGEVPGGESIRKTIEDAT
PLVVGQPIGAYNNVLNSVRRQFADRDVAGRGLQTFDLRTTVHAVTALESALLDLLGQHLGVPVAALLGQGQQRDKVPVLG
YLFFVGDRKRTDLDYADGSDATDDWTRVRHEEALTPEAVVRLAKAAHARYGFKDFKLKGGVLSGDDEMVVTTALAEAFPE
ARVTLDPNGAWSLAEAIRLCRDKHDVLAYAEDPCGAEGGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAIQLHSVDI
PLADPHFWTMQGSVRVAQMCDDWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQRLTREPFQIVGGEI
AVPAAPGLGVTVDMEQLEKAHALYKQHGLGARDDAIAMRQLIPNWQFDNKRPCMVR
>Mature_455_residues
TYVMTMDHKTTDSPLRTPRVTRLQVIPVAGRDSMLLNLSGAHGPFFTRNIVLLEDSEGHTGVGEVPGGESIRKTIEDATP
LVVGQPIGAYNNVLNSVRRQFADRDVAGRGLQTFDLRTTVHAVTALESALLDLLGQHLGVPVAALLGQGQQRDKVPVLGY
LFFVGDRKRTDLDYADGSDATDDWTRVRHEEALTPEAVVRLAKAAHARYGFKDFKLKGGVLSGDDEMVVTTALAEAFPEA
RVTLDPNGAWSLAEAIRLCRDKHDVLAYAEDPCGAEGGYSGREVMAEFRRATGLPTATNMIATDWRQMGHAIQLHSVDIP
LADPHFWTMQGSVRVAQMCDDWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQRLTREPFQIVGGEIA
VPAAPGLGVTVDMEQLEKAHALYKQHGLGARDDAIAMRQLIPNWQFDNKRPCMVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=444, Percent_Identity=73.4234234234234, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=442, Percent_Identity=63.5746606334842, Blast_Score=559, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49985; Mature: 49854

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYVMTMDHKTTDSPLRTPRVTRLQVIPVAGRDSMLLNLSGAHGPFFTRNIVLLEDSEGH
CEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCEEEEEEEEECCCCC
TGVGEVPGGESIRKTIEDATPLVVGQPIGAYNNVLNSVRRQFADRDVAGRGLQTFDLRTT
CCCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHH
VHAVTALESALLDLLGQHLGVPVAALLGQGQQRDKVPVLGYLFFVGDRKRTDLDYADGSD
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
ATDDWTRVRHEEALTPEAVVRLAKAAHARYGFKDFKLKGGVLSGDDEMVVTTALAEAFPE
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCEECCCCCEEHHHHHHHHCCC
ARVTLDPNGAWSLAEAIRLCRDKHDVLAYAEDPCGAEGGYSGREVMAEFRRATGLPTATN
CEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
MIATDWRQMGHAIQLHSVDIPLADPHFWTMQGSVRVAQMCDDWGLTWGSHSNNHFDISLA
HHHHHHHHHCCEEEEEEECCCCCCCCEEEEECCEEEEEHHHHCCCCCCCCCCCEEEEEHH
MFTHVAAAAPGNITAIDTHWIWQDGQRLTREPFQIVGGEIAVPAAPGLGVTVDMEQLEKA
HHHHHHHCCCCCEEEEEEEEEECCCCHHHCCCHHHCCCEEEECCCCCCCEEECHHHHHHH
HALYKQHGLGARDDAIAMRQLIPNWQFDNKRPCMVR
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TYVMTMDHKTTDSPLRTPRVTRLQVIPVAGRDSMLLNLSGAHGPFFTRNIVLLEDSEGH
EEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCEEEEEEEEECCCCC
TGVGEVPGGESIRKTIEDATPLVVGQPIGAYNNVLNSVRRQFADRDVAGRGLQTFDLRTT
CCCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHH
VHAVTALESALLDLLGQHLGVPVAALLGQGQQRDKVPVLGYLFFVGDRKRTDLDYADGSD
HHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCC
ATDDWTRVRHEEALTPEAVVRLAKAAHARYGFKDFKLKGGVLSGDDEMVVTTALAEAFPE
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCEECCCCCEEHHHHHHHHCCC
ARVTLDPNGAWSLAEAIRLCRDKHDVLAYAEDPCGAEGGYSGREVMAEFRRATGLPTATN
CEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
MIATDWRQMGHAIQLHSVDIPLADPHFWTMQGSVRVAQMCDDWGLTWGSHSNNHFDISLA
HHHHHHHHHCCEEEEEEECCCCCCCCEEEEECCEEEEEHHHHCCCCCCCCCCCEEEEEHH
MFTHVAAAAPGNITAIDTHWIWQDGQRLTREPFQIVGGEIAVPAAPGLGVTVDMEQLEKA
HHHHHHHCCCCCEEEEEEEEEECCCCHHHCCCHHHCCCEEEECCCCCCCEEECHHHHHHH
HALYKQHGLGARDDAIAMRQLIPNWQFDNKRPCMVR
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]