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The map label for this gene is pdxJ

Identifier: 187928026

GI number: 187928026

Start: 984606

End: 985397

Strand: Direct

Name: pdxJ

Synonym: Rpic_0931

Alternate gene names: 187928026

Gene position: 984606-985397 (Clockwise)

Preceding gene: 187928025

Following gene: 187928027

Centisome position: 24.97

GC content: 66.79

Gene sequence:

>792_bases
ATGATCTTTCATGCCTCGCCGGGAATCATCGACCTGGGCATCAATATCGACCACGTCGCCACGCTGCGCAACGCGCGCGG
CACCACCTACCCGGACCCGATCGCCGCGGCGCTGTTGGCCGAAGAAGCCGGCGCCGATCTCATTACCCTGCACCTGCGCG
AAGATCGCCGCCACATCAAGGACGCCGATGTGCGCGCGCTGCGGCCGCAACTGCGCACCCGCATGAACCTGGAGTGCGCC
GTCACGCAGGAGATGCTCGACATCGCGTGCGATATCGGCCCCCAAGACGTCTGCCTCGTGCCCGAGCGCCGTCAGGAAGT
GACCACCGAAGGCGGCCTGGACGTGGTGGGCGGTTTTGCCAAGGTGCAAGCCGCTTGTAAGCAGCTGGCCGATGCGGGCA
TCCGCGTGTCGCTGTTCATCGATCCGGATGCGGCACAGATCGAGGCGGCCGCCGCTGCCGGTGCTCCCGTCATCGAACTG
CATACGGGCCGCTATGCCGAAGCGACCGACGATGCTGAGCTGCGGGCGGAACTCGTCCGCATCGAGACCGCGGTGGAAGA
GGGCATCCGCTGGGGCGTGCGTGTGAACGCTGGCCACGGCCTCCACTACACGAACGTGCAGCCGATCGCGGCCATCGCCG
GCATTCATGAACTCAACATCGGCCACGCCGTCGTTGCGCATGCGGTGTTTGCCGGGTGGCAGAACGCGGTGCGCGAGATG
AAGGCGATCATGGTTGCGGCTCGGCTGTCGTCCACGACCCCGGCGGCCGCACACCCGGGCGTGCCTGTATGA

Upstream 100 bases:

>100_bases
CCGCGCAGCAAAGCCGCAGCCTGATGCGTTTCCTGCTGCATTATCATTTGCATGGCACGCCGCTTTCGACGCGCCAGATC
CTGATCGACTTGCAGAAACT

Downstream 100 bases:

>100_bases
GCGGACGGGGCGCAGCCGCCTCGGTGTCCGGTGCGATTTACGGCATCGGCACCGACATCATCCAGATCGAGCGCGTCGAA
GGCGTGATGCAGCGGACCAA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MIFHASPGIIDLGINIDHVATLRNARGTTYPDPIAAALLAEEAGADLITLHLREDRRHIKDADVRALRPQLRTRMNLECA
VTQEMLDIACDIGPQDVCLVPERRQEVTTEGGLDVVGGFAKVQAACKQLADAGIRVSLFIDPDAAQIEAAAAAGAPVIEL
HTGRYAEATDDAELRAELVRIETAVEEGIRWGVRVNAGHGLHYTNVQPIAAIAGIHELNIGHAVVAHAVFAGWQNAVREM
KAIMVAARLSSTTPAAAHPGVPV

Sequences:

>Translated_263_residues
MIFHASPGIIDLGINIDHVATLRNARGTTYPDPIAAALLAEEAGADLITLHLREDRRHIKDADVRALRPQLRTRMNLECA
VTQEMLDIACDIGPQDVCLVPERRQEVTTEGGLDVVGGFAKVQAACKQLADAGIRVSLFIDPDAAQIEAAAAAGAPVIEL
HTGRYAEATDDAELRAELVRIETAVEEGIRWGVRVNAGHGLHYTNVQPIAAIAGIHELNIGHAVVAHAVFAGWQNAVREM
KAIMVAARLSSTTPAAAHPGVPV
>Mature_263_residues
MIFHASPGIIDLGINIDHVATLRNARGTTYPDPIAAALLAEEAGADLITLHLREDRRHIKDADVRALRPQLRTRMNLECA
VTQEMLDIACDIGPQDVCLVPERRQEVTTEGGLDVVGGFAKVQAACKQLADAGIRVSLFIDPDAAQIEAAAAAGAPVIEL
HTGRYAEATDDAELRAELVRIETAVEEGIRWGVRVNAGHGLHYTNVQPIAAIAGIHELNIGHAVVAHAVFAGWQNAVREM
KAIMVAARLSSTTPAAAHPGVPV

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=237, Percent_Identity=64.5569620253165, Blast_Score=299, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_RALPJ (B2U984)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898513.1
- ProteinModelPortal:   B2U984
- SMR:   B2U984
- GeneID:   6289498
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0931
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 28016; Mature: 28016

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: ACT_SITE 51-51 ACT_SITE 78-78 ACT_SITE 199-199 BINDING 15-15 BINDING 26-26 BINDING 53-53 BINDING 58-58 BINDING 108-108 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFHASPGIIDLGINIDHVATLRNARGTTYPDPIAAALLAEEAGADLITLHLREDRRHIK
CEEECCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHCC
DADVRALRPQLRTRMNLECAVTQEMLDIACDIGPQDVCLVPERRQEVTTEGGLDVVGGFA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHCCCCCHHHHHHHH
KVQAACKQLADAGIRVSLFIDPDAAQIEAAAAAGAPVIELHTGRYAEATDDAELRAELVR
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHH
IETAVEEGIRWGVRVNAGHGLHYTNVQPIAAIAGIHELNIGHAVVAHAVFAGWQNAVREM
HHHHHHHHCEECEEEECCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KAIMVAARLSSTTPAAAHPGVPV
HHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIFHASPGIIDLGINIDHVATLRNARGTTYPDPIAAALLAEEAGADLITLHLREDRRHIK
CEEECCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHCC
DADVRALRPQLRTRMNLECAVTQEMLDIACDIGPQDVCLVPERRQEVTTEGGLDVVGGFA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHCCCCCHHHHHHHH
KVQAACKQLADAGIRVSLFIDPDAAQIEAAAAAGAPVIELHTGRYAEATDDAELRAELVR
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHH
IETAVEEGIRWGVRVNAGHGLHYTNVQPIAAIAGIHELNIGHAVVAHAVFAGWQNAVREM
HHHHHHHHCEECEEEECCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KAIMVAARLSSTTPAAAHPGVPV
HHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA