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The map label for this gene is lysS

Identifier: 187927988

GI number: 187927988

Start: 945219

End: 946763

Strand: Reverse

Name: lysS

Synonym: Rpic_0893

Alternate gene names: 187927988

Gene position: 946763-945219 (Counterclockwise)

Preceding gene: 187927989

Following gene: 187927987

Centisome position: 24.01

GC content: 62.98

Gene sequence:

>1545_bases
ATGACCGAACAGAACAACCCGGCCGAATCGAACACCGCGGCCGCCGTGCCGGCGCAGGACGACAACAAGATCATCGCCGA
GCGCCGCGAGAAGCTGGCAGAGATCCGCCAGCAAGGCGTCGCGTTTCCCAACGATTTCCGCCCGACGCACCATGCCGGTG
ATCTGCAAGCCAAGTATCGCGAGCTGGAGCAGACCGCACTGGAAGCCGAACCGGTGCAGGTGGCCATTGCCGGCCGCATG
ATGCTCAAGCGCGTGATGGGCAAGGCAAGCTTTGCCACCGTGCAGGACGCCACCGACCGCATCCAGTTCTACATCAGCCG
CGACGCCGTGGGCGAAGACGTCTACGCCAGCTTCAAGAAATGGGATCTGGGCGATATCGTGGCCGCACGCGGCACGCTGA
TGAAGACGAAGACCGGCGAGTTGTCGGTGGCGGTGACGGAGCTGCGCCTGCTCTCGAAGAGCCTGCGTCCGCTACCGGGC
GACTACTACGGCCTGGCAGATCAGGAACAGAAGTACCGTCAGCGCTACGTCGATCTGATCGTCTCGAAGGAAACGCGCGA
CACGTTCCGTGCCCGCACCAAGGCCATGGCCTCGCTGCGCAACTTCATGGCCGGCAACGGTTTCATGGAGGTCGAGACGC
CGATGCTGCACCCGATTCCGGGCGGCGCGGCAGCCAAACCGTTCATCACGCACCACAACGCGCTGGACATGCAGATGTTC
CTGCGCATCGCGCCCGAGCTGTACCTGAAGCGCCTGATCGTCGGTGGCTTCGAGCGCGTGTACGAGATCAACCGCAACTT
CCGTAACGAGGGCGTGTCGCCGCGACACAACCCCGAGTTCACGATGATGGAGTTCTACGCGGCCTACACGGACTACCGCT
GGCTGATGGACTTCACCGAGCAGTTGATCCGCCAGGCTGCCATTGACGCTTCTGGCACCGCCACGCTGACCTACCAGGGC
CGCGAGCTGGATCTTGCCAAGCCGTTCCATCGCCTGACCATCTGCCAGGCGATCCAGAAGTACGCGCCGCAATACACCGA
CGCGCAACTAGCTGACGTCGACTTCCTGCGCGCCGAACTGAAGAAGTTCAAGATCGACACCAACGCGCCGCAATTCCTGA
ACGCCGGCGTGGGTACGCTGCAGCTCGTGCTGTTTGAAGAGACGGCCGAGTCGCAACTGTGGGAGCCGACCTTCATCATC
GACTACCCGGTCGAAGTGTCGCCGCTGGCACGTGGCTCCGACACGCTGCCGGGCATCACCGAGCGCTTCGAGTTGTTCAT
CACCGGCCGCGAAATCGCCAACGGTTTCTCCGAGCTGAATGATCCTGAAGACCAGGCCGAGCGCTTCCGCAAGCAGGTCG
AGCAAAAGGACGCTGGCGACGAAGAAGCCATGTTCTACGACGCCGACTATATCCGCGCGTTGGAATACGGCATGCCCCCG
ACCGGCGGCTGCGGCATCGGCATCGACCGTCTGGTGATGCTGCTCACCGACAGCCCGAACATCCGCGACGTGATCCTCTT
CCCGCACCTGCGCCGCGAAGACTGA

Upstream 100 bases:

>100_bases
CACGCGCATCGCCGCCTACCTCGTCCGCCCCGACTTTGGCGCGACCGAGCTAGACGACCTGCGCAACATCGCCTGATACG
ACATTCCTCTCATCACCATC

Downstream 100 bases:

>100_bases
TCGGGCGCGATCGATCGCCTTGGAAAGAACGCCCCTTCGGGGGCGTTTTTTGTTGCGCGCGCGGCCGCAAATCACGCGCC
CACCCCGCTATTTGTAACAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTEQNNPAESNTAAAVPAQDDNKIIAERREKLAEIRQQGVAFPNDFRPTHHAGDLQAKYRELEQTALEAEPVQVAIAGRM
MLKRVMGKASFATVQDATDRIQFYISRDAVGEDVYASFKKWDLGDIVAARGTLMKTKTGELSVAVTELRLLSKSLRPLPG
DYYGLADQEQKYRQRYVDLIVSKETRDTFRARTKAMASLRNFMAGNGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMF
LRIAPELYLKRLIVGGFERVYEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDASGTATLTYQG
RELDLAKPFHRLTICQAIQKYAPQYTDAQLADVDFLRAELKKFKIDTNAPQFLNAGVGTLQLVLFEETAESQLWEPTFII
DYPVEVSPLARGSDTLPGITERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMFYDADYIRALEYGMPP
TGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED

Sequences:

>Translated_514_residues
MTEQNNPAESNTAAAVPAQDDNKIIAERREKLAEIRQQGVAFPNDFRPTHHAGDLQAKYRELEQTALEAEPVQVAIAGRM
MLKRVMGKASFATVQDATDRIQFYISRDAVGEDVYASFKKWDLGDIVAARGTLMKTKTGELSVAVTELRLLSKSLRPLPG
DYYGLADQEQKYRQRYVDLIVSKETRDTFRARTKAMASLRNFMAGNGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMF
LRIAPELYLKRLIVGGFERVYEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDASGTATLTYQG
RELDLAKPFHRLTICQAIQKYAPQYTDAQLADVDFLRAELKKFKIDTNAPQFLNAGVGTLQLVLFEETAESQLWEPTFII
DYPVEVSPLARGSDTLPGITERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMFYDADYIRALEYGMPP
TGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED
>Mature_513_residues
TEQNNPAESNTAAAVPAQDDNKIIAERREKLAEIRQQGVAFPNDFRPTHHAGDLQAKYRELEQTALEAEPVQVAIAGRMM
LKRVMGKASFATVQDATDRIQFYISRDAVGEDVYASFKKWDLGDIVAARGTLMKTKTGELSVAVTELRLLSKSLRPLPGD
YYGLADQEQKYRQRYVDLIVSKETRDTFRARTKAMASLRNFMAGNGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMFL
RIAPELYLKRLIVGGFERVYEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDASGTATLTYQGR
ELDLAKPFHRLTICQAIQKYAPQYTDAQLADVDFLRAELKKFKIDTNAPQFLNAGVGTLQLVLFEETAESQLWEPTFIID
YPVEVSPLARGSDTLPGITERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMFYDADYIRALEYGMPPT
GGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=507, Percent_Identity=40.2366863905325, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI194272210, Length=507, Percent_Identity=40.2366863905325, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI40789249, Length=245, Percent_Identity=24.8979591836735, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1789256, Length=505, Percent_Identity=58.6138613861386, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1790571, Length=505, Percent_Identity=58.4158415841584, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=32.2085889570552, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1788173, Length=314, Percent_Identity=24.5222929936306, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=485, Percent_Identity=42.2680412371134, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535927, Length=485, Percent_Identity=42.2680412371134, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71994340, Length=485, Percent_Identity=42.2680412371134, Blast_Score=367, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32566633, Length=295, Percent_Identity=26.7796610169492, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=521, Percent_Identity=40.4990403071017, Blast_Score=362, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324256, Length=439, Percent_Identity=32.8018223234624, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24640849, Length=488, Percent_Identity=42.0081967213115, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640851, Length=488, Percent_Identity=42.0081967213115, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24584738, Length=361, Percent_Identity=24.3767313019391, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_RALPJ (B2U8U9)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898475.1
- ProteinModelPortal:   B2U8U9
- SMR:   B2U8U9
- GeneID:   6288371
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0893
- HOGENOM:   HBG631383
- OMA:   GHPQMMS
- ProtClustDB:   PRK00484
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 58302; Mature: 58171

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQNNPAESNTAAAVPAQDDNKIIAERREKLAEIRQQGVAFPNDFRPTHHAGDLQAKYR
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
ELEQTALEAEPVQVAIAGRMMLKRVMGKASFATVQDATDRIQFYISRDAVGEDVYASFKK
HHHHHHHCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHC
WDLGDIVAARGTLMKTKTGELSVAVTELRLLSKSLRPLPGDYYGLADQEQKYRQRYVDLI
CCHHHHHHHCCCEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
VSKETRDTFRARTKAMASLRNFMAGNGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMF
HCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHH
LRIAPELYLKRLIVGGFERVYEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QLIRQAAIDASGTATLTYQGRELDLAKPFHRLTICQAIQKYAPQYTDAQLADVDFLRAEL
HHHHHHHCCCCCCEEEEECCCEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KKFKIDTNAPQFLNAGVGTLQLVLFEETAESQLWEPTFIIDYPVEVSPLARGSDTLPGIT
HHHCCCCCCCHHHHCCCCCEEEEEEECHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHH
ERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMFYDADYIRALEYGMPP
HEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCC
TGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED
CCCCCCCHHHEEEEEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
TEQNNPAESNTAAAVPAQDDNKIIAERREKLAEIRQQGVAFPNDFRPTHHAGDLQAKYR
CCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
ELEQTALEAEPVQVAIAGRMMLKRVMGKASFATVQDATDRIQFYISRDAVGEDVYASFKK
HHHHHHHCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHC
WDLGDIVAARGTLMKTKTGELSVAVTELRLLSKSLRPLPGDYYGLADQEQKYRQRYVDLI
CCHHHHHHHCCCEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
VSKETRDTFRARTKAMASLRNFMAGNGFMEVETPMLHPIPGGAAAKPFITHHNALDMQMF
HCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHH
LRIAPELYLKRLIVGGFERVYEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QLIRQAAIDASGTATLTYQGRELDLAKPFHRLTICQAIQKYAPQYTDAQLADVDFLRAEL
HHHHHHHCCCCCCEEEEECCCEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KKFKIDTNAPQFLNAGVGTLQLVLFEETAESQLWEPTFIIDYPVEVSPLARGSDTLPGIT
HHHCCCCCCCHHHHCCCCCEEEEEEECHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHH
ERFELFITGREIANGFSELNDPEDQAERFRKQVEQKDAGDEEAMFYDADYIRALEYGMPP
HEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCC
TGGCGIGIDRLVMLLTDSPNIRDVILFPHLRRED
CCCCCCCHHHEEEEEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA