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The map label for this gene is yueD [H]

Identifier: 187927989

GI number: 187927989

Start: 946788

End: 947528

Strand: Reverse

Name: yueD [H]

Synonym: Rpic_0894

Alternate gene names: 187927989

Gene position: 947528-946788 (Counterclockwise)

Preceding gene: 187927996

Following gene: 187927988

Centisome position: 24.03

GC content: 70.85

Gene sequence:

>741_bases
ATGTCTGACACGTCCCTGTTCATCCTGACCGGTGCCTCGCGCGGCCTGGGCGCCGCGCTGGCCCAGGCGCTGATGCAACC
CGGTCGCCGCCTCATCTGCGTCGCACGCGGCGATAACGCCGAATTGCGCGCGCAGGCGGCAGCCGCCGGTGTGACGCTCG
ACTGGCACCAGGTCGACCTGTCCGACGCGCATGCCGCCGAGGCTTGGATGACCAAGACGCTTGCCGCCCTGCCCGCTTTT
GCCAACGTGACGCTGGTGCTCAACGCCGGCGCGGTCGAGCCGATCGGCACCATCGACACATTGCGCGCCGATACGCTGCT
GCCGCACCTGCAACTGAACCTGGCCGGCCCGATGGCGCTGACCGCTGCCCTGCTGCGCGGCACCTCCGGGTGGAACGCGC
AGCGCCGCGTGCTGGCGATCTCGTCGGGCGCTGCGCGCCGGCCGATCGCCGGCTGGGCGGCGTATTGCACCGGCAAGGCC
GGGCTGGACATGTTCGTGCGCGCCATCAATGCCGATGGCGACGCGAGCGTGCGCGCCGTGGCACTCGCCCCCGGCGTGAT
CGACACCGACATGCAACGCACCATCCGTGGCGCCGACTTTGCCGGCGTGCAGCGCTTCCGCGATCTGCACGCGCATGGCG
AGTTTGTCTCGCCGCAGGTCGCCGCCACGCGCATCGCCGCCTACCTCGTCCGCCCCGACTTTGGCGCGACCGAGCTAGAC
GACCTGCGCAACATCGCCTGA

Upstream 100 bases:

>100_bases
ATCTCCAACACGCAGAAGGTGCTGGACGGCGATCTGGACCCGTTTATTCAGGCGAGCCTGAAGCAGGGCGTCTGATCCGA
GCCAAACCAGAGCGACTGCC

Downstream 100 bases:

>100_bases
TACGACATTCCTCTCATCACCATCATGACCGAACAGAACAACCCGGCCGAATCGAACACCGCGGCCGCCGTGCCGGCGCA
GGACGACAACAAGATCATCG

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSDTSLFILTGASRGLGAALAQALMQPGRRLICVARGDNAELRAQAAAAGVTLDWHQVDLSDAHAAEAWMTKTLAALPAF
ANVTLVLNAGAVEPIGTIDTLRADTLLPHLQLNLAGPMALTAALLRGTSGWNAQRRVLAISSGAARRPIAGWAAYCTGKA
GLDMFVRAINADGDASVRAVALAPGVIDTDMQRTIRGADFAGVQRFRDLHAHGEFVSPQVAATRIAAYLVRPDFGATELD
DLRNIA

Sequences:

>Translated_246_residues
MSDTSLFILTGASRGLGAALAQALMQPGRRLICVARGDNAELRAQAAAAGVTLDWHQVDLSDAHAAEAWMTKTLAALPAF
ANVTLVLNAGAVEPIGTIDTLRADTLLPHLQLNLAGPMALTAALLRGTSGWNAQRRVLAISSGAARRPIAGWAAYCTGKA
GLDMFVRAINADGDASVRAVALAPGVIDTDMQRTIRGADFAGVQRFRDLHAHGEFVSPQVAATRIAAYLVRPDFGATELD
DLRNIA
>Mature_245_residues
SDTSLFILTGASRGLGAALAQALMQPGRRLICVARGDNAELRAQAAAAGVTLDWHQVDLSDAHAAEAWMTKTLAALPAFA
NVTLVLNAGAVEPIGTIDTLRADTLLPHLQLNLAGPMALTAALLRGTSGWNAQRRVLAISSGAARRPIAGWAAYCTGKAG
LDMFVRAINADGDASVRAVALAPGVIDTDMQRTIRGADFAGVQRFRDLHAHGEFVSPQVAATRIAAYLVRPDFGATELDD
LRNIA

Specific function: Reduces benzil stereospecifically to (S)-benzoin. Can also reduces 1-phenyl-1,2-propanedione, 1,4-naphthoquinone, 1-(4- methyl-phenyl)-2-phenyl-ethane-1,2-dione, 1-(4-fluoro-phenyl)-2- phenyl-ethane-1,2-dione, methyl benzoylformate, p- nitrobenzaldehyde i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4507185, Length=243, Percent_Identity=31.6872427983539, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI115534694, Length=190, Percent_Identity=28.9473684210526, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322227, Length=244, Percent_Identity=27.0491803278689, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322226, Length=231, Percent_Identity=25.5411255411255, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI23397609, Length=218, Percent_Identity=33.0275229357798, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 25774; Mature: 25643

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTSLFILTGASRGLGAALAQALMQPGRRLICVARGDNAELRAQAAAAGVTLDWHQVDL
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCEEEEEEECC
SDAHAAEAWMTKTLAALPAFANVTLVLNAGAVEPIGTIDTLRADTLLPHLQLNLAGPMAL
CCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHCCEEEEECCCHHHH
TAALLRGTSGWNAQRRVLAISSGAARRPIAGWAAYCTGKAGLDMFVRAINADGDASVRAV
HHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCEEEE
ALAPGVIDTDMQRTIRGADFAGVQRFRDLHAHGEFVSPQVAATRIAAYLVRPDFGATELD
EECCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCCCHH
DLRNIA
HHHCCC
>Mature Secondary Structure 
SDTSLFILTGASRGLGAALAQALMQPGRRLICVARGDNAELRAQAAAAGVTLDWHQVDL
CCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCEEEEEEECC
SDAHAAEAWMTKTLAALPAFANVTLVLNAGAVEPIGTIDTLRADTLLPHLQLNLAGPMAL
CCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHCCEEEEECCCHHHH
TAALLRGTSGWNAQRRVLAISSGAARRPIAGWAAYCTGKAGLDMFVRAINADGDASVRAV
HHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCEEEE
ALAPGVIDTDMQRTIRGADFAGVQRFRDLHAHGEFVSPQVAATRIAAYLVRPDFGATELD
EECCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCCCCCCHH
DLRNIA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11745140; 11796169 [H]