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The map label for this gene is purR [H]

Identifier: 187927974

GI number: 187927974

Start: 933052

End: 934092

Strand: Reverse

Name: purR [H]

Synonym: Rpic_0879

Alternate gene names: 187927974

Gene position: 934092-933052 (Counterclockwise)

Preceding gene: 187927975

Following gene: 187927973

Centisome position: 23.69

GC content: 67.82

Gene sequence:

>1041_bases
ATGGCAACCATCAAAGACGTGGCCGCACTGGCCGGAGTTTCGTTCACCACCGTCTCGCACGTCATCAACAACACACGACC
GGTCAACGCCGAGACGCGCAAGCGCGTGGAAGAGGCCATCCGGGCCACGCGCTATGTGCCCAGCGCCGTGGCGCGCTCGC
TCAAACACCGCACCACGCGCACCATCGGCGTGCTCGTGCCCACCGCCACCAACCCCTACTTTGCGGAGCTGGCGCGGGGC
ATTGAAGATGTGTGTGCCGCCGCCGGCTACAGCGTCATCCTCTGCAACTCCGACGACGACCCCCGCAAGCAGCGCGACTA
CCTGCGCGTGCTGATGGAAAAACGCGTTGACGGCATCATCGTCAGCAGCGCCGGGCCAGACACGGCGTTGGTCGAAGCGC
TCGGTGAATCCGCCCTGCCCATCGTGATGGTCGACCGCCCGACCGAAGGCATCCAGGCCGATCAGGTCCAAGTCGATCAC
GAAGAAGGCGCGTACATGGCGACCAAGCATCTGCTGGACCTCGGCCACCGCCGCATCGCCTGCATCAGTGGGCCTTCGGC
ATTGAGCGTGACGGCGGAGCGCCTGGCCGGTTTCCACCGCGCGCTGCGCGAGGCGGGCGTGCCCGAAGCCACCGCCCGCA
TTGCCGAAGGCGATTTCACCAGCCCCGGCGGCTACCGCGCCGCGCGCCAGCTGCTGACCGAAGGCGAGATGCCCACGGCC
ATCTTTGCCGGCAATGACCTGATGGGCATCGGTGCGCTGCGCGCCGCGGCGGAACTGGGCATTCCCGTGCCGAAGGCGCT
GTCCGTGATGGGTTTTGACGACATCGAACTGAGCCGCTACGTCTATCCCGCGCTGTCCACTGTCGGCCAATCGATCCGCC
AGTTGGGCGAGACCACCGCGCAGACGCTGCTCGAGCATCTCGGCGACAACGCCTTGCGCCATCCCGTGGAAGAGCCGCGT
GCACGCCGCATCGTGCTGCCGCCGCGCCTGTCTTTGCGCGAGTCGACCGCCGAGCCCGAGCGCCCCGCCCGCGCCGCCTG
A

Upstream 100 bases:

>100_bases
CATCATCACGGGCGCGGTCATCGTCGCAGCCGTGGTCATGGATACCTATCGCAGCAAGCGCAACCGCAGCAAACACCATT
AAGAGACCGAGGAGATCGTC

Downstream 100 bases:

>100_bases
TCCCGCATCCCTTTCACGCTTTCAACGTAGTCCCGCCATGGTGGCCAAGCGCCCTTCCGCCTCTTCCGCGCACCCCGCTG
CCGACGTGCTGATCGTCGGC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MATIKDVAALAGVSFTTVSHVINNTRPVNAETRKRVEEAIRATRYVPSAVARSLKHRTTRTIGVLVPTATNPYFAELARG
IEDVCAAAGYSVILCNSDDDPRKQRDYLRVLMEKRVDGIIVSSAGPDTALVEALGESALPIVMVDRPTEGIQADQVQVDH
EEGAYMATKHLLDLGHRRIACISGPSALSVTAERLAGFHRALREAGVPEATARIAEGDFTSPGGYRAARQLLTEGEMPTA
IFAGNDLMGIGALRAAAELGIPVPKALSVMGFDDIELSRYVYPALSTVGQSIRQLGETTAQTLLEHLGDNALRHPVEEPR
ARRIVLPPRLSLRESTAEPERPARAA

Sequences:

>Translated_346_residues
MATIKDVAALAGVSFTTVSHVINNTRPVNAETRKRVEEAIRATRYVPSAVARSLKHRTTRTIGVLVPTATNPYFAELARG
IEDVCAAAGYSVILCNSDDDPRKQRDYLRVLMEKRVDGIIVSSAGPDTALVEALGESALPIVMVDRPTEGIQADQVQVDH
EEGAYMATKHLLDLGHRRIACISGPSALSVTAERLAGFHRALREAGVPEATARIAEGDFTSPGGYRAARQLLTEGEMPTA
IFAGNDLMGIGALRAAAELGIPVPKALSVMGFDDIELSRYVYPALSTVGQSIRQLGETTAQTLLEHLGDNALRHPVEEPR
ARRIVLPPRLSLRESTAEPERPARAA
>Mature_345_residues
ATIKDVAALAGVSFTTVSHVINNTRPVNAETRKRVEEAIRATRYVPSAVARSLKHRTTRTIGVLVPTATNPYFAELARGI
EDVCAAAGYSVILCNSDDDPRKQRDYLRVLMEKRVDGIIVSSAGPDTALVEALGESALPIVMVDRPTEGIQADQVQVDHE
EGAYMATKHLLDLGHRRIACISGPSALSVTAERLAGFHRALREAGVPEATARIAEGDFTSPGGYRAARQLLTEGEMPTAI
FAGNDLMGIGALRAAAELGIPVPKALSVMGFDDIELSRYVYPALSTVGQSIRQLGETTAQTLLEHLGDNALRHPVEEPRA
RRIVLPPRLSLRESTAEPERPARAA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=347, Percent_Identity=42.6512968299712, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI1790194, Length=341, Percent_Identity=40.1759530791789, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=35.8306188925081, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789202, Length=288, Percent_Identity=34.7222222222222, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788474, Length=345, Percent_Identity=35.0724637681159, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1786540, Length=342, Percent_Identity=32.1637426900585, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789068, Length=294, Percent_Identity=32.312925170068, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787580, Length=309, Percent_Identity=31.0679611650485, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787906, Length=346, Percent_Identity=27.4566473988439, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI48994940, Length=303, Percent_Identity=28.0528052805281, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790715, Length=319, Percent_Identity=25.705329153605, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789456, Length=350, Percent_Identity=28.2857142857143, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786268, Length=310, Percent_Identity=25.4838709677419, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790689, Length=332, Percent_Identity=25.9036144578313, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790192, Length=218, Percent_Identity=25.2293577981651, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37226; Mature: 37095

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAALAGVSFTTVSHVINNTRPVNAETRKRVEEAIRATRYVPSAVARSLKHRTTR
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
TIGVLVPTATNPYFAELARGIEDVCAAAGYSVILCNSDDDPRKQRDYLRVLMEKRVDGII
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCEEE
VSSAGPDTALVEALGESALPIVMVDRPTEGIQADQVQVDHEEGAYMATKHLLDLGHRRIA
EECCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEE
CISGPSALSVTAERLAGFHRALREAGVPEATARIAEGDFTSPGGYRAARQLLTEGEMPTA
EECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEE
IFAGNDLMGIGALRAAAELGIPVPKALSVMGFDDIELSRYVYPALSTVGQSIRQLGETTA
EEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QTLLEHLGDNALRHPVEEPRARRIVLPPRLSLRESTAEPERPARAA
HHHHHHHCCHHHHCCCCCCCCCEEECCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVAALAGVSFTTVSHVINNTRPVNAETRKRVEEAIRATRYVPSAVARSLKHRTTR
CCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
TIGVLVPTATNPYFAELARGIEDVCAAAGYSVILCNSDDDPRKQRDYLRVLMEKRVDGII
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCEEE
VSSAGPDTALVEALGESALPIVMVDRPTEGIQADQVQVDHEEGAYMATKHLLDLGHRRIA
EECCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEE
CISGPSALSVTAERLAGFHRALREAGVPEATARIAEGDFTSPGGYRAARQLLTEGEMPTA
EECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEE
IFAGNDLMGIGALRAAAELGIPVPKALSVMGFDDIELSRYVYPALSTVGQSIRQLGETTA
EEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QTLLEHLGDNALRHPVEEPRARRIVLPPRLSLRESTAEPERPARAA
HHHHHHHCCHHHHCCCCCCCCCEEECCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA