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The map label for this gene is yjbJ [H]

Identifier: 187927925

GI number: 187927925

Start: 876143

End: 877033

Strand: Direct

Name: yjbJ [H]

Synonym: Rpic_0830

Alternate gene names: 187927925

Gene position: 876143-877033 (Clockwise)

Preceding gene: 187927924

Following gene: 187927926

Centisome position: 22.22

GC content: 65.66

Gene sequence:

>891_bases
ATGAAGCTTTTCGTACGTTTCGCATTCGCATTGACGGCGCTGGCCTTGTTGGCCAGCCAGCCGGCGCGCGCGGACGTCTA
CGGCTACATCGATGAAAACGGTCTGGCGCACTTCGCTGCCGAGCGGATAGACGATCGCTACGTGCTGTTCATGAAGGGCG
TGGCGGTGGCGGGCAAGGGCGACGACAAGGCCAAGGACGATAGCGCCATCGCCCTGCCGGCAACCGAGCTGCCCGACGCC
GACGCCTATCTCAACGCCAGCAAGCGCGATGCACTCGACAACGCTGCCGGCATGCGCCAGCGCCTGGTGCGCGCGGTGTT
GCAGCATCCGAACGTGCCGACGGTGGAACCGCTGATCCGCCAGGCCGCGAGCAAGCACGGTATCGACCCCGCGTTGGTGA
AAGCCGTTATTGCCGCCGAATCCGGTTTCAACCCGCAGGCCGTGTCGCCCAAGGGTGCAATCGGGCTGATGCAGGTGATT
CCGGACACCGGCGCGCGCTACGGCGTGACCGGCGACGCCCGCCGCACGGCCGCCCAGAAGCTGGCCGACCCGAAGACCAA
CATCACCACCGGCGTGCGTTACCTGAGCGATCTGCTGCGCATGTTCTCAGGCAACCTTGAGCTTGTGCTGGCCGCCTACA
ACGCGGGCGAGGGCGCCGTGCAGAAGCACGGCAACGACATTCCGCCCTACGCCGAGACGCAGAACTACGTGAAGACGGTG
CTGCAGTTCTATCGCTACTACAACCCGGTCGCCGCCGTGCCCGGATTCATGAATGTGAGCGCCAACGGCGTCATCGCCAC
GCGCAAGCCGGGCCGCACGGGCCGGCCCGCACGCATCGAACTCGTATTGGACCCGGCCACCGGCGTGGCCACGAACGTAC
CTCAACATTGA

Upstream 100 bases:

>100_bases
AACATGCTGATGGGCAAGGGCGAAGCCGGCGCGCGCCGCACGTGGCTGGAAGCGCGCGGCAACGAAGTCGAAGCCGATAT
CTGATCGCAAACCGACAACG

Downstream 100 bases:

>100_bases
TCCTGGCGGCGCCGCAACGTTGAAGCGCGCCGCTTTCGCTTGAGTCTCCATGGAACAACAAGACATCCTCTTCGACCCCA
GCGAGCCGGCCGAAGCGCTC

Product: lytic transglycosylase catalytic subunit

Products: N-Acetylmuramic Acid Residues; N-Acetylglucosamine Residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKLFVRFAFALTALALLASQPARADVYGYIDENGLAHFAAERIDDRYVLFMKGVAVAGKGDDKAKDDSAIALPATELPDA
DAYLNASKRDALDNAAGMRQRLVRAVLQHPNVPTVEPLIRQAASKHGIDPALVKAVIAAESGFNPQAVSPKGAIGLMQVI
PDTGARYGVTGDARRTAAQKLADPKTNITTGVRYLSDLLRMFSGNLELVLAAYNAGEGAVQKHGNDIPPYAETQNYVKTV
LQFYRYYNPVAAVPGFMNVSANGVIATRKPGRTGRPARIELVLDPATGVATNVPQH

Sequences:

>Translated_296_residues
MKLFVRFAFALTALALLASQPARADVYGYIDENGLAHFAAERIDDRYVLFMKGVAVAGKGDDKAKDDSAIALPATELPDA
DAYLNASKRDALDNAAGMRQRLVRAVLQHPNVPTVEPLIRQAASKHGIDPALVKAVIAAESGFNPQAVSPKGAIGLMQVI
PDTGARYGVTGDARRTAAQKLADPKTNITTGVRYLSDLLRMFSGNLELVLAAYNAGEGAVQKHGNDIPPYAETQNYVKTV
LQFYRYYNPVAAVPGFMNVSANGVIATRKPGRTGRPARIELVLDPATGVATNVPQH
>Mature_296_residues
MKLFVRFAFALTALALLASQPARADVYGYIDENGLAHFAAERIDDRYVLFMKGVAVAGKGDDKAKDDSAIALPATELPDA
DAYLNASKRDALDNAAGMRQRLVRAVLQHPNVPTVEPLIRQAASKHGIDPALVKAVIAAESGFNPQAVSPKGAIGLMQVI
PDTGARYGVTGDARRTAAQKLADPKTNITTGVRYLSDLLRMFSGNLELVLAAYNAGEGAVQKHGNDIPPYAETQNYVKTV
LQFYRYYNPVAAVPGFMNVSANGVIATRKPGRTGRPARIELVLDPATGVATNVPQH

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082191, Length=112, Percent_Identity=37.5, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082441, Length=165, Percent_Identity=32.7272727272727, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081855, Length=84, Percent_Identity=41.6666666666667, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 31638; Mature: 31638

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFVRFAFALTALALLASQPARADVYGYIDENGLAHFAAERIDDRYVLFMKGVAVAGKG
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECEEEECCC
DDKAKDDSAIALPATELPDADAYLNASKRDALDNAAGMRQRLVRAVLQHPNVPTVEPLIR
CCCCCCCCEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
QAASKHGIDPALVKAVIAAESGFNPQAVSPKGAIGLMQVIPDTGARYGVTGDARRTAAQK
HHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHH
LADPKTNITTGVRYLSDLLRMFSGNLELVLAAYNAGEGAVQKHGNDIPPYAETQNYVKTV
HCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHH
LQFYRYYNPVAAVPGFMNVSANGVIATRKPGRTGRPARIELVLDPATGVATNVPQH
HHHHHHHCCHHHCCCCEECCCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKLFVRFAFALTALALLASQPARADVYGYIDENGLAHFAAERIDDRYVLFMKGVAVAGKG
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECEEEECCC
DDKAKDDSAIALPATELPDADAYLNASKRDALDNAAGMRQRLVRAVLQHPNVPTVEPLIR
CCCCCCCCEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
QAASKHGIDPALVKAVIAAESGFNPQAVSPKGAIGLMQVIPDTGARYGVTGDARRTAAQK
HHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHH
LADPKTNITTGVRYLSDLLRMFSGNLELVLAAYNAGEGAVQKHGNDIPPYAETQNYVKTV
HCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHH
LQFYRYYNPVAAVPGFMNVSANGVIATRKPGRTGRPARIELVLDPATGVATNVPQH
HHHHHHHCCHHHCCCCEECCCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]