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The map label for this gene is thyA [H]

Identifier: 187927913

GI number: 187927913

Start: 861159

End: 861953

Strand: Reverse

Name: thyA [H]

Synonym: Rpic_0818

Alternate gene names: 187927913

Gene position: 861953-861159 (Counterclockwise)

Preceding gene: 187927915

Following gene: 187927912

Centisome position: 21.86

GC content: 60.5

Gene sequence:

>795_bases
ATGAAGCCTTATCTCGACCTGATGCGCCATGTCTACGAACATGGCACCGACAAAGCCGACCGCACCGGCACCGGCACGCG
TTCCGTGTTCGGCCACCAGATGCGCTTCAACCTGCAGGACGGCTTCCCGCTGGTGACCACCAAGAAGGTGCACATCAAGT
CCATCGTCTATGAACTGCTGTGGTTCCTCCAGGGCTCGACCAACGTGCGATGGCTGCAAGACAACGGCGTGACGATCTGG
GACGAATGGGCCGACGCCGACGGCGAACTTGGCCCCGTCTACGGCTCCCAATGGCGCTCGTGGCCGACGCCCAACGGCGA
ACATATCGACCAGATCGCCGAGCTGATTGCCCAGATCAAGCGCAACCCCGATTCGCGCCGGCTGATCGTGTCTGCCTGGA
ATGTGGCCGACATCCCGCGCATGAAGCTGCCGCCCTGCCATGCGTTCTTCCAGTTCTATGTGGCCGACGGCAAGCTGTCG
TGCCAGCTCTACCAGCGCAGCGCCGATATCTTCCTCGGCGTGCCGTTCAACATTGCCAGCTACGCGCTGCTCGTGCACAT
GGTTGCGCAACAGACTGGCCTCGATGTGGGCGATTTCGTCTGGACTGGCGGCGATTGCCACCTCTATAACAACCATTTCG
AACAGGTCCAGACGCAACTCGCGCGCGAACCGCTGCCGTTGCCGAAACTGATCATCAAGCGCAAGCCTGAAAGCATCTTC
GACTACCAGTACGAGGACTTTGAGATCCTCGGCTACCAGAGCCACCCCGCGATCAAAGCACCGGTGGCTGTGTAA

Upstream 100 bases:

>100_bases
GCCAGTTTCCAGACACTGCCGGTACAGTCGACACCGCACCGCTCAAGTTACAATGCGCCTCACCCGATTTCCGACGCCCC
GCCTCTTCGCCTGCGCTCGC

Downstream 100 bases:

>100_bases
CCGTATGACCATCCTGACGCTCATCGTCGCCCGCGCCCGCAATGGCGTGATTGGCCGCAACAACACCCTACCCTGGCGCC
TGCCCGAAGATCTCGCGCAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKPYLDLMRHVYEHGTDKADRTGTGTRSVFGHQMRFNLQDGFPLVTTKKVHIKSIVYELLWFLQGSTNVRWLQDNGVTIW
DEWADADGELGPVYGSQWRSWPTPNGEHIDQIAELIAQIKRNPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGKLS
CQLYQRSADIFLGVPFNIASYALLVHMVAQQTGLDVGDFVWTGGDCHLYNNHFEQVQTQLAREPLPLPKLIIKRKPESIF
DYQYEDFEILGYQSHPAIKAPVAV

Sequences:

>Translated_264_residues
MKPYLDLMRHVYEHGTDKADRTGTGTRSVFGHQMRFNLQDGFPLVTTKKVHIKSIVYELLWFLQGSTNVRWLQDNGVTIW
DEWADADGELGPVYGSQWRSWPTPNGEHIDQIAELIAQIKRNPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGKLS
CQLYQRSADIFLGVPFNIASYALLVHMVAQQTGLDVGDFVWTGGDCHLYNNHFEQVQTQLAREPLPLPKLIIKRKPESIF
DYQYEDFEILGYQSHPAIKAPVAV
>Mature_264_residues
MKPYLDLMRHVYEHGTDKADRTGTGTRSVFGHQMRFNLQDGFPLVTTKKVHIKSIVYELLWFLQGSTNVRWLQDNGVTIW
DEWADADGELGPVYGSQWRSWPTPNGEHIDQIAELIAQIKRNPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGKLS
CQLYQRSADIFLGVPFNIASYALLVHMVAQQTGLDVGDFVWTGGDCHLYNNHFEQVQTQLAREPLPLPKLIIKRKPESIF
DYQYEDFEILGYQSHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=52.6690391459075, Blast_Score=291, Evalue=4e-79,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=73.4848484848485, Blast_Score=429, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=46.0992907801418, Blast_Score=256, Evalue=8e-69,
Organism=Saccharomyces cerevisiae, GI6324648, Length=228, Percent_Identity=44.7368421052632, Blast_Score=188, Evalue=6e-49,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=52.2968197879859, Blast_Score=294, Evalue=4e-80,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30276; Mature: 30276

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYLDLMRHVYEHGTDKADRTGTGTRSVFGHQMRFNLQDGFPLVTTKKVHIKSIVYELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEECCCCCCEEEECHHHHHHHHHHHH
WFLQGSTNVRWLQDNGVTIWDEWADADGELGPVYGSQWRSWPTPNGEHIDQIAELIAQIK
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RNPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHEEEECCCEEEEEEEECCCCEEEECCHHHHH
YALLVHMVAQQTGLDVGDFVWTGGDCHLYNNHFEQVQTQLAREPLPLPKLIIKRKPESIF
HHHHHHHHHHHHCCCCHHEEEECCEEEEECHHHHHHHHHHHHCCCCCHHHHHHCCCCHHH
DYQYEDFEILGYQSHPAIKAPVAV
CCCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKPYLDLMRHVYEHGTDKADRTGTGTRSVFGHQMRFNLQDGFPLVTTKKVHIKSIVYELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEECCCCCCEEEECHHHHHHHHHHHH
WFLQGSTNVRWLQDNGVTIWDEWADADGELGPVYGSQWRSWPTPNGEHIDQIAELIAQIK
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RNPDSRRLIVSAWNVADIPRMKLPPCHAFFQFYVADGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCCCCCCHHHHHHEEEECCCEEEEEEEECCCCEEEECCHHHHH
YALLVHMVAQQTGLDVGDFVWTGGDCHLYNNHFEQVQTQLAREPLPLPKLIIKRKPESIF
HHHHHHHHHHHHCCCCHHEEEECCEEEEECHHHHHHHHHHHHCCCCCHHHHHHCCCCHHH
DYQYEDFEILGYQSHPAIKAPVAV
CCCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]