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The map label for this gene is dfrA [H]

Identifier: 187927912

GI number: 187927912

Start: 860651

End: 861154

Strand: Reverse

Name: dfrA [H]

Synonym: Rpic_0817

Alternate gene names: 187927912

Gene position: 861154-860651 (Counterclockwise)

Preceding gene: 187927913

Following gene: 187927910

Centisome position: 21.84

GC content: 63.1

Gene sequence:

>504_bases
ATGACCATCCTGACGCTCATCGTCGCCCGCGCCCGCAATGGCGTGATTGGCCGCAACAACACCCTACCCTGGCGCCTGCC
CGAAGATCTCGCGCACTTCAAGCGCACGACCATGGGCGCGCCGATCGTGATGGGCCGCAAAACGTACGATTCGATCGGCC
GCCCGCTGCCGGGCCGTCGCAACATTGTCGTCACGCGCAATGCCAAGCTCGAACTGCCCGGCTGCGAGATCGCGTATTCG
ATGGAAGACGCGCAGCGCCTGTGTGTGGGTGCCGAGCAGATCTTCCTGATCGGTGGCGCGCAGCTGTATGCGGATGCCTT
GCCGAGCGCGGATCGACTGATCGTGACGGAGATCGACGCCGATTTCGAAGGCGATGCGCACTTCCCCGCGCCCGACCCAA
AGGTCTGGCACGAAGTCTCCCGTGAGACGCACCACGCGGCAGCGCCCAACGATTTCGACTATGCGTTCGTTACCTATGAA
CGTCCGCCATCGAACGAGCAATAA

Upstream 100 bases:

>100_bases
CGCAAGCCTGAAAGCATCTTCGACTACCAGTACGAGGACTTTGAGATCCTCGGCTACCAGAGCCACCCCGCGATCAAAGC
ACCGGTGGCTGTGTAACCGT

Downstream 100 bases:

>100_bases
CCGCTGCAGGCAATAAAAAACGCGCTCAGTTTCGAGCGCGTTTTTTTATTTCCGCTGTGGCAATTTATTCGCCGGCAATT
GTCATCTGTTCGATCAGGAT

Product: Dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTILTLIVARARNGVIGRNNTLPWRLPEDLAHFKRTTMGAPIVMGRKTYDSIGRPLPGRRNIVVTRNAKLELPGCEIAYS
MEDAQRLCVGAEQIFLIGGAQLYADALPSADRLIVTEIDADFEGDAHFPAPDPKVWHEVSRETHHAAAPNDFDYAFVTYE
RPPSNEQ

Sequences:

>Translated_167_residues
MTILTLIVARARNGVIGRNNTLPWRLPEDLAHFKRTTMGAPIVMGRKTYDSIGRPLPGRRNIVVTRNAKLELPGCEIAYS
MEDAQRLCVGAEQIFLIGGAQLYADALPSADRLIVTEIDADFEGDAHFPAPDPKVWHEVSRETHHAAAPNDFDYAFVTYE
RPPSNEQ
>Mature_166_residues
TILTLIVARARNGVIGRNNTLPWRLPEDLAHFKRTTMGAPIVMGRKTYDSIGRPLPGRRNIVVTRNAKLELPGCEIAYSM
EDAQRLCVGAEQIFLIGGAQLYADALPSADRLIVTEIDADFEGDAHFPAPDPKVWHEVSRETHHAAAPNDFDYAFVTYER
PPSNEQ

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=188, Percent_Identity=31.9148936170213, Blast_Score=77, Evalue=9e-15,
Organism=Escherichia coli, GI1786233, Length=163, Percent_Identity=43.558282208589, Blast_Score=140, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17505899, Length=188, Percent_Identity=32.9787234042553, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI116008026, Length=153, Percent_Identity=35.9477124183007, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24647458, Length=153, Percent_Identity=35.9477124183007, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18597; Mature: 18466

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILTLIVARARNGVIGRNNTLPWRLPEDLAHFKRTTMGAPIVMGRKTYDSIGRPLPGRR
CHHHHEEHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCC
NIVVTRNAKLELPGCEIAYSMEDAQRLCVGAEQIFLIGGAQLYADALPSADRLIVTEIDA
CEEEECCCEEECCCCEEEECHHHHHHHHCCCCEEEEEECHHHHHHCCCCCCEEEEEEECC
DFEGDAHFPAPDPKVWHEVSRETHHAAAPNDFDYAFVTYERPPSNEQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
TILTLIVARARNGVIGRNNTLPWRLPEDLAHFKRTTMGAPIVMGRKTYDSIGRPLPGRR
HHHHEEHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCC
NIVVTRNAKLELPGCEIAYSMEDAQRLCVGAEQIFLIGGAQLYADALPSADRLIVTEIDA
CEEEECCCEEECCCCEEEECHHHHHHHHCCCCEEEEEECHHHHHHCCCCCCEEEEEEECC
DFEGDAHFPAPDPKVWHEVSRETHHAAAPNDFDYAFVTYERPPSNEQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]