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The map label for this gene is yneJ [H]

Identifier: 187927846

GI number: 187927846

Start: 790346

End: 791254

Strand: Reverse

Name: yneJ [H]

Synonym: Rpic_0750

Alternate gene names: 187927846

Gene position: 791254-790346 (Counterclockwise)

Preceding gene: 187927853

Following gene: 187927845

Centisome position: 20.07

GC content: 66.89

Gene sequence:

>909_bases
ATGGATCTGTCCTCGCTAGAGATCTTCCGGACGGTCGTGCGGGAAGGCGGCGTCACGCGGGCGGCCCAGCAGTTGAATCG
CGTGCAGTCGAATGTGACGACACGCATCCGCCAGCTCGAAGCGTCACTCGGCGTGCAGCTGTTCGTGCGCGAAAGCAAGC
GCATGGTGCTGACGCCCGCCGGACAGACCCTGCTGACCTACGCCGACGACATCCTCCGTCTGGCCGCCGAGGCCCGCCAG
GCCGTGCAGCCGAGCGAGCCGCACGGGCGCCTGCGCATCGCCTCGATGGAAAGCACGGCGGCCAGCCGCCTTCCTGCCCT
GCTGGCCACGCTGCATCAACGCTGGCCGCGCGTACAGCTCGAACTGGTGACGATGACCACGCGCCACTCTCTTCAGGCGC
TATCGAACTTTGAAGTCGATTGCGCGTTCGTTGCCGAAACGGCGTGCCTGAACAACACGCGCGCCGCCATGACCACGCTG
CCGGCATTTGCCGAAGAGCTGGTGCTGATTGCCCACCGCGCCCATCCACCGATCCGCCGCGCGGCGGACGTGCAGACTGC
CGGCATGCTCGCGTTCGAACCTGGCTGCGCCTATCGGCAATTGATCGAAGACTGGTTCGCCGCAGACGGCGTGGTGCCGT
CCCGCGTGCTGGAGCTTGGCTCGTACCACGCCATCATCGCGTGCGCGGCCGCCGGCATTGGTGTGGCGCTGGTGCCTCGC
TCGGTGCTCGAGCTATATACGAATGCGCCAGAAATCAGCATCCACACGCTAGGCAAGCCGGGCCGTGTCGACACGCTGCT
TGCCTGGCACCGCGACATGGCCGGACCCGCCCTGCAGGCGCTCGTGCAGGTGCTGAGCGAGCCCCAAGCAGCGGCAAGCA
AAACCGCGCGGGAAGCCATTGCCGCCTAA

Upstream 100 bases:

>100_bases
AAGGGCCCGGATGGGGAAATCGGCACTTCATCGTACGCAAGGCCGCTCATCCTGAAAAATGAATTATATTGAACGAACCC
ATTAGCATTTGAGATACATC

Downstream 100 bases:

>100_bases
ACTGAAAGCGGATGCTGGGACCGTGCCGCCGGTCTGATCGGGCAAAAGCGCGGCAATCACGGACGGAGGCGCGAATGCGA
CCGTCGTTGCGGATGCGGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDLSSLEIFRTVVREGGVTRAAQQLNRVQSNVTTRIRQLEASLGVQLFVRESKRMVLTPAGQTLLTYADDILRLAAEARQ
AVQPSEPHGRLRIASMESTAASRLPALLATLHQRWPRVQLELVTMTTRHSLQALSNFEVDCAFVAETACLNNTRAAMTTL
PAFAEELVLIAHRAHPPIRRAADVQTAGMLAFEPGCAYRQLIEDWFAADGVVPSRVLELGSYHAIIACAAAGIGVALVPR
SVLELYTNAPEISIHTLGKPGRVDTLLAWHRDMAGPALQALVQVLSEPQAAASKTAREAIAA

Sequences:

>Translated_302_residues
MDLSSLEIFRTVVREGGVTRAAQQLNRVQSNVTTRIRQLEASLGVQLFVRESKRMVLTPAGQTLLTYADDILRLAAEARQ
AVQPSEPHGRLRIASMESTAASRLPALLATLHQRWPRVQLELVTMTTRHSLQALSNFEVDCAFVAETACLNNTRAAMTTL
PAFAEELVLIAHRAHPPIRRAADVQTAGMLAFEPGCAYRQLIEDWFAADGVVPSRVLELGSYHAIIACAAAGIGVALVPR
SVLELYTNAPEISIHTLGKPGRVDTLLAWHRDMAGPALQALVQVLSEPQAAASKTAREAIAA
>Mature_302_residues
MDLSSLEIFRTVVREGGVTRAAQQLNRVQSNVTTRIRQLEASLGVQLFVRESKRMVLTPAGQTLLTYADDILRLAAEARQ
AVQPSEPHGRLRIASMESTAASRLPALLATLHQRWPRVQLELVTMTTRHSLQALSNFEVDCAFVAETACLNNTRAAMTTL
PAFAEELVLIAHRAHPPIRRAADVQTAGMLAFEPGCAYRQLIEDWFAADGVVPSRVLELGSYHAIIACAAAGIGVALVPR
SVLELYTNAPEISIHTLGKPGRVDTLLAWHRDMAGPALQALVQVLSEPQAAASKTAREAIAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=292, Percent_Identity=39.7260273972603, Blast_Score=224, Evalue=4e-60,
Organism=Escherichia coli, GI87082132, Length=303, Percent_Identity=30.6930693069307, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788481, Length=245, Percent_Identity=28.9795918367347, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788296, Length=246, Percent_Identity=28.8617886178862, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788887, Length=294, Percent_Identity=24.8299319727891, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790208, Length=259, Percent_Identity=28.957528957529, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788748, Length=262, Percent_Identity=25.1908396946565, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790262, Length=264, Percent_Identity=25.7575757575758, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=28.0487804878049, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693105, Length=245, Percent_Identity=22.8571428571429, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=26.9565217391304, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787949, Length=311, Percent_Identity=24.1157556270096, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994962, Length=113, Percent_Identity=38.0530973451327, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=27.1255060728745, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=26.1627906976744, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786401, Length=152, Percent_Identity=32.2368421052632, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=24.7967479674797, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789204, Length=297, Percent_Identity=25.5892255892256, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787879, Length=259, Percent_Identity=25.8687258687259, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788626, Length=140, Percent_Identity=31.4285714285714, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32736; Mature: 32736

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSSLEIFRTVVREGGVTRAAQQLNRVQSNVTTRIRQLEASLGVQLFVRESKRMVLTPA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCC
GQTLLTYADDILRLAAEARQAVQPSEPHGRLRIASMESTAASRLPALLATLHQRWPRVQL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEE
ELVTMTTRHSLQALSNFEVDCAFVAETACLNNTRAAMTTLPAFAEELVLIAHRAHPPIRR
EEEEHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
AADVQTAGMLAFEPGCAYRQLIEDWFAADGVVPSRVLELGSYHAIIACAAAGIGVALVPR
HHCCHHCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHH
SVLELYTNAPEISIHTLGKPGRVDTLLAWHRDMAGPALQALVQVLSEPQAAASKTAREAI
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHH
AA
CC
>Mature Secondary Structure
MDLSSLEIFRTVVREGGVTRAAQQLNRVQSNVTTRIRQLEASLGVQLFVRESKRMVLTPA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCC
GQTLLTYADDILRLAAEARQAVQPSEPHGRLRIASMESTAASRLPALLATLHQRWPRVQL
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEE
ELVTMTTRHSLQALSNFEVDCAFVAETACLNNTRAAMTTLPAFAEELVLIAHRAHPPIRR
EEEEHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
AADVQTAGMLAFEPGCAYRQLIEDWFAADGVVPSRVLELGSYHAIIACAAAGIGVALVPR
HHCCHHCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHH
SVLELYTNAPEISIHTLGKPGRVDTLLAWHRDMAGPALQALVQVLSEPQAAASKTAREAI
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHH
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]