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The map label for this gene is rhtB [C]

Identifier: 187927853

GI number: 187927853

Start: 795723

End: 796589

Strand: Reverse

Name: rhtB [C]

Synonym: Rpic_0757

Alternate gene names: 187927853

Gene position: 796589-795723 (Counterclockwise)

Preceding gene: 187927856

Following gene: 187927846

Centisome position: 20.2

GC content: 62.4

Gene sequence:

>867_bases
ATGACGCACGCATGGCGTGCCGTCACCGATCGTTCAGCATTCTGTAAGCCGTTCCTTCCTCGCAGCCCGCGCTCACCAAA
AAATGAAACAAGCGTCGACGCTGCAGTCGGCGTGTTGTCTACGTGGGACCGATACAGAATCGCGAAAATTTATGGATTTC
TGTACCTGTTGGCCGAATCGCGGCCAGTGCTAGCCTGCAAGCTCTTCGCCCTTTCCGCAGACAGCGATTTCGCCATGCCT
ACCCTCACCAATCTGCTCACCTTTGCCTTGGTTGCGCTCGGCATGGTGCTGACGCCGGGCCCGAACATGATCTACCTAAT
CTCGCGTTCGATCTGCCAGGGGCGCAACGCCGGGCTGGTGTCGCTGGCGGGCATCGCCACGGGCTTCGTTTTCTACATGC
TGTGCGCCGCATTCGGCATCACCGCCTTGCTGATGGCCGTGCCGTTTGCCTACGATGCGCTGCGCTTTGGCGGTGCGATC
TATCTGCTGTACCTCGCCTGGCAGGCGGTGCGGCCGGGCGGCCGGGCACCGTTCCAGGTGCGTGAACTACCGGTGGACAG
CCCGCGCAAGCTTTACACGATGGGCTTGCTGACCAGCCTGCTCAATCCGAAGGTCGCAGTGCTCTATCTGTCGCTGCTGC
CGCAGTTCATCGAGCCGGCGCACGGCAGCGTGCTGGTGCAGTCGGTCTGCCTGGGGCTCACGCAGATCTGCGTGAGCGTG
ACCGTCAATTCGATGATTGCCGCGACGGCGGGCAGCATCGCCGCGTTTCTCTCGCGCAAGCCGAGCTGGCTGTCGGTTCA
GCGCTACCTGATGGGCACCGTGCTGACGAGCCTGGCGCTGCGCATGGCGATGGAAGCGCGCAAGTAG

Upstream 100 bases:

>100_bases
GCAACGCCACGGACAGCACGGCGGCAAGAAGGAAATGCAGGCGCATGGGGACGGGGGAGTTGGGGAATAAAAGGATTTCG
GCATACCCTCAGCAACGCCG

Downstream 100 bases:

>100_bases
CCGCACGCAGTCGATCAAAAGACCTGCCGCCAAATGAAAACGGGGCGATCCAGCCAAACCGGATCGCCCCGTTTTCTTTG
AACGCGCTGCCAGATCAGCG

Product: Lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTHAWRAVTDRSAFCKPFLPRSPRSPKNETSVDAAVGVLSTWDRYRIAKIYGFLYLLAESRPVLACKLFALSADSDFAMP
TLTNLLTFALVALGMVLTPGPNMIYLISRSICQGRNAGLVSLAGIATGFVFYMLCAAFGITALLMAVPFAYDALRFGGAI
YLLYLAWQAVRPGGRAPFQVRELPVDSPRKLYTMGLLTSLLNPKVAVLYLSLLPQFIEPAHGSVLVQSVCLGLTQICVSV
TVNSMIAATAGSIAAFLSRKPSWLSVQRYLMGTVLTSLALRMAMEARK

Sequences:

>Translated_288_residues
MTHAWRAVTDRSAFCKPFLPRSPRSPKNETSVDAAVGVLSTWDRYRIAKIYGFLYLLAESRPVLACKLFALSADSDFAMP
TLTNLLTFALVALGMVLTPGPNMIYLISRSICQGRNAGLVSLAGIATGFVFYMLCAAFGITALLMAVPFAYDALRFGGAI
YLLYLAWQAVRPGGRAPFQVRELPVDSPRKLYTMGLLTSLLNPKVAVLYLSLLPQFIEPAHGSVLVQSVCLGLTQICVSV
TVNSMIAATAGSIAAFLSRKPSWLSVQRYLMGTVLTSLALRMAMEARK
>Mature_287_residues
THAWRAVTDRSAFCKPFLPRSPRSPKNETSVDAAVGVLSTWDRYRIAKIYGFLYLLAESRPVLACKLFALSADSDFAMPT
LTNLLTFALVALGMVLTPGPNMIYLISRSICQGRNAGLVSLAGIATGFVFYMLCAAFGITALLMAVPFAYDALRFGGAIY
LLYLAWQAVRPGGRAPFQVRELPVDSPRKLYTMGLLTSLLNPKVAVLYLSLLPQFIEPAHGSVLVQSVCLGLTQICVSVT
VNSMIAATAGSIAAFLSRKPSWLSVQRYLMGTVLTSLALRMAMEARK

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=179, Percent_Identity=29.608938547486, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788099, Length=209, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786522, Length=118, Percent_Identity=27.9661016949153, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 31287; Mature: 31155

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHAWRAVTDRSAFCKPFLPRSPRSPKNETSVDAAVGVLSTWDRYRIAKIYGFLYLLAES
CCCCHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPVLACKLFALSADSDFAMPTLTNLLTFALVALGMVLTPGPNMIYLISRSICQGRNAGLV
CCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCHH
SLAGIATGFVFYMLCAAFGITALLMAVPFAYDALRFGGAIYLLYLAWQAVRPGGRAPFQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
RELPVDSPRKLYTMGLLTSLLNPKVAVLYLSLLPQFIEPAHGSVLVQSVCLGLTQICVSV
ECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TVNSMIAATAGSIAAFLSRKPSWLSVQRYLMGTVLTSLALRMAMEARK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THAWRAVTDRSAFCKPFLPRSPRSPKNETSVDAAVGVLSTWDRYRIAKIYGFLYLLAES
CCCHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RPVLACKLFALSADSDFAMPTLTNLLTFALVALGMVLTPGPNMIYLISRSICQGRNAGLV
CCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCHH
SLAGIATGFVFYMLCAAFGITALLMAVPFAYDALRFGGAIYLLYLAWQAVRPGGRAPFQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
RELPVDSPRKLYTMGLLTSLLNPKVAVLYLSLLPQFIEPAHGSVLVQSVCLGLTQICVSV
ECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TVNSMIAATAGSIAAFLSRKPSWLSVQRYLMGTVLTSLALRMAMEARK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]