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The map label for this gene is tolB [H]

Identifier: 187927780

GI number: 187927780

Start: 722091

End: 723395

Strand: Direct

Name: tolB [H]

Synonym: Rpic_0684

Alternate gene names: 187927780

Gene position: 722091-723395 (Clockwise)

Preceding gene: 187927779

Following gene: 187927781

Centisome position: 18.32

GC content: 65.06

Gene sequence:

>1305_bases
ATGAACATGATGCGAACGATGTGGATCCCGATGTTCCGACGCGCTGCGCAGGCACTGCTGCTGACCGCAGCGTGCGCCGG
CGTGGCCCATGCCCAGCTCAATGTGGAGATCTCGGGCGTCGGCGCCAGCCAGTACCCGATCGCGATCGCCAACTTCCAGG
GCGAGGCGCAGGCCCCGCAAAACCTCTCGGCCATCGTCCGGGCAGACCTGGTGCGCAGCGGCCGCTTCTCGAACGTCGAT
GTTGCGGGCGCCGTGGTACCGGAGAGCCCCGCACCTGACCTGGGTGCCTGGAAAGCCAAGGGCGCCGCAGCATTCGTGGG
CGGCACCGTGACCCGTAACGGCGACCGCTACGAAATCCGCTTCCGCCTGTACGACACCGCCAAGGGCGAAAGCCTGGGCG
GTCTGTCGCTTACGCGCGGCCCCGGCCAACTGCGCCTGGCCGCACACGAAATTGCCGATTACATCTACCAGAAGCTGATT
GGCGAGCGCGGTGTTTTTGCAACGCGCCTGTCGTACGTGTCCAAGGTCGGCCGCCGCTTCCAGCTGCAGATTTCCGACTC
GGACGGCGCCAACCCGCAGATCGCATTGACGAGCAACGAGCCGATCATCTCGCCGGCGTGGTCGCCGGATGGCACCAAGG
TCGCGTATGTGTCGTTCGAGAGCAAGAAGCCGGTGGTGTATGTGCACGACCTGGTGGCAGGCCGCCGCACGGTCATCTCG
AACCAGAAGGGCAACAACTCTGCGCCGGCCTGGTCGCCGGACGGCCGTCATGTGGCGGTGTCGCTCTCGCGCGATGGCAA
TACGCAGGTCTACCTGGTCAACGCCGATGGCTCGGGCCTGCGCCGCCTGAGCCGCAGCAGCGCGATCGATACCGAGCCGT
CGTTCTCGCCGGATGGCCGCTCCATCTACTTCACCAGCGACCGCGGTGGCGCACCGCAGATCTATCGCATGTCGGTTGAC
GGCGAAGAGGCGGGCGGTGCGCAACGCGTGACGTTCAAGGGCAACTACAACACGAGCCCGCGCGTGTCGCCAGACGGCAA
GCTGCTGGCGTACATTTCGCGCGTGGGCGGTGCCTTCCGCCTGTACACGCAGGATTTGTCGAATGGCGATGTGAACGCGT
TGACGGACACCTCTAACGATGAATCGCCGAGCTTTGCGGCCAACGGCAAGTTCATCCTGTATGCCACGCGCGTTGGCGGC
AAATCGGTGTTGGCCGCTGTGTCCACCGACGGCCGTACGCGTCAGGTTCTTTCCCTCCAGGCCGGCGAAGTTCGTGAGCC
GGCGTGGGGTCCTTTCATGCAATAA

Upstream 100 bases:

>100_bases
GAGCGTCATCCTGACGTTCCGGCCGAAGGAATAACGGGTTGGCGCTCCTCAAAGTGGCGCTGGCCGGCGTACTCAGATTC
CGGATTTGACAGTAGGGAAG

Downstream 100 bases:

>100_bases
CGAAGCTGTTCCAACAGGAGAAATCGGACATGTCGAAGTCCCAAACCATGATCAAACTTGCAGCCATTGCCGCGCTGCTG
GCCCTGGGCGCTTGCAGCTC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MNMMRTMWIPMFRRAAQALLLTAACAGVAHAQLNVEISGVGASQYPIAIANFQGEAQAPQNLSAIVRADLVRSGRFSNVD
VAGAVVPESPAPDLGAWKAKGAAAFVGGTVTRNGDRYEIRFRLYDTAKGESLGGLSLTRGPGQLRLAAHEIADYIYQKLI
GERGVFATRLSYVSKVGRRFQLQISDSDGANPQIALTSNEPIISPAWSPDGTKVAYVSFESKKPVVYVHDLVAGRRTVIS
NQKGNNSAPAWSPDGRHVAVSLSRDGNTQVYLVNADGSGLRRLSRSSAIDTEPSFSPDGRSIYFTSDRGGAPQIYRMSVD
GEEAGGAQRVTFKGNYNTSPRVSPDGKLLAYISRVGGAFRLYTQDLSNGDVNALTDTSNDESPSFAANGKFILYATRVGG
KSVLAAVSTDGRTRQVLSLQAGEVREPAWGPFMQ

Sequences:

>Translated_434_residues
MNMMRTMWIPMFRRAAQALLLTAACAGVAHAQLNVEISGVGASQYPIAIANFQGEAQAPQNLSAIVRADLVRSGRFSNVD
VAGAVVPESPAPDLGAWKAKGAAAFVGGTVTRNGDRYEIRFRLYDTAKGESLGGLSLTRGPGQLRLAAHEIADYIYQKLI
GERGVFATRLSYVSKVGRRFQLQISDSDGANPQIALTSNEPIISPAWSPDGTKVAYVSFESKKPVVYVHDLVAGRRTVIS
NQKGNNSAPAWSPDGRHVAVSLSRDGNTQVYLVNADGSGLRRLSRSSAIDTEPSFSPDGRSIYFTSDRGGAPQIYRMSVD
GEEAGGAQRVTFKGNYNTSPRVSPDGKLLAYISRVGGAFRLYTQDLSNGDVNALTDTSNDESPSFAANGKFILYATRVGG
KSVLAAVSTDGRTRQVLSLQAGEVREPAWGPFMQ
>Mature_434_residues
MNMMRTMWIPMFRRAAQALLLTAACAGVAHAQLNVEISGVGASQYPIAIANFQGEAQAPQNLSAIVRADLVRSGRFSNVD
VAGAVVPESPAPDLGAWKAKGAAAFVGGTVTRNGDRYEIRFRLYDTAKGESLGGLSLTRGPGQLRLAAHEIADYIYQKLI
GERGVFATRLSYVSKVGRRFQLQISDSDGANPQIALTSNEPIISPAWSPDGTKVAYVSFESKKPVVYVHDLVAGRRTVIS
NQKGNNSAPAWSPDGRHVAVSLSRDGNTQVYLVNADGSGLRRLSRSSAIDTEPSFSPDGRSIYFTSDRGGAPQIYRMSVD
GEEAGGAQRVTFKGNYNTSPRVSPDGKLLAYISRVGGAFRLYTQDLSNGDVNALTDTSNDESPSFAANGKFILYATRVGG
KSVLAAVSTDGRTRQVLSLQAGEVREPAWGPFMQ

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=420, Percent_Identity=37.8571428571429, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 46431; Mature: 46431

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMMRTMWIPMFRRAAQALLLTAACAGVAHAQLNVEISGVGASQYPIAIANFQGEAQAPQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEEECCCCCCCEEEEECCCCCCCCC
NLSAIVRADLVRSGRFSNVDVAGAVVPESPAPDLGAWKAKGAAAFVGGTVTRNGDRYEIR
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCEEECCCCEEEEE
FRLYDTAKGESLGGLSLTRGPGQLRLAAHEIADYIYQKLIGERGVFATRLSYVSKVGRRF
EEEEECCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEE
QLQISDSDGANPQIALTSNEPIISPAWSPDGTKVAYVSFESKKPVVYVHDLVAGRRTVIS
EEEECCCCCCCCEEEEECCCCEECCCCCCCCCEEEEEEECCCCCEEEEEEHHCCCEEEEE
NQKGNNSAPAWSPDGRHVAVSLSRDGNTQVYLVNADGSGLRRLSRSSAIDTEPSFSPDGR
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCC
SIYFTSDRGGAPQIYRMSVDGEEAGGAQRVTFKGNYNTSPRVSPDGKLLAYISRVGGAFR
EEEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEE
LYTQDLSNGDVNALTDTSNDESPSFAANGKFILYATRVGGKSVLAAVSTDGRTRQVLSLQ
EEEECCCCCCCEEEECCCCCCCCCEECCCEEEEEEEECCCCEEEEEECCCCCCEEEEEEC
AGEVREPAWGPFMQ
CCCCCCCCCCCCCC
>Mature Secondary Structure
MNMMRTMWIPMFRRAAQALLLTAACAGVAHAQLNVEISGVGASQYPIAIANFQGEAQAPQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEEECCCCCCCEEEEECCCCCCCCC
NLSAIVRADLVRSGRFSNVDVAGAVVPESPAPDLGAWKAKGAAAFVGGTVTRNGDRYEIR
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCEEECCCCEEEEE
FRLYDTAKGESLGGLSLTRGPGQLRLAAHEIADYIYQKLIGERGVFATRLSYVSKVGRRF
EEEEECCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEE
QLQISDSDGANPQIALTSNEPIISPAWSPDGTKVAYVSFESKKPVVYVHDLVAGRRTVIS
EEEECCCCCCCCEEEEECCCCEECCCCCCCCCEEEEEEECCCCCEEEEEEHHCCCEEEEE
NQKGNNSAPAWSPDGRHVAVSLSRDGNTQVYLVNADGSGLRRLSRSSAIDTEPSFSPDGR
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCC
SIYFTSDRGGAPQIYRMSVDGEEAGGAQRVTFKGNYNTSPRVSPDGKLLAYISRVGGAFR
EEEEECCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCEEE
LYTQDLSNGDVNALTDTSNDESPSFAANGKFILYATRVGGKSVLAAVSTDGRTRQVLSLQ
EEEECCCCCCCEEEECCCCCCCCCEECCCEEEEEEEECCCCEEEEEECCCCCCEEEEEEC
AGEVREPAWGPFMQ
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]