BacMap

Definition	Ralstonia pickettii 12J chromosome chromosome 1, complete sequence.
Accession	NC_010682
Length	3,942,557

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The map label for this gene is ydfG [H]

Identifier: 187927775

GI number: 187927775

Start: 718596

End: 719348

Strand: Direct

Name: ydfG [H]

Synonym: Rpic_0679

Alternate gene names: 187927775

Gene position: 718596-719348 (Clockwise)

Preceding gene: 187927772

Following gene: 187927776

Centisome position: 18.23

GC content: 63.75

Gene sequence:

>753_bases
ATGATCGTATTCGTGACCGGCGCAACCGCCGGGTTCGGCGCGGCCATCGCGCGGCGTTTCGTACAAGAAGGCCATCGTGT
GATCGCGGCAGGGCGCCGCCAGGACCGTCTTGATGCGCTCAAGGCCGAGTTGGGCGACGCGGTGCTGCCGTTCGTGCTGG
ATGTGCTGGACGTCGATGCCGTTGCTGCCTTGCCGGGCACCCTGCCCGAGGGCTGGCGCGAAGTCGACGTGCTCGTCAAC
AACGCCGGTCTGGCTCTCGGCCTTGAACCTGCCCAGCGCGCTGACCTGACCGATTGGGACCGCATGATCGGCACCAACTG
CTCCGGGCTGGTTCATATGACGCGTGCGTTACTTCCGGCCATGGTCGAGCGCAATCGCGGACACATCATCAATATCGGCT
CGATTGCGGGGACGTACCCATACCCGGGCGGCAACGTGTACGGCGCCACCAAGGCGTTCGTCCGCCAGTTCTCGTTGAAC
TTGCGCGCGGACTTGACTGGTACCCGCGTCCGCGTATCGAACGTTGAGCCAGGTCTTTGCTCCGGCACCGAGTTTTCCAA
CGTCCGTTTCAAGGGGGACGATGCCCGCGTAGAGAAGGTCTACGAAGGCACGGAAGCGCTGACGGCAGACGATATTGCCG
AGGCGGTCTACTGGATGGCGGCGCTTCCCGCGCACTTCAATGTCAACGCGATGGAGCTGATGCCCGTTTGCCAGTCATTC
TCGGCCACCAGCGTGACGCGAGGCATGGCCTGA

Upstream 100 bases:

>100_bases
GCAAGAAGCGGGCGTGCAGACACGCACATTGCGGTGCAATAAGGCACAAGGCTTCAGGTAAACTCTGATCTTTCGCATTT
TCAGGTCCGGAGACACTTTC

Downstream 100 bases:

>100_bases
ATTCGAGCGCAAGCCCTCGGCCAGTCGGTGGATGTCTGGCCGTCACTTGCGTCTTGGTAAAATTACTCGATGGCTTCTTT
TGACTGGAACCTGCGCGTGT

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MIVFVTGATAGFGAAIARRFVQEGHRVIAAGRRQDRLDALKAELGDAVLPFVLDVLDVDAVAALPGTLPEGWREVDVLVN
NAGLALGLEPAQRADLTDWDRMIGTNCSGLVHMTRALLPAMVERNRGHIINIGSIAGTYPYPGGNVYGATKAFVRQFSLN
LRADLTGTRVRVSNVEPGLCSGTEFSNVRFKGDDARVEKVYEGTEALTADDIAEAVYWMAALPAHFNVNAMELMPVCQSF
SATSVTRGMA

Sequences:

>Translated_250_residues
MIVFVTGATAGFGAAIARRFVQEGHRVIAAGRRQDRLDALKAELGDAVLPFVLDVLDVDAVAALPGTLPEGWREVDVLVN
NAGLALGLEPAQRADLTDWDRMIGTNCSGLVHMTRALLPAMVERNRGHIINIGSIAGTYPYPGGNVYGATKAFVRQFSLN
LRADLTGTRVRVSNVEPGLCSGTEFSNVRFKGDDARVEKVYEGTEALTADDIAEAVYWMAALPAHFNVNAMELMPVCQSF
SATSVTRGMA
>Mature_250_residues
MIVFVTGATAGFGAAIARRFVQEGHRVIAAGRRQDRLDALKAELGDAVLPFVLDVLDVDAVAALPGTLPEGWREVDVLVN
NAGLALGLEPAQRADLTDWDRMIGTNCSGLVHMTRALLPAMVERNRGHIINIGSIAGTYPYPGGNVYGATKAFVRQFSLN
LRADLTGTRVRVSNVEPGLCSGTEFSNVRFKGDDARVEKVYEGTEALTADDIAEAVYWMAALPAHFNVNAMELMPVCQSF
SATSVTRGMA

Specific function: Catalyzes the NADP-dependent oxidation of L-allo- threonine and L-serine [H]

COG id: COG4221

COG function: function code R; Short-chain alcohol dehydrogenase of unknown specificity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=30.6451612903226, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI150247226, Length=190, Percent_Identity=33.6842105263158, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI20149619, Length=191, Percent_Identity=31.9371727748691, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI7705855, Length=193, Percent_Identity=31.6062176165803, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI40548397, Length=179, Percent_Identity=35.195530726257, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI214010158, Length=179, Percent_Identity=35.195530726257, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI214010156, Length=179, Percent_Identity=35.195530726257, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI22758144, Length=179, Percent_Identity=35.195530726257, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI19743808, Length=178, Percent_Identity=33.7078651685393, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI7706318, Length=193, Percent_Identity=31.6062176165803, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI157739938, Length=184, Percent_Identity=29.3478260869565, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI22507405, Length=217, Percent_Identity=29.0322580645161, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI32483357, Length=204, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4557649, Length=178, Percent_Identity=29.2134831460674, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI5031737, Length=197, Percent_Identity=30.9644670050761, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI7657478, Length=180, Percent_Identity=31.1111111111111, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI33667109, Length=190, Percent_Identity=31.0526315789474, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI19923817, Length=187, Percent_Identity=26.2032085561497, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI44680136, Length=184, Percent_Identity=30.4347826086957, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI44680133, Length=184, Percent_Identity=30.4347826086957, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI17738292, Length=184, Percent_Identity=30.4347826086957, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI126723191, Length=198, Percent_Identity=29.7979797979798, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI50726952, Length=185, Percent_Identity=32.4324324324324, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI40254992, Length=176, Percent_Identity=30.1136363636364, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787820, Length=247, Percent_Identity=63.9676113360324, Blast_Score=339, Evalue=8e-95,
Organism=Escherichia coli, GI1787335, Length=228, Percent_Identity=31.140350877193, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082100, Length=182, Percent_Identity=32.967032967033, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786812, Length=201, Percent_Identity=27.8606965174129, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786701, Length=204, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI2367175, Length=238, Percent_Identity=26.4705882352941, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=240, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=247, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562906, Length=187, Percent_Identity=29.9465240641711, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=239, Percent_Identity=26.7782426778243, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=187, Percent_Identity=29.4117647058824, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=191, Percent_Identity=27.7486910994764, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=213, Percent_Identity=28.169014084507, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=184, Percent_Identity=29.3478260869565, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=238, Percent_Identity=26.890756302521, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562904, Length=213, Percent_Identity=30.5164319248826, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=186, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI193204405, Length=193, Percent_Identity=31.6062176165803, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=190, Percent_Identity=28.4210526315789, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=237, Percent_Identity=22.7848101265823, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=199, Percent_Identity=30.1507537688442, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=187, Percent_Identity=28.8770053475936, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=195, Percent_Identity=27.6923076923077, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=194, Percent_Identity=27.8350515463918, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=202, Percent_Identity=27.2277227722772, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=187, Percent_Identity=30.4812834224599, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI72000259, Length=192, Percent_Identity=27.6041666666667, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17565030, Length=224, Percent_Identity=28.125, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25152911, Length=251, Percent_Identity=26.6932270916335, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=255, Percent_Identity=33.7254901960784, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6322067, Length=178, Percent_Identity=30.3370786516854, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322227, Length=199, Percent_Identity=26.1306532663317, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116007236, Length=254, Percent_Identity=31.8897637795276, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21358495, Length=225, Percent_Identity=32.4444444444444, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI23397609, Length=235, Percent_Identity=31.9148936170213, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640442, Length=195, Percent_Identity=32.3076923076923, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI116007238, Length=195, Percent_Identity=33.8461538461538, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571526, Length=182, Percent_Identity=31.3186813186813, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357041, Length=183, Percent_Identity=31.1475409836066, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364494, Length=251, Percent_Identity=29.4820717131474, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI116007234, Length=253, Percent_Identity=30.8300395256917, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24644339, Length=240, Percent_Identity=29.1666666666667, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24641232, Length=255, Percent_Identity=29.0196078431373, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24663911, Length=252, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24643142, Length=176, Percent_Identity=31.25, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571387, Length=256, Percent_Identity=28.90625, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI161076933, Length=197, Percent_Identity=29.4416243654822, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24580925, Length=209, Percent_Identity=29.6650717703349, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24584748, Length=197, Percent_Identity=29.4416243654822, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648693, Length=253, Percent_Identity=26.8774703557312, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21355889, Length=253, Percent_Identity=26.8774703557312, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24641388, Length=256, Percent_Identity=27.34375, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28574402, Length=188, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584744, Length=188, Percent_Identity=27.6595744680851, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17737361, Length=191, Percent_Identity=31.413612565445, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21355631, Length=173, Percent_Identity=30.0578034682081, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007232, Length=184, Percent_Identity=33.1521739130435, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26809; Mature: 26809

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVFVTGATAGFGAAIARRFVQEGHRVIAAGRRQDRLDALKAELGDAVLPFVLDVLDVDA
CEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAALPGTLPEGWREVDVLVNNAGLALGLEPAQRADLTDWDRMIGTNCSGLVHMTRALLPA
HHHCCCCCCCCHHEEEEEEECCCEEEECCCHHHCCCCHHHHHHCCCCHHHHHHHHHHHHH
MVERNRGHIINIGSIAGTYPYPGGNVYGATKAFVRQFSLNLRADLTGTRVRVSNVEPGLC
HHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCEEEECCCCCCCC
SGTEFSNVRFKGDDARVEKVYEGTEALTADDIAEAVYWMAALPAHFNVNAMELMPVCQSF
CCCCCCCEEECCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
SATSVTRGMA
CHHHHHCCCC
>Mature Secondary Structure
MIVFVTGATAGFGAAIARRFVQEGHRVIAAGRRQDRLDALKAELGDAVLPFVLDVLDVDA
CEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAALPGTLPEGWREVDVLVNNAGLALGLEPAQRADLTDWDRMIGTNCSGLVHMTRALLPA
HHHCCCCCCCCHHEEEEEEECCCEEEECCCHHHCCCCHHHHHHCCCCHHHHHHHHHHHHH
MVERNRGHIINIGSIAGTYPYPGGNVYGATKAFVRQFSLNLRADLTGTRVRVSNVEPGLC
HHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCEEEECCCCCCCC
SGTEFSNVRFKGDDARVEKVYEGTEALTADDIAEAVYWMAALPAHFNVNAMELMPVCQSF
CCCCCCCEEECCCHHHHHHHHHCCHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
SATSVTRGMA
CHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]