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The map label for this gene is waaA [H]

Identifier: 187927739

GI number: 187927739

Start: 683000

End: 684316

Strand: Direct

Name: waaA [H]

Synonym: Rpic_0643

Alternate gene names: 187927739

Gene position: 683000-684316 (Clockwise)

Preceding gene: 187927738

Following gene: 187927740

Centisome position: 17.32

GC content: 67.43

Gene sequence:

>1317_bases
ATGCTGCGCGTTCTATATCGCTGGTTGTGGCGCATTGCGCTGCCGTTCGCATTGCTGCGCCTGTGGTGGCGCGGCCGCAA
GGAGCCCGGTTATCGACAGCATGTCGGCGAGCGGCTTGGCTTCTATCCGCCGCGCCCGAATCCTGACCGTCCGTTGCTGT
GGGTGCACGCCGTGTCCGTTGGCGAGACGCGCGCCGCGCAGCCGCTGATTGATGCCCTGTTGGCGCGCTTTCCGCACCAC
GCTGTCCTGCTGACGCACATGACGCCGACGGGCCGCCGCACGGGCGCTCAATTCGCGGCACAACGCAACCGCCGCGTCAT
CCAGGCGTATCTTCCGTACGATTTGCCGAGCGCAGTCGATCGGTTTCTGCGGCATTTCCAACCGCGCCTTGGCCTGCTGA
TGGAGACCGAGATCTGGCCGGTGCTGATCGAGCGCGCCTATACGGCCGGTGTGCCGATGGTGCTCGTCAACGGGCGCCTG
TCTGCGCGCAGTCACCGGCGTACCGCGCGCCTGGGTGACGCGGCGCGCCAGACGTACGCGCAGCTCGCCGCCGTGCTGGC
CCAGACGCCCGACGACGCCGATCGCTACCGCTCGCTCGGCGTGCCGCGCGTGCGGGTGACCGGCAACCTGAAGTTCGACA
TCACGCCGCATGTGGACCAGATCATGGCCGGACGTGCGTTGCACGACGCGCTACGTGGCCGATCGGTATGGGTGGCCGCC
AGCACGCGCGAGGGCGAAGAAGCGTTGCTGCTTGACGCGTGGCAGGCGCATCGCGCGCAGCATGTCGGGCGCCGGCATGC
GTTGCTGATTCTTGTGCCGCGTCACCCGCAGCGTTTTGATGAAGTGGCCCAGGCTGCCGAACGCGCGGGCTTGCGTGTCG
TGCGTCGCAGTGCGTTGTCGGTGTCTGCAGCAGGCGTGACGGAGTCCGATCAACTTGCCGACGCCGACGTTTTGCTCGGG
GACTCGATGGGCGAGATGGCACTGTATTACGCGGCTGGCGAAGTGGCGTTCATCGGCGGCAGCCTGCTTCCGCTCGGGGG
GCAGAATTTGATCGAGGCCTGCGCGGTCGGCACACCCGTGGTCATCGGCCCGCATACCTTTAACTTCGCGCAGGCGACTC
GCGATGCAGTTGCCGCGGGTGCGTGCGAGCAGGTAGGAGATGCCGCTGCGGCGATACGTGTGATCGACGCGTGGCTGTCC
GATGCCGATGCGCGCGAAGCCGCATCACGTGCCGCGCTGGCGTTTGCCGCCACACACGGCGGTGCGACGGCGCGCACGGT
GGAAGCGGTGGCATCCCTGGTCTTGCCAACGCTGTAA

Upstream 100 bases:

>100_bases
GGGGGTACTGGACGCCCAACGTGCACGACCTCGGCAGCGCCGAGGCCCATCCCACCAGCGCGCAGGCGCTCGATGCGCTG
CGTGCGCTGGGTGTGCTCTG

Downstream 100 bases:

>100_bases
CGCTGTGTACGTCCGGCACGAAACGTGCGAACTCATGGCGAGCCGGGCAGACTAATGACTCTTCGTGTAGTTTGCATTCC
GTCACCGTTCACGATCTGTT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MLRVLYRWLWRIALPFALLRLWWRGRKEPGYRQHVGERLGFYPPRPNPDRPLLWVHAVSVGETRAAQPLIDALLARFPHH
AVLLTHMTPTGRRTGAQFAAQRNRRVIQAYLPYDLPSAVDRFLRHFQPRLGLLMETEIWPVLIERAYTAGVPMVLVNGRL
SARSHRRTARLGDAARQTYAQLAAVLAQTPDDADRYRSLGVPRVRVTGNLKFDITPHVDQIMAGRALHDALRGRSVWVAA
STREGEEALLLDAWQAHRAQHVGRRHALLILVPRHPQRFDEVAQAAERAGLRVVRRSALSVSAAGVTESDQLADADVLLG
DSMGEMALYYAAGEVAFIGGSLLPLGGQNLIEACAVGTPVVIGPHTFNFAQATRDAVAAGACEQVGDAAAAIRVIDAWLS
DADAREAASRAALAFAATHGGATARTVEAVASLVLPTL

Sequences:

>Translated_438_residues
MLRVLYRWLWRIALPFALLRLWWRGRKEPGYRQHVGERLGFYPPRPNPDRPLLWVHAVSVGETRAAQPLIDALLARFPHH
AVLLTHMTPTGRRTGAQFAAQRNRRVIQAYLPYDLPSAVDRFLRHFQPRLGLLMETEIWPVLIERAYTAGVPMVLVNGRL
SARSHRRTARLGDAARQTYAQLAAVLAQTPDDADRYRSLGVPRVRVTGNLKFDITPHVDQIMAGRALHDALRGRSVWVAA
STREGEEALLLDAWQAHRAQHVGRRHALLILVPRHPQRFDEVAQAAERAGLRVVRRSALSVSAAGVTESDQLADADVLLG
DSMGEMALYYAAGEVAFIGGSLLPLGGQNLIEACAVGTPVVIGPHTFNFAQATRDAVAAGACEQVGDAAAAIRVIDAWLS
DADAREAASRAALAFAATHGGATARTVEAVASLVLPTL
>Mature_438_residues
MLRVLYRWLWRIALPFALLRLWWRGRKEPGYRQHVGERLGFYPPRPNPDRPLLWVHAVSVGETRAAQPLIDALLARFPHH
AVLLTHMTPTGRRTGAQFAAQRNRRVIQAYLPYDLPSAVDRFLRHFQPRLGLLMETEIWPVLIERAYTAGVPMVLVNGRL
SARSHRRTARLGDAARQTYAQLAAVLAQTPDDADRYRSLGVPRVRVTGNLKFDITPHVDQIMAGRALHDALRGRSVWVAA
STREGEEALLLDAWQAHRAQHVGRRHALLILVPRHPQRFDEVAQAAERAGLRVVRRSALSVSAAGVTESDQLADADVLLG
DSMGEMALYYAAGEVAFIGGSLLPLGGQNLIEACAVGTPVVIGPHTFNFAQATRDAVAAGACEQVGDAAAAIRVIDAWLS
DADAREAASRAALAFAATHGGATARTVEAVASLVLPTL

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=446, Percent_Identity=39.9103139013453, Blast_Score=281, Evalue=5e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47916; Mature: 47916

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVLYRWLWRIALPFALLRLWWRGRKEPGYRQHVGERLGFYPPRPNPDRPLLWVHAVSV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEEEC
GETRAAQPLIDALLARFPHHAVLLTHMTPTGRRTGAQFAAQRNRRVIQAYLPYDLPSAVD
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCHHHHH
RFLRHFQPRLGLLMETEIWPVLIERAYTAGVPMVLVNGRLSARSHRRTARLGDAARQTYA
HHHHHHCHHHCCEEECHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
QLAAVLAQTPDDADRYRSLGVPRVRVTGNLKFDITPHVDQIMAGRALHDALRGRSVWVAA
HHHHHHHCCCCHHHHHHHCCCCEEEEEECEEEECCCCHHHHHHHHHHHHHHCCCEEEEEE
STREGEEALLLDAWQAHRAQHVGRRHALLILVPRHPQRFDEVAQAAERAGLRVVRRSALS
CCCCCCCEEEHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
VSAAGVTESDQLADADVLLGDSMGEMALYYAAGEVAFIGGSLLPLGGQNLIEACAVGTPV
EEECCCCCCCCCCCCCEEECCCCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCCCE
VIGPHTFNFAQATRDAVAAGACEQVGDAAAAIRVIDAWLSDADAREAASRAALAFAATHG
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCC
GATARTVEAVASLVLPTL
CCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLRVLYRWLWRIALPFALLRLWWRGRKEPGYRQHVGERLGFYPPRPNPDRPLLWVHAVSV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEEEC
GETRAAQPLIDALLARFPHHAVLLTHMTPTGRRTGAQFAAQRNRRVIQAYLPYDLPSAVD
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCHHHHH
RFLRHFQPRLGLLMETEIWPVLIERAYTAGVPMVLVNGRLSARSHRRTARLGDAARQTYA
HHHHHHCHHHCCEEECHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
QLAAVLAQTPDDADRYRSLGVPRVRVTGNLKFDITPHVDQIMAGRALHDALRGRSVWVAA
HHHHHHHCCCCHHHHHHHCCCCEEEEEECEEEECCCCHHHHHHHHHHHHHHCCCEEEEEE
STREGEEALLLDAWQAHRAQHVGRRHALLILVPRHPQRFDEVAQAAERAGLRVVRRSALS
CCCCCCCEEEHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
VSAAGVTESDQLADADVLLGDSMGEMALYYAAGEVAFIGGSLLPLGGQNLIEACAVGTPV
EEECCCCCCCCCCCCCEEECCCCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHCCCCE
VIGPHTFNFAQATRDAVAAGACEQVGDAAAAIRVIDAWLSDADAREAASRAALAFAATHG
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEECCC
GATARTVEAVASLVLPTL
CCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]