Click here to switch to the map view.

The map label for this gene is pykA [H]

Identifier: 187927592

GI number: 187927592

Start: 524061

End: 525500

Strand: Direct

Name: pykA [H]

Synonym: Rpic_0492

Alternate gene names: 187927592

Gene position: 524061-525500 (Clockwise)

Preceding gene: 187927591

Following gene: 187927593

Centisome position: 13.29

GC content: 65.97

Gene sequence:

>1440_bases
ATGACCCGCGCTACCAAGATCGTCGCAACGCTCGGCCCCGCGTCCAGCACGCAGGAAGTCCTGACCCGCATGATCGCCGC
GGGGGTCGACGTGGTGCGGCTGAACTTCTCGCACGGCGCGGCGCAGGATCACATCGATCGTGCAAAGCTCGTGCGCGAAG
TGGCGCGCTCGGTGGGCCGCGAGGTGGCCATCATGGCCGACCTGCAGGGTCCGAAGATCCGCGTCGGCAAGTTCGAGAAC
GGCAAGATCACGCTTGAGCCGGGCGATCGCTTCACGCTCGACGCCCGCTGCGAGCTGGGCAACCAGGAGCGCGTCGGCCT
CGACTACAAGGAACTGCCGCGCGACGTTGGCCCGGGCGATCTGCTGCTGCTCAACGATGGGCTGATCGTGCTGCAAGTCG
AGCGCGTGGTGGGTGAAGAGATCGAGACCATCGTCAAGATCGGCGGCGATCTGTCCAACAACAAGGGCATCAACCGCCAG
GGCGGCGGCTTGTCGGCGCCGGCGCTGACCGCCAAGGACATGGAAGACATCAAGACCGCGATGGCGCTGGGCGCCGATTA
CGTCGCGGTCAGCTTCCCCAAGAACGCGACCGACATGGAAATGGCGCGCCAGTTGGCCGCCGTGGCCGGCGCACCGCACA
GCCACAAGACCCGCATGATCGCCAAGATCGAGCGCGCCGAGGCCATCCGCCCGGGCGTGCTCGAAGAGATCCTGGCTGCG
TCCGACGGCATCATGGTCGCGCGTGGCGACCTGGCGGTGGAAGTCGGCAACGCAGCCGTGCCGGCGCTCCAGAAGCGCAT
GATCAAGCTGGCCCGCGAAAACAATAAGCTGGCCATCACCGCCACGCAGATGATGGAATCGATGATCGTCAATCCGGTGC
CGACGCGCGCCGAGGTGTCGGACGTCGCCAACGCCGTGCTCGACGGTACCGATGCCGTGATGCTGTCGGCGGAAACCGCC
GCCGGCCGCTACCCGGTCGAGACCATCGAAGCCATGGCCGCCATCTGCGTGGAAGCCGAGAAGTCGCAAACCGTGCATCT
CGATGCAGATTTCCTCGACCAGACCTTCAGCCGTATCGACCAGTCGATCGCCATGGGGGCGCTGTTCACGGCGCACCACC
TGCAGGTCAAGGCAATTGCCGCGCTCACCGATTCCGGCGCTACGGCGCTCTGGATGAGCCGCCACGGCATCAACATCCCC
ATTTATGCGATGACGCCCAACGTGGCGTCGCAGCGCAAGATGGCCCTGTATCGCAACGTGCAGTCGCTGCCGCTGGCCAA
CAGCGCCGACCGCGACGAAGCCCTGCACCAAGCCGAAGAACTGCTGGTCGCGCGCGGCGTCGTGCAGCGTGGCGACTTCA
TCGTCCTGACCGTCGGCGAGCCAATGGGCCAGGCCGGCGGCACCAACACGCTCAAGATTGTTCGCGTCGGCATGCACTGA

Upstream 100 bases:

>100_bases
GCTGTCGAAATCCTGGAGCAACGCGCGCAGAATCAGGCCGCTGGGGCCTGACCGCCAGCGCCGCCAACATCCCCACCACA
ACAACGAGACACCGCCGCCC

Downstream 100 bases:

>100_bases
CGCCCACGACGGAACGCCGTTTGCTACGTCTACCAGACGTCCACGCGTTCCTGAGACCGAATACAGATCGATCCATAGGA
GAAAACCATGCCACTCGTCT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTRATKIVATLGPASSTQEVLTRMIAAGVDVVRLNFSHGAAQDHIDRAKLVREVARSVGREVAIMADLQGPKIRVGKFEN
GKITLEPGDRFTLDARCELGNQERVGLDYKELPRDVGPGDLLLLNDGLIVLQVERVVGEEIETIVKIGGDLSNNKGINRQ
GGGLSAPALTAKDMEDIKTAMALGADYVAVSFPKNATDMEMARQLAAVAGAPHSHKTRMIAKIERAEAIRPGVLEEILAA
SDGIMVARGDLAVEVGNAAVPALQKRMIKLARENNKLAITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETA
AGRYPVETIEAMAAICVEAEKSQTVHLDADFLDQTFSRIDQSIAMGALFTAHHLQVKAIAALTDSGATALWMSRHGINIP
IYAMTPNVASQRKMALYRNVQSLPLANSADRDEALHQAEELLVARGVVQRGDFIVLTVGEPMGQAGGTNTLKIVRVGMH

Sequences:

>Translated_479_residues
MTRATKIVATLGPASSTQEVLTRMIAAGVDVVRLNFSHGAAQDHIDRAKLVREVARSVGREVAIMADLQGPKIRVGKFEN
GKITLEPGDRFTLDARCELGNQERVGLDYKELPRDVGPGDLLLLNDGLIVLQVERVVGEEIETIVKIGGDLSNNKGINRQ
GGGLSAPALTAKDMEDIKTAMALGADYVAVSFPKNATDMEMARQLAAVAGAPHSHKTRMIAKIERAEAIRPGVLEEILAA
SDGIMVARGDLAVEVGNAAVPALQKRMIKLARENNKLAITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETA
AGRYPVETIEAMAAICVEAEKSQTVHLDADFLDQTFSRIDQSIAMGALFTAHHLQVKAIAALTDSGATALWMSRHGINIP
IYAMTPNVASQRKMALYRNVQSLPLANSADRDEALHQAEELLVARGVVQRGDFIVLTVGEPMGQAGGTNTLKIVRVGMH
>Mature_478_residues
TRATKIVATLGPASSTQEVLTRMIAAGVDVVRLNFSHGAAQDHIDRAKLVREVARSVGREVAIMADLQGPKIRVGKFENG
KITLEPGDRFTLDARCELGNQERVGLDYKELPRDVGPGDLLLLNDGLIVLQVERVVGEEIETIVKIGGDLSNNKGINRQG
GGLSAPALTAKDMEDIKTAMALGADYVAVSFPKNATDMEMARQLAAVAGAPHSHKTRMIAKIERAEAIRPGVLEEILAAS
DGIMVARGDLAVEVGNAAVPALQKRMIKLARENNKLAITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETAA
GRYPVETIEAMAAICVEAEKSQTVHLDADFLDQTFSRIDQSIAMGALFTAHHLQVKAIAALTDSGATALWMSRHGINIPI
YAMTPNVASQRKMALYRNVQSLPLANSADRDEALHQAEELLVARGVVQRGDFIVLTVGEPMGQAGGTNTLKIVRVGMH

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=498, Percent_Identity=37.3493975903614, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI33286420, Length=498, Percent_Identity=37.3493975903614, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI33286418, Length=501, Percent_Identity=37.5249500998004, Blast_Score=268, Evalue=9e-72,
Organism=Homo sapiens, GI10835121, Length=502, Percent_Identity=36.8525896414343, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI32967597, Length=502, Percent_Identity=36.8525896414343, Blast_Score=258, Evalue=9e-69,
Organism=Homo sapiens, GI310128732, Length=292, Percent_Identity=37.3287671232877, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI310128730, Length=292, Percent_Identity=37.3287671232877, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI310128736, Length=240, Percent_Identity=35, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI310128734, Length=240, Percent_Identity=35, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI310128738, Length=219, Percent_Identity=33.7899543378995, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=51.673640167364, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=37.8723404255319, Blast_Score=296, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=36.9609856262834, Blast_Score=275, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI71984406, Length=494, Percent_Identity=37.0445344129555, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17506831, Length=494, Percent_Identity=37.6518218623482, Blast_Score=248, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI71984413, Length=494, Percent_Identity=37.0445344129555, Blast_Score=248, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI17506829, Length=494, Percent_Identity=37.0445344129555, Blast_Score=248, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6319279, Length=495, Percent_Identity=33.9393939393939, Blast_Score=240, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324923, Length=495, Percent_Identity=35.1515151515151, Blast_Score=233, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=36.0323886639676, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=36.0323886639676, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24648966, Length=418, Percent_Identity=35.4066985645933, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24581235, Length=492, Percent_Identity=31.0975609756098, Blast_Score=203, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24646914, Length=184, Percent_Identity=42.9347826086957, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51197; Mature: 51066

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRATKIVATLGPASSTQEVLTRMIAAGVDVVRLNFSHGAAQDHIDRAKLVREVARSVGR
CCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EVAIMADLQGPKIRVGKFENGKITLEPGDRFTLDARCELGNQERVGLDYKELPRDVGPGD
EEEEEEECCCCEEEEEEECCCEEEECCCCEEEEEEEECCCCCCCCCCCHHHCCCCCCCCC
LLLLNDGLIVLQVERVVGEEIETIVKIGGDLSNNKGINRQGGGLSAPALTAKDMEDIKTA
EEEEECCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MALGADYVAVSFPKNATDMEMARQLAAVAGAPHSHKTRMIAKIERAEAIRPGVLEEILAA
HHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHC
SDGIMVARGDLAVEVGNAAVPALQKRMIKLARENNKLAITATQMMESMIVNPVPTRAEVS
CCCEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHH
DVANAVLDGTDAVMLSAETAAGRYPVETIEAMAAICVEAEKSQTVHLDADFLDQTFSRID
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHH
QSIAMGALFTAHHLQVKAIAALTDSGATALWMSRHGINIPIYAMTPNVASQRKMALYRNV
HHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHCH
QSLPLANSADRDEALHQAEELLVARGVVQRGDFIVLTVGEPMGQAGGTNTLKIVRVGMH
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TRATKIVATLGPASSTQEVLTRMIAAGVDVVRLNFSHGAAQDHIDRAKLVREVARSVGR
CCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
EVAIMADLQGPKIRVGKFENGKITLEPGDRFTLDARCELGNQERVGLDYKELPRDVGPGD
EEEEEEECCCCEEEEEEECCCEEEECCCCEEEEEEEECCCCCCCCCCCHHHCCCCCCCCC
LLLLNDGLIVLQVERVVGEEIETIVKIGGDLSNNKGINRQGGGLSAPALTAKDMEDIKTA
EEEEECCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MALGADYVAVSFPKNATDMEMARQLAAVAGAPHSHKTRMIAKIERAEAIRPGVLEEILAA
HHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHC
SDGIMVARGDLAVEVGNAAVPALQKRMIKLARENNKLAITATQMMESMIVNPVPTRAEVS
CCCEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHH
DVANAVLDGTDAVMLSAETAAGRYPVETIEAMAAICVEAEKSQTVHLDADFLDQTFSRID
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHH
QSIAMGALFTAHHLQVKAIAALTDSGATALWMSRHGINIPIYAMTPNVASQRKMALYRNV
HHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHCH
QSLPLANSADRDEALHQAEELLVARGVVQRGDFIVLTVGEPMGQAGGTNTLKIVRVGMH
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]