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The map label for this gene is dacB [H]

Identifier: 187935697

GI number: 187935697

Start: 2454333

End: 2455202

Strand: Reverse

Name: dacB [H]

Synonym: CLL_A2362

Alternate gene names: 187935697

Gene position: 2455202-2454333 (Counterclockwise)

Preceding gene: 187932772

Following gene: 187933205

Centisome position: 64.61

GC content: 28.85

Gene sequence:

>870_bases
ATGAGAAAAACTATTAAAAATTACATAGTAATAACAATGTTACTGAGTATTATGCTTTTTCAAATTTTCTCAAAAGCTGC
TTTAGCAGATAGCAAATTAAAAGATAATTTTAGAGTAAATGCAAGATGTGCTATAGCACTAGACAGAGAATCTAAAGAAG
TACTTTTTGAGCAAAATGCATATGAAATAGTACCTATGGCAAGTACAACAAAGATACTTACAGCAATAATTGCCATTGAG
CAAGGTGATTTAGATAAAAAAGTAGTTATAAGTAAGAAAGCTGCTTCAATAAGGGGATCAAAAGTGGGATATAAAGCAGG
AGAAGAAATAAGATTAAAAGAACTTGCTTTTGGTTTAATGTTTAAATCAGGAAATGATGCAGCAATAGCTATAGCTGAAA
CATTAGGAGGATCTGTTGAGGGATTTGCACAAATAATGAATCATTATGCAACAGGTATTGGAATAATAGATTCACATTTT
GAATCTCCTCATGGATTAGATAGTAATTCACATTATTCTTCAGCATATGATTTAGCTATTTTAACTGCCAAAGGTATGGA
ATATGATTTATTTAGAGAAATAGTTGGTTCAAAACAAATTAGTAAAGAAAAATATGATTTTACAAGAGACTATAACAATA
TAAACAAGATACTTTGGCTTATTCCAGAAGCAAATGGTGTTAAAACAGGTTATACAGGTGGAGCAGGAAAGTGCTTAGTT
TCATCTGTAAATCATAAAGGTAAAGATATAATTATAGTAGTTCTAAATTGTCCTGATAGATGGGCACAAACACAAAAAAT
ATATAATCATATATTAGAGACAGTTGCATTTGAAAATCATAATATTAAAGAACTAGTTTATAAAAATTAA

Upstream 100 bases:

>100_bases
TAAAATTAAAATTTCAAGGAAAAATAGAAGTAAGTAGTAATCAAGTAATTAATAATTGTAATTAATTATTAAGTAAGTAG
TAATTAAGAAGGTGAGTATT

Downstream 100 bases:

>100_bases
ATATTAAATAAGAAAATAGTCTGTTTAATTAAGAAAAACCTATCTTAAATTAAACAGACTATTTTATCTGTTAATTTTTA
ATATAATACTTGTTGAAGTT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRKTIKNYIVITMLLSIMLFQIFSKAALADSKLKDNFRVNARCAIALDRESKEVLFEQNAYEIVPMASTTKILTAIIAIE
QGDLDKKVVISKKAASIRGSKVGYKAGEEIRLKELAFGLMFKSGNDAAIAIAETLGGSVEGFAQIMNHYATGIGIIDSHF
ESPHGLDSNSHYSSAYDLAILTAKGMEYDLFREIVGSKQISKEKYDFTRDYNNINKILWLIPEANGVKTGYTGGAGKCLV
SSVNHKGKDIIIVVLNCPDRWAQTQKIYNHILETVAFENHNIKELVYKN

Sequences:

>Translated_289_residues
MRKTIKNYIVITMLLSIMLFQIFSKAALADSKLKDNFRVNARCAIALDRESKEVLFEQNAYEIVPMASTTKILTAIIAIE
QGDLDKKVVISKKAASIRGSKVGYKAGEEIRLKELAFGLMFKSGNDAAIAIAETLGGSVEGFAQIMNHYATGIGIIDSHF
ESPHGLDSNSHYSSAYDLAILTAKGMEYDLFREIVGSKQISKEKYDFTRDYNNINKILWLIPEANGVKTGYTGGAGKCLV
SSVNHKGKDIIIVVLNCPDRWAQTQKIYNHILETVAFENHNIKELVYKN
>Mature_289_residues
MRKTIKNYIVITMLLSIMLFQIFSKAALADSKLKDNFRVNARCAIALDRESKEVLFEQNAYEIVPMASTTKILTAIIAIE
QGDLDKKVVISKKAASIRGSKVGYKAGEEIRLKELAFGLMFKSGNDAAIAIAETLGGSVEGFAQIMNHYATGIGIIDSHF
ESPHGLDSNSHYSSAYDLAILTAKGMEYDLFREIVGSKQISKEKYDFTRDYNNINKILWLIPEANGVKTGYTGGAGKCLV
SSVNHKGKDIIIVVLNCPDRWAQTQKIYNHILETVAFENHNIKELVYKN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=257, Percent_Identity=35.7976653696498, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786851, Length=269, Percent_Identity=31.5985130111524, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082022, Length=237, Percent_Identity=29.535864978903, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87082054, Length=223, Percent_Identity=25.1121076233184, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 32108; Mature: 32108

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTIKNYIVITMLLSIMLFQIFSKAALADSKLKDNFRVNARCAIALDRESKEVLFEQNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECEEEEEEECCCCHHHHEECCC
YEIVPMASTTKILTAIIAIEQGDLDKKVVISKKAASIRGSKVGYKAGEEIRLKELAFGLM
EEEEECCHHHHHHHHHHEEECCCCCCEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHEEEE
FKSGNDAAIAIAETLGGSVEGFAQIMNHYATGIGIIDSHFESPHGLDSNSHYSSAYDLAI
EECCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCCCCCCEEEEEE
LTAKGMEYDLFREIVGSKQISKEKYDFTRDYNNINKILWLIPEANGVKTGYTGGAGKCLV
EEECCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHH
SSVNHKGKDIIIVVLNCPDRWAQTQKIYNHILETVAFENHNIKELVYKN
HHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure
MRKTIKNYIVITMLLSIMLFQIFSKAALADSKLKDNFRVNARCAIALDRESKEVLFEQNA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECEEEEEEECCCCHHHHEECCC
YEIVPMASTTKILTAIIAIEQGDLDKKVVISKKAASIRGSKVGYKAGEEIRLKELAFGLM
EEEEECCHHHHHHHHHHEEECCCCCCEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHEEEE
FKSGNDAAIAIAETLGGSVEGFAQIMNHYATGIGIIDSHFESPHGLDSNSHYSSAYDLAI
EECCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCCCCCCEEEEEE
LTAKGMEYDLFREIVGSKQISKEKYDFTRDYNNINKILWLIPEANGVKTGYTGGAGKCLV
EEECCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHH
SSVNHKGKDIIIVVLNCPDRWAQTQKIYNHILETVAFENHNIKELVYKN
HHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]