BacMap

Definition	Clostridium botulinum B str. Eklund 17B, complete genome.
Accession	NC_010674
Length	3,800,327

Click here to switch to the map view.

The map label for this gene is crt [H]

Identifier: 187935437

GI number: 187935437

Start: 2069303

End: 2070079

Strand: Reverse

Name: crt [H]

Synonym: CLL_A1990

Alternate gene names: 187935437

Gene position: 2070079-2069303 (Counterclockwise)

Preceding gene: 187934612

Following gene: 187933088

Centisome position: 54.47

GC content: 32.05

Gene sequence:

>777_bases
ATGGAAAATATTATTTTTAACGAAAAAAGTGGTATAGCAGAGGTGATAATAAATAGACCAAAGGCACTTAATGCATTGAA
TACAGCAACATTACAAGAATTGGGACAAGTGATAAATGAGATATCAGGAAGAAAAGATATTTATACTGTAATTATAACAG
GAGCTGGAGAAAAATCTTTTGTTGCTGGGGCAGATATAACTGAAATGAAAGAAAAAACTGCTATTGAAGGAAGAGAAATG
GCAATGCTTGCTCAAAAAGTATTTTCAAATATTGAAAATATGCCACAAGTTGTTATAGCAGCAGTAAACGGTTACGCTTT
AGGGGGAGGCTGTGAATTGTCAATGGCTTGTGATATAAGATTAGCGTCATCAAATGCCAAATTTGGACAACCAGAAGTTG
GATTAGGAATAATTCCAGGGTTTGCAGGAACTCAAAGGCTTCCTAGATTGGTTGGTAAAGGTATTGCAAAAGAGCTTATT
TTTACAACTGATATGATAGATGCTAATGAAGCTTATAGAATAGGGCTTGTTAATAAAATATATGAAAAAGATGAATTGAT
GGGTAAAGCAATGGAACTAGCAAATAAAATAATGCAAAAAGGAATGTATGCTGTAAGTTTAGCAAAATCATCTATAAATA
ATGGATTAAATATGGATACTGAATCAGCTTATGCTTTTGAAGCTAATTTATTTGGTTTATGTTTTTCAACAGAAGATCAA
ACAGAAGGCATGACAGCTTTTGTTGAAAAAAGAAAATCTTCATTTAAAGGATGTTAA

Upstream 100 bases:

>100_bases
ATGGAAAATAAATACAAACAAGTAATATGAAATTAATTAGAGAATATTGAGTAAAGATGTTTAGTAAATTATATAATTAA
AATTTAGGGAGGAAATCAAA

Downstream 100 bases:

>100_bases
AAAGGCAATTGTACTGACAGATTAATTAAAGACTACTAGGACATTAATTAATTTTAGGGGTTGATATAAATGAGTAAGGT
AAAGGTTTTGACTGGAGAGG

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MENIIFNEKSGIAEVIINRPKALNALNTATLQELGQVINEISGRKDIYTVIITGAGEKSFVAGADITEMKEKTAIEGREM
AMLAQKVFSNIENMPQVVIAAVNGYALGGGCELSMACDIRLASSNAKFGQPEVGLGIIPGFAGTQRLPRLVGKGIAKELI
FTTDMIDANEAYRIGLVNKIYEKDELMGKAMELANKIMQKGMYAVSLAKSSINNGLNMDTESAYAFEANLFGLCFSTEDQ
TEGMTAFVEKRKSSFKGC

Sequences:

>Translated_258_residues
MENIIFNEKSGIAEVIINRPKALNALNTATLQELGQVINEISGRKDIYTVIITGAGEKSFVAGADITEMKEKTAIEGREM
AMLAQKVFSNIENMPQVVIAAVNGYALGGGCELSMACDIRLASSNAKFGQPEVGLGIIPGFAGTQRLPRLVGKGIAKELI
FTTDMIDANEAYRIGLVNKIYEKDELMGKAMELANKIMQKGMYAVSLAKSSINNGLNMDTESAYAFEANLFGLCFSTEDQ
TEGMTAFVEKRKSSFKGC
>Mature_258_residues
MENIIFNEKSGIAEVIINRPKALNALNTATLQELGQVINEISGRKDIYTVIITGAGEKSFVAGADITEMKEKTAIEGREM
AMLAQKVFSNIENMPQVVIAAVNGYALGGGCELSMACDIRLASSNAKFGQPEVGLGIIPGFAGTQRLPRLVGKGIAKELI
FTTDMIDANEAYRIGLVNKIYEKDELMGKAMELANKIMQKGMYAVSLAKSSINNGLNMDTESAYAFEANLFGLCFSTEDQ
TEGMTAFVEKRKSSFKGC

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=42.96875, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=38.1889763779528, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=34.1269841269841, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI150378541, Length=251, Percent_Identity=30.6772908366534, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI20127408, Length=184, Percent_Identity=35.3260869565217, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI70995211, Length=195, Percent_Identity=33.8461538461538, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI68989263, Length=202, Percent_Identity=32.6732673267327, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI157694516, Length=232, Percent_Identity=27.5862068965517, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI213417737, Length=232, Percent_Identity=27.5862068965517, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI157694520, Length=209, Percent_Identity=29.1866028708134, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI37594469, Length=192, Percent_Identity=29.6875, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI37594471, Length=192, Percent_Identity=29.6875, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI261878539, Length=115, Percent_Identity=37.3913043478261, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=23.5294117647059, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI62530384, Length=203, Percent_Identity=24.6305418719212, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI4757968, Length=258, Percent_Identity=21.3178294573643, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI49355787, Length=258, Percent_Identity=21.3178294573643, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI25453481, Length=258, Percent_Identity=21.3178294573643, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI51538573, Length=258, Percent_Identity=21.3178294573643, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=37.1647509578544, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=35.3846153846154, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=37.9120879120879, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=37.5661375661376, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=29.811320754717, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI87082183, Length=252, Percent_Identity=30.5555555555556, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=27.5862068965517, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=40.5622489959839, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=39.5161290322581, Blast_Score=177, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=37.8378378378378, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17536985, Length=267, Percent_Identity=27.7153558052434, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=227, Percent_Identity=32.15859030837, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=26.7716535433071, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=228, Percent_Identity=32.8947368421053, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=228, Percent_Identity=32.8947368421053, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=228, Percent_Identity=32.8947368421053, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=251, Percent_Identity=29.0836653386454, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=240, Percent_Identity=29.1666666666667, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=191, Percent_Identity=31.9371727748691, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=191, Percent_Identity=31.9371727748691, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=187, Percent_Identity=31.0160427807487, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=216, Percent_Identity=26.3888888888889, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=238, Percent_Identity=26.0504201680672, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=43.4262948207171, Blast_Score=201, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=43.4262948207171, Blast_Score=201, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=37.9446640316205, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=29.4573643410853, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920382, Length=222, Percent_Identity=28.8288288288288, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654903, Length=173, Percent_Identity=28.9017341040462, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357171, Length=213, Percent_Identity=29.1079812206573, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571730, Length=153, Percent_Identity=32.6797385620915, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28571729, Length=153, Percent_Identity=32.6797385620915, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583077, Length=173, Percent_Identity=34.1040462427746, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24583079, Length=173, Percent_Identity=34.1040462427746, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921000, Length=173, Percent_Identity=34.1040462427746, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921018, Length=186, Percent_Identity=33.8709677419355, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24583165, Length=179, Percent_Identity=29.608938547486, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24650670, Length=211, Percent_Identity=27.4881516587678, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 27865; Mature: 27865

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIIFNEKSGIAEVIINRPKALNALNTATLQELGQVINEISGRKDIYTVIITGAGEKSF
CCCCEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCE
VAGADITEMKEKTAIEGREMAMLAQKVFSNIENMPQVVIAAVNGYALGGGCELSMACDIR
ECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHEEEEEECCEEECCCCEEEEEEEEE
LASSNAKFGQPEVGLGIIPGFAGTQRLPRLVGKGIAKELIFTTDMIDANEAYRIGLVNKI
EECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
YEKDELMGKAMELANKIMQKGMYAVSLAKSSINNGLNMDTESAYAFEANLFGLCFSTEDQ
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCCC
TEGMTAFVEKRKSSFKGC
CCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENIIFNEKSGIAEVIINRPKALNALNTATLQELGQVINEISGRKDIYTVIITGAGEKSF
CCCCEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCE
VAGADITEMKEKTAIEGREMAMLAQKVFSNIENMPQVVIAAVNGYALGGGCELSMACDIR
ECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHEEEEEECCEEECCCCEEEEEEEEE
LASSNAKFGQPEVGLGIIPGFAGTQRLPRLVGKGIAKELIFTTDMIDANEAYRIGLVNKI
EECCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
YEKDELMGKAMELANKIMQKGMYAVSLAKSSINNGLNMDTESAYAFEANLFGLCFSTEDQ
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCCCC
TEGMTAFVEKRKSSFKGC
CCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]