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The map label for this gene is amyS [H]

Identifier: 187935332

GI number: 187935332

Start: 2461612

End: 2463186

Strand: Reverse

Name: amyS [H]

Synonym: CLL_A2370

Alternate gene names: 187935332

Gene position: 2463186-2461612 (Counterclockwise)

Preceding gene: 187934387

Following gene: 187932329

Centisome position: 64.82

GC content: 28.51

Gene sequence:

>1575_bases
ATGACAGTAGAAATTATTTTAATAATAATCGTATTTGCAATAATAGGAGGAGTAATGTATTTAAATTTAGAACAAGACAA
AGCTAAGGATGTAGAAAAAAATGAAGATAAAGTTTACATAGAAGATAAAAAAATCAAAAACAAAACTATGATGCAATATT
TTGAATGGTATTACCCTAATGATGGTTCTCTTTGGAATAAGGTAAAAGAACATTCTAAGAAATTAAGTAAAAAAGGGATT
ACTGCATTGTGGCTACCACCTGCTTATAAAGGGGTAGGTGGTATTAATGATGTTGGGTATGGAGCGTATGACTTATATGA
TCTAGGAGAATTTGATCAAAAAGGAACCATAAGAACTAAATATGGTACTAAAGAACAATATTTGGATGCGATAAATGAAG
CACATAATAATAATATAGAAGTATATGCAGATACTGTATTTAATCATAAAGCAGGTGCAGATGAAAGTGAATGTGTAAAG
GCGCAACTAGTAGATAGCAATAACAGAAATAATATTATAGGAGAAGAAAAAGAAATAAGGTCATTTACTGTATTTAATTT
TTCAGGAAGGAATGACAAGTATTCTTCTTATAAATGGAATGCAAAAGATTTTGATGGAGTAGATTTTGATGATTTAACAA
AACAAAATGGAATATTTAAATTTGTAGGGAAAGAATGGGAACAGGATGTAGATGATGAAAATGGAAATTTTGATTTTTTA
ATGTGTGCAGATTTAGATATAGATAGTAGAGATGTTGTAGAAGAGCTTAAAACATGGGGGAAATGGTATATAAATGAATG
TAAGCTAGATGGTTTCAGATTAGATGCAATAAAACATATAAAATTTGATTTTTTTGCAGAATGGTTAAATGATATTAGAA
GTAAAAAAGGTGACAATGTATTTGCAGTGGGTGAATATTGGTCTGGAGATATAAATAAATTAAAATATTACATTAGTAAA
TCACAAAATGTTATGTCGTTATTTGATGTGCCACTTCATTATAAATTTTTTGAAGCAAGCAATTTAGAAGAAAACTTTGA
TATGAGAACATTAACTCAAAATACTTTATTAGATTATGATGAAAAAATCTCAGTGACTTTTGTTGATAATCATGACACTG
AACCAGGTCAGGCTCTTGAATCTTGGGTTAAGCCATGGTTTAAATTAATAGCATACACATTTATATTAACAAGGCAAGAA
GGATATCCATGTATATTTTACGGAGATTATTATGGAGTTCCTGAAAAGGGATTTGAGGGGTTTAAGAATCAGTTGGACAT
GATTTTAAATGTAAGAAAAGACTATGCTTACGGTGATCAATGTGATTATTTTGATGATAAAAATATAATTGGGTGGACTA
GACAAGGCGATTTAATGCACGATAATTCAGGTGTTGCGGTATTAATAAGTAATGGTAATAATGGCAGTAAAAAGATGTCT
ATGGGGAGCAAAAATATAAGTACTACTTTTATAGATATAACAGGAAATATACAAGAAGAAATACTTATAGATAATGAAGG
TAATGGAATTTTTAAAGTAAATAATAAATCATATTCAATATGGATTAAAAAATAA

Upstream 100 bases:

>100_bases
TATATGATTTAAGATAGGAATTAATCCTATCTTAATATTTAGAAACAAATTGGTATCGTTACCGAAAGCATAATTTCAGT
GAATTAAGGAGAGGATAATA

Downstream 100 bases:

>100_bases
TAAAAAAATTTTTTAGATGACTTAATACGAGTCATCTATTTTTTATGTAATTAAAAAGTTTATAAACCAGGTGTTTATCA
CATTTTTAAAGAGTTTATAA

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTVEIILIIIVFAIIGGVMYLNLEQDKAKDVEKNEDKVYIEDKKIKNKTMMQYFEWYYPNDGSLWNKVKEHSKKLSKKGI
TALWLPPAYKGVGGINDVGYGAYDLYDLGEFDQKGTIRTKYGTKEQYLDAINEAHNNNIEVYADTVFNHKAGADESECVK
AQLVDSNNRNNIIGEEKEIRSFTVFNFSGRNDKYSSYKWNAKDFDGVDFDDLTKQNGIFKFVGKEWEQDVDDENGNFDFL
MCADLDIDSRDVVEELKTWGKWYINECKLDGFRLDAIKHIKFDFFAEWLNDIRSKKGDNVFAVGEYWSGDINKLKYYISK
SQNVMSLFDVPLHYKFFEASNLEENFDMRTLTQNTLLDYDEKISVTFVDNHDTEPGQALESWVKPWFKLIAYTFILTRQE
GYPCIFYGDYYGVPEKGFEGFKNQLDMILNVRKDYAYGDQCDYFDDKNIIGWTRQGDLMHDNSGVAVLISNGNNGSKKMS
MGSKNISTTFIDITGNIQEEILIDNEGNGIFKVNNKSYSIWIKK

Sequences:

>Translated_524_residues
MTVEIILIIIVFAIIGGVMYLNLEQDKAKDVEKNEDKVYIEDKKIKNKTMMQYFEWYYPNDGSLWNKVKEHSKKLSKKGI
TALWLPPAYKGVGGINDVGYGAYDLYDLGEFDQKGTIRTKYGTKEQYLDAINEAHNNNIEVYADTVFNHKAGADESECVK
AQLVDSNNRNNIIGEEKEIRSFTVFNFSGRNDKYSSYKWNAKDFDGVDFDDLTKQNGIFKFVGKEWEQDVDDENGNFDFL
MCADLDIDSRDVVEELKTWGKWYINECKLDGFRLDAIKHIKFDFFAEWLNDIRSKKGDNVFAVGEYWSGDINKLKYYISK
SQNVMSLFDVPLHYKFFEASNLEENFDMRTLTQNTLLDYDEKISVTFVDNHDTEPGQALESWVKPWFKLIAYTFILTRQE
GYPCIFYGDYYGVPEKGFEGFKNQLDMILNVRKDYAYGDQCDYFDDKNIIGWTRQGDLMHDNSGVAVLISNGNNGSKKMS
MGSKNISTTFIDITGNIQEEILIDNEGNGIFKVNNKSYSIWIKK
>Mature_523_residues
TVEIILIIIVFAIIGGVMYLNLEQDKAKDVEKNEDKVYIEDKKIKNKTMMQYFEWYYPNDGSLWNKVKEHSKKLSKKGIT
ALWLPPAYKGVGGINDVGYGAYDLYDLGEFDQKGTIRTKYGTKEQYLDAINEAHNNNIEVYADTVFNHKAGADESECVKA
QLVDSNNRNNIIGEEKEIRSFTVFNFSGRNDKYSSYKWNAKDFDGVDFDDLTKQNGIFKFVGKEWEQDVDDENGNFDFLM
CADLDIDSRDVVEELKTWGKWYINECKLDGFRLDAIKHIKFDFFAEWLNDIRSKKGDNVFAVGEYWSGDINKLKYYISKS
QNVMSLFDVPLHYKFFEASNLEENFDMRTLTQNTLLDYDEKISVTFVDNHDTEPGQALESWVKPWFKLIAYTFILTRQEG
YPCIFYGDYYGVPEKGFEGFKNQLDMILNVRKDYAYGDQCDYFDDKNIIGWTRQGDLMHDNSGVAVLISNGNNGSKKMSM
GSKNISTTFIDITGNIQEEILIDNEGNGIFKVNNKSYSIWIKK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=492, Percent_Identity=41.260162601626, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 60667; Mature: 60535

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVEIILIIIVFAIIGGVMYLNLEQDKAKDVEKNEDKVYIEDKKIKNKTMMQYFEWYYPN
CCHHHHHHHHHHHHHCCEEEEEECHHHCCCCCCCCCEEEEECCHHCHHHHHHHHHHCCCC
DGSLWNKVKEHSKKLSKKGITALWLPPAYKGVGGINDVGYGAYDLYDLGEFDQKGTIRTK
CCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEC
YGTKEQYLDAINEAHNNNIEVYADTVFNHKAGADESECVKAQLVDSNNRNNIIGEEKEIR
CCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHEEEEEECCCCCCCCCCCCHHCC
SFTVFNFSGRNDKYSSYKWNAKDFDGVDFDDLTKQNGIFKFVGKEWEQDVDDENGNFDFL
EEEEEEECCCCCCCCCEECCCCCCCCCCHHHHHCCCCEEEECCCHHHHCCCCCCCCEEEE
MCADLDIDSRDVVEELKTWGKWYINECKLDGFRLDAIKHIKFDFFAEWLNDIRSKKGDNV
EEECCCCCHHHHHHHHHHHHHHEEEEEEECCEEECHHHHHHHHHHHHHHHHHHHCCCCCE
FAVGEYWSGDINKLKYYISKSQNVMSLFDVPLHYKFFEASNLEENFDMRTLTQNTLLDYD
EEECCCCCCCHHHHHEEHHCCCCEEEEECCCCEEEEEECCCCCCCCCHHHCCHHHHCCCC
EKISVTFVDNHDTEPGQALESWVKPWFKLIAYTFILTRQEGYPCIFYGDYYGVPEKGFEG
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCCHHHHH
FKNQLDMILNVRKDYAYGDQCDYFDDKNIIGWTRQGDLMHDNSGVAVLISNGNNGSKKMS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEECCCCEEEEEECCCCCCCEEE
MGSKNISTTFIDITGNIQEEILIDNEGNGIFKVNNKSYSIWIKK
CCCCCCEEEEEEEECCCEEEEEEECCCCEEEEECCCEEEEEEEC
>Mature Secondary Structure 
TVEIILIIIVFAIIGGVMYLNLEQDKAKDVEKNEDKVYIEDKKIKNKTMMQYFEWYYPN
CHHHHHHHHHHHHHCCEEEEEECHHHCCCCCCCCCEEEEECCHHCHHHHHHHHHHCCCC
DGSLWNKVKEHSKKLSKKGITALWLPPAYKGVGGINDVGYGAYDLYDLGEFDQKGTIRTK
CCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEC
YGTKEQYLDAINEAHNNNIEVYADTVFNHKAGADESECVKAQLVDSNNRNNIIGEEKEIR
CCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHEEEEEECCCCCCCCCCCCHHCC
SFTVFNFSGRNDKYSSYKWNAKDFDGVDFDDLTKQNGIFKFVGKEWEQDVDDENGNFDFL
EEEEEEECCCCCCCCCEECCCCCCCCCCHHHHHCCCCEEEECCCHHHHCCCCCCCCEEEE
MCADLDIDSRDVVEELKTWGKWYINECKLDGFRLDAIKHIKFDFFAEWLNDIRSKKGDNV
EEECCCCCHHHHHHHHHHHHHHEEEEEEECCEEECHHHHHHHHHHHHHHHHHHHCCCCCE
FAVGEYWSGDINKLKYYISKSQNVMSLFDVPLHYKFFEASNLEENFDMRTLTQNTLLDYD
EEECCCCCCCHHHHHEEHHCCCCEEEEECCCCEEEEEECCCCCCCCCHHHCCHHHHCCCC
EKISVTFVDNHDTEPGQALESWVKPWFKLIAYTFILTRQEGYPCIFYGDYYGVPEKGFEG
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCCHHHHH
FKNQLDMILNVRKDYAYGDQCDYFDDKNIIGWTRQGDLMHDNSGVAVLISNGNNGSKKMS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCEEECCCCEEEEEECCCCCCCEEE
MGSKNISTTFIDITGNIQEEILIDNEGNGIFKVNNKSYSIWIKK
CCCCCCEEEEEEEECCCEEEEEEECCCCEEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924897; 3876333; 3009417; 2999073; 11226887 [H]