| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is aroF [H]
Identifier: 187935227
GI number: 187935227
Start: 3254177
End: 3255190
Strand: Reverse
Name: aroF [H]
Synonym: CLL_A3144
Alternate gene names: 187935227
Gene position: 3255190-3254177 (Counterclockwise)
Preceding gene: 187934416
Following gene: 187933372
Centisome position: 85.66
GC content: 31.66
Gene sequence:
>1014_bases ATGATAATAATATTAAAGCAAAAAGCAGAACAAGAAGAAATTGAAAAATTAACTTCAATTTTAGAAAATCAAGGTGTTAA GGTAAATCCTGTTATAGGAACAGACATTAGCATTTTAGGTTTAGTAGGAGATACTAGTAGAATTGATAAGGAGCAAATTG AAGCTAATGAAATAGTTGAAAAAGTTATGCATGTACAAGAACCTTTTAAAAAGGCAAATAGAATGTTTCATCCAGAAGCA ACAATAATTGATGTGAATGGACAAAAAATAGGTGGAAATAAAATAGCTATGATTGCAGGTCCATGCTCAGTTGAAAGTGA AGAGCAAATAATTAAAATAGCTGAAGGGGTTAAAAATATAGGGGCAAATTTTTTAAGAGGGGGAGCATTTAAACCTAGAA CGTCACCATACGCATTCCAAGGACTAAAGTATGAAGGATTAGAACTTTTAAAAAAAGCAAAAGCTAAGACGGGTTTACCT ATAGTTACTGAAATTATGTCACCATACGATATAGAATTCTTTAATGACAATGTAGATGTTATTCAAGTTGGGGCAAGAAA TATGCAAAATTTTGATTTACTTAAAGAATTGGGACAATTGAATAAACCAATACTTTTAAAGAGAGGATTATCAGCTACTC TTGAGGAATTATTAATGAGTGCTGAATATATAATGGCTGGTGGAAATGAAAATGTAATTCTTTGTGAAAGAGGAATTAGA ACTTTTGAGACATACACTAGAAATACTTTAGATTTAAGTGCAGTTCCAGCTCTTAAAAAGTTAAGTCATTTACCAGTTGT TATTGATCCAAGTCATGCTACAGGAAAGTATTGGATGGTAGAACCACTAGCGAAAGCTGCAATTGCAATTGGTGCAGATG GTCTTATAATAGAAGTTCATAATGATCCAGCAAATGCATTATGTGATGGTCCACAATCAATAAAGCCGGAAAAATATGCA AGAATTTTTGAAGAATTAAAAGTTATTGCAAAAGCAGTAGGTAGAGAAATATAG
Upstream 100 bases:
>100_bases TATTAAAAAGTATCAAATTAAAGAAATATTTAGTTTTTATCTGTAAGTTAACAAAGTAAGAATTAACAACTTTGTTATAT AAGATTAAGGGGGCAAATAT
Downstream 100 bases:
>100_bases TTTAAATAAAGTTAAATTTATACTGAAAGTGAGATTGTAATTATAATATAAAGCTTATGGACTAAAGAATTTGCTTTAAG AGTTCTAAAAATCAAATTCT
Product: 3-deoxy-7-phosphoheptulonate synthase
Products: NA
Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]
Number of amino acids: Translated: 337; Mature: 337
Protein sequence:
>337_residues MIIILKQKAEQEEIEKLTSILENQGVKVNPVIGTDISILGLVGDTSRIDKEQIEANEIVEKVMHVQEPFKKANRMFHPEA TIIDVNGQKIGGNKIAMIAGPCSVESEEQIIKIAEGVKNIGANFLRGGAFKPRTSPYAFQGLKYEGLELLKKAKAKTGLP IVTEIMSPYDIEFFNDNVDVIQVGARNMQNFDLLKELGQLNKPILLKRGLSATLEELLMSAEYIMAGGNENVILCERGIR TFETYTRNTLDLSAVPALKKLSHLPVVIDPSHATGKYWMVEPLAKAAIAIGADGLIIEVHNDPANALCDGPQSIKPEKYA RIFEELKVIAKAVGREI
Sequences:
>Translated_337_residues MIIILKQKAEQEEIEKLTSILENQGVKVNPVIGTDISILGLVGDTSRIDKEQIEANEIVEKVMHVQEPFKKANRMFHPEA TIIDVNGQKIGGNKIAMIAGPCSVESEEQIIKIAEGVKNIGANFLRGGAFKPRTSPYAFQGLKYEGLELLKKAKAKTGLP IVTEIMSPYDIEFFNDNVDVIQVGARNMQNFDLLKELGQLNKPILLKRGLSATLEELLMSAEYIMAGGNENVILCERGIR TFETYTRNTLDLSAVPALKKLSHLPVVIDPSHATGKYWMVEPLAKAAIAIGADGLIIEVHNDPANALCDGPQSIKPEKYA RIFEELKVIAKAVGREI >Mature_337_residues MIIILKQKAEQEEIEKLTSILENQGVKVNPVIGTDISILGLVGDTSRIDKEQIEANEIVEKVMHVQEPFKKANRMFHPEA TIIDVNGQKIGGNKIAMIAGPCSVESEEQIIKIAEGVKNIGANFLRGGAFKPRTSPYAFQGLKYEGLELLKKAKAKTGLP IVTEIMSPYDIEFFNDNVDVIQVGARNMQNFDLLKELGQLNKPILLKRGLSATLEELLMSAEYIMAGGNENVILCERGIR TFETYTRNTLDLSAVPALKKLSHLPVVIDPSHATGKYWMVEPLAKAAIAIGADGLIIEVHNDPANALCDGPQSIKPEKYA RIFEELKVIAKAVGREI
Specific function: Catalyzes the condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]
COG id: COG2876
COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the class-I DAHP synthase family [H]
Homologues:
Organism=Escherichia coli, GI1787466, Length=252, Percent_Identity=27.7777777777778, Blast_Score=94, Evalue=1e-20,
Paralogues:
None
Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013785 - InterPro: IPR006218 - InterPro: IPR006268 [H]
Pfam domain/function: PF00793 DAHP_synth_1 [H]
EC number: =2.5.1.54 [H]
Molecular weight: Translated: 37070; Mature: 37070
Theoretical pI: Translated: 5.79; Mature: 5.79
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIIILKQKAEQEEIEKLTSILENQGVKVNPVIGTDISILGLVGDTSRIDKEQIEANEIVE CEEEEECCCCHHHHHHHHHHHHCCCCEEECEECCCEEEEEEECCCCCCCHHHHHHHHHHH KVMHVQEPFKKANRMFHPEATIIDVNGQKIGGNKIAMIAGPCSVESEEQIIKIAEGVKNI HHHHHHHHHHHHHCCCCCCCEEEECCCCEECCCEEEEEECCCCCCCHHHHHHHHHHHHHH GANFLRGGAFKPRTSPYAFQGLKYEGLELLKKAKAKTGLPIVTEIMSPYDIEFFNDNVDV CHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCE IQVGARNMQNFDLLKELGQLNKPILLKRGLSATLEELLMSAEYIMAGGNENVILCERGIR EEECCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHEEECCCCCEEEECCCCH TFETYTRNTLDLSAVPALKKLSHLPVVIDPSHATGKYWMVEPLAKAAIAIGADGLIIEVH HHHHHCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEHHHHHHHHHHCCCCEEEEEEE NDPANALCDGPQSIKPEKYARIFEELKVIAKAVGREI CCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC >Mature Secondary Structure MIIILKQKAEQEEIEKLTSILENQGVKVNPVIGTDISILGLVGDTSRIDKEQIEANEIVE CEEEEECCCCHHHHHHHHHHHHCCCCEEECEECCCEEEEEEECCCCCCCHHHHHHHHHHH KVMHVQEPFKKANRMFHPEATIIDVNGQKIGGNKIAMIAGPCSVESEEQIIKIAEGVKNI HHHHHHHHHHHHHCCCCCCCEEEECCCCEECCCEEEEEECCCCCCCHHHHHHHHHHHHHH GANFLRGGAFKPRTSPYAFQGLKYEGLELLKKAKAKTGLPIVTEIMSPYDIEFFNDNVDV CHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCE IQVGARNMQNFDLLKELGQLNKPILLKRGLSATLEELLMSAEYIMAGGNENVILCERGIR EEECCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHEEECCCCCEEEECCCCH TFETYTRNTLDLSAVPALKKLSHLPVVIDPSHATGKYWMVEPLAKAAIAIGADGLIIEVH HHHHHCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEHHHHHHHHHHCCCCEEEEEEE NDPANALCDGPQSIKPEKYARIFEELKVIAKAVGREI CCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 10360571; 12743122 [H]