Click here to switch to the map view.

The map label for this gene is pbpA [H]

Identifier: 187935186

GI number: 187935186

Start: 1011604

End: 1014117

Strand: Reverse

Name: pbpA [H]

Synonym: CLL_A1001

Alternate gene names: 187935186

Gene position: 1014117-1011604 (Counterclockwise)

Preceding gene: 187934669

Following gene: 187935102

Centisome position: 26.68

GC content: 31.3

Gene sequence:

>2514_bases
ATGTCGACTGAAAAAACTCCAAATAAAACAAGAAAAAGTACTAATAATAAAAGAAAAAAGAAGAAAAAGAAAAAAAATAA
ATTAACTGCCTCTACTATCTTAAAGAAAATAGGTATTGGGGTTTTGACTTTGATATTAATAGTATCTGTTGTAGGTGCAG
GATACTTACTTGCAATTATAAAAACTACCCCGCCATTAGATGTTGAAGATGTTTTATCGTTAAATCAAGCTTCAAGTTTA
TATGATAGTAATGAAGAATTTATGGATAATTTACATACAGATGAAGAACGATATGTGATAACTTCTGATAAAATTCCAGA
TAACCTTAAAAATGCCTTTATATCAATTGAAGATGAACGTTTTTTTAAACATAGAGGAATAGATGTTCAAAGAATATTAG
GAGCTGCTTTAACAGATGTAAAGAAAATTTTAACTGGACAAAAAGGGTTACATGGCGCTTCTACAATAACTCAACAACTT
ATTAAAAACACAGTTCTTACTAATGAAGTATCTATCAAAAGAAAAGTAAAGGAAATTTACTTATCTCTTGATCTTGAAGA
CCAGTTAAGTAAAGATCAAATAATTACTGCATATTTAAATACAATACCTCTTGGTGGTCAAGTGTATGGCGTTGAAGCAG
CCTCATTGCTATATTTTAGTAAAAACACATCTGATTTAAGTTTAATTGAATGTGCTTATTTAGCAGGTATAACTCAAGCA
CCAAGCTATTATAGTGCTTATAATGAAAATAGCCAAAAAGATCCTTCAAGGTATATTAACAGAACCTTAACTGTTTTATC
TAAAATGAATGAACTAGGTTATATTTCAGAAAGTGAATATAATAAAGGTGTTCAAGATATTAATGACGGCAAACTAGCTT
TTAAATCAAGTAAAAAAGATTTTCGCTTAAATTATGAGTGGTTTGTTTATCCAGCTGTATCTCAAGTTAAGAGAGATTTA
AAAGAAAAATACAAATACACAGATGAGGAAGTTTCTAAATTGATTGTTAATGGTGGTTTAAAAATTTATACTACTATGGA
TAGGGATTTACAAGATTTCACTCAAAAAACTTTAGATAACTATAAAAATTTAAATGTTGGAAATTCGGAAGCTTATGATG
AAAATAAAGTTCCATTATTGCAAGCTTCAGCAACAATAACAGACTATAGAAATGGTAAAGTTCTTGCTATGGTTGGTGGA
AGAGGTAAACAACTCCCTCAGTCAAATAATAGAGCTTATAATGATTTAAGATCAATAGGTTCTACTACTAAGCCACTTAC
TGTATATGGACCTGGTATAGATCAAGAGATAATAACGGCTGCAACATCAATAGATGATGCTCCTTTATCACCAGAATTAA
CAAAAAAATATCCAGCATATGCTCCACCTAATATTCTTAGGAATTCACCAGATGAATACTTAGGTCTTATATCTCCACGT
GAAGGAATAATGTATTCTAAAAATACTGCTTCTGTTATAACTGCAGACACTATTGGTTTAAAAACAGGAATATCTTATGG
AGAAAATTTAGGTCTTGTATATAACAGTGCATCTAAAACTTCTATAGCTACTGTTGCATTAGGAGAATTTAATAATGATC
CTAAAGATAGAGATGGTGGTAACACTTATAAAGTGGCTGCTGCATTTGGAAGCTTTGGTAATGATGGTCTATACGTAGAA
CCTCTACTTTATACAAAAGTAGTTGATCCAAAAGGTAATACAGTATTAGAAGGAAACCCAAAAGAAAAACAAGTATTTTC
ACCAGAGACTGCATATATAATGTATGACTTATTGAAAGGACCCGTTAACTTTTATGGTAGTGCTCCTGCTAAGTGGAGTG
ATATGCCTGTTGCTGGTAAAACAGGTACTACTACAGATGCAAAAGACTTATGGTTTGCAGGACTTACGCCTTATCTATCA
GGATCAGTTTGGGTTGGTTATGATAATCCTAAAACTGTATTTGGTGGATCTGGAACTGTATGTGCTAATTTATGGGGAAA
AATAATGGCTAAAGCACATAAAGGAATGGAAGTTAAAGATTTAGAGGAACCTAGCGGAATTGTTCACGTTGCTGTATGTA
AAGATTCAGGTAAGTTGCCATCAAATTTATGTAGTAGTGATCCTAGAGGAAATAGAATTACAGAAGAATTATTTATTGAA
GGAACTGAACCACATGAAACATGTAATACTCATATTAGCGGGCGTATAAATAGACTTAATAATAAATTAGCTGGCGCTAA
TACGCCTGCATTATTAACTCAAAATAGAGTTTTTATAAGCAAAAAATATCCTAATCCATTAACTAGAGATTATATATATG
TACTTCCACATTCACAAGATAATTATATAGAAGATCAAGCTAATCCAGAGCTTTCTCCTCCAAACGTAAATGAGGAAGGT
GAAGTAGAAATTCCAAACAATAATGGTACTCCTCAGGGAAATAATAAACCACCTGAGACTGAGCAAAATGACTCAATATT
AAATTCTATACTAAAACCATTAAAACCACATTAA

Upstream 100 bases:

>100_bases
TCACTACCTTTATGACGAGTTATTAGTTTACTCTTATTATAAGTATGCTATAATAATAAAGAATATTATTTAATCATTAA
CGTAATTTTAGGAGGATAGA

Downstream 100 bases:

>100_bases
TATGAAAAAACGACACATTAATTTTTTAATGTGTTGTTTTTTTATTAAAATAAAAAAAATTCCGTATTCTATTTATTATA
TAGCATAAAATATATATATA

Product: membrane carboxypeptidase MrcB

Products: NA

Alternate protein names: PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 837; Mature: 836

Protein sequence:

>837_residues
MSTEKTPNKTRKSTNNKRKKKKKKKNKLTASTILKKIGIGVLTLILIVSVVGAGYLLAIIKTTPPLDVEDVLSLNQASSL
YDSNEEFMDNLHTDEERYVITSDKIPDNLKNAFISIEDERFFKHRGIDVQRILGAALTDVKKILTGQKGLHGASTITQQL
IKNTVLTNEVSIKRKVKEIYLSLDLEDQLSKDQIITAYLNTIPLGGQVYGVEAASLLYFSKNTSDLSLIECAYLAGITQA
PSYYSAYNENSQKDPSRYINRTLTVLSKMNELGYISESEYNKGVQDINDGKLAFKSSKKDFRLNYEWFVYPAVSQVKRDL
KEKYKYTDEEVSKLIVNGGLKIYTTMDRDLQDFTQKTLDNYKNLNVGNSEAYDENKVPLLQASATITDYRNGKVLAMVGG
RGKQLPQSNNRAYNDLRSIGSTTKPLTVYGPGIDQEIITAATSIDDAPLSPELTKKYPAYAPPNILRNSPDEYLGLISPR
EGIMYSKNTASVITADTIGLKTGISYGENLGLVYNSASKTSIATVALGEFNNDPKDRDGGNTYKVAAAFGSFGNDGLYVE
PLLYTKVVDPKGNTVLEGNPKEKQVFSPETAYIMYDLLKGPVNFYGSAPAKWSDMPVAGKTGTTTDAKDLWFAGLTPYLS
GSVWVGYDNPKTVFGGSGTVCANLWGKIMAKAHKGMEVKDLEEPSGIVHVAVCKDSGKLPSNLCSSDPRGNRITEELFIE
GTEPHETCNTHISGRINRLNNKLAGANTPALLTQNRVFISKKYPNPLTRDYIYVLPHSQDNYIEDQANPELSPPNVNEEG
EVEIPNNNGTPQGNNKPPETEQNDSILNSILKPLKPH

Sequences:

>Translated_837_residues
MSTEKTPNKTRKSTNNKRKKKKKKKNKLTASTILKKIGIGVLTLILIVSVVGAGYLLAIIKTTPPLDVEDVLSLNQASSL
YDSNEEFMDNLHTDEERYVITSDKIPDNLKNAFISIEDERFFKHRGIDVQRILGAALTDVKKILTGQKGLHGASTITQQL
IKNTVLTNEVSIKRKVKEIYLSLDLEDQLSKDQIITAYLNTIPLGGQVYGVEAASLLYFSKNTSDLSLIECAYLAGITQA
PSYYSAYNENSQKDPSRYINRTLTVLSKMNELGYISESEYNKGVQDINDGKLAFKSSKKDFRLNYEWFVYPAVSQVKRDL
KEKYKYTDEEVSKLIVNGGLKIYTTMDRDLQDFTQKTLDNYKNLNVGNSEAYDENKVPLLQASATITDYRNGKVLAMVGG
RGKQLPQSNNRAYNDLRSIGSTTKPLTVYGPGIDQEIITAATSIDDAPLSPELTKKYPAYAPPNILRNSPDEYLGLISPR
EGIMYSKNTASVITADTIGLKTGISYGENLGLVYNSASKTSIATVALGEFNNDPKDRDGGNTYKVAAAFGSFGNDGLYVE
PLLYTKVVDPKGNTVLEGNPKEKQVFSPETAYIMYDLLKGPVNFYGSAPAKWSDMPVAGKTGTTTDAKDLWFAGLTPYLS
GSVWVGYDNPKTVFGGSGTVCANLWGKIMAKAHKGMEVKDLEEPSGIVHVAVCKDSGKLPSNLCSSDPRGNRITEELFIE
GTEPHETCNTHISGRINRLNNKLAGANTPALLTQNRVFISKKYPNPLTRDYIYVLPHSQDNYIEDQANPELSPPNVNEEG
EVEIPNNNGTPQGNNKPPETEQNDSILNSILKPLKPH
>Mature_836_residues
STEKTPNKTRKSTNNKRKKKKKKKNKLTASTILKKIGIGVLTLILIVSVVGAGYLLAIIKTTPPLDVEDVLSLNQASSLY
DSNEEFMDNLHTDEERYVITSDKIPDNLKNAFISIEDERFFKHRGIDVQRILGAALTDVKKILTGQKGLHGASTITQQLI
KNTVLTNEVSIKRKVKEIYLSLDLEDQLSKDQIITAYLNTIPLGGQVYGVEAASLLYFSKNTSDLSLIECAYLAGITQAP
SYYSAYNENSQKDPSRYINRTLTVLSKMNELGYISESEYNKGVQDINDGKLAFKSSKKDFRLNYEWFVYPAVSQVKRDLK
EKYKYTDEEVSKLIVNGGLKIYTTMDRDLQDFTQKTLDNYKNLNVGNSEAYDENKVPLLQASATITDYRNGKVLAMVGGR
GKQLPQSNNRAYNDLRSIGSTTKPLTVYGPGIDQEIITAATSIDDAPLSPELTKKYPAYAPPNILRNSPDEYLGLISPRE
GIMYSKNTASVITADTIGLKTGISYGENLGLVYNSASKTSIATVALGEFNNDPKDRDGGNTYKVAAAFGSFGNDGLYVEP
LLYTKVVDPKGNTVLEGNPKEKQVFSPETAYIMYDLLKGPVNFYGSAPAKWSDMPVAGKTGTTTDAKDLWFAGLTPYLSG
SVWVGYDNPKTVFGGSGTVCANLWGKIMAKAHKGMEVKDLEEPSGIVHVAVCKDSGKLPSNLCSSDPRGNRITEELFIEG
TEPHETCNTHISGRINRLNNKLAGANTPALLTQNRVFISKKYPNPLTRDYIYVLPHSQDNYIEDQANPELSPPNVNEEGE
VEIPNNNGTPQGNNKPPETEQNDSILNSILKPLKPH

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=584, Percent_Identity=27.7397260273973, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI87082258, Length=376, Percent_Identity=31.1170212765957, Blast_Score=149, Evalue=9e-37,
Organism=Escherichia coli, GI1788867, Length=645, Percent_Identity=23.8759689922481, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1789601, Length=250, Percent_Identity=30.4, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 92366; Mature: 92235

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEKTPNKTRKSTNNKRKKKKKKKNKLTASTILKKIGIGVLTLILIVSVVGAGYLLAII
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
KTTPPLDVEDVLSLNQASSLYDSNEEFMDNLHTDEERYVITSDKIPDNLKNAFISIEDER
ECCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCHHHHEEEECHHH
FFKHRGIDVQRILGAALTDVKKILTGQKGLHGASTITQQLIKNTVLTNEVSIKRKVKEIY
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LSLDLEDQLSKDQIITAYLNTIPLGGQVYGVEAASLLYFSKNTSDLSLIECAYLAGITQA
EECCCHHHCCCHHEEEEHHHHCCCCCEEEEECEEEEEEEECCCCCHHHHHHHHHHCCCCC
PSYYSAYNENSQKDPSRYINRTLTVLSKMNELGYISESEYNKGVQDINDGKLAFKSSKKD
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEEECCCCC
FRLNYEWFVYPAVSQVKRDLKEKYKYTDEEVSKLIVNGGLKIYTTMDRDLQDFTQKTLDN
EEEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHH
YKNLNVGNSEAYDENKVPLLQASATITDYRNGKVLAMVGGRGKQLPQSNNRAYNDLRSIG
HCCCCCCCCCCCCCCCCCEEEECCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHC
STTKPLTVYGPGIDQEIITAATSIDDAPLSPELTKKYPAYAPPNILRNSPDEYLGLISPR
CCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHCCCCCCCCHHHCCCCHHHEECCCCC
EGIMYSKNTASVITADTIGLKTGISYGENLGLVYNSASKTSIATVALGEFNNDPKDRDGG
CCCEECCCCCEEEEECHHHHHHCCCCCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCC
NTYKVAAAFGSFGNDGLYVEPLLYTKVVDPKGNTVLEGNPKEKQVFSPETAYIMYDLLKG
CEEEEEEEECCCCCCCEEEEEEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHC
PVNFYGSAPAKWSDMPVAGKTGTTTDAKDLWFAGLTPYLSGSVWVGYDNPKTVFGGSGTV
CHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHCCCEEEECCCCCEEECCCCCH
CANLWGKIMAKAHKGMEVKDLEEPSGIVHVAVCKDSGKLPSNLCSSDPRGNRITEELFIE
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCHHHEEC
GTEPHETCNTHISGRINRLNNKLAGANTPALLTQNRVFISKKYPNPLTRDYIYVLPHSQD
CCCCHHHHCCCHHHHHHHHHCHHCCCCCCEEEECCEEEEEECCCCCCCCCEEEEEECCCC
NYIEDQANPELSPPNVNEEGEVEIPNNNGTPQGNNKPPETEQNDSILNSILKPLKPH
CCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STEKTPNKTRKSTNNKRKKKKKKKNKLTASTILKKIGIGVLTLILIVSVVGAGYLLAII
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
KTTPPLDVEDVLSLNQASSLYDSNEEFMDNLHTDEERYVITSDKIPDNLKNAFISIEDER
ECCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCHHHHEEEECHHH
FFKHRGIDVQRILGAALTDVKKILTGQKGLHGASTITQQLIKNTVLTNEVSIKRKVKEIY
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LSLDLEDQLSKDQIITAYLNTIPLGGQVYGVEAASLLYFSKNTSDLSLIECAYLAGITQA
EECCCHHHCCCHHEEEEHHHHCCCCCEEEEECEEEEEEEECCCCCHHHHHHHHHHCCCCC
PSYYSAYNENSQKDPSRYINRTLTVLSKMNELGYISESEYNKGVQDINDGKLAFKSSKKD
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEEECCCCC
FRLNYEWFVYPAVSQVKRDLKEKYKYTDEEVSKLIVNGGLKIYTTMDRDLQDFTQKTLDN
EEEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHH
YKNLNVGNSEAYDENKVPLLQASATITDYRNGKVLAMVGGRGKQLPQSNNRAYNDLRSIG
HCCCCCCCCCCCCCCCCCEEEECCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHC
STTKPLTVYGPGIDQEIITAATSIDDAPLSPELTKKYPAYAPPNILRNSPDEYLGLISPR
CCCCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHCCCCCCCCHHHCCCCHHHEECCCCC
EGIMYSKNTASVITADTIGLKTGISYGENLGLVYNSASKTSIATVALGEFNNDPKDRDGG
CCCEECCCCCEEEEECHHHHHHCCCCCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCC
NTYKVAAAFGSFGNDGLYVEPLLYTKVVDPKGNTVLEGNPKEKQVFSPETAYIMYDLLKG
CEEEEEEEECCCCCCCEEEEEEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHC
PVNFYGSAPAKWSDMPVAGKTGTTTDAKDLWFAGLTPYLSGSVWVGYDNPKTVFGGSGTV
CHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHCCCEEEECCCCCEEECCCCCH
CANLWGKIMAKAHKGMEVKDLEEPSGIVHVAVCKDSGKLPSNLCSSDPRGNRITEELFIE
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCHHHEEC
GTEPHETCNTHISGRINRLNNKLAGANTPALLTQNRVFISKKYPNPLTRDYIYVLPHSQD
CCCCHHHHCCCHHHHHHHHHCHHCCCCCCEEEECCEEEEEECCCCCCCCCEEEEEECCCC
NYIEDQANPELSPPNVNEEGEVEIPNNNGTPQGNNKPPETEQNDSILNSILKPLKPH
CCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA