| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is pbpE [H]
Identifier: 187935100
GI number: 187935100
Start: 3089600
End: 3091705
Strand: Reverse
Name: pbpE [H]
Synonym: CLL_A2995
Alternate gene names: 187935100
Gene position: 3091705-3089600 (Counterclockwise)
Preceding gene: 187933613
Following gene: 187934168
Centisome position: 81.35
GC content: 28.16
Gene sequence:
>2106_bases ATGAAAAGAAAAATTATCGCAGGGTTATTTTTAACATTAATCTTTTCAGTTACTAGTTCTATACAAGTTTTAGCTATTGA AAAAGATTCAACATCTAATTTAAATGTAACTAGCACCAATAAAACTGATGAGAATTTATCAGTTGATGAGAGAATAAAAC AGCAGGCGAATTTACTGGTTGATAATTATGATACAACAAGTGTTCAATATGCTGTTATTGATTCTGGTAAAATTATATTA TCAGATAATGCAGGTGTTTATAGTAGAAATGAAAATAGAGAACTTACTAAAAATACAATGTACGGTATTGGATCTATAAG TAAGATGTTTGTTGTAGCATCTGTTATGAAATTAGTAGATGAAAATAAAATTGATTTAGACAAACCAGTAGTTAATTATA TACCAGATTTTAAAATGCAAGATGAACGCTATAAACAAATTACTCCAAGGATGCTTATAAATCATTCTTCAGGTATCATG GGAACAACTTTTAAGAATACATTTCTATTTGATGATAATGATACAACAGGTTATGATCAACTTCTTTCTGAACTTGCAAA TCAAAGATTAAAAGCTAATCCAGGTGAATATTCTGTTTATTGTAATGATGGTTTTACACTTGCACAATTACTTGTTGAAC GTGTTAGTGGTACTGATTTTACAACATATATACATGAAAATTTTACCAAGCCTTTAAATATTACTAATACAAAAACTCCA CTTAATAGTTTTGATAAGAGAAGTTTAGCTAAAGTCTATGATAATGAATCAGAGAAAAAAGAATTACCTAATGAAAGTGT AAATATTATTGGTACTGGAGGTATATATTCAACAGCAGAAGATCTTTGTCAATTTTCACGTATTTTTACTAATGACGATA TAAAGATTTTATCAAAAAACTCTATAGATGCAATGTCTGAAAAAGAATATGCTAAAGGCATACATATTAAAGCGGAAGAT AATACTATAGGGTATGGTCTTGGATGGGACAATGTAGATACCTTCCCATTTAATAGATATGGAATAAAAGCATTGACTAA AGGTGGAGATACATTTGCTTATCATGGTAGTGTAATTTCTTTACCACAATATGACTTATCAGTAGCAGTTGTTTCTTCAG GGGGGACAAGTGGCTATGATCAAGCTTTTGGTACAAGTGTTTTATTACAAATTCTTAAGGAAAAAGGTATTATTTCTGAA ATACTTCCAGATGTAACTTTACCAAATGCTAAAGTAGCAGATATGCCAAAAGAATTTACTAAATATTCAGGATTATATAA TATATCAAATACAATGGCAAAAGTTGATATTAATTCTAATGGAACTTTAACAATTAATCTTATAGGTGGTTCAAAGGATA GTGAGCAAGTTTACACTTATACTGATGATGGATATTTTACTTCAGCAGATGGAAGAGGTAAATGCAATTTTGTTGAAGAA AAGAATGGGATTACTTATTTAGAAAGAAAATTATATATGAGTTTGGATGGTTTATCACAAAGTGTTGTTTGGGATTATAA TTTACAAAAGGTAGAAGAGAATCCTATTAGTGATGATTTAAAATCAGTGTGGAAATTACGAAATAATAAAGACTATTATG TATTAAATGAAAAATATACATCTGAAAAATATATGAAGTTTTTAAATGCAATCCATATGAATTTTAAGGATGAAGATCAA GGCTATTTTATGGGGCATAAAATAGTAAGTGAAAATGAAGCAAAAGCTATTACTCAAATACCAGGTACTGGAGGACGTGA TTTAGTTGACTTTAAATTCTATAAAGAAAATGATATAGAATATCTTAAATTTGGTTCTAGTATTGCAATAAGCTCTGATG CAATTCCAATATTAAGTAAGGATTTAAATAAAGTTACAATTGGAGAAGATGGATATGCAAAGTGGTATACTGTTCCAACT GAAATGTCTGGAAAAATTATCAATGTATCTCAACCTGAAAATGGTGCATTTGCTGTTTATGATGCAAATGGTAAATATAT CAATTATAGTTATATAAGTGGAGAAAATAATATTTCATTAGATAATGGATATCATATTGTCTTTATGGGTGATAAAGGTG GAACATTTAATATTAATTTTAAATAA
Upstream 100 bases:
>100_bases AAACTATATCTTAACGTAAACAAATTTTAAAATAAATTCCATAATTGTAAAATGATTATTTAACTATGAATTTATTTAAG GTTATAGGGGGAATTATAAA
Downstream 100 bases:
>100_bases ATAATGCTAAAATAGTAGAAATAAATAACTTTAAAAGATAATTATAAACTTATAGTGCATAGTTAAATGAAAAATAATTA CCATTTAATAAAAGTATTTG
Product: cell surface protein
Products: NA
Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]
Number of amino acids: Translated: 701; Mature: 701
Protein sequence:
>701_residues MKRKIIAGLFLTLIFSVTSSIQVLAIEKDSTSNLNVTSTNKTDENLSVDERIKQQANLLVDNYDTTSVQYAVIDSGKIIL SDNAGVYSRNENRELTKNTMYGIGSISKMFVVASVMKLVDENKIDLDKPVVNYIPDFKMQDERYKQITPRMLINHSSGIM GTTFKNTFLFDDNDTTGYDQLLSELANQRLKANPGEYSVYCNDGFTLAQLLVERVSGTDFTTYIHENFTKPLNITNTKTP LNSFDKRSLAKVYDNESEKKELPNESVNIIGTGGIYSTAEDLCQFSRIFTNDDIKILSKNSIDAMSEKEYAKGIHIKAED NTIGYGLGWDNVDTFPFNRYGIKALTKGGDTFAYHGSVISLPQYDLSVAVVSSGGTSGYDQAFGTSVLLQILKEKGIISE ILPDVTLPNAKVADMPKEFTKYSGLYNISNTMAKVDINSNGTLTINLIGGSKDSEQVYTYTDDGYFTSADGRGKCNFVEE KNGITYLERKLYMSLDGLSQSVVWDYNLQKVEENPISDDLKSVWKLRNNKDYYVLNEKYTSEKYMKFLNAIHMNFKDEDQ GYFMGHKIVSENEAKAITQIPGTGGRDLVDFKFYKENDIEYLKFGSSIAISSDAIPILSKDLNKVTIGEDGYAKWYTVPT EMSGKIINVSQPENGAFAVYDANGKYINYSYISGENNISLDNGYHIVFMGDKGGTFNINFK
Sequences:
>Translated_701_residues MKRKIIAGLFLTLIFSVTSSIQVLAIEKDSTSNLNVTSTNKTDENLSVDERIKQQANLLVDNYDTTSVQYAVIDSGKIIL SDNAGVYSRNENRELTKNTMYGIGSISKMFVVASVMKLVDENKIDLDKPVVNYIPDFKMQDERYKQITPRMLINHSSGIM GTTFKNTFLFDDNDTTGYDQLLSELANQRLKANPGEYSVYCNDGFTLAQLLVERVSGTDFTTYIHENFTKPLNITNTKTP LNSFDKRSLAKVYDNESEKKELPNESVNIIGTGGIYSTAEDLCQFSRIFTNDDIKILSKNSIDAMSEKEYAKGIHIKAED NTIGYGLGWDNVDTFPFNRYGIKALTKGGDTFAYHGSVISLPQYDLSVAVVSSGGTSGYDQAFGTSVLLQILKEKGIISE ILPDVTLPNAKVADMPKEFTKYSGLYNISNTMAKVDINSNGTLTINLIGGSKDSEQVYTYTDDGYFTSADGRGKCNFVEE KNGITYLERKLYMSLDGLSQSVVWDYNLQKVEENPISDDLKSVWKLRNNKDYYVLNEKYTSEKYMKFLNAIHMNFKDEDQ GYFMGHKIVSENEAKAITQIPGTGGRDLVDFKFYKENDIEYLKFGSSIAISSDAIPILSKDLNKVTIGEDGYAKWYTVPT EMSGKIINVSQPENGAFAVYDANGKYINYSYISGENNISLDNGYHIVFMGDKGGTFNINFK >Mature_701_residues MKRKIIAGLFLTLIFSVTSSIQVLAIEKDSTSNLNVTSTNKTDENLSVDERIKQQANLLVDNYDTTSVQYAVIDSGKIIL SDNAGVYSRNENRELTKNTMYGIGSISKMFVVASVMKLVDENKIDLDKPVVNYIPDFKMQDERYKQITPRMLINHSSGIM GTTFKNTFLFDDNDTTGYDQLLSELANQRLKANPGEYSVYCNDGFTLAQLLVERVSGTDFTTYIHENFTKPLNITNTKTP LNSFDKRSLAKVYDNESEKKELPNESVNIIGTGGIYSTAEDLCQFSRIFTNDDIKILSKNSIDAMSEKEYAKGIHIKAED NTIGYGLGWDNVDTFPFNRYGIKALTKGGDTFAYHGSVISLPQYDLSVAVVSSGGTSGYDQAFGTSVLLQILKEKGIISE ILPDVTLPNAKVADMPKEFTKYSGLYNISNTMAKVDINSNGTLTINLIGGSKDSEQVYTYTDDGYFTSADGRGKCNFVEE KNGITYLERKLYMSLDGLSQSVVWDYNLQKVEENPISDDLKSVWKLRNNKDYYVLNEKYTSEKYMKFLNAIHMNFKDEDQ GYFMGHKIVSENEAKAITQIPGTGGRDLVDFKFYKENDIEYLKFGSSIAISSDAIPILSKDLNKVTIGEDGYAKWYTVPT EMSGKIINVSQPENGAFAVYDANGKYINYSYISGENNISLDNGYHIVFMGDKGGTFNINFK
Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]
COG id: COG1680
COG function: function code V; Beta-lactamase class C and other penicillin binding proteins
Gene ontology:
Cell location: Cell membrane; Peripheral membrane protein [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the beta-lactamase family [H]
Homologues:
Organism=Escherichia coli, GI1786574, Length=283, Percent_Identity=26.5017667844523, Blast_Score=78, Evalue=2e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001466 - InterPro: IPR021860 [H]
Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]
EC number: NA
Molecular weight: Translated: 78449; Mature: 78449
Theoretical pI: Translated: 4.81; Mature: 4.81
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKRKIIAGLFLTLIFSVTSSIQVLAIEKDSTSNLNVTSTNKTDENLSVDERIKQQANLLV CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCEEE DNYDTTSVQYAVIDSGKIILSDNAGVYSRNENRELTKNTMYGIGSISKMFVVASVMKLVD ECCCCCEEEEEEEECCEEEEECCCCCEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHC ENKIDLDKPVVNYIPDFKMQDERYKQITPRMLINHSSGIMGTTFKNTFLFDDNDTTGYDQ CCCCCCCCCHHHHCCCCCCCCHHHHHCCCEEEEECCCCCEEEEECCEEEEECCCCCCHHH LLSELANQRLKANPGEYSVYCNDGFTLAQLLVERVSGTDFTTYIHENFTKPLNITNTKTP HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCCCC LNSFDKRSLAKVYDNESEKKELPNESVNIIGTGGIYSTAEDLCQFSRIFTNDDIKILSKN CCHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECC SIDAMSEKEYAKGIHIKAEDNTIGYGLGWDNVDTFPFNRYGIKALTKGGDTFAYHGSVIS CCCHHCHHHHHCCEEEEECCCEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEECCCEEE LPQYDLSVAVVSSGGTSGYDQAFGTSVLLQILKEKGIISEILPDVTLPNAKVADMPKEFT CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHCCCHHHH KYSGLYNISNTMAKVDINSNGTLTINLIGGSKDSEQVYTYTDDGYFTSADGRGKCNFVEE HHCCEEECCCCEEEEEECCCCEEEEEEECCCCCCCEEEEEECCCEEECCCCCCCEEEEEC KNGITYLERKLYMSLDGLSQSVVWDYNLQKVEENPISDDLKSVWKLRNNKDYYVLNEKYT CCCCEEEEHEEHEEECCCCCEEEEECCHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCC SEKYMKFLNAIHMNFKDEDQGYFMGHKIVSENEAKAITQIPGTGGRDLVDFKFYKENDIE HHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCEEEEEEECCCCCE YLKFGSSIAISSDAIPILSKDLNKVTIGEDGYAKWYTVPTEMSGKIINVSQPENGAFAVY EEECCCEEEECCCCCCHHHCCCCEEEECCCCCEEEEECCCCCCCEEEEECCCCCCEEEEE DANGKYINYSYISGENNISLDNGYHIVFMGDKGGTFNINFK CCCCCEEEEEEECCCCCEEECCCEEEEEECCCCCEEEEEEC >Mature Secondary Structure MKRKIIAGLFLTLIFSVTSSIQVLAIEKDSTSNLNVTSTNKTDENLSVDERIKQQANLLV CCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCEEE DNYDTTSVQYAVIDSGKIILSDNAGVYSRNENRELTKNTMYGIGSISKMFVVASVMKLVD ECCCCCEEEEEEEECCEEEEECCCCCEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHC ENKIDLDKPVVNYIPDFKMQDERYKQITPRMLINHSSGIMGTTFKNTFLFDDNDTTGYDQ CCCCCCCCCHHHHCCCCCCCCHHHHHCCCEEEEECCCCCEEEEECCEEEEECCCCCCHHH LLSELANQRLKANPGEYSVYCNDGFTLAQLLVERVSGTDFTTYIHENFTKPLNITNTKTP HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCCCC LNSFDKRSLAKVYDNESEKKELPNESVNIIGTGGIYSTAEDLCQFSRIFTNDDIKILSKN CCHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECC SIDAMSEKEYAKGIHIKAEDNTIGYGLGWDNVDTFPFNRYGIKALTKGGDTFAYHGSVIS CCCHHCHHHHHCCEEEEECCCEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEECCCEEE LPQYDLSVAVVSSGGTSGYDQAFGTSVLLQILKEKGIISEILPDVTLPNAKVADMPKEFT CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHCCCHHHH KYSGLYNISNTMAKVDINSNGTLTINLIGGSKDSEQVYTYTDDGYFTSADGRGKCNFVEE HHCCEEECCCCEEEEEECCCCEEEEEEECCCCCCCEEEEEECCCEEECCCCCCCEEEEEC KNGITYLERKLYMSLDGLSQSVVWDYNLQKVEENPISDDLKSVWKLRNNKDYYVLNEKYT CCCCEEEEHEEHEEECCCCCEEEEECCHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCC SEKYMKFLNAIHMNFKDEDQGYFMGHKIVSENEAKAITQIPGTGGRDLVDFKFYKENDIE HHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCEEEEEEECCCCCE YLKFGSSIAISSDAIPILSKDLNKVTIGEDGYAKWYTVPTEMSGKIINVSQPENGAFAVY EEECCCEEEECCCCCCHHHCCCCEEEECCCCCEEEEECCCCCCCEEEEECCCCCCEEEEE DANGKYINYSYISGENNISLDNGYHIVFMGDKGGTFNINFK CCCCCEEEEEEECCCCCEEECCCEEEEEECCCCCEEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 8491712; 9384377 [H]