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The map label for this gene is mscL [H]

Identifier: 187935041

GI number: 187935041

Start: 2125802

End: 2126218

Strand: Reverse

Name: mscL [H]

Synonym: CLL_A2031

Alternate gene names: 187935041

Gene position: 2126218-2125802 (Counterclockwise)

Preceding gene: 187933891

Following gene: 187932540

Centisome position: 55.95

GC content: 26.38

Gene sequence:

>417_bases
ATGAAGAAATTTTTAAATGAATTTAAACAGTTTGCATTAAAGGGAAGTGTAATTGACTTAGCAGTTGGTGTATTAATAGG
TGGTGCATTTCAAGGAATAGTAAAATCATTAACAAATGATATAATATCACCTATAATAGGAATATTTGCAAATAAAGATT
TCAATGATTTAACATTAGAACTTTTTGGTGTAAATATTAAATATGGATCTTTCATAACGGCAGTTATAAATTTTTTAATT
ATGGCATTTTTAATATTTTTACTTGTTAAAGGTATGAATAAATTAAGTGAATTAGGAAGAAGAAACAAAGTTGAGGAAGT
GAAAGAGCCTACAATTAAAGAATGTCCTTTTTGCTACAGTGAAATTGATATAAAAGCAACTCGTTGCCCAAAATGTACAT
CTCAGTTAGAAGTATAA

Upstream 100 bases:

>100_bases
TAGTTTTTATGACTAAAAACATATAAATGTGATAAAATATCAAGGTATATAGATATTAGAAAAACAGAGGAAAAAACCAA
GGAGGAAAAAAGAAAAGTTT

Downstream 100 bases:

>100_bases
AAATCAATAAAGAAGAGAAATATATTATGAATATTTAGATAAAATACTATATGATTAATTTGAACAAATAGTATATAAAT
AAAAGACATCAGTACTAAAA

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MKKFLNEFKQFALKGSVIDLAVGVLIGGAFQGIVKSLTNDIISPIIGIFANKDFNDLTLELFGVNIKYGSFITAVINFLI
MAFLIFLLVKGMNKLSELGRRNKVEEVKEPTIKECPFCYSEIDIKATRCPKCTSQLEV

Sequences:

>Translated_138_residues
MKKFLNEFKQFALKGSVIDLAVGVLIGGAFQGIVKSLTNDIISPIIGIFANKDFNDLTLELFGVNIKYGSFITAVINFLI
MAFLIFLLVKGMNKLSELGRRNKVEEVKEPTIKECPFCYSEIDIKATRCPKCTSQLEV
>Mature_138_residues
MKKFLNEFKQFALKGSVIDLAVGVLIGGAFQGIVKSLTNDIISPIIGIFANKDFNDLTLELFGVNIKYGSFITAVINFLI
MAFLIFLLVKGMNKLSELGRRNKVEEVKEPTIKECPFCYSEIDIKATRCPKCTSQLEV

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=126, Percent_Identity=40.4761904761905, Blast_Score=80, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15387; Mature: 15387

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFLNEFKQFALKGSVIDLAVGVLIGGAFQGIVKSLTNDIISPIIGIFANKDFNDLTLE
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LFGVNIKYGSFITAVINFLIMAFLIFLLVKGMNKLSELGRRNKVEEVKEPTIKECPFCYS
HEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHCCCCHH
EIDIKATRCPKCTSQLEV
HCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MKKFLNEFKQFALKGSVIDLAVGVLIGGAFQGIVKSLTNDIISPIIGIFANKDFNDLTLE
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LFGVNIKYGSFITAVINFLIMAFLIFLLVKGMNKLSELGRRNKVEEVKEPTIKECPFCYS
HEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHCCCCHH
EIDIKATRCPKCTSQLEV
HCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA