| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is cwlL [H]
Identifier: 187934789
GI number: 187934789
Start: 3277275
End: 3279653
Strand: Reverse
Name: cwlL [H]
Synonym: CLL_A3162
Alternate gene names: 187934789
Gene position: 3279653-3277275 (Counterclockwise)
Preceding gene: 187935538
Following gene: 187932451
Centisome position: 86.3
GC content: 29.09
Gene sequence:
>2379_bases TTGGGAAAGGGATATATATTAGTAAGACTATTTAAGTTTAAGGATGCTTTGCCTAATACAAAAGGAAAACTTATAATTAC TAATGCTGAAAATGGAGAAATAGTATATGAGAAAGATGATGCTTTTGATATTAGTGGAAGAAGTGAATTTATAGAAGTAA TAACACCAGAAAAAGGTTTATCTCAACAACCATATGAAGAAGGAGTTATTCCATATGGTATATATAATATAGATATATTG CCTGAAAATTTTGAAGAAGTTATTATTGACGGTGTAAGTGTTTTTGAAGATATTACTTCTATACAAAATGTCGAAGTTGG TGAAGAAGAAGAGAATAATTACAATAATGGTTTACCTCAAAAAATTATTATTCCACCAGAACAGCTTGTACTTAACGCAA AAAGAGATAAACAAGTAGGTTCTATGGTTCAGCCTTTAGTTTTAGAAAGGCCATATATACCTGAATTTATTGTTGTACAT TTAGGTCCACCAAGTGTACCTGCTGAGAATGTAACAGTAGGATTTGTAGATTATATTAAAAATGTTGCTTCAAGTGAAAT ATACCCAACATGGCCTGAAGAATCATTAAAAGCCAATATATATTGCCAAATATCATTCGCATTAAATAGAATTTATACAG AATGGTATAGAGGTAAGGGATATTCATTTCAAATTACTAATTCACCTGCATATGATCAATATTTTATAAAGGGTAGAAAT ATATTTGATAATATAAGTAAAATAGTAGACGAAATTTTCAGTGAATATATAGTAACTATTGGTAATAGAACACCACTTTT TGCACAATATTGCAATGGTACAACAGTTAAGTGTGATGGATTATCACAATGGGGAAGTGTAGATTTAGCCAATGCAGGTA TGCAAGCTTTAGATATTATTAGATATTATTTTGGTTATGATAAAGATTTAGTTAGAGCAACTATGATTGAAGGAATACCA GAATCTTATCCGGGAACACCTCTTAGACTTAATGATGAAAATAATAATGTTAAGGTTATACAAAAACAATTAAATAGGAT ATCTCAAAATTTCCCTGCAATACCTAAAATACCATATGAGAATGGAAAGTTTGATAAATCAACTGAAGATGCAGTAAAGG TGTTTCAGAAAGTTTTTAATCTTACCCAAGATGGAATTGTAGGAAGAGCTACGTGGTATAGAATATCATCTATTTATGTT GGTGTAAAAAGATTAGCAGAGCTTGATCAAGAGCCAGAAATTGATGGAGAAAATCCACCTCCAGGGGGAGAGTATCCAGG ATATTTATTAAAATATGGTTCAAGGGGAGAAAAAGTTAAAGAAGTTCAGAGTTATCTTTCAGTTATATCTAAATCGTATA ATATACCAAGTATTAAGGCAGATGGAATATTTGGTCAAATGACAAAAGATGCTGTTATAGCTTTTCAAAGGTTATTTGGA TTAGCTCCAGATGGAATAATAGGAATAAATACTTGGAATAAAATATATTATGTATATAAAGATTTGATTAAAGGAACTGG ACAGCAAGACCTTAATGAAGACTTTGATGGAAAATATCCAGGTTATTTATTAAGTTATGGCTCAAGAGGAGAAAAAGTTA GAGAAATGCAGACATATCTTTCAGTTATATCTAAATCATATAATATACCAAGTATTAATGCAGATGGAATATTTGGAGAG ATGACAAGAAATGCTGTATTATCTTTTCAAAGATTATTTGGGTTAGCTCAAGATGGGGTAGTAGGATTAAATACTTGGAA TAAGATATATGAAATTTATAGAGGTTCAGAAAAGTCTAATGTAGAAGAAAAGATTTATTCAGCTGACTATCCAGGATATG TGTTAAAAGAAAATTTATATGGCGATGATGTTAGATGGTTACAAACTTATTTAAATGCCATATCTGAGTTTTATAAAGAG ATACCTAAGATAAAAGTAGATGGAATGTTTAAGAAAAAAACTAAAAGTGCTGTAATGGAATTTCAAAAAAAATTTGGATT GAATGTAGATGGAAAAATTGGCGTAAATGATTGGAAAAAATTAATATCAGTATACAATAGTTTAGATGGTGGACAAAATA TTAATAAATCTGATATATTATATGATTATCCAGGTTTTGACTTAGAATTAGGAGATCGAGATGGATATGTCACAGTTTTT CAAAAATATATAAATGTTCTTGCTAAAAATAATTATTTATCAAATCAAATTATAGAAAATGGTGTATTTGATAAAAGAAC AGAAAAGGCTATTAAAGAACTACAGGAGAAGTTTGGATTAAAAGTAACGGGAATAGTTGATAAACTTACTTGGGATAAAA CAGCATCATTATATGAATTTTTATATACTGGTAAAGGAAGTAAAAGAAATAGAAAGTAA
Upstream 100 bases:
>100_bases ATAATTCTCAGAGTTATTTTAAATTATAGTCATTAAGAACCATTTAGATATCAGATGTATAAGTTAATATAAGTATTAAT AATCTTATAGGGAGAACATT
Downstream 100 bases:
>100_bases GAATTTTTAAAATATACTTTTTGAAATTTTGAATTTATGTTAATAATAAAAAGCATAAAAAAGATGTAAATATATCATAC ATTTTTTTATGCTTTTATTG
Product: spore cortex-lytic enzyme, pre-pro-form
Products: NA
Alternate protein names: Autolysin; Cell wall hydrolase [H]
Number of amino acids: Translated: 792; Mature: 791
Protein sequence:
>792_residues MGKGYILVRLFKFKDALPNTKGKLIITNAENGEIVYEKDDAFDISGRSEFIEVITPEKGLSQQPYEEGVIPYGIYNIDIL PENFEEVIIDGVSVFEDITSIQNVEVGEEEENNYNNGLPQKIIIPPEQLVLNAKRDKQVGSMVQPLVLERPYIPEFIVVH LGPPSVPAENVTVGFVDYIKNVASSEIYPTWPEESLKANIYCQISFALNRIYTEWYRGKGYSFQITNSPAYDQYFIKGRN IFDNISKIVDEIFSEYIVTIGNRTPLFAQYCNGTTVKCDGLSQWGSVDLANAGMQALDIIRYYFGYDKDLVRATMIEGIP ESYPGTPLRLNDENNNVKVIQKQLNRISQNFPAIPKIPYENGKFDKSTEDAVKVFQKVFNLTQDGIVGRATWYRISSIYV GVKRLAELDQEPEIDGENPPPGGEYPGYLLKYGSRGEKVKEVQSYLSVISKSYNIPSIKADGIFGQMTKDAVIAFQRLFG LAPDGIIGINTWNKIYYVYKDLIKGTGQQDLNEDFDGKYPGYLLSYGSRGEKVREMQTYLSVISKSYNIPSINADGIFGE MTRNAVLSFQRLFGLAQDGVVGLNTWNKIYEIYRGSEKSNVEEKIYSADYPGYVLKENLYGDDVRWLQTYLNAISEFYKE IPKIKVDGMFKKKTKSAVMEFQKKFGLNVDGKIGVNDWKKLISVYNSLDGGQNINKSDILYDYPGFDLELGDRDGYVTVF QKYINVLAKNNYLSNQIIENGVFDKRTEKAIKELQEKFGLKVTGIVDKLTWDKTASLYEFLYTGKGSKRNRK
Sequences:
>Translated_792_residues MGKGYILVRLFKFKDALPNTKGKLIITNAENGEIVYEKDDAFDISGRSEFIEVITPEKGLSQQPYEEGVIPYGIYNIDIL PENFEEVIIDGVSVFEDITSIQNVEVGEEEENNYNNGLPQKIIIPPEQLVLNAKRDKQVGSMVQPLVLERPYIPEFIVVH LGPPSVPAENVTVGFVDYIKNVASSEIYPTWPEESLKANIYCQISFALNRIYTEWYRGKGYSFQITNSPAYDQYFIKGRN IFDNISKIVDEIFSEYIVTIGNRTPLFAQYCNGTTVKCDGLSQWGSVDLANAGMQALDIIRYYFGYDKDLVRATMIEGIP ESYPGTPLRLNDENNNVKVIQKQLNRISQNFPAIPKIPYENGKFDKSTEDAVKVFQKVFNLTQDGIVGRATWYRISSIYV GVKRLAELDQEPEIDGENPPPGGEYPGYLLKYGSRGEKVKEVQSYLSVISKSYNIPSIKADGIFGQMTKDAVIAFQRLFG LAPDGIIGINTWNKIYYVYKDLIKGTGQQDLNEDFDGKYPGYLLSYGSRGEKVREMQTYLSVISKSYNIPSINADGIFGE MTRNAVLSFQRLFGLAQDGVVGLNTWNKIYEIYRGSEKSNVEEKIYSADYPGYVLKENLYGDDVRWLQTYLNAISEFYKE IPKIKVDGMFKKKTKSAVMEFQKKFGLNVDGKIGVNDWKKLISVYNSLDGGQNINKSDILYDYPGFDLELGDRDGYVTVF QKYINVLAKNNYLSNQIIENGVFDKRTEKAIKELQEKFGLKVTGIVDKLTWDKTASLYEFLYTGKGSKRNRK >Mature_791_residues GKGYILVRLFKFKDALPNTKGKLIITNAENGEIVYEKDDAFDISGRSEFIEVITPEKGLSQQPYEEGVIPYGIYNIDILP ENFEEVIIDGVSVFEDITSIQNVEVGEEEENNYNNGLPQKIIIPPEQLVLNAKRDKQVGSMVQPLVLERPYIPEFIVVHL GPPSVPAENVTVGFVDYIKNVASSEIYPTWPEESLKANIYCQISFALNRIYTEWYRGKGYSFQITNSPAYDQYFIKGRNI FDNISKIVDEIFSEYIVTIGNRTPLFAQYCNGTTVKCDGLSQWGSVDLANAGMQALDIIRYYFGYDKDLVRATMIEGIPE SYPGTPLRLNDENNNVKVIQKQLNRISQNFPAIPKIPYENGKFDKSTEDAVKVFQKVFNLTQDGIVGRATWYRISSIYVG VKRLAELDQEPEIDGENPPPGGEYPGYLLKYGSRGEKVKEVQSYLSVISKSYNIPSIKADGIFGQMTKDAVIAFQRLFGL APDGIIGINTWNKIYYVYKDLIKGTGQQDLNEDFDGKYPGYLLSYGSRGEKVREMQTYLSVISKSYNIPSINADGIFGEM TRNAVLSFQRLFGLAQDGVVGLNTWNKIYEIYRGSEKSNVEEKIYSADYPGYVLKENLYGDDVRWLQTYLNAISEFYKEI PKIKVDGMFKKKTKSAVMEFQKKFGLNVDGKIGVNDWKKLISVYNSLDGGQNINKSDILYDYPGFDLELGDRDGYVTVFQ KYINVLAKNNYLSNQIIENGVFDKRTEKAIKELQEKFGLKVTGIVDKLTWDKTASLYEFLYTGKGSKRNRK
Specific function: Unknown
COG id: NA
COG function: NA
Gene ontology:
Cell location: Secreted (Potential) [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002502 - InterPro: IPR002477 [H]
Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 89923; Mature: 89792
Theoretical pI: Translated: 4.99; Mature: 4.99
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 1.5 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.0 %Met (Mature Protein) 1.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGKGYILVRLFKFKDALPNTKGKLIITNAENGEIVYEKDDAFDISGRSEFIEVITPEKGL CCCCEEEEEEEHHHHCCCCCCCCEEEEECCCCCEEEECCCCEECCCCHHEEEEECCCCCC SQQPYEEGVIPYGIYNIDILPENFEEVIIDGVSVFEDITSIQNVEVGEEEENNYNNGLPQ CCCCHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC KIIIPPEQLVLNAKRDKQVGSMVQPLVLERPYIPEFIVVHLGPPSVPAENVTVGFVDYIK EEEECHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEHHHHHHH NVASSEIYPTWPEESLKANIYCQISFALNRIYTEWYRGKGYSFQITNSPAYDQYFIKGRN HHHCCCCCCCCCCHHCCEEEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCH IFDNISKIVDEIFSEYIVTIGNRTPLFAQYCNGTTVKCDGLSQWGSVDLANAGMQALDII HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEECCCCCCCCCCHHHCCHHHHHHH RYYFGYDKDLVRATMIEGIPESYPGTPLRLNDENNNVKVIQKQLNRISQNFPAIPKIPYE HHHHCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC NGKFDKSTEDAVKVFQKVFNLTQDGIVGRATWYRISSIYVGVKRLAELDQEPEIDGENPP CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC PGGEYPGYLLKYGSRGEKVKEVQSYLSVISKSYNIPSIKADGIFGQMTKDAVIAFQRLFG CCCCCCCHHEEECCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHC LAPDGIIGINTWNKIYYVYKDLIKGTGQQDLNEDFDGKYPGYLLSYGSRGEKVREMQTYL CCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHH SVISKSYNIPSINADGIFGEMTRNAVLSFQRLFGLAQDGVVGLNTWNKIYEIYRGSEKSN HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCCCCCC VEEKIYSADYPGYVLKENLYGDDVRWLQTYLNAISEFYKEIPKIKVDGMFKKKTKSAVME HHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHH FQKKFGLNVDGKIGVNDWKKLISVYNSLDGGQNINKSDILYDYPGFDLELGDRDGYVTVF HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEECCCCCHHHHH QKYINVLAKNNYLSNQIIENGVFDKRTEKAIKELQEKFGLKVTGIVDKLTWDKTASLYEF HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHH LYTGKGSKRNRK HHCCCCCCCCCC >Mature Secondary Structure GKGYILVRLFKFKDALPNTKGKLIITNAENGEIVYEKDDAFDISGRSEFIEVITPEKGL CCCEEEEEEEHHHHCCCCCCCCEEEEECCCCCEEEECCCCEECCCCHHEEEEECCCCCC SQQPYEEGVIPYGIYNIDILPENFEEVIIDGVSVFEDITSIQNVEVGEEEENNYNNGLPQ CCCCHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC KIIIPPEQLVLNAKRDKQVGSMVQPLVLERPYIPEFIVVHLGPPSVPAENVTVGFVDYIK EEEECHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEHHHHHHH NVASSEIYPTWPEESLKANIYCQISFALNRIYTEWYRGKGYSFQITNSPAYDQYFIKGRN HHHCCCCCCCCCCHHCCEEEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCH IFDNISKIVDEIFSEYIVTIGNRTPLFAQYCNGTTVKCDGLSQWGSVDLANAGMQALDII HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEECCCCCCCCCCHHHCCHHHHHHH RYYFGYDKDLVRATMIEGIPESYPGTPLRLNDENNNVKVIQKQLNRISQNFPAIPKIPYE HHHHCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC NGKFDKSTEDAVKVFQKVFNLTQDGIVGRATWYRISSIYVGVKRLAELDQEPEIDGENPP CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC PGGEYPGYLLKYGSRGEKVKEVQSYLSVISKSYNIPSIKADGIFGQMTKDAVIAFQRLFG CCCCCCCHHEEECCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHC LAPDGIIGINTWNKIYYVYKDLIKGTGQQDLNEDFDGKYPGYLLSYGSRGEKVREMQTYL CCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHH SVISKSYNIPSINADGIFGEMTRNAVLSFQRLFGLAQDGVVGLNTWNKIYEIYRGSEKSN HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCCCCCC VEEKIYSADYPGYVLKENLYGDDVRWLQTYLNAISEFYKEIPKIKVDGMFKKKTKSAVME HHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHH FQKKFGLNVDGKIGVNDWKKLISVYNSLDGGQNINKSDILYDYPGFDLELGDRDGYVTVF HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEECCCCCHHHHH QKYINVLAKNNYLSNQIIENGVFDKRTEKAIKELQEKFGLKVTGIVDKLTWDKTASLYEF HHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHH LYTGKGSKRNRK HHCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7902527 [H]