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The map label for this gene is metH [H]

Identifier: 187934679

GI number: 187934679

Start: 2927182

End: 2929581

Strand: Reverse

Name: metH [H]

Synonym: CLL_A2853

Alternate gene names: 187934679

Gene position: 2929581-2927182 (Counterclockwise)

Preceding gene: 187935688

Following gene: 187935058

Centisome position: 77.09

GC content: 30.21

Gene sequence:

>2400_bases
GTGGGGCTTAAAGAATTTATAAAAGATAATATACTTGTATTTGATGGGGCAATGGGAACAATGCTTCAAAAAGAGGGACT
AAAACTTGGAGAAAATCCTGAAATATTTAATATAGAGGAAAGTGAAAAAGTAAAGAAAATTCATGAGGAATATATTAAAA
ATGGAGCCATGATTATAACATCAAATACTTTTGGGGCAAATGAATTGAAATTAGAAAATACAGGATATACAGTTGAAGAA
ATTATAAATGCAGCAATTTCTCTAGCAAAAGAAGCTACAGGAAATGAGAAAGTCTATATTGCATTAGATATAGGACCAAT
AGGCGAACTTTTAGAACCAATGGGTACATTAAGTTTTGATAGAGCTTATGAGATATTTAAAAGGCAAATAGTGCAAGGTG
AAAAATCTGGTGCAGATTTAATTTTAATTGAAACAATGACAGATCTTTACGAAGCAAAAGCAGCTCTTTTGGCAGCAAAA
GAAAATACTAGTTTACCTGTATTTTGTACTATGTCATTTGATGAAAATGGAAGGACTTTTACAGGATGTACACCAGAAAG
TATGGCAATCACTCTGGAGGGCATAGGAGCAGATGCTGTAGGAATAAATTGCTCTTTAGGACCTAAAGAATTACTTCCAA
TAGTAAAAAGAATAAAGAAAGCAACTAATTTACCTATAATGACACAACCTAATGCTGGTTTACCTAAGCTATCTTTTGGT
GAAGCAATATATGACATTACTTCAGACGAATTTGAATATTGGGTAAATGAATTTGTTAAAGAAGGCGTAAGTATAATTGG
AGGATGTTGTGGAACAACTCCAAAATTTATCAAAAGGCTAAGAGTACTTGCAGACAATAATAAAAGAATAATAAGAGATA
AAATTGAATTTTCAGCTGTATGTACACCATCAAATGTTGTCAAAATTGAAGGTGTAAAGGTAATTGGAGAAAGAATAAAT
CCAACAGGAAAGAAGTTATTTAAAGAAGCACTTAAAAACAATGATATTGATTATATATTAAAACAAGCAATAGAACAAAT
TGAAGCTGGTGCTGAGATACTTGACGTAAATGTAGGACTACCTGAAATAAATGAAAAAGAAATGATGAAGAAAGTAGTTA
AAGAGATTCAGGGGATTATAGATGCGCCATTACAAATAGATTCATCAAACATAGCAGCTATTGAGACAGGATTAAGAGCT
TATAATGGAAAACCAATTGTTAATTCAGTAAATGGAGAAGATGAGGTATTAGAAAAGATATTACCTTTGGTAAAAAAATA
TGGAGCAAGTGTTGTGGGACTTACATTAGATAAAAGAGGTATACCTGCTACTGCTGATGAGAGATTTAAGATAGCAGAGA
AGATAGTAAAAAAAGCTAAAGAGTATGGGATAGAGAAACATAATGTATTTATTGATTGCTTAGTATTAACAGCTTCTGCA
CAACAGGCGGAAGTGAAAGAAACATTAAAAGCATTAAGACGAGTTAAAGAAGAATTAGGTGTAAAAACATTACTAGGAGT
TTCAAATATATCTTTTGGTTTACCGTGCAGAGAACTAATAAATGAAACATTTCTAGCAATGGCAATAGCTAATGGATTAG
ATCTTCCAATAATGAATCCTAATAGTACTGGTATGATGGCTGTAATAAATTCATATAATGTACTGGCTAATATAGACAAA
AGATCAGAGAAATATATATCTATATATGGAAATGTAAAAGTTGAAAGAGGATTAAGAGATACTTCTAAAAAGAATATTGA
AGTAATTAAAAGTGAAAATATTGATTTAAAATATATAGTGATAAAAGGATTAAAGGATGAAGCAGAAGAGGCTACAAAAC
ATATTTTAAAAACAATGAATGAACTTAGTGTAGTTAATGAGATATTAATACCAGCACTAGATGAAGTTGGATTAAAATAT
GAAAAAGGTGAAATATTCTTGCCACAATTAATACAATGTGCAGAAACTGTTAAAAAGGCTTTTGAAGTTATAAAAAAGAG
ATTAGTTATTAACAAATCAAATAGTATATCTAAAGGCAAGATTGTATTAGCAACTGTAAAAGAGGATATTCATGATATAG
GAAAAAATATAGTTAAAGTAATTCTTGAGAATTATGGATATCAAATAATTGATTTAGGCAAAGATGTATCAGTAGAAACT
GTTGTAGATGTTTCTTTGAAAAATGATATTAAATTAGTTGGTCTTAGTGCTTTAATGACAACAACGTTAAAAAGTATGGA
AGAAACAATTAAGGGACTAAGGGAGAATGGATATAAAGGAAAGGTATTTGTAGGTGGAGCAGTATTGACATCAGAGTATG
CTAAACAAATAGGTGCTGATTATTATGCCAAAGATGCCAGAGAATCAGTTGAAATAGCTAAAATAGTATTGAATAATTAG

Upstream 100 bases:

>100_bases
ATAATAGGTGTTATTCCAAAGGAAAAAGAGGAAAAACAAAGAGGATGTGAAGTTTGTAAGAACTATAATAATTGTAGTTT
TAGAAGAGAGGGGAATAATT

Downstream 100 bases:

>100_bases
TAAAAATATAAGTTTTCTTGAATTGAGAAAGATATTTCTTAATTTAAGAAAGCTTTTTTATTTTGTTATGTTTTATTAAA
GTGTTAACATAAATCATTTC

Product: 5-methyltetrahydrofolate--homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 799; Mature: 798

Protein sequence:

>799_residues
MGLKEFIKDNILVFDGAMGTMLQKEGLKLGENPEIFNIEESEKVKKIHEEYIKNGAMIITSNTFGANELKLENTGYTVEE
IINAAISLAKEATGNEKVYIALDIGPIGELLEPMGTLSFDRAYEIFKRQIVQGEKSGADLILIETMTDLYEAKAALLAAK
ENTSLPVFCTMSFDENGRTFTGCTPESMAITLEGIGADAVGINCSLGPKELLPIVKRIKKATNLPIMTQPNAGLPKLSFG
EAIYDITSDEFEYWVNEFVKEGVSIIGGCCGTTPKFIKRLRVLADNNKRIIRDKIEFSAVCTPSNVVKIEGVKVIGERIN
PTGKKLFKEALKNNDIDYILKQAIEQIEAGAEILDVNVGLPEINEKEMMKKVVKEIQGIIDAPLQIDSSNIAAIETGLRA
YNGKPIVNSVNGEDEVLEKILPLVKKYGASVVGLTLDKRGIPATADERFKIAEKIVKKAKEYGIEKHNVFIDCLVLTASA
QQAEVKETLKALRRVKEELGVKTLLGVSNISFGLPCRELINETFLAMAIANGLDLPIMNPNSTGMMAVINSYNVLANIDK
RSEKYISIYGNVKVERGLRDTSKKNIEVIKSENIDLKYIVIKGLKDEAEEATKHILKTMNELSVVNEILIPALDEVGLKY
EKGEIFLPQLIQCAETVKKAFEVIKKRLVINKSNSISKGKIVLATVKEDIHDIGKNIVKVILENYGYQIIDLGKDVSVET
VVDVSLKNDIKLVGLSALMTTTLKSMEETIKGLRENGYKGKVFVGGAVLTSEYAKQIGADYYAKDARESVEIAKIVLNN

Sequences:

>Translated_799_residues
MGLKEFIKDNILVFDGAMGTMLQKEGLKLGENPEIFNIEESEKVKKIHEEYIKNGAMIITSNTFGANELKLENTGYTVEE
IINAAISLAKEATGNEKVYIALDIGPIGELLEPMGTLSFDRAYEIFKRQIVQGEKSGADLILIETMTDLYEAKAALLAAK
ENTSLPVFCTMSFDENGRTFTGCTPESMAITLEGIGADAVGINCSLGPKELLPIVKRIKKATNLPIMTQPNAGLPKLSFG
EAIYDITSDEFEYWVNEFVKEGVSIIGGCCGTTPKFIKRLRVLADNNKRIIRDKIEFSAVCTPSNVVKIEGVKVIGERIN
PTGKKLFKEALKNNDIDYILKQAIEQIEAGAEILDVNVGLPEINEKEMMKKVVKEIQGIIDAPLQIDSSNIAAIETGLRA
YNGKPIVNSVNGEDEVLEKILPLVKKYGASVVGLTLDKRGIPATADERFKIAEKIVKKAKEYGIEKHNVFIDCLVLTASA
QQAEVKETLKALRRVKEELGVKTLLGVSNISFGLPCRELINETFLAMAIANGLDLPIMNPNSTGMMAVINSYNVLANIDK
RSEKYISIYGNVKVERGLRDTSKKNIEVIKSENIDLKYIVIKGLKDEAEEATKHILKTMNELSVVNEILIPALDEVGLKY
EKGEIFLPQLIQCAETVKKAFEVIKKRLVINKSNSISKGKIVLATVKEDIHDIGKNIVKVILENYGYQIIDLGKDVSVET
VVDVSLKNDIKLVGLSALMTTTLKSMEETIKGLRENGYKGKVFVGGAVLTSEYAKQIGADYYAKDARESVEIAKIVLNN
>Mature_798_residues
GLKEFIKDNILVFDGAMGTMLQKEGLKLGENPEIFNIEESEKVKKIHEEYIKNGAMIITSNTFGANELKLENTGYTVEEI
INAAISLAKEATGNEKVYIALDIGPIGELLEPMGTLSFDRAYEIFKRQIVQGEKSGADLILIETMTDLYEAKAALLAAKE
NTSLPVFCTMSFDENGRTFTGCTPESMAITLEGIGADAVGINCSLGPKELLPIVKRIKKATNLPIMTQPNAGLPKLSFGE
AIYDITSDEFEYWVNEFVKEGVSIIGGCCGTTPKFIKRLRVLADNNKRIIRDKIEFSAVCTPSNVVKIEGVKVIGERINP
TGKKLFKEALKNNDIDYILKQAIEQIEAGAEILDVNVGLPEINEKEMMKKVVKEIQGIIDAPLQIDSSNIAAIETGLRAY
NGKPIVNSVNGEDEVLEKILPLVKKYGASVVGLTLDKRGIPATADERFKIAEKIVKKAKEYGIEKHNVFIDCLVLTASAQ
QAEVKETLKALRRVKEELGVKTLLGVSNISFGLPCRELINETFLAMAIANGLDLPIMNPNSTGMMAVINSYNVLANIDKR
SEKYISIYGNVKVERGLRDTSKKNIEVIKSENIDLKYIVIKGLKDEAEEATKHILKTMNELSVVNEILIPALDEVGLKYE
KGEIFLPQLIQCAETVKKAFEVIKKRLVINKSNSISKGKIVLATVKEDIHDIGKNIVKVILENYGYQIIDLGKDVSVETV
VDVSLKNDIKLVGLSALMTTTLKSMEETIKGLRENGYKGKVFVGGAVLTSEYAKQIGADYYAKDARESVEIAKIVLNN

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=896, Percent_Identity=29.4642857142857, Blast_Score=294, Evalue=2e-79,
Organism=Homo sapiens, GI157266337, Length=295, Percent_Identity=27.7966101694915, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI13162290, Length=287, Percent_Identity=27.5261324041812, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1790450, Length=884, Percent_Identity=29.185520361991, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI1786456, Length=309, Percent_Identity=23.9482200647249, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535405, Length=885, Percent_Identity=29.6045197740113, Blast_Score=301, Evalue=9e-82,
Organism=Saccharomyces cerevisiae, GI6324982, Length=325, Percent_Identity=28.3076923076923, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6322966, Length=321, Percent_Identity=25.5451713395639, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 87943; Mature: 87812

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKEFIKDNILVFDGAMGTMLQKEGLKLGENPEIFNIEESEKVKKIHEEYIKNGAMIIT
CCHHHHHHCCEEEEECCHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCEEEE
SNTFGANELKLENTGYTVEEIINAAISLAKEATGNEKVYIALDIGPIGELLEPMGTLSFD
ECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCHH
RAYEIFKRQIVQGEKSGADLILIETMTDLYEAKAALLAAKENTSLPVFCTMSFDENGRTF
HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHEECCCCCCEEEEEEEECCCCCEE
TGCTPESMAITLEGIGADAVGINCSLGPKELLPIVKRIKKATNLPIMTQPNAGLPKLSFG
ECCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
EAIYDITSDEFEYWVNEFVKEGVSIIGGCCGTTPKFIKRLRVLADNNKRIIRDKIEFSAV
CHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEE
CTPSNVVKIEGVKVIGERINPTGKKLFKEALKNNDIDYILKQAIEQIEAGAEILDVNVGL
ECCCCEEEECCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCC
PEINEKEMMKKVVKEIQGIIDAPLQIDSSNIAAIETGLRAYNGKPIVNSVNGEDEVLEKI
CCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCCEECCCCHHHHHHHH
LPLVKKYGASVVGLTLDKRGIPATADERFKIAEKIVKKAKEYGIEKHNVFIDCLVLTASA
HHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCC
QQAEVKETLKALRRVKEELGVKTLLGVSNISFGLPCRELINETFLAMAIANGLDLPIMNP
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECC
NSTGMMAVINSYNVLANIDKRSEKYISIYGNVKVERGLRDTSKKNIEVIKSENIDLKYIV
CCCCEEEEEECCHHEEECCCCCCCEEEEEECEEEECCCCCCCCCCEEEEECCCCCEEEEE
IKGLKDEAEEATKHILKTMNELSVVNEILIPALDEVGLKYEKGEIFLPQLIQCAETVKKA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEHHHHHHHHHHHHHH
FEVIKKRLVINKSNSISKGKIVLATVKEDIHDIGKNIVKVILENYGYQIIDLGKDVSVET
HHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEE
VVDVSLKNDIKLVGLSALMTTTLKSMEETIKGLRENGYKGKVFVGGAVLTSEYAKQIGAD
EEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCH
YYAKDARESVEIAKIVLNN
HHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
GLKEFIKDNILVFDGAMGTMLQKEGLKLGENPEIFNIEESEKVKKIHEEYIKNGAMIIT
CHHHHHHCCEEEEECCHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCEEEE
SNTFGANELKLENTGYTVEEIINAAISLAKEATGNEKVYIALDIGPIGELLEPMGTLSFD
ECCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCHH
RAYEIFKRQIVQGEKSGADLILIETMTDLYEAKAALLAAKENTSLPVFCTMSFDENGRTF
HHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHEECCCCCCEEEEEEEECCCCCEE
TGCTPESMAITLEGIGADAVGINCSLGPKELLPIVKRIKKATNLPIMTQPNAGLPKLSFG
ECCCCCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
EAIYDITSDEFEYWVNEFVKEGVSIIGGCCGTTPKFIKRLRVLADNNKRIIRDKIEFSAV
CHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEE
CTPSNVVKIEGVKVIGERINPTGKKLFKEALKNNDIDYILKQAIEQIEAGAEILDVNVGL
ECCCCEEEECCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCC
PEINEKEMMKKVVKEIQGIIDAPLQIDSSNIAAIETGLRAYNGKPIVNSVNGEDEVLEKI
CCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCCEECCCCHHHHHHHH
LPLVKKYGASVVGLTLDKRGIPATADERFKIAEKIVKKAKEYGIEKHNVFIDCLVLTASA
HHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCC
QQAEVKETLKALRRVKEELGVKTLLGVSNISFGLPCRELINETFLAMAIANGLDLPIMNP
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECC
NSTGMMAVINSYNVLANIDKRSEKYISIYGNVKVERGLRDTSKKNIEVIKSENIDLKYIV
CCCCEEEEEECCHHEEECCCCCCCEEEEEECEEEECCCCCCCCCCEEEEECCCCCEEEEE
IKGLKDEAEEATKHILKTMNELSVVNEILIPALDEVGLKYEKGEIFLPQLIQCAETVKKA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEHHHHHHHHHHHHHH
FEVIKKRLVINKSNSISKGKIVLATVKEDIHDIGKNIVKVILENYGYQIIDLGKDVSVET
HHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEE
VVDVSLKNDIKLVGLSALMTTTLKSMEETIKGLRENGYKGKVFVGGAVLTSEYAKQIGAD
EEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCH
YYAKDARESVEIAKIVLNN
HHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]