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The map label for this gene is hupL [C]

Identifier: 187934620

GI number: 187934620

Start: 2087188

End: 2088603

Strand: Reverse

Name: hupL [C]

Synonym: CLL_A2007

Alternate gene names: 187934620

Gene position: 2088603-2087188 (Counterclockwise)

Preceding gene: 187935390

Following gene: 187933519

Centisome position: 54.96

GC content: 31.71

Gene sequence:

>1416_bases
ATGGCTAAAATAGTAATTGATCCAGTAACAAGAATAAGTGGGTTATTAAAAATAGAAGTTGATGTGGAAAATAATAAAAT
AGTTGATGCAAAAAGCACAGGCTCACAATTTAGAGGTTTTGAAAAGATGTTTCAAGGAAGACCACCGTTAGACATTATAA
GATTAGCTCCTAGAATATGTGGAATATGTTCAACTAGCCATGCAATAGCAGCAACATTAGCACTTGAAAATGCACTTAAT
ATTACTCCAGATTTTAATGGAAAAGTAATACGTGATATTGCTCATGGATTTGAATTTCTTCAAAATCACTTAAGAACAAC
ATACTTTTTTGCATTTCCTGATTTCGTCAAAATAACAGCTAGTAATCCCTTGTATAAAGATGGTACAGATAAAGAATTAG
ATTACAGATTACCAGAAGATATAACTAAAAAAATTAATGAAGATTACTTGGAAGCAGTAAAATATAGCAGAGAATGTCAT
AGAGCAATAGCAGTTTTAGCTGGCAAAGCTCCTCATTGTCATGGGATTTGGGTAGGAGGTATAAGCACTTATATAGATGT
ACCAGAAAAAGAGTCTGTTAAGTACACAATTACAGTAGTTAAAGAATTTATTGAAAATAAGCTTTTAGAAGATGTAAAAA
TAATAGCACAATATTATAATGACTATTTTAATATGGGTAAAGGCTATGGAAATCTTATGGATTTTGGATTGTACAGTGAT
TATAAAGCTCCAATTAAATATACTGAAGCAAGTGCAATGATAAATGGAAAAAAAGAAAATATTGATGTGAATAATATAAC
AGAAAATGTTAAATATAGCTATTTAGATATACCAGGTGGTATGGTAATGCCAGGAGTAAGCTCCACTCCAGAACCCAATC
CATATAAACAAGACGCATATAGCTGGGTTGATGCAGCAAGATATAAAAATTATTCTATGGAAGGTGGACCTTTAGCAAGG
CAAATACTTAATGGATATTATGAAAATAAAATATCTGCAATGGATAGGATTTTAGCAAGGGTTTTAGAAAGTGTTAAAAT
TTGTGAAAGTATAATAGGATTAATTGATTTAATGAAATTAGGTCAAGCAGTTCAAGAAGAATGGCAAATTCCAAGCAAGG
TGAATGGTATTGGTTTAATAGGAGCACCTAGAGGCGCTCTTGGACATTGGTTATCTATTGAAAATAAGCAAGTATCAAAT
TATACGTTAATACCACCTTCAGCGTGGAATCTATCACCAACTGATAGTAATAATATAAAAGGACCTGTTGAAGCAGCACT
AATAGGAACAGAGATAAATGATATGAAAAATCCTGTTGAAATTGGGAGAATTGTAAGAAGTTTTGATCCATGCTCAAATT
GTGCTGCTCATGTGACTAGTGATCGATATGATCCCATTGTTATAAATATAGTATGA

Upstream 100 bases:

>100_bases
TGGCCAATAGGTGATAGGACGCCATGTATAGGATGCGCAAGAAAGGGATTTCCAGATGCAATGGAACCATTTACTAAATA
TGAAGGAGGCCAGTAGCAGT

Downstream 100 bases:

>100_bases
TAAGAATTTTTGGTGTTGGGAATGTTTTGCTTTGTGATGATGGGATTGGAGTTAAGGTATGTCAGTGTATAAAAGAGAAG
TTTAAAAGTTATAAAAGTAT

Product: [Ni/Fe] hydrogenase, large subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAKIVIDPVTRISGLLKIEVDVENNKIVDAKSTGSQFRGFEKMFQGRPPLDIIRLAPRICGICSTSHAIAATLALENALN
ITPDFNGKVIRDIAHGFEFLQNHLRTTYFFAFPDFVKITASNPLYKDGTDKELDYRLPEDITKKINEDYLEAVKYSRECH
RAIAVLAGKAPHCHGIWVGGISTYIDVPEKESVKYTITVVKEFIENKLLEDVKIIAQYYNDYFNMGKGYGNLMDFGLYSD
YKAPIKYTEASAMINGKKENIDVNNITENVKYSYLDIPGGMVMPGVSSTPEPNPYKQDAYSWVDAARYKNYSMEGGPLAR
QILNGYYENKISAMDRILARVLESVKICESIIGLIDLMKLGQAVQEEWQIPSKVNGIGLIGAPRGALGHWLSIENKQVSN
YTLIPPSAWNLSPTDSNNIKGPVEAALIGTEINDMKNPVEIGRIVRSFDPCSNCAAHVTSDRYDPIVINIV

Sequences:

>Translated_471_residues
MAKIVIDPVTRISGLLKIEVDVENNKIVDAKSTGSQFRGFEKMFQGRPPLDIIRLAPRICGICSTSHAIAATLALENALN
ITPDFNGKVIRDIAHGFEFLQNHLRTTYFFAFPDFVKITASNPLYKDGTDKELDYRLPEDITKKINEDYLEAVKYSRECH
RAIAVLAGKAPHCHGIWVGGISTYIDVPEKESVKYTITVVKEFIENKLLEDVKIIAQYYNDYFNMGKGYGNLMDFGLYSD
YKAPIKYTEASAMINGKKENIDVNNITENVKYSYLDIPGGMVMPGVSSTPEPNPYKQDAYSWVDAARYKNYSMEGGPLAR
QILNGYYENKISAMDRILARVLESVKICESIIGLIDLMKLGQAVQEEWQIPSKVNGIGLIGAPRGALGHWLSIENKQVSN
YTLIPPSAWNLSPTDSNNIKGPVEAALIGTEINDMKNPVEIGRIVRSFDPCSNCAAHVTSDRYDPIVINIV
>Mature_470_residues
AKIVIDPVTRISGLLKIEVDVENNKIVDAKSTGSQFRGFEKMFQGRPPLDIIRLAPRICGICSTSHAIAATLALENALNI
TPDFNGKVIRDIAHGFEFLQNHLRTTYFFAFPDFVKITASNPLYKDGTDKELDYRLPEDITKKINEDYLEAVKYSRECHR
AIAVLAGKAPHCHGIWVGGISTYIDVPEKESVKYTITVVKEFIENKLLEDVKIIAQYYNDYFNMGKGYGNLMDFGLYSDY
KAPIKYTEASAMINGKKENIDVNNITENVKYSYLDIPGGMVMPGVSSTPEPNPYKQDAYSWVDAARYKNYSMEGGPLARQ
ILNGYYENKISAMDRILARVLESVKICESIIGLIDLMKLGQAVQEEWQIPSKVNGIGLIGAPRGALGHWLSIENKQVSNY
TLIPPSAWNLSPTDSNNIKGPVEAALIGTEINDMKNPVEIGRIVRSFDPCSNCAAHVTSDRYDPIVINIV

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=571, Percent_Identity=28.0210157618214, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789368, Length=556, Percent_Identity=26.7985611510791, Blast_Score=206, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 52539; Mature: 52408

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVIDPVTRISGLLKIEVDVENNKIVDAKSTGSQFRGFEKMFQGRPPLDIIRLAPRIC
CCEEEECCHHHCCCEEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
GICSTSHAIAATLALENALNITPDFNGKVIRDIAHGFEFLQNHLRTTYFFAFPDFVKITA
HHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEC
SNPLYKDGTDKELDYRLPEDITKKINEDYLEAVKYSRECHRAIAVLAGKAPHCHGIWVGG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
ISTYIDVPEKESVKYTITVVKEFIENKLLEDVKIIAQYYNDYFNMGKGYGNLMDFGLYSD
HHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCC
YKAPIKYTEASAMINGKKENIDVNNITENVKYSYLDIPGGMVMPGVSSTPEPNPYKQDAY
CCCCCEEECHHHHCCCCCCCCCHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCCHHHHH
SWVDAARYKNYSMEGGPLARQILNGYYENKISAMDRILARVLESVKICESIIGLIDLMKL
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQAVQEEWQIPSKVNGIGLIGAPRGALGHWLSIENKQVSNYTLIPPSAWNLSPTDSNNIK
HHHHHHHCCCCCCCCCEEEEECCCCHHHCEEECCCCCCCCEEECCCCCCCCCCCCCCCCC
GPVEAALIGTEINDMKNPVEIGRIVRSFDPCSNCAAHVTSDRYDPIVINIV
CCHHHEEECCCHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEC
>Mature Secondary Structure 
AKIVIDPVTRISGLLKIEVDVENNKIVDAKSTGSQFRGFEKMFQGRPPLDIIRLAPRIC
CEEEECCHHHCCCEEEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
GICSTSHAIAATLALENALNITPDFNGKVIRDIAHGFEFLQNHLRTTYFFAFPDFVKITA
HHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEC
SNPLYKDGTDKELDYRLPEDITKKINEDYLEAVKYSRECHRAIAVLAGKAPHCHGIWVGG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
ISTYIDVPEKESVKYTITVVKEFIENKLLEDVKIIAQYYNDYFNMGKGYGNLMDFGLYSD
HHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCC
YKAPIKYTEASAMINGKKENIDVNNITENVKYSYLDIPGGMVMPGVSSTPEPNPYKQDAY
CCCCCEEECHHHHCCCCCCCCCHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCCHHHHH
SWVDAARYKNYSMEGGPLARQILNGYYENKISAMDRILARVLESVKICESIIGLIDLMKL
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQAVQEEWQIPSKVNGIGLIGAPRGALGHWLSIENKQVSNYTLIPPSAWNLSPTDSNNIK
HHHHHHHCCCCCCCCCEEEEECCCCHHHCEEECCCCCCCCEEECCCCCCCCCCCCCCCCC
GPVEAALIGTEINDMKNPVEIGRIVRSFDPCSNCAAHVTSDRYDPIVINIV
CCHHHEEECCCHHHCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]