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The map label for this gene is hupS [C]

Identifier: 187935390

GI number: 187935390

Start: 2088608

End: 2089549

Strand: Reverse

Name: hupS [C]

Synonym: CLL_A2008

Alternate gene names: 187935390

Gene position: 2089549-2088608 (Counterclockwise)

Preceding gene: 187934200

Following gene: 187934620

Centisome position: 54.98

GC content: 33.55

Gene sequence:

>942_bases
ATGTCTATAAATCATAAAAAAAGTAATAATGAAGTCAAAGATTACATGAAAAATGTAGGCTGTGCTAGGGGAGAAGGAAA
GCCTTTTAAATTTGCAAAAGAAATGATAGATAAAGCTACAGAAGAGATAAAAGCTAGAAATAAAGAAAAGATAAATGCAA
TTTGGTTAGAAACATCAGGATGTTTTGGTGAAGTTATATCATTGTTAAATTCTGAAATGCCAGATTTACCTTTTGTACTC
AAAAACCTTGTTAATATGACTTTTTTTGGAAGTATATGTGGGGATCAAGGAGAAAAAGCTTATGAAAGAATTTTAGATAC
ATTAAATAGTGATAAAGAATATATTTTATTGGTATGTGGAGCTATACCAATAAATGCAGATGGACTGTATACAGTTCTAG
CAACATATCAAGGAAGAAAAATTACGGCTATGGAGCTTGTAAAGCAAGCAGCACAAAAAGCAAAGTATATAATTTCAATT
GGAACTTGTGCTTGCTATGGAGGGCCAACAGCAGCTAAGCCTAATGTATCACAGGCCTTAAGTGTAAAAGAATATTTAAT
GAGAAATGATATTATAAACATTCCAGGATGCCCAGCAAATCCTGTTTGGACTTTGGGAACAGTTGGATATCTCATTAACT
ATGGAACAATAGATTTAGACGAAGAAGGTAGGCCGGTTGCTTTTTATGGCAAGACAATACATGAAATATGCCCAAAAAGA
AAATTTTTTGATAAAGGTATTTTTGCCAAGAAATTAGGTAATCCAGAATGTTTATATGAACTTGGATGCAAAGGACCTAT
AACTAAGGTGTATTGTCCTATAAGTAGATGGAATCAAAGCGATAATTGGCCAATAGGTGATAGGACGCCATGTATAGGAT
GCGCAAGAAAGGGATTTCCAGATGCAATGGAACCATTTACTAAATATGAAGGAGGCCAGTAG

Upstream 100 bases:

>100_bases
TTTTTATACAAATATCTATAGAACTAATAATGAAATGTAATAAATACTATATTTTTATAATATTAATTCTAAGAGTAGTA
CTAAGTAAGGAAAGAGATTT

Downstream 100 bases:

>100_bases
CAGTATGGCTAAAATAGTAATTGATCCAGTAACAAGAATAAGTGGGTTATTAAAAATAGAAGTTGATGTGGAAAATAATA
AAATAGTTGATGCAAAAAGC

Product: [Ni/Fe] hydrogenase, small subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSINHKKSNNEVKDYMKNVGCARGEGKPFKFAKEMIDKATEEIKARNKEKINAIWLETSGCFGEVISLLNSEMPDLPFVL
KNLVNMTFFGSICGDQGEKAYERILDTLNSDKEYILLVCGAIPINADGLYTVLATYQGRKITAMELVKQAAQKAKYIISI
GTCACYGGPTAAKPNVSQALSVKEYLMRNDIINIPGCPANPVWTLGTVGYLINYGTIDLDEEGRPVAFYGKTIHEICPKR
KFFDKGIFAKKLGNPECLYELGCKGPITKVYCPISRWNQSDNWPIGDRTPCIGCARKGFPDAMEPFTKYEGGQ

Sequences:

>Translated_313_residues
MSINHKKSNNEVKDYMKNVGCARGEGKPFKFAKEMIDKATEEIKARNKEKINAIWLETSGCFGEVISLLNSEMPDLPFVL
KNLVNMTFFGSICGDQGEKAYERILDTLNSDKEYILLVCGAIPINADGLYTVLATYQGRKITAMELVKQAAQKAKYIISI
GTCACYGGPTAAKPNVSQALSVKEYLMRNDIINIPGCPANPVWTLGTVGYLINYGTIDLDEEGRPVAFYGKTIHEICPKR
KFFDKGIFAKKLGNPECLYELGCKGPITKVYCPISRWNQSDNWPIGDRTPCIGCARKGFPDAMEPFTKYEGGQ
>Mature_312_residues
SINHKKSNNEVKDYMKNVGCARGEGKPFKFAKEMIDKATEEIKARNKEKINAIWLETSGCFGEVISLLNSEMPDLPFVLK
NLVNMTFFGSICGDQGEKAYERILDTLNSDKEYILLVCGAIPINADGLYTVLATYQGRKITAMELVKQAAQKAKYIISIG
TCACYGGPTAAKPNVSQALSVKEYLMRNDIINIPGCPANPVWTLGTVGYLINYGTIDLDEEGRPVAFYGKTIHEICPKRK
FFDKGIFAKKLGNPECLYELGCKGPITKVYCPISRWNQSDNWPIGDRTPCIGCARKGFPDAMEPFTKYEGGQ

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=296, Percent_Identity=34.4594594594595, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787206, Length=264, Percent_Identity=33.7121212121212, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 34722; Mature: 34590

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINHKKSNNEVKDYMKNVGCARGEGKPFKFAKEMIDKATEEIKARNKEKINAIWLETSG
CCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
CFGEVISLLNSEMPDLPFVLKNLVNMTFFGSICGDQGEKAYERILDTLNSDKEYILLVCG
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEC
AIPINADGLYTVLATYQGRKITAMELVKQAAQKAKYIISIGTCACYGGPTAAKPNVSQAL
CCEECCCCCEEEEECCCCCEEHHHHHHHHHHHHHHEEEEECCEEECCCCCCCCCCHHHHH
SVKEYLMRNDIINIPGCPANPVWTLGTVGYLINYGTIDLDEEGRPVAFYGKTIHEICPKR
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCEEEEECCHHHHHCCCH
KFFDKGIFAKKLGNPECLYELGCKGPITKVYCPISRWNQSDNWPIGDRTPCIGCARKGFP
HHHHCCHHHHHCCCHHHHHHCCCCCCCCEEEECHHHCCCCCCCCCCCCCCCHHHHCCCCC
DAMEPFTKYEGGQ
HHHHHHHHCCCCC
>Mature Secondary Structure 
SINHKKSNNEVKDYMKNVGCARGEGKPFKFAKEMIDKATEEIKARNKEKINAIWLETSG
CCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
CFGEVISLLNSEMPDLPFVLKNLVNMTFFGSICGDQGEKAYERILDTLNSDKEYILLVCG
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEC
AIPINADGLYTVLATYQGRKITAMELVKQAAQKAKYIISIGTCACYGGPTAAKPNVSQAL
CCEECCCCCEEEEECCCCCEEHHHHHHHHHHHHHHEEEEECCEEECCCCCCCCCCHHHHH
SVKEYLMRNDIINIPGCPANPVWTLGTVGYLINYGTIDLDEEGRPVAFYGKTIHEICPKR
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCEEEEECCHHHHHCCCH
KFFDKGIFAKKLGNPECLYELGCKGPITKVYCPISRWNQSDNWPIGDRTPCIGCARKGFP
HHHHCCHHHHHCCCHHHHHHCCCCCCCCEEEECHHHCCCCCCCCCCCCCCCHHHHCCCCC
DAMEPFTKYEGGQ
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]